Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5300 and Pf6N2E2_5301 are separated by 74 nucleotides Pf6N2E2_5301 and Pf6N2E2_5302 are separated by 315 nucleotides Pf6N2E2_5302 and Pf6N2E2_5303 overlap by 4 nucleotides Pf6N2E2_5303 and Pf6N2E2_5304 are separated by 506 nucleotides
Pf6N2E2_5300 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 6,094,403 to 6,095,794
_5300
Pf6N2E2_5301 - FIG00957554: hypothetical protein, at 6,095,869 to 6,096,129
_5301
Pf6N2E2_5302 - FIG00954263: hypothetical protein, at 6,096,445 to 6,096,945
_5302
Pf6N2E2_5303 - FIG00954638: hypothetical protein, at 6,096,942 to 6,097,259
_5303
Pf6N2E2_5304 - ribosomal subunit interface protein, putative, at 6,097,766 to 6,098,176
_5304
Position (kb)
6096
6097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6095.558 kb on + strand, within Pf6N2E2_5300 at 6095.558 kb on + strand, within Pf6N2E2_5300 at 6095.564 kb on + strand, within Pf6N2E2_5300 at 6095.637 kb on + strand, within Pf6N2E2_5300 at 6095.694 kb on + strand at 6095.786 kb on + strand at 6095.786 kb on + strand at 6095.789 kb on + strand at 6095.792 kb on + strand at 6095.793 kb on - strand at 6095.793 kb on - strand at 6095.829 kb on + strand at 6095.829 kb on + strand at 6095.830 kb on - strand at 6095.830 kb on - strand at 6095.830 kb on - strand at 6095.830 kb on - strand at 6095.830 kb on - strand at 6095.866 kb on + strand at 6095.866 kb on + strand at 6095.871 kb on - strand at 6095.957 kb on + strand, within Pf6N2E2_5301 at 6095.957 kb on + strand, within Pf6N2E2_5301 at 6095.957 kb on + strand, within Pf6N2E2_5301 at 6095.958 kb on - strand, within Pf6N2E2_5301 at 6095.958 kb on - strand, within Pf6N2E2_5301 at 6095.958 kb on - strand, within Pf6N2E2_5301 at 6095.958 kb on - strand, within Pf6N2E2_5301 at 6095.958 kb on - strand, within Pf6N2E2_5301 at 6095.958 kb on - strand, within Pf6N2E2_5301 at 6096.025 kb on + strand, within Pf6N2E2_5301 at 6096.026 kb on - strand, within Pf6N2E2_5301 at 6096.026 kb on - strand, within Pf6N2E2_5301 at 6096.112 kb on + strand at 6096.112 kb on + strand at 6096.112 kb on + strand at 6096.112 kb on + strand at 6096.112 kb on + strand at 6096.113 kb on - strand at 6096.113 kb on - strand at 6096.113 kb on - strand at 6096.113 kb on - strand at 6096.113 kb on - strand at 6096.198 kb on - strand at 6096.231 kb on + strand at 6096.231 kb on + strand at 6096.232 kb on - strand at 6096.232 kb on - strand at 6096.236 kb on + strand at 6096.236 kb on + strand at 6096.430 kb on + strand at 6096.431 kb on - strand at 6096.431 kb on - strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.439 kb on + strand at 6096.440 kb on - strand at 6096.440 kb on - strand at 6096.440 kb on - strand at 6096.440 kb on - strand at 6096.440 kb on - strand at 6096.676 kb on + strand, within Pf6N2E2_5302 at 6096.676 kb on + strand, within Pf6N2E2_5302 at 6096.676 kb on + strand, within Pf6N2E2_5302 at 6096.677 kb on - strand, within Pf6N2E2_5302 at 6096.677 kb on - strand, within Pf6N2E2_5302 at 6096.677 kb on - strand, within Pf6N2E2_5302 at 6096.677 kb on - strand, within Pf6N2E2_5302 at 6096.677 kb on - strand, within Pf6N2E2_5302 at 6096.715 kb on + strand, within Pf6N2E2_5302 at 6096.715 kb on + strand, within Pf6N2E2_5302 at 6096.715 kb on + strand, within Pf6N2E2_5302 at 6096.716 kb on - strand, within Pf6N2E2_5302 at 6096.716 kb on - strand, within Pf6N2E2_5302 at 6096.716 kb on - strand, within Pf6N2E2_5302 at 6096.716 kb on - strand, within Pf6N2E2_5302 at 6096.793 kb on + strand, within Pf6N2E2_5302 at 6096.793 kb on + strand, within Pf6N2E2_5302 at 6096.793 kb on + strand, within Pf6N2E2_5302 at 6096.793 kb on + strand, within Pf6N2E2_5302 at 6096.793 kb on + strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.794 kb on - strand, within Pf6N2E2_5302 at 6096.845 kb on - strand, within Pf6N2E2_5302 at 6096.943 kb on + strand at 6096.943 kb on + strand at 6097.213 kb on + strand, within Pf6N2E2_5303 at 6097.419 kb on + strand at 6097.420 kb on - strand at 6097.420 kb on - strand at 6097.429 kb on + strand at 6097.429 kb on + strand at 6097.429 kb on + strand at 6097.429 kb on + strand at 6097.430 kb on - strand at 6097.430 kb on - strand at 6097.430 kb on - strand at 6097.529 kb on + strand at 6097.573 kb on - strand at 6097.637 kb on + strand at 6097.638 kb on - strand at 6097.651 kb on + strand at 6097.651 kb on + strand at 6097.651 kb on + strand at 6097.651 kb on + strand at 6097.651 kb on + strand at 6097.652 kb on - strand at 6097.700 kb on + strand at 6097.744 kb on + strand at 6097.745 kb on - strand at 6097.753 kb on + strand at 6097.765 kb on + strand at 6097.765 kb on + strand at 6097.765 kb on + strand at 6097.766 kb on - strand at 6097.766 kb on - strand at 6097.795 kb on + strand at 6097.796 kb on - strand at 6097.796 kb on - strand at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.831 kb on + strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.832 kb on - strand, within Pf6N2E2_5304 at 6097.834 kb on + strand, within Pf6N2E2_5304 at 6097.834 kb on + strand, within Pf6N2E2_5304 at 6097.835 kb on - strand, within Pf6N2E2_5304 at 6097.835 kb on - strand, within Pf6N2E2_5304 at 6097.835 kb on - strand, within Pf6N2E2_5304
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,095,558 + Pf6N2E2_5300 0.83 +0.1 6,095,558 + Pf6N2E2_5300 0.83 -0.4 6,095,564 + Pf6N2E2_5300 0.83 -2.1 6,095,637 + Pf6N2E2_5300 0.89 -1.0 6,095,694 + -0.2 6,095,786 + -3.2 6,095,786 + -0.6 6,095,789 + -1.9 6,095,792 + +0.7 6,095,793 - +1.1 6,095,793 - +0.3 6,095,829 + -0.9 6,095,829 + -0.2 6,095,830 - -0.7 6,095,830 - -0.2 6,095,830 - -1.4 6,095,830 - +0.4 6,095,830 - +0.1 6,095,866 + -1.8 6,095,866 + -1.4 6,095,871 - +1.1 6,095,957 + Pf6N2E2_5301 0.34 +0.2 6,095,957 + Pf6N2E2_5301 0.34 +0.1 6,095,957 + Pf6N2E2_5301 0.34 +0.0 6,095,958 - Pf6N2E2_5301 0.34 +0.8 6,095,958 - Pf6N2E2_5301 0.34 -0.1 6,095,958 - Pf6N2E2_5301 0.34 +0.7 6,095,958 - Pf6N2E2_5301 0.34 -0.4 6,095,958 - Pf6N2E2_5301 0.34 -0.3 6,095,958 - Pf6N2E2_5301 0.34 -0.6 6,096,025 + Pf6N2E2_5301 0.60 +1.8 6,096,026 - Pf6N2E2_5301 0.60 +1.5 6,096,026 - Pf6N2E2_5301 0.60 -0.2 6,096,112 + -0.9 6,096,112 + -0.8 6,096,112 + +0.9 6,096,112 + +0.2 6,096,112 + +0.4 6,096,113 - +1.0 6,096,113 - -1.2 6,096,113 - +0.4 6,096,113 - +0.7 6,096,113 - +0.9 6,096,198 - -0.9 6,096,231 + -0.9 6,096,231 + -0.6 6,096,232 - +0.6 6,096,232 - +0.2 6,096,236 + -0.8 6,096,236 + -0.4 6,096,430 + -0.8 6,096,431 - +0.1 6,096,431 - +0.0 6,096,439 + +0.2 6,096,439 + +1.0 6,096,439 + +0.7 6,096,439 + +0.1 6,096,439 + -0.6 6,096,439 + +0.9 6,096,439 + -0.3 6,096,439 + +0.2 6,096,440 - +0.0 6,096,440 - -0.1 6,096,440 - -0.2 6,096,440 - -0.4 6,096,440 - +0.4 6,096,676 + Pf6N2E2_5302 0.46 +1.1 6,096,676 + Pf6N2E2_5302 0.46 +0.1 6,096,676 + Pf6N2E2_5302 0.46 -1.4 6,096,677 - Pf6N2E2_5302 0.46 +0.6 6,096,677 - Pf6N2E2_5302 0.46 +1.5 6,096,677 - Pf6N2E2_5302 0.46 -0.1 6,096,677 - Pf6N2E2_5302 0.46 +0.4 6,096,677 - Pf6N2E2_5302 0.46 +1.0 6,096,715 + Pf6N2E2_5302 0.54 +0.9 6,096,715 + Pf6N2E2_5302 0.54 +1.1 6,096,715 + Pf6N2E2_5302 0.54 +0.5 6,096,716 - Pf6N2E2_5302 0.54 -0.8 6,096,716 - Pf6N2E2_5302 0.54 +0.5 6,096,716 - Pf6N2E2_5302 0.54 +1.1 6,096,716 - Pf6N2E2_5302 0.54 +1.4 6,096,793 + Pf6N2E2_5302 0.69 +0.5 6,096,793 + Pf6N2E2_5302 0.69 +0.7 6,096,793 + Pf6N2E2_5302 0.69 +2.2 6,096,793 + Pf6N2E2_5302 0.69 +0.1 6,096,793 + Pf6N2E2_5302 0.69 -0.0 6,096,794 - Pf6N2E2_5302 0.70 +0.3 6,096,794 - Pf6N2E2_5302 0.70 -2.5 6,096,794 - Pf6N2E2_5302 0.70 +0.2 6,096,794 - Pf6N2E2_5302 0.70 +0.4 6,096,794 - Pf6N2E2_5302 0.70 +1.4 6,096,794 - Pf6N2E2_5302 0.70 -0.1 6,096,794 - Pf6N2E2_5302 0.70 +0.4 6,096,794 - Pf6N2E2_5302 0.70 +1.5 6,096,794 - Pf6N2E2_5302 0.70 +1.1 6,096,845 - Pf6N2E2_5302 0.80 +0.1 6,096,943 + +0.8 6,096,943 + -0.1 6,097,213 + Pf6N2E2_5303 0.85 +0.1 6,097,419 + +1.1 6,097,420 - -0.3 6,097,420 - +0.7 6,097,429 + -0.4 6,097,429 + -0.9 6,097,429 + +0.5 6,097,429 + +0.8 6,097,430 - +0.0 6,097,430 - +1.0 6,097,430 - -0.2 6,097,529 + -0.6 6,097,573 - -0.7 6,097,637 + +1.8 6,097,638 - +0.5 6,097,651 + +0.6 6,097,651 + -0.8 6,097,651 + -0.2 6,097,651 + -0.8 6,097,651 + +0.6 6,097,652 - -0.7 6,097,700 + +0.1 6,097,744 + +0.0 6,097,745 - -0.4 6,097,753 + +1.3 6,097,765 + +0.1 6,097,765 + +0.6 6,097,765 + -0.3 6,097,766 - -0.1 6,097,766 - +0.2 6,097,795 + -0.6 6,097,796 - +2.1 6,097,796 - +0.7 6,097,831 + Pf6N2E2_5304 0.16 -0.8 6,097,831 + Pf6N2E2_5304 0.16 +1.4 6,097,831 + Pf6N2E2_5304 0.16 -0.5 6,097,831 + Pf6N2E2_5304 0.16 +0.3 6,097,831 + Pf6N2E2_5304 0.16 +0.5 6,097,831 + Pf6N2E2_5304 0.16 +1.1 6,097,831 + Pf6N2E2_5304 0.16 -0.5 6,097,831 + Pf6N2E2_5304 0.16 +0.0 6,097,831 + Pf6N2E2_5304 0.16 +0.9 6,097,832 - Pf6N2E2_5304 0.16 -0.5 6,097,832 - Pf6N2E2_5304 0.16 -0.4 6,097,832 - Pf6N2E2_5304 0.16 -1.8 6,097,832 - Pf6N2E2_5304 0.16 -1.2 6,097,832 - Pf6N2E2_5304 0.16 +0.5 6,097,832 - Pf6N2E2_5304 0.16 +0.7 6,097,832 - Pf6N2E2_5304 0.16 +1.4 6,097,832 - Pf6N2E2_5304 0.16 +0.8 6,097,832 - Pf6N2E2_5304 0.16 +0.7 6,097,832 - Pf6N2E2_5304 0.16 -0.4 6,097,834 + Pf6N2E2_5304 0.17 +0.9 6,097,834 + Pf6N2E2_5304 0.17 -0.8 6,097,835 - Pf6N2E2_5304 0.17 -1.2 6,097,835 - Pf6N2E2_5304 0.17 +0.4 6,097,835 - Pf6N2E2_5304 0.17 +1.8
Or see this region's nucleotide sequence