Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4222 and Pf6N2E2_4223 are separated by 323 nucleotides Pf6N2E2_4223 and Pf6N2E2_4224 overlap by 11 nucleotides Pf6N2E2_4224 and Pf6N2E2_4225 are separated by 181 nucleotides
Pf6N2E2_4222 - Esterase/lipase, at 4,912,015 to 4,912,698
_4222
Pf6N2E2_4223 - Nudix-related transcriptional regulator NrtR, at 4,913,022 to 4,913,729
_4223
Pf6N2E2_4224 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 4,913,719 to 4,914,276
_4224
Pf6N2E2_4225 - Nicotinamidase (EC 3.5.1.19), at 4,914,458 to 4,915,105
_4225
Position (kb)
4913
4914 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4912.060 kb on - strand at 4912.083 kb on - strand at 4912.132 kb on + strand, within Pf6N2E2_4222 at 4912.132 kb on + strand, within Pf6N2E2_4222 at 4912.132 kb on + strand, within Pf6N2E2_4222 at 4912.133 kb on - strand, within Pf6N2E2_4222 at 4912.258 kb on + strand, within Pf6N2E2_4222 at 4912.258 kb on + strand, within Pf6N2E2_4222 at 4912.259 kb on - strand, within Pf6N2E2_4222 at 4912.262 kb on + strand, within Pf6N2E2_4222 at 4912.262 kb on + strand, within Pf6N2E2_4222 at 4912.262 kb on + strand, within Pf6N2E2_4222 at 4912.262 kb on + strand, within Pf6N2E2_4222 at 4912.262 kb on + strand, within Pf6N2E2_4222 at 4912.262 kb on + strand, within Pf6N2E2_4222 at 4912.263 kb on - strand, within Pf6N2E2_4222 at 4912.263 kb on - strand, within Pf6N2E2_4222 at 4912.263 kb on - strand, within Pf6N2E2_4222 at 4912.286 kb on + strand, within Pf6N2E2_4222 at 4912.287 kb on - strand, within Pf6N2E2_4222 at 4912.287 kb on - strand, within Pf6N2E2_4222 at 4912.287 kb on - strand, within Pf6N2E2_4222 at 4912.316 kb on + strand, within Pf6N2E2_4222 at 4912.317 kb on - strand, within Pf6N2E2_4222 at 4912.317 kb on - strand, within Pf6N2E2_4222 at 4912.326 kb on + strand, within Pf6N2E2_4222 at 4912.326 kb on + strand, within Pf6N2E2_4222 at 4912.327 kb on - strand, within Pf6N2E2_4222 at 4912.327 kb on - strand, within Pf6N2E2_4222 at 4912.327 kb on - strand, within Pf6N2E2_4222 at 4912.608 kb on + strand, within Pf6N2E2_4222 at 4912.608 kb on + strand, within Pf6N2E2_4222 at 4912.608 kb on + strand, within Pf6N2E2_4222 at 4912.608 kb on + strand, within Pf6N2E2_4222 at 4912.608 kb on + strand, within Pf6N2E2_4222 at 4912.608 kb on + strand, within Pf6N2E2_4222 at 4912.609 kb on - strand, within Pf6N2E2_4222 at 4912.609 kb on - strand, within Pf6N2E2_4222 at 4912.609 kb on - strand, within Pf6N2E2_4222 at 4912.638 kb on + strand at 4912.639 kb on - strand at 4912.639 kb on - strand at 4912.693 kb on + strand at 4912.693 kb on + strand at 4912.693 kb on + strand at 4912.693 kb on + strand at 4912.693 kb on + strand at 4912.693 kb on + strand at 4912.693 kb on + strand at 4912.694 kb on - strand at 4912.698 kb on + strand at 4912.698 kb on + strand at 4912.698 kb on + strand at 4912.699 kb on - strand at 4912.699 kb on - strand at 4912.736 kb on + strand at 4912.736 kb on + strand at 4912.736 kb on + strand at 4912.736 kb on + strand at 4912.737 kb on - strand at 4912.737 kb on - strand at 4912.737 kb on - strand at 4912.737 kb on - strand at 4912.737 kb on - strand at 4912.783 kb on + strand at 4912.922 kb on - strand at 4912.922 kb on - strand at 4912.922 kb on - strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.929 kb on + strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.930 kb on - strand at 4912.959 kb on + strand at 4912.959 kb on + strand at 4912.959 kb on + strand at 4912.959 kb on + strand at 4912.959 kb on + strand at 4912.959 kb on + strand at 4912.959 kb on + strand at 4912.960 kb on - strand at 4912.960 kb on - strand at 4912.960 kb on - strand at 4912.960 kb on - strand at 4912.961 kb on + strand at 4912.961 kb on + strand at 4912.961 kb on + strand at 4912.961 kb on + strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.962 kb on - strand at 4912.977 kb on + strand at 4912.977 kb on + strand at 4912.977 kb on + strand at 4912.978 kb on - strand at 4912.990 kb on - strand at 4912.990 kb on - strand at 4913.023 kb on + strand at 4913.699 kb on - strand at 4913.760 kb on - strand at 4913.760 kb on - strand at 4913.760 kb on - strand at 4913.845 kb on + strand, within Pf6N2E2_4224 at 4913.845 kb on + strand, within Pf6N2E2_4224 at 4913.846 kb on - strand, within Pf6N2E2_4224 at 4913.846 kb on - strand, within Pf6N2E2_4224 at 4913.907 kb on - strand, within Pf6N2E2_4224 at 4914.005 kb on + strand, within Pf6N2E2_4224 at 4914.005 kb on + strand, within Pf6N2E2_4224 at 4914.005 kb on + strand, within Pf6N2E2_4224 at 4914.005 kb on + strand, within Pf6N2E2_4224 at 4914.006 kb on - strand, within Pf6N2E2_4224 at 4914.006 kb on - strand, within Pf6N2E2_4224 at 4914.006 kb on - strand, within Pf6N2E2_4224 at 4914.006 kb on - strand, within Pf6N2E2_4224 at 4914.006 kb on - strand, within Pf6N2E2_4224 at 4914.012 kb on - strand, within Pf6N2E2_4224 at 4914.012 kb on - strand, within Pf6N2E2_4224 at 4914.131 kb on + strand, within Pf6N2E2_4224 at 4914.132 kb on - strand, within Pf6N2E2_4224 at 4914.132 kb on - strand, within Pf6N2E2_4224 at 4914.306 kb on - strand at 4914.422 kb on - strand at 4914.422 kb on - strand at 4914.514 kb on + strand at 4914.514 kb on + strand at 4914.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,912,060 - +0.4 4,912,083 - +1.1 4,912,132 + Pf6N2E2_4222 0.17 -0.5 4,912,132 + Pf6N2E2_4222 0.17 -2.1 4,912,132 + Pf6N2E2_4222 0.17 -0.1 4,912,133 - Pf6N2E2_4222 0.17 -0.4 4,912,258 + Pf6N2E2_4222 0.36 +0.3 4,912,258 + Pf6N2E2_4222 0.36 +1.3 4,912,259 - Pf6N2E2_4222 0.36 +0.8 4,912,262 + Pf6N2E2_4222 0.36 -0.1 4,912,262 + Pf6N2E2_4222 0.36 -0.3 4,912,262 + Pf6N2E2_4222 0.36 +0.9 4,912,262 + Pf6N2E2_4222 0.36 -0.2 4,912,262 + Pf6N2E2_4222 0.36 -0.7 4,912,262 + Pf6N2E2_4222 0.36 +0.1 4,912,263 - Pf6N2E2_4222 0.36 +0.2 4,912,263 - Pf6N2E2_4222 0.36 +0.0 4,912,263 - Pf6N2E2_4222 0.36 -1.0 4,912,286 + Pf6N2E2_4222 0.40 +0.1 4,912,287 - Pf6N2E2_4222 0.40 -0.3 4,912,287 - Pf6N2E2_4222 0.40 +0.5 4,912,287 - Pf6N2E2_4222 0.40 +1.2 4,912,316 + Pf6N2E2_4222 0.44 -0.2 4,912,317 - Pf6N2E2_4222 0.44 -0.6 4,912,317 - Pf6N2E2_4222 0.44 -0.7 4,912,326 + Pf6N2E2_4222 0.45 -0.8 4,912,326 + Pf6N2E2_4222 0.45 +1.1 4,912,327 - Pf6N2E2_4222 0.46 +0.1 4,912,327 - Pf6N2E2_4222 0.46 -0.0 4,912,327 - Pf6N2E2_4222 0.46 -1.2 4,912,608 + Pf6N2E2_4222 0.87 +0.8 4,912,608 + Pf6N2E2_4222 0.87 +1.5 4,912,608 + Pf6N2E2_4222 0.87 +0.5 4,912,608 + Pf6N2E2_4222 0.87 -0.2 4,912,608 + Pf6N2E2_4222 0.87 +0.2 4,912,608 + Pf6N2E2_4222 0.87 +0.6 4,912,609 - Pf6N2E2_4222 0.87 +0.6 4,912,609 - Pf6N2E2_4222 0.87 +1.8 4,912,609 - Pf6N2E2_4222 0.87 +0.2 4,912,638 + -0.8 4,912,639 - -0.3 4,912,639 - -0.2 4,912,693 + -3.9 4,912,693 + +1.3 4,912,693 + -0.1 4,912,693 + +0.1 4,912,693 + +0.7 4,912,693 + +0.4 4,912,693 + -0.6 4,912,694 - +0.6 4,912,698 + -0.3 4,912,698 + -0.2 4,912,698 + +0.2 4,912,699 - -0.5 4,912,699 - +0.0 4,912,736 + +0.7 4,912,736 + +0.6 4,912,736 + -0.5 4,912,736 + -0.2 4,912,737 - +0.5 4,912,737 - -0.7 4,912,737 - +0.2 4,912,737 - +0.3 4,912,737 - +0.9 4,912,783 + +0.1 4,912,922 - +0.6 4,912,922 - +0.7 4,912,922 - -0.2 4,912,929 + -3.5 4,912,929 + -0.8 4,912,929 + +1.9 4,912,929 + -0.1 4,912,929 + +0.3 4,912,929 + -0.5 4,912,929 + -0.3 4,912,929 + +0.3 4,912,929 + +1.4 4,912,929 + +1.0 4,912,929 + -0.3 4,912,929 + -0.3 4,912,929 + +1.2 4,912,929 + +0.7 4,912,929 + -0.1 4,912,929 + -0.7 4,912,930 - -1.9 4,912,930 - -0.3 4,912,930 - +1.1 4,912,930 - +1.8 4,912,930 - +0.2 4,912,930 - -1.4 4,912,930 - -0.8 4,912,930 - +0.4 4,912,930 - +1.0 4,912,930 - -1.7 4,912,930 - +0.7 4,912,930 - +0.4 4,912,959 + -0.4 4,912,959 + +1.1 4,912,959 + +0.8 4,912,959 + +0.6 4,912,959 + +0.3 4,912,959 + +0.2 4,912,959 + +2.3 4,912,960 - -0.1 4,912,960 - +0.8 4,912,960 - -0.9 4,912,960 - +0.5 4,912,961 + -1.9 4,912,961 + -0.5 4,912,961 + +3.0 4,912,961 + +0.7 4,912,962 - -0.9 4,912,962 - +0.6 4,912,962 - -0.9 4,912,962 - +0.4 4,912,962 - -0.0 4,912,962 - -0.7 4,912,962 - -0.1 4,912,962 - +0.4 4,912,977 + +0.5 4,912,977 + -0.6 4,912,977 + -0.0 4,912,978 - -0.1 4,912,990 - -0.1 4,912,990 - +1.3 4,913,023 + -1.1 4,913,699 - -0.2 4,913,760 - -1.7 4,913,760 - +0.4 4,913,760 - +0.6 4,913,845 + Pf6N2E2_4224 0.23 +1.0 4,913,845 + Pf6N2E2_4224 0.23 +0.2 4,913,846 - Pf6N2E2_4224 0.23 -0.9 4,913,846 - Pf6N2E2_4224 0.23 +1.0 4,913,907 - Pf6N2E2_4224 0.34 +0.5 4,914,005 + Pf6N2E2_4224 0.51 +1.9 4,914,005 + Pf6N2E2_4224 0.51 -1.3 4,914,005 + Pf6N2E2_4224 0.51 +1.1 4,914,005 + Pf6N2E2_4224 0.51 -2.2 4,914,006 - Pf6N2E2_4224 0.51 +1.4 4,914,006 - Pf6N2E2_4224 0.51 -0.2 4,914,006 - Pf6N2E2_4224 0.51 +0.9 4,914,006 - Pf6N2E2_4224 0.51 -1.4 4,914,006 - Pf6N2E2_4224 0.51 +0.6 4,914,012 - Pf6N2E2_4224 0.53 +0.4 4,914,012 - Pf6N2E2_4224 0.53 -0.3 4,914,131 + Pf6N2E2_4224 0.74 +0.3 4,914,132 - Pf6N2E2_4224 0.74 +1.0 4,914,132 - Pf6N2E2_4224 0.74 +0.4 4,914,306 - -1.1 4,914,422 - +0.9 4,914,422 - +0.2 4,914,514 + +1.1 4,914,514 + -0.5 4,914,515 - -0.8
Or see this region's nucleotide sequence