Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_597 and Pf6N2E2_598 are separated by 16 nucleotides Pf6N2E2_598 and Pf6N2E2_599 are separated by 93 nucleotides Pf6N2E2_599 and Pf6N2E2_600 overlap by 1 nucleotides Pf6N2E2_600 and Pf6N2E2_601 overlap by 4 nucleotides
Pf6N2E2_597 - FIG00953564: hypothetical protein, at 673,002 to 673,967
_597
Pf6N2E2_598 - hypothetical protein, at 673,984 to 674,145
_598
Pf6N2E2_599 - NAD kinase (EC 2.7.1.23), at 674,239 to 675,129
_599
Pf6N2E2_600 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 675,129 to 676,100
_600
Pf6N2E2_601 - GlpG protein (membrane protein of glp regulon), at 676,097 to 676,969
_601
Position (kb) 674
675
676 Strain fitness (log2 ratio) -2
-1
0
1 at 673.339 kb on + strand, within Pf6N2E2_597 at 673.378 kb on - strand, within Pf6N2E2_597 at 673.536 kb on - strand, within Pf6N2E2_597 at 673.555 kb on + strand, within Pf6N2E2_597 at 673.555 kb on + strand, within Pf6N2E2_597 at 673.556 kb on - strand, within Pf6N2E2_597 at 673.556 kb on - strand, within Pf6N2E2_597 at 673.603 kb on - strand, within Pf6N2E2_597 at 673.718 kb on - strand, within Pf6N2E2_597 at 673.718 kb on - strand, within Pf6N2E2_597 at 674.016 kb on - strand, within Pf6N2E2_598 at 674.178 kb on + strand at 674.197 kb on + strand at 675.423 kb on + strand, within Pf6N2E2_600 at 675.423 kb on + strand, within Pf6N2E2_600 at 675.424 kb on - strand at 675.526 kb on - strand, within Pf6N2E2_600 at 675.933 kb on + strand, within Pf6N2E2_600 at 675.988 kb on - strand, within Pf6N2E2_600
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 673,339 + Pf6N2E2_597 0.35 +0.1 673,378 - Pf6N2E2_597 0.39 +0.2 673,536 - Pf6N2E2_597 0.55 +0.1 673,555 + Pf6N2E2_597 0.57 -1.6 673,555 + Pf6N2E2_597 0.57 +1.1 673,556 - Pf6N2E2_597 0.57 -0.5 673,556 - Pf6N2E2_597 0.57 -1.1 673,603 - Pf6N2E2_597 0.62 -0.3 673,718 - Pf6N2E2_597 0.74 +0.8 673,718 - Pf6N2E2_597 0.74 +0.2 674,016 - Pf6N2E2_598 0.20 -2.3 674,178 + -0.6 674,197 + +0.1 675,423 + Pf6N2E2_600 0.30 +1.5 675,423 + Pf6N2E2_600 0.30 -0.5 675,424 - +0.5 675,526 - Pf6N2E2_600 0.41 -0.7 675,933 + Pf6N2E2_600 0.83 +1.1 675,988 - Pf6N2E2_600 0.88 -1.1
Or see this region's nucleotide sequence