Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5418 and Pf6N2E2_5419 are separated by 8 nucleotides Pf6N2E2_5419 and Pf6N2E2_5420 are separated by 60 nucleotides Pf6N2E2_5420 and Pf6N2E2_5421 are separated by 530 nucleotides
Pf6N2E2_5418 - FIG00957284: hypothetical protein, at 6,223,551 to 6,223,916
_5418
Pf6N2E2_5419 - DNA polymerase III chi subunit (EC 2.7.7.7), at 6,223,925 to 6,224,353
_5419
Pf6N2E2_5420 - Cytosol aminopeptidase PepA (EC 3.4.11.1), at 6,224,414 to 6,225,904
_5420
Pf6N2E2_5421 - FIG000988: Predicted permease, at 6,226,435 to 6,227,556
_5421
Position (kb)
6224
6225
6226 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6223.552 kb on + strand at 6223.553 kb on - strand at 6223.553 kb on - strand at 6223.553 kb on - strand at 6223.558 kb on + strand at 6223.558 kb on + strand at 6223.559 kb on - strand at 6223.559 kb on - strand at 6223.919 kb on + strand at 6223.937 kb on + strand at 6223.938 kb on - strand at 6224.247 kb on + strand, within Pf6N2E2_5419 at 6224.247 kb on + strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.334 kb on + strand at 6224.334 kb on + strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.355 kb on - strand at 6224.439 kb on + strand at 6224.641 kb on - strand, within Pf6N2E2_5420 at 6224.641 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.153 kb on + strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.204 kb on + strand, within Pf6N2E2_5420 at 6225.538 kb on - strand, within Pf6N2E2_5420 at 6225.722 kb on + strand, within Pf6N2E2_5420 at 6225.722 kb on + strand, within Pf6N2E2_5420 at 6225.722 kb on + strand, within Pf6N2E2_5420 at 6225.890 kb on + strand at 6225.891 kb on - strand at 6226.144 kb on + strand at 6226.145 kb on - strand at 6226.156 kb on + strand at 6226.156 kb on + strand at 6226.156 kb on + strand at 6226.157 kb on - strand at 6226.240 kb on + strand at 6226.273 kb on + strand at 6226.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,223,552 + +0.5 6,223,553 - +1.6 6,223,553 - -0.1 6,223,553 - +0.7 6,223,558 + -0.3 6,223,558 + -0.1 6,223,559 - -1.1 6,223,559 - -2.3 6,223,919 + -0.5 6,223,937 + +1.2 6,223,938 - -1.5 6,224,247 + Pf6N2E2_5419 0.75 -1.3 6,224,247 + Pf6N2E2_5419 0.75 -0.2 6,224,248 - Pf6N2E2_5419 0.75 -0.8 6,224,248 - Pf6N2E2_5419 0.75 -0.0 6,224,248 - Pf6N2E2_5419 0.75 -0.5 6,224,248 - Pf6N2E2_5419 0.75 +1.1 6,224,334 + +0.6 6,224,334 + -1.4 6,224,335 - -2.0 6,224,335 - -0.8 6,224,335 - +0.4 6,224,335 - +0.2 6,224,335 - +1.5 6,224,355 - -0.4 6,224,439 + -3.4 6,224,641 - Pf6N2E2_5420 0.15 -1.2 6,224,641 - Pf6N2E2_5420 0.15 +0.2 6,224,824 - Pf6N2E2_5420 0.27 -0.8 6,224,824 - Pf6N2E2_5420 0.27 -1.1 6,224,824 - Pf6N2E2_5420 0.27 -1.3 6,225,043 - Pf6N2E2_5420 0.42 +0.0 6,225,043 - Pf6N2E2_5420 0.42 -3.2 6,225,043 - Pf6N2E2_5420 0.42 -1.6 6,225,043 - Pf6N2E2_5420 0.42 -0.3 6,225,153 + Pf6N2E2_5420 0.50 +0.7 6,225,154 - Pf6N2E2_5420 0.50 -1.4 6,225,154 - Pf6N2E2_5420 0.50 -0.8 6,225,154 - Pf6N2E2_5420 0.50 -2.1 6,225,204 + Pf6N2E2_5420 0.53 -0.9 6,225,538 - Pf6N2E2_5420 0.75 -1.5 6,225,722 + Pf6N2E2_5420 0.88 -2.0 6,225,722 + Pf6N2E2_5420 0.88 -1.0 6,225,722 + Pf6N2E2_5420 0.88 +0.3 6,225,890 + -2.0 6,225,891 - -2.6 6,226,144 + +0.3 6,226,145 - -0.5 6,226,156 + +0.8 6,226,156 + +0.3 6,226,156 + -0.5 6,226,157 - -1.1 6,226,240 + +0.2 6,226,273 + -0.2 6,226,399 + +0.7
Or see this region's nucleotide sequence