Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4898 and Pf6N2E2_4899 overlap by 11 nucleotides Pf6N2E2_4899 and Pf6N2E2_4900 are separated by 129 nucleotides
Pf6N2E2_4898 - Biotin carboxylase (EC 6.3.4.14), at 5,650,818 to 5,651,921
_4898
Pf6N2E2_4899 - Prolyl oligopeptidase family protein, at 5,651,911 to 5,652,669
_4899
Pf6N2E2_4900 - Inner membrane protein YbhQ, at 5,652,799 to 5,653,800
_4900
Position (kb)
5651
5652
5653 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5650.942 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.118 kb on - strand, within Pf6N2E2_4898 at 5651.241 kb on + strand, within Pf6N2E2_4898 at 5651.241 kb on + strand, within Pf6N2E2_4898 at 5651.241 kb on + strand, within Pf6N2E2_4898 at 5651.242 kb on - strand, within Pf6N2E2_4898 at 5651.242 kb on - strand, within Pf6N2E2_4898 at 5651.448 kb on + strand, within Pf6N2E2_4898 at 5651.448 kb on + strand, within Pf6N2E2_4898 at 5651.449 kb on - strand, within Pf6N2E2_4898 at 5651.449 kb on - strand, within Pf6N2E2_4898 at 5651.449 kb on - strand, within Pf6N2E2_4898 at 5651.496 kb on + strand, within Pf6N2E2_4898 at 5651.496 kb on + strand, within Pf6N2E2_4898 at 5651.496 kb on + strand, within Pf6N2E2_4898 at 5651.497 kb on - strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.519 kb on - strand, within Pf6N2E2_4898 at 5651.521 kb on + strand, within Pf6N2E2_4898 at 5651.521 kb on + strand, within Pf6N2E2_4898 at 5651.521 kb on + strand, within Pf6N2E2_4898 at 5651.522 kb on - strand, within Pf6N2E2_4898 at 5651.522 kb on - strand, within Pf6N2E2_4898 at 5651.532 kb on + strand, within Pf6N2E2_4898 at 5651.533 kb on - strand, within Pf6N2E2_4898 at 5651.533 kb on - strand, within Pf6N2E2_4898 at 5651.534 kb on + strand, within Pf6N2E2_4898 at 5651.534 kb on + strand, within Pf6N2E2_4898 at 5651.535 kb on - strand, within Pf6N2E2_4898 at 5651.622 kb on + strand, within Pf6N2E2_4898 at 5651.653 kb on - strand, within Pf6N2E2_4898 at 5651.653 kb on - strand, within Pf6N2E2_4898 at 5651.814 kb on + strand at 5651.814 kb on + strand at 5651.815 kb on - strand at 5651.892 kb on + strand at 5651.892 kb on + strand at 5651.892 kb on + strand at 5651.892 kb on + strand at 5651.893 kb on - strand at 5651.893 kb on - strand at 5651.893 kb on - strand at 5651.893 kb on - strand at 5651.896 kb on + strand at 5651.897 kb on - strand at 5651.910 kb on + strand at 5651.910 kb on + strand at 5651.911 kb on - strand at 5651.911 kb on - strand at 5651.911 kb on - strand at 5651.911 kb on - strand at 5651.919 kb on + strand at 5651.920 kb on - strand at 5652.178 kb on + strand, within Pf6N2E2_4899 at 5652.179 kb on - strand, within Pf6N2E2_4899 at 5652.211 kb on - strand, within Pf6N2E2_4899 at 5652.241 kb on + strand, within Pf6N2E2_4899 at 5652.241 kb on + strand, within Pf6N2E2_4899 at 5652.241 kb on + strand, within Pf6N2E2_4899 at 5652.241 kb on + strand, within Pf6N2E2_4899 at 5652.241 kb on + strand, within Pf6N2E2_4899 at 5652.242 kb on - strand, within Pf6N2E2_4899 at 5652.242 kb on - strand, within Pf6N2E2_4899 at 5652.242 kb on - strand, within Pf6N2E2_4899 at 5652.242 kb on - strand, within Pf6N2E2_4899 at 5652.242 kb on - strand, within Pf6N2E2_4899 at 5652.242 kb on - strand, within Pf6N2E2_4899 at 5652.250 kb on + strand, within Pf6N2E2_4899 at 5652.316 kb on + strand, within Pf6N2E2_4899 at 5652.316 kb on + strand, within Pf6N2E2_4899 at 5652.317 kb on - strand, within Pf6N2E2_4899 at 5652.317 kb on - strand, within Pf6N2E2_4899 at 5652.317 kb on - strand, within Pf6N2E2_4899 at 5652.377 kb on - strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.475 kb on + strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.476 kb on - strand, within Pf6N2E2_4899 at 5652.596 kb on - strand at 5652.596 kb on - strand at 5652.723 kb on + strand at 5652.724 kb on - strand at 5652.735 kb on + strand at 5652.736 kb on - strand at 5652.736 kb on - strand at 5652.736 kb on - strand at 5652.736 kb on - strand at 5652.736 kb on - strand at 5652.736 kb on - strand at 5652.854 kb on + strand at 5652.854 kb on + strand at 5652.855 kb on - strand at 5652.855 kb on - strand at 5652.855 kb on - strand at 5652.890 kb on + strand at 5652.890 kb on + strand at 5652.891 kb on - strand at 5652.891 kb on - strand at 5652.891 kb on - strand at 5653.053 kb on - strand, within Pf6N2E2_4900 at 5653.337 kb on + strand, within Pf6N2E2_4900 at 5653.337 kb on + strand, within Pf6N2E2_4900 at 5653.338 kb on - strand, within Pf6N2E2_4900 at 5653.338 kb on - strand, within Pf6N2E2_4900 at 5653.413 kb on - strand, within Pf6N2E2_4900 at 5653.413 kb on - strand, within Pf6N2E2_4900 at 5653.441 kb on + strand, within Pf6N2E2_4900 at 5653.473 kb on - strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.526 kb on + strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.527 kb on - strand, within Pf6N2E2_4900 at 5653.556 kb on + strand, within Pf6N2E2_4900 at 5653.556 kb on + strand, within Pf6N2E2_4900 at 5653.556 kb on + strand, within Pf6N2E2_4900 at 5653.556 kb on + strand, within Pf6N2E2_4900 at 5653.556 kb on + strand, within Pf6N2E2_4900 at 5653.556 kb on + strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900 at 5653.557 kb on - strand, within Pf6N2E2_4900
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,650,942 - Pf6N2E2_4898 0.11 +0.2 5,650,981 - Pf6N2E2_4898 0.15 +0.2 5,650,981 - Pf6N2E2_4898 0.15 +0.9 5,650,981 - Pf6N2E2_4898 0.15 -0.5 5,650,981 - Pf6N2E2_4898 0.15 +0.1 5,651,065 - Pf6N2E2_4898 0.22 +0.8 5,651,065 - Pf6N2E2_4898 0.22 +0.2 5,651,065 - Pf6N2E2_4898 0.22 +0.2 5,651,065 - Pf6N2E2_4898 0.22 -1.1 5,651,065 - Pf6N2E2_4898 0.22 -0.3 5,651,118 - Pf6N2E2_4898 0.27 +0.1 5,651,241 + Pf6N2E2_4898 0.38 +0.2 5,651,241 + Pf6N2E2_4898 0.38 -1.0 5,651,241 + Pf6N2E2_4898 0.38 -0.7 5,651,242 - Pf6N2E2_4898 0.38 -0.2 5,651,242 - Pf6N2E2_4898 0.38 -1.6 5,651,448 + Pf6N2E2_4898 0.57 -0.8 5,651,448 + Pf6N2E2_4898 0.57 -0.0 5,651,449 - Pf6N2E2_4898 0.57 +0.5 5,651,449 - Pf6N2E2_4898 0.57 -0.4 5,651,449 - Pf6N2E2_4898 0.57 +0.5 5,651,496 + Pf6N2E2_4898 0.61 +2.1 5,651,496 + Pf6N2E2_4898 0.61 +1.1 5,651,496 + Pf6N2E2_4898 0.61 +1.0 5,651,497 - Pf6N2E2_4898 0.62 +0.1 5,651,518 + Pf6N2E2_4898 0.63 +0.2 5,651,518 + Pf6N2E2_4898 0.63 +0.4 5,651,518 + Pf6N2E2_4898 0.63 +0.3 5,651,518 + Pf6N2E2_4898 0.63 +0.5 5,651,519 - Pf6N2E2_4898 0.63 -1.9 5,651,521 + Pf6N2E2_4898 0.64 +0.6 5,651,521 + Pf6N2E2_4898 0.64 -0.4 5,651,521 + Pf6N2E2_4898 0.64 +0.9 5,651,522 - Pf6N2E2_4898 0.64 -0.8 5,651,522 - Pf6N2E2_4898 0.64 +0.7 5,651,532 + Pf6N2E2_4898 0.65 +0.2 5,651,533 - Pf6N2E2_4898 0.65 +0.8 5,651,533 - Pf6N2E2_4898 0.65 +0.1 5,651,534 + Pf6N2E2_4898 0.65 -0.3 5,651,534 + Pf6N2E2_4898 0.65 -0.3 5,651,535 - Pf6N2E2_4898 0.65 +0.4 5,651,622 + Pf6N2E2_4898 0.73 -1.5 5,651,653 - Pf6N2E2_4898 0.76 -1.2 5,651,653 - Pf6N2E2_4898 0.76 +1.2 5,651,814 + -0.1 5,651,814 + -2.0 5,651,815 - +1.0 5,651,892 + -0.2 5,651,892 + +0.1 5,651,892 + -1.0 5,651,892 + -0.2 5,651,893 - +0.7 5,651,893 - +0.1 5,651,893 - -1.1 5,651,893 - +0.1 5,651,896 + +0.7 5,651,897 - -1.9 5,651,910 + -0.0 5,651,910 + -0.5 5,651,911 - -0.6 5,651,911 - -1.2 5,651,911 - -0.7 5,651,911 - +1.3 5,651,919 + +0.5 5,651,920 - +0.1 5,652,178 + Pf6N2E2_4899 0.35 +1.8 5,652,179 - Pf6N2E2_4899 0.35 +0.3 5,652,211 - Pf6N2E2_4899 0.40 -0.1 5,652,241 + Pf6N2E2_4899 0.43 +2.4 5,652,241 + Pf6N2E2_4899 0.43 +0.6 5,652,241 + Pf6N2E2_4899 0.43 +1.3 5,652,241 + Pf6N2E2_4899 0.43 -2.5 5,652,241 + Pf6N2E2_4899 0.43 +0.5 5,652,242 - Pf6N2E2_4899 0.44 -0.2 5,652,242 - Pf6N2E2_4899 0.44 +0.3 5,652,242 - Pf6N2E2_4899 0.44 +0.5 5,652,242 - Pf6N2E2_4899 0.44 +1.0 5,652,242 - Pf6N2E2_4899 0.44 +0.1 5,652,242 - Pf6N2E2_4899 0.44 +1.4 5,652,250 + Pf6N2E2_4899 0.45 +0.2 5,652,316 + Pf6N2E2_4899 0.53 -0.1 5,652,316 + Pf6N2E2_4899 0.53 -0.1 5,652,317 - Pf6N2E2_4899 0.53 -0.7 5,652,317 - Pf6N2E2_4899 0.53 +2.3 5,652,317 - Pf6N2E2_4899 0.53 +0.7 5,652,377 - Pf6N2E2_4899 0.61 -0.8 5,652,475 + Pf6N2E2_4899 0.74 +1.1 5,652,475 + Pf6N2E2_4899 0.74 -0.1 5,652,475 + Pf6N2E2_4899 0.74 +0.4 5,652,475 + Pf6N2E2_4899 0.74 -0.2 5,652,475 + Pf6N2E2_4899 0.74 -0.2 5,652,475 + Pf6N2E2_4899 0.74 +0.2 5,652,475 + Pf6N2E2_4899 0.74 -0.0 5,652,475 + Pf6N2E2_4899 0.74 +0.4 5,652,476 - Pf6N2E2_4899 0.74 +0.5 5,652,476 - Pf6N2E2_4899 0.74 -0.9 5,652,476 - Pf6N2E2_4899 0.74 -2.0 5,652,476 - Pf6N2E2_4899 0.74 +0.8 5,652,476 - Pf6N2E2_4899 0.74 +0.2 5,652,476 - Pf6N2E2_4899 0.74 +1.0 5,652,476 - Pf6N2E2_4899 0.74 +0.0 5,652,476 - Pf6N2E2_4899 0.74 -0.0 5,652,476 - Pf6N2E2_4899 0.74 +1.5 5,652,476 - Pf6N2E2_4899 0.74 +0.7 5,652,476 - Pf6N2E2_4899 0.74 +1.3 5,652,476 - Pf6N2E2_4899 0.74 +0.9 5,652,596 - -1.0 5,652,596 - +0.1 5,652,723 + -2.3 5,652,724 - +0.2 5,652,735 + -0.4 5,652,736 - +1.0 5,652,736 - +0.4 5,652,736 - -0.2 5,652,736 - +0.1 5,652,736 - +0.2 5,652,736 - -0.5 5,652,854 + -2.1 5,652,854 + -0.0 5,652,855 - -0.3 5,652,855 - +0.2 5,652,855 - +0.9 5,652,890 + +0.1 5,652,890 + +0.0 5,652,891 - +0.3 5,652,891 - +0.5 5,652,891 - -0.2 5,653,053 - Pf6N2E2_4900 0.25 +0.9 5,653,337 + Pf6N2E2_4900 0.54 +0.2 5,653,337 + Pf6N2E2_4900 0.54 +0.4 5,653,338 - Pf6N2E2_4900 0.54 -0.1 5,653,338 - Pf6N2E2_4900 0.54 -0.9 5,653,413 - Pf6N2E2_4900 0.61 -1.2 5,653,413 - Pf6N2E2_4900 0.61 -1.3 5,653,441 + Pf6N2E2_4900 0.64 +1.0 5,653,473 - Pf6N2E2_4900 0.67 -0.5 5,653,526 + Pf6N2E2_4900 0.73 -0.2 5,653,526 + Pf6N2E2_4900 0.73 +0.5 5,653,526 + Pf6N2E2_4900 0.73 +0.2 5,653,526 + Pf6N2E2_4900 0.73 -0.0 5,653,526 + Pf6N2E2_4900 0.73 +0.2 5,653,526 + Pf6N2E2_4900 0.73 -0.3 5,653,526 + Pf6N2E2_4900 0.73 +0.0 5,653,527 - Pf6N2E2_4900 0.73 +0.1 5,653,527 - Pf6N2E2_4900 0.73 -0.3 5,653,527 - Pf6N2E2_4900 0.73 +1.0 5,653,527 - Pf6N2E2_4900 0.73 +0.0 5,653,527 - Pf6N2E2_4900 0.73 -1.8 5,653,527 - Pf6N2E2_4900 0.73 -0.7 5,653,527 - Pf6N2E2_4900 0.73 +0.5 5,653,527 - Pf6N2E2_4900 0.73 +0.6 5,653,556 + Pf6N2E2_4900 0.76 +0.0 5,653,556 + Pf6N2E2_4900 0.76 -0.3 5,653,556 + Pf6N2E2_4900 0.76 -1.3 5,653,556 + Pf6N2E2_4900 0.76 -0.1 5,653,556 + Pf6N2E2_4900 0.76 +1.1 5,653,556 + Pf6N2E2_4900 0.76 -0.1 5,653,557 - Pf6N2E2_4900 0.76 +1.3 5,653,557 - Pf6N2E2_4900 0.76 +0.5 5,653,557 - Pf6N2E2_4900 0.76 +0.2 5,653,557 - Pf6N2E2_4900 0.76 +0.2 5,653,557 - Pf6N2E2_4900 0.76 +1.0 5,653,557 - Pf6N2E2_4900 0.76 -0.3 5,653,557 - Pf6N2E2_4900 0.76 -0.0 5,653,557 - Pf6N2E2_4900 0.76 -0.3 5,653,557 - Pf6N2E2_4900 0.76 +0.9 5,653,557 - Pf6N2E2_4900 0.76 -1.4 5,653,557 - Pf6N2E2_4900 0.76 +1.1
Or see this region's nucleotide sequence