Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5239 and Pf6N2E2_5240 are separated by 266 nucleotides Pf6N2E2_5240 and Pf6N2E2_5241 are separated by 61 nucleotides Pf6N2E2_5241 and Pf6N2E2_5242 are separated by 707 nucleotides
Pf6N2E2_5239 - Putative sulfate permease, at 6,035,330 to 6,037,072
_5239
Pf6N2E2_5240 - FIG00953775: hypothetical protein, at 6,037,339 to 6,037,824
_5240
Pf6N2E2_5241 - Aminopeptidase N, at 6,037,886 to 6,038,173
_5241
Pf6N2E2_5242 - Malate:quinone oxidoreductase (EC 1.1.5.4), at 6,038,881 to 6,040,386
_5242
Position (kb)
6037
6038
6039 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.898 kb on - strand at 6036.898 kb on - strand at 6036.898 kb on - strand at 6036.898 kb on - strand at 6037.014 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.077 kb on - strand at 6037.077 kb on - strand at 6037.077 kb on - strand at 6037.077 kb on - strand at 6037.143 kb on - strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.155 kb on - strand at 6037.155 kb on - strand at 6037.155 kb on - strand at 6037.155 kb on - strand at 6037.158 kb on - strand at 6037.158 kb on - strand at 6037.267 kb on + strand at 6037.290 kb on - strand at 6037.307 kb on + strand at 6037.308 kb on - strand at 6037.365 kb on + strand at 6037.498 kb on + strand, within Pf6N2E2_5240 at 6037.623 kb on + strand, within Pf6N2E2_5240 at 6037.624 kb on - strand, within Pf6N2E2_5240 at 6037.696 kb on + strand, within Pf6N2E2_5240 at 6037.697 kb on - strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.751 kb on - strand, within Pf6N2E2_5240 at 6037.751 kb on - strand, within Pf6N2E2_5240 at 6037.768 kb on + strand, within Pf6N2E2_5240 at 6037.769 kb on - strand, within Pf6N2E2_5240 at 6037.769 kb on - strand, within Pf6N2E2_5240 at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.846 kb on - strand at 6038.141 kb on + strand, within Pf6N2E2_5241 at 6038.141 kb on + strand, within Pf6N2E2_5241 at 6038.225 kb on + strand at 6038.225 kb on + strand at 6038.226 kb on - strand at 6038.237 kb on + strand at 6038.237 kb on + strand at 6038.246 kb on - strand at 6038.260 kb on - strand at 6038.352 kb on + strand at 6038.375 kb on - strand at 6038.550 kb on + strand at 6038.574 kb on + strand at 6038.575 kb on - strand at 6038.579 kb on + strand at 6038.579 kb on + strand at 6038.579 kb on + strand at 6038.579 kb on + strand at 6038.579 kb on + strand at 6038.580 kb on - strand at 6038.580 kb on - strand at 6038.580 kb on - strand at 6038.580 kb on - strand at 6038.580 kb on - strand at 6038.580 kb on - strand at 6038.580 kb on - strand at 6038.582 kb on + strand at 6038.582 kb on + strand at 6038.582 kb on + strand at 6038.582 kb on + strand at 6038.582 kb on + strand at 6038.617 kb on + strand at 6038.617 kb on + strand at 6038.618 kb on - strand at 6038.618 kb on - strand at 6038.618 kb on - strand at 6038.618 kb on - strand at 6038.622 kb on - strand at 6038.622 kb on - strand at 6038.622 kb on - strand at 6038.751 kb on + strand at 6038.752 kb on - strand at 6038.754 kb on + strand at 6038.778 kb on - strand at 6038.835 kb on - strand at 6038.872 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,036,897 + Pf6N2E2_5239 0.90 +0.0 6,036,897 + Pf6N2E2_5239 0.90 +0.0 6,036,897 + Pf6N2E2_5239 0.90 -1.4 6,036,897 + Pf6N2E2_5239 0.90 +0.2 6,036,898 - +0.3 6,036,898 - -0.1 6,036,898 - +0.2 6,036,898 - -0.1 6,037,014 + +0.3 6,037,076 + +0.5 6,037,076 + +0.1 6,037,076 + -0.7 6,037,076 + -0.4 6,037,076 + +0.3 6,037,077 - -1.1 6,037,077 - +0.6 6,037,077 - -0.0 6,037,077 - -2.6 6,037,143 - -0.6 6,037,154 + +0.3 6,037,154 + -0.2 6,037,154 + +0.4 6,037,154 + +0.7 6,037,154 + +0.6 6,037,154 + -1.3 6,037,155 - +1.4 6,037,155 - -1.1 6,037,155 - +0.1 6,037,155 - +0.1 6,037,158 - -0.2 6,037,158 - +1.6 6,037,267 + -0.4 6,037,290 - +0.1 6,037,307 + -0.3 6,037,308 - +1.4 6,037,365 + +1.6 6,037,498 + Pf6N2E2_5240 0.33 +0.6 6,037,623 + Pf6N2E2_5240 0.58 +1.4 6,037,624 - Pf6N2E2_5240 0.59 -0.1 6,037,696 + Pf6N2E2_5240 0.73 +0.2 6,037,697 - Pf6N2E2_5240 0.74 -0.2 6,037,750 + Pf6N2E2_5240 0.85 +1.1 6,037,750 + Pf6N2E2_5240 0.85 -0.2 6,037,750 + Pf6N2E2_5240 0.85 +1.5 6,037,750 + Pf6N2E2_5240 0.85 +1.1 6,037,750 + Pf6N2E2_5240 0.85 +0.7 6,037,750 + Pf6N2E2_5240 0.85 +0.8 6,037,750 + Pf6N2E2_5240 0.85 +0.2 6,037,751 - Pf6N2E2_5240 0.85 +0.7 6,037,751 - Pf6N2E2_5240 0.85 +1.6 6,037,768 + Pf6N2E2_5240 0.88 +0.8 6,037,769 - Pf6N2E2_5240 0.88 -0.1 6,037,769 - Pf6N2E2_5240 0.88 -0.7 6,037,783 - +2.4 6,037,783 - +0.4 6,037,783 - +1.0 6,037,783 - +0.8 6,037,783 - +0.7 6,037,783 - +0.8 6,037,846 - -1.2 6,038,141 + Pf6N2E2_5241 0.89 -1.4 6,038,141 + Pf6N2E2_5241 0.89 -0.6 6,038,225 + +0.8 6,038,225 + +1.0 6,038,226 - -2.1 6,038,237 + -0.5 6,038,237 + +0.7 6,038,246 - +0.8 6,038,260 - +0.8 6,038,352 + -0.3 6,038,375 - -0.7 6,038,550 + -0.5 6,038,574 + +0.9 6,038,575 - +2.0 6,038,579 + -0.1 6,038,579 + -0.3 6,038,579 + +0.0 6,038,579 + -1.3 6,038,579 + +0.2 6,038,580 - +1.1 6,038,580 - -0.8 6,038,580 - -0.2 6,038,580 - +0.7 6,038,580 - -0.8 6,038,580 - +0.2 6,038,580 - +0.5 6,038,582 + +0.9 6,038,582 + +0.3 6,038,582 + +0.1 6,038,582 + +0.5 6,038,582 + +1.4 6,038,617 + -1.1 6,038,617 + -0.6 6,038,618 - +0.1 6,038,618 - +1.9 6,038,618 - +0.6 6,038,618 - +1.5 6,038,622 - +0.5 6,038,622 - -0.4 6,038,622 - +1.5 6,038,751 + -1.1 6,038,752 - +1.0 6,038,754 + +0.5 6,038,778 - +0.3 6,038,835 - -1.0 6,038,872 + +0.6
Or see this region's nucleotide sequence