Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5999 and Pf6N2E2_6000 are separated by 26 nucleotides Pf6N2E2_6000 and Pf6N2E2_6001 are separated by 89 nucleotides Pf6N2E2_6001 and Pf6N2E2_6002 are separated by 131 nucleotides
Pf6N2E2_5999 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 6,815,468 to 6,816,745
_5999
Pf6N2E2_6000 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 6,816,772 to 6,818,916
_6000
Pf6N2E2_6001 - Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85), at 6,819,006 to 6,819,740
_6001
Pf6N2E2_6002 - Magnesium and cobalt transport protein CorA, at 6,819,872 to 6,820,843
_6002
Position (kb)
6816
6817
6818
6819 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6816.100 kb on + strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.259 kb on + strand, within Pf6N2E2_5999 at 6816.260 kb on - strand, within Pf6N2E2_5999 at 6816.643 kb on + strand at 6816.868 kb on + strand at 6816.869 kb on - strand at 6816.869 kb on - strand at 6816.869 kb on - strand at 6817.056 kb on + strand, within Pf6N2E2_6000 at 6817.347 kb on + strand, within Pf6N2E2_6000 at 6817.601 kb on - strand, within Pf6N2E2_6000 at 6817.711 kb on + strand, within Pf6N2E2_6000 at 6817.712 kb on - strand, within Pf6N2E2_6000 at 6817.712 kb on - strand, within Pf6N2E2_6000 at 6817.768 kb on + strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.800 kb on - strand, within Pf6N2E2_6000 at 6818.298 kb on + strand, within Pf6N2E2_6000 at 6818.299 kb on - strand, within Pf6N2E2_6000 at 6818.299 kb on - strand, within Pf6N2E2_6000 at 6818.525 kb on - strand, within Pf6N2E2_6000 at 6818.525 kb on - strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.561 kb on - strand, within Pf6N2E2_6000 at 6818.587 kb on + strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.699 kb on + strand, within Pf6N2E2_6000 at 6818.836 kb on + strand at 6818.978 kb on - strand at 6818.998 kb on + strand at 6819.005 kb on + strand at 6819.005 kb on + strand at 6819.006 kb on - strand at 6819.006 kb on - strand at 6819.013 kb on + strand at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.136 kb on - strand, within Pf6N2E2_6001 at 6819.136 kb on - strand, within Pf6N2E2_6001 at 6819.186 kb on + strand, within Pf6N2E2_6001 at 6819.186 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.253 kb on - strand, within Pf6N2E2_6001 at 6819.523 kb on - strand, within Pf6N2E2_6001 at 6819.580 kb on - strand, within Pf6N2E2_6001 at 6819.580 kb on - strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.633 kb on - strand, within Pf6N2E2_6001 at 6819.675 kb on - strand at 6819.675 kb on - strand at 6819.873 kb on + strand at 6819.873 kb on + strand at 6819.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,816,100 + Pf6N2E2_5999 0.49 -0.6 6,816,258 - Pf6N2E2_5999 0.62 -0.8 6,816,258 - Pf6N2E2_5999 0.62 -0.8 6,816,258 - Pf6N2E2_5999 0.62 -2.8 6,816,258 - Pf6N2E2_5999 0.62 +0.6 6,816,259 + Pf6N2E2_5999 0.62 +0.8 6,816,260 - Pf6N2E2_5999 0.62 +0.3 6,816,643 + -0.2 6,816,868 + +0.3 6,816,869 - +1.9 6,816,869 - +0.3 6,816,869 - -0.4 6,817,056 + Pf6N2E2_6000 0.13 -0.7 6,817,347 + Pf6N2E2_6000 0.27 +0.6 6,817,601 - Pf6N2E2_6000 0.39 +1.8 6,817,711 + Pf6N2E2_6000 0.44 -0.8 6,817,712 - Pf6N2E2_6000 0.44 +0.3 6,817,712 - Pf6N2E2_6000 0.44 +1.4 6,817,768 + Pf6N2E2_6000 0.46 +1.8 6,817,769 - Pf6N2E2_6000 0.46 +0.0 6,817,769 - Pf6N2E2_6000 0.46 -1.0 6,817,769 - Pf6N2E2_6000 0.46 +0.8 6,817,769 - Pf6N2E2_6000 0.46 -0.1 6,817,769 - Pf6N2E2_6000 0.46 +0.4 6,817,800 - Pf6N2E2_6000 0.48 -0.8 6,818,298 + Pf6N2E2_6000 0.71 +1.2 6,818,299 - Pf6N2E2_6000 0.71 -0.3 6,818,299 - Pf6N2E2_6000 0.71 +0.8 6,818,525 - Pf6N2E2_6000 0.82 +0.7 6,818,525 - Pf6N2E2_6000 0.82 +0.1 6,818,560 + Pf6N2E2_6000 0.83 -0.6 6,818,560 + Pf6N2E2_6000 0.83 +0.0 6,818,560 + Pf6N2E2_6000 0.83 -0.1 6,818,561 - Pf6N2E2_6000 0.83 +0.4 6,818,587 + Pf6N2E2_6000 0.85 -0.5 6,818,588 - Pf6N2E2_6000 0.85 -1.1 6,818,588 - Pf6N2E2_6000 0.85 +1.3 6,818,588 - Pf6N2E2_6000 0.85 -0.5 6,818,588 - Pf6N2E2_6000 0.85 +0.2 6,818,699 + Pf6N2E2_6000 0.90 +0.8 6,818,836 + +0.1 6,818,978 - +2.0 6,818,998 + -0.5 6,819,005 + +1.5 6,819,005 + +1.0 6,819,006 - +0.6 6,819,006 - +2.1 6,819,013 + +0.8 6,819,135 + Pf6N2E2_6001 0.18 +1.3 6,819,135 + Pf6N2E2_6001 0.18 +0.6 6,819,135 + Pf6N2E2_6001 0.18 +1.8 6,819,135 + Pf6N2E2_6001 0.18 +1.6 6,819,136 - Pf6N2E2_6001 0.18 +1.9 6,819,136 - Pf6N2E2_6001 0.18 +0.8 6,819,186 + Pf6N2E2_6001 0.24 +2.1 6,819,186 + Pf6N2E2_6001 0.24 +1.8 6,819,252 + Pf6N2E2_6001 0.33 +3.0 6,819,252 + Pf6N2E2_6001 0.33 +1.8 6,819,252 + Pf6N2E2_6001 0.33 +2.1 6,819,253 - Pf6N2E2_6001 0.34 +2.8 6,819,523 - Pf6N2E2_6001 0.70 +0.6 6,819,580 - Pf6N2E2_6001 0.78 +2.1 6,819,580 - Pf6N2E2_6001 0.78 +2.4 6,819,630 + Pf6N2E2_6001 0.85 +1.2 6,819,630 + Pf6N2E2_6001 0.85 +1.7 6,819,630 + Pf6N2E2_6001 0.85 +1.3 6,819,630 + Pf6N2E2_6001 0.85 +1.7 6,819,630 + Pf6N2E2_6001 0.85 +1.4 6,819,630 + Pf6N2E2_6001 0.85 +1.1 6,819,630 + Pf6N2E2_6001 0.85 +2.2 6,819,630 + Pf6N2E2_6001 0.85 +0.9 6,819,631 - Pf6N2E2_6001 0.85 +1.8 6,819,631 - Pf6N2E2_6001 0.85 +1.5 6,819,631 - Pf6N2E2_6001 0.85 +2.9 6,819,631 - Pf6N2E2_6001 0.85 +2.4 6,819,633 - Pf6N2E2_6001 0.85 +1.9 6,819,675 - +2.9 6,819,675 - +1.8 6,819,873 + +1.1 6,819,873 + +1.5 6,819,874 - +0.4
Or see this region's nucleotide sequence