Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5332 and Pf6N2E2_5333 are separated by 151 nucleotides Pf6N2E2_5333 and Pf6N2E2_5334 are separated by 131 nucleotides
Pf6N2E2_5332 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 6,127,271 to 6,128,260
_5332
Pf6N2E2_5333 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 6,128,412 to 6,129,548
_5333
Pf6N2E2_5334 - Oxidoreductase, aldo/keto reductase family, at 6,129,680 to 6,130,720
_5334
Position (kb)
6128
6129
6130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6127.449 kb on + strand, within Pf6N2E2_5332 at 6127.450 kb on - strand, within Pf6N2E2_5332 at 6127.450 kb on - strand, within Pf6N2E2_5332 at 6127.450 kb on - strand, within Pf6N2E2_5332 at 6127.450 kb on - strand, within Pf6N2E2_5332 at 6127.450 kb on - strand, within Pf6N2E2_5332 at 6127.547 kb on - strand, within Pf6N2E2_5332 at 6127.641 kb on + strand, within Pf6N2E2_5332 at 6127.641 kb on + strand, within Pf6N2E2_5332 at 6127.642 kb on - strand, within Pf6N2E2_5332 at 6127.642 kb on - strand, within Pf6N2E2_5332 at 6127.659 kb on + strand, within Pf6N2E2_5332 at 6127.659 kb on + strand, within Pf6N2E2_5332 at 6127.659 kb on + strand, within Pf6N2E2_5332 at 6127.660 kb on - strand, within Pf6N2E2_5332 at 6127.660 kb on - strand, within Pf6N2E2_5332 at 6127.660 kb on - strand, within Pf6N2E2_5332 at 6127.719 kb on + strand, within Pf6N2E2_5332 at 6127.719 kb on + strand, within Pf6N2E2_5332 at 6127.719 kb on + strand, within Pf6N2E2_5332 at 6127.719 kb on + strand, within Pf6N2E2_5332 at 6127.719 kb on + strand, within Pf6N2E2_5332 at 6127.720 kb on - strand, within Pf6N2E2_5332 at 6127.720 kb on - strand, within Pf6N2E2_5332 at 6127.720 kb on - strand, within Pf6N2E2_5332 at 6127.720 kb on - strand, within Pf6N2E2_5332 at 6127.725 kb on + strand, within Pf6N2E2_5332 at 6127.726 kb on - strand, within Pf6N2E2_5332 at 6127.726 kb on - strand, within Pf6N2E2_5332 at 6127.805 kb on + strand, within Pf6N2E2_5332 at 6127.805 kb on + strand, within Pf6N2E2_5332 at 6127.806 kb on - strand, within Pf6N2E2_5332 at 6127.848 kb on + strand, within Pf6N2E2_5332 at 6127.849 kb on - strand, within Pf6N2E2_5332 at 6128.032 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.060 kb on + strand, within Pf6N2E2_5332 at 6128.061 kb on - strand, within Pf6N2E2_5332 at 6128.061 kb on - strand, within Pf6N2E2_5332 at 6128.061 kb on - strand, within Pf6N2E2_5332 at 6128.061 kb on - strand, within Pf6N2E2_5332 at 6128.061 kb on - strand, within Pf6N2E2_5332 at 6128.061 kb on - strand, within Pf6N2E2_5332 at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.260 kb on + strand at 6128.261 kb on - strand at 6128.261 kb on - strand at 6128.261 kb on - strand at 6128.261 kb on - strand at 6128.261 kb on - strand at 6128.261 kb on - strand at 6128.261 kb on - strand at 6128.266 kb on + strand at 6128.266 kb on + strand at 6128.266 kb on + strand at 6128.266 kb on + strand at 6128.266 kb on + strand at 6128.266 kb on + strand at 6128.267 kb on - strand at 6128.267 kb on - strand at 6128.267 kb on - strand at 6128.267 kb on - strand at 6128.283 kb on + strand at 6128.283 kb on + strand at 6128.284 kb on - strand at 6128.366 kb on + strand at 6128.464 kb on + strand at 6128.464 kb on + strand at 6128.464 kb on + strand at 6128.592 kb on + strand, within Pf6N2E2_5333 at 6128.593 kb on - strand, within Pf6N2E2_5333 at 6128.619 kb on + strand, within Pf6N2E2_5333 at 6128.620 kb on - strand, within Pf6N2E2_5333 at 6128.703 kb on + strand, within Pf6N2E2_5333 at 6128.742 kb on + strand, within Pf6N2E2_5333 at 6128.742 kb on + strand, within Pf6N2E2_5333 at 6128.742 kb on + strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.856 kb on + strand, within Pf6N2E2_5333 at 6128.856 kb on + strand, within Pf6N2E2_5333 at 6128.856 kb on + strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6129.114 kb on + strand, within Pf6N2E2_5333 at 6129.131 kb on - strand, within Pf6N2E2_5333 at 6129.204 kb on + strand, within Pf6N2E2_5333 at 6129.204 kb on + strand, within Pf6N2E2_5333 at 6129.205 kb on - strand, within Pf6N2E2_5333 at 6129.205 kb on - strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.376 kb on - strand, within Pf6N2E2_5333 at 6129.498 kb on + strand at 6129.499 kb on - strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.891 kb on + strand, within Pf6N2E2_5334 at 6129.891 kb on + strand, within Pf6N2E2_5334 at 6129.892 kb on - strand, within Pf6N2E2_5334 at 6129.928 kb on - strand, within Pf6N2E2_5334 at 6129.945 kb on + strand, within Pf6N2E2_5334 at 6129.945 kb on + strand, within Pf6N2E2_5334 at 6129.945 kb on + strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.988 kb on - strand, within Pf6N2E2_5334 at 6130.098 kb on + strand, within Pf6N2E2_5334 at 6130.098 kb on + strand, within Pf6N2E2_5334 at 6130.098 kb on + strand, within Pf6N2E2_5334 at 6130.335 kb on + strand, within Pf6N2E2_5334 at 6130.335 kb on + strand, within Pf6N2E2_5334 at 6130.336 kb on - strand, within Pf6N2E2_5334 at 6130.348 kb on - strand, within Pf6N2E2_5334 at 6130.348 kb on - strand, within Pf6N2E2_5334 at 6130.431 kb on + strand, within Pf6N2E2_5334 at 6130.432 kb on - strand, within Pf6N2E2_5334 at 6130.438 kb on + strand, within Pf6N2E2_5334 at 6130.506 kb on + strand, within Pf6N2E2_5334 at 6130.506 kb on + strand, within Pf6N2E2_5334 at 6130.507 kb on - strand, within Pf6N2E2_5334 at 6130.518 kb on + strand, within Pf6N2E2_5334 at 6130.518 kb on + strand, within Pf6N2E2_5334 at 6130.518 kb on + strand, within Pf6N2E2_5334
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,127,449 + Pf6N2E2_5332 0.18 +0.2 6,127,450 - Pf6N2E2_5332 0.18 +0.3 6,127,450 - Pf6N2E2_5332 0.18 -2.6 6,127,450 - Pf6N2E2_5332 0.18 +0.6 6,127,450 - Pf6N2E2_5332 0.18 -0.4 6,127,450 - Pf6N2E2_5332 0.18 -0.4 6,127,547 - Pf6N2E2_5332 0.28 +0.6 6,127,641 + Pf6N2E2_5332 0.37 +0.1 6,127,641 + Pf6N2E2_5332 0.37 +0.3 6,127,642 - Pf6N2E2_5332 0.37 -0.2 6,127,642 - Pf6N2E2_5332 0.37 -0.2 6,127,659 + Pf6N2E2_5332 0.39 +1.0 6,127,659 + Pf6N2E2_5332 0.39 -1.3 6,127,659 + Pf6N2E2_5332 0.39 +0.1 6,127,660 - Pf6N2E2_5332 0.39 -0.5 6,127,660 - Pf6N2E2_5332 0.39 -1.4 6,127,660 - Pf6N2E2_5332 0.39 -0.7 6,127,719 + Pf6N2E2_5332 0.45 +0.9 6,127,719 + Pf6N2E2_5332 0.45 +0.3 6,127,719 + Pf6N2E2_5332 0.45 +0.7 6,127,719 + Pf6N2E2_5332 0.45 +0.6 6,127,719 + Pf6N2E2_5332 0.45 -0.2 6,127,720 - Pf6N2E2_5332 0.45 -0.8 6,127,720 - Pf6N2E2_5332 0.45 -0.4 6,127,720 - Pf6N2E2_5332 0.45 +0.7 6,127,720 - Pf6N2E2_5332 0.45 -0.0 6,127,725 + Pf6N2E2_5332 0.46 +0.5 6,127,726 - Pf6N2E2_5332 0.46 -0.9 6,127,726 - Pf6N2E2_5332 0.46 -0.1 6,127,805 + Pf6N2E2_5332 0.54 +0.5 6,127,805 + Pf6N2E2_5332 0.54 -0.8 6,127,806 - Pf6N2E2_5332 0.54 -2.5 6,127,848 + Pf6N2E2_5332 0.58 -0.2 6,127,849 - Pf6N2E2_5332 0.58 +0.6 6,128,032 + Pf6N2E2_5332 0.77 +0.4 6,128,060 + Pf6N2E2_5332 0.80 -0.6 6,128,060 + Pf6N2E2_5332 0.80 +1.2 6,128,060 + Pf6N2E2_5332 0.80 +0.9 6,128,060 + Pf6N2E2_5332 0.80 +1.2 6,128,060 + Pf6N2E2_5332 0.80 +0.4 6,128,060 + Pf6N2E2_5332 0.80 -0.4 6,128,060 + Pf6N2E2_5332 0.80 -0.6 6,128,061 - Pf6N2E2_5332 0.80 +0.1 6,128,061 - Pf6N2E2_5332 0.80 +0.9 6,128,061 - Pf6N2E2_5332 0.80 -1.4 6,128,061 - Pf6N2E2_5332 0.80 -0.1 6,128,061 - Pf6N2E2_5332 0.80 +0.5 6,128,061 - Pf6N2E2_5332 0.80 -0.4 6,128,260 + +0.2 6,128,260 + +0.4 6,128,260 + +1.1 6,128,260 + -1.0 6,128,260 + -0.5 6,128,260 + +0.6 6,128,260 + -1.5 6,128,260 + -2.7 6,128,261 - +0.3 6,128,261 - +0.2 6,128,261 - +0.8 6,128,261 - +0.7 6,128,261 - -0.7 6,128,261 - -0.4 6,128,261 - -0.6 6,128,266 + -0.3 6,128,266 + +1.0 6,128,266 + -0.2 6,128,266 + +1.0 6,128,266 + +1.2 6,128,266 + -1.5 6,128,267 - +0.5 6,128,267 - +0.3 6,128,267 - -0.1 6,128,267 - +1.4 6,128,283 + +1.3 6,128,283 + -0.3 6,128,284 - -1.3 6,128,366 + +0.6 6,128,464 + -1.2 6,128,464 + -0.2 6,128,464 + +0.5 6,128,592 + Pf6N2E2_5333 0.16 +0.8 6,128,593 - Pf6N2E2_5333 0.16 -1.7 6,128,619 + Pf6N2E2_5333 0.18 -0.0 6,128,620 - Pf6N2E2_5333 0.18 +1.2 6,128,703 + Pf6N2E2_5333 0.26 -1.5 6,128,742 + Pf6N2E2_5333 0.29 -0.3 6,128,742 + Pf6N2E2_5333 0.29 +0.9 6,128,742 + Pf6N2E2_5333 0.29 +0.3 6,128,743 - Pf6N2E2_5333 0.29 -0.2 6,128,743 - Pf6N2E2_5333 0.29 +1.8 6,128,743 - Pf6N2E2_5333 0.29 +0.4 6,128,743 - Pf6N2E2_5333 0.29 -1.0 6,128,856 + Pf6N2E2_5333 0.39 +0.2 6,128,856 + Pf6N2E2_5333 0.39 +1.2 6,128,856 + Pf6N2E2_5333 0.39 +1.2 6,128,857 - Pf6N2E2_5333 0.39 +0.2 6,128,857 - Pf6N2E2_5333 0.39 -2.4 6,128,857 - Pf6N2E2_5333 0.39 +0.1 6,128,857 - Pf6N2E2_5333 0.39 -0.0 6,128,857 - Pf6N2E2_5333 0.39 +0.5 6,128,857 - Pf6N2E2_5333 0.39 -0.3 6,129,114 + Pf6N2E2_5333 0.62 +1.4 6,129,131 - Pf6N2E2_5333 0.63 -0.6 6,129,204 + Pf6N2E2_5333 0.70 +0.4 6,129,204 + Pf6N2E2_5333 0.70 +0.8 6,129,205 - Pf6N2E2_5333 0.70 -0.6 6,129,205 - Pf6N2E2_5333 0.70 +1.2 6,129,375 + Pf6N2E2_5333 0.85 -0.2 6,129,375 + Pf6N2E2_5333 0.85 +0.0 6,129,375 + Pf6N2E2_5333 0.85 -0.8 6,129,375 + Pf6N2E2_5333 0.85 +1.9 6,129,376 - Pf6N2E2_5333 0.85 +0.1 6,129,498 + +0.9 6,129,499 - +0.3 6,129,622 + -0.1 6,129,622 + +0.6 6,129,622 + +0.5 6,129,622 + +0.5 6,129,622 + +0.4 6,129,622 + -0.7 6,129,891 + Pf6N2E2_5334 0.20 -1.2 6,129,891 + Pf6N2E2_5334 0.20 -0.2 6,129,892 - Pf6N2E2_5334 0.20 +0.3 6,129,928 - Pf6N2E2_5334 0.24 +0.1 6,129,945 + Pf6N2E2_5334 0.25 -1.7 6,129,945 + Pf6N2E2_5334 0.25 -0.2 6,129,945 + Pf6N2E2_5334 0.25 +0.8 6,129,946 - Pf6N2E2_5334 0.26 -2.1 6,129,946 - +0.2 6,129,946 - Pf6N2E2_5334 0.26 -1.2 6,129,946 - Pf6N2E2_5334 0.26 -0.1 6,129,946 - Pf6N2E2_5334 0.26 -3.0 6,129,946 - Pf6N2E2_5334 0.26 -0.8 6,129,988 - Pf6N2E2_5334 0.30 -0.2 6,130,098 + Pf6N2E2_5334 0.40 +0.8 6,130,098 + Pf6N2E2_5334 0.40 +0.2 6,130,098 + Pf6N2E2_5334 0.40 +0.4 6,130,335 + Pf6N2E2_5334 0.63 -0.1 6,130,335 + Pf6N2E2_5334 0.63 +1.5 6,130,336 - Pf6N2E2_5334 0.63 -0.4 6,130,348 - Pf6N2E2_5334 0.64 -1.7 6,130,348 - Pf6N2E2_5334 0.64 +0.2 6,130,431 + Pf6N2E2_5334 0.72 +0.1 6,130,432 - Pf6N2E2_5334 0.72 +0.8 6,130,438 + Pf6N2E2_5334 0.73 +0.2 6,130,506 + Pf6N2E2_5334 0.79 -0.6 6,130,506 + Pf6N2E2_5334 0.79 +0.7 6,130,507 - Pf6N2E2_5334 0.79 -2.1 6,130,518 + Pf6N2E2_5334 0.80 -0.8 6,130,518 + Pf6N2E2_5334 0.80 +0.8 6,130,518 + Pf6N2E2_5334 0.80 -0.1
Or see this region's nucleotide sequence