Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4172 and Pf6N2E2_4173 overlap by 1 nucleotides Pf6N2E2_4173 and Pf6N2E2_4174 are separated by 79 nucleotides
Pf6N2E2_4172 - Methionine ABC transporter ATP-binding protein, at 4,854,059 to 4,854,928
_4172
Pf6N2E2_4173 - Methionine ABC transporter permease protein, at 4,854,928 to 4,855,602
_4173
Pf6N2E2_4174 - Methionine ABC transporter substrate-binding protein, at 4,855,682 to 4,856,455
_4174
Position (kb)
4855
4856
4857 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4854.744 kb on - strand, within Pf6N2E2_4172 at 4854.744 kb on - strand, within Pf6N2E2_4172 at 4854.744 kb on - strand, within Pf6N2E2_4172 at 4854.788 kb on + strand, within Pf6N2E2_4172 at 4854.788 kb on + strand, within Pf6N2E2_4172 at 4854.788 kb on + strand, within Pf6N2E2_4172 at 4854.789 kb on - strand, within Pf6N2E2_4172 at 4854.789 kb on - strand, within Pf6N2E2_4172 at 4854.789 kb on - strand, within Pf6N2E2_4172 at 4854.789 kb on - strand, within Pf6N2E2_4172 at 4855.061 kb on + strand, within Pf6N2E2_4173 at 4855.061 kb on + strand, within Pf6N2E2_4173 at 4855.062 kb on - strand, within Pf6N2E2_4173 at 4855.062 kb on - strand, within Pf6N2E2_4173 at 4855.105 kb on + strand, within Pf6N2E2_4173 at 4855.105 kb on + strand, within Pf6N2E2_4173 at 4855.105 kb on + strand, within Pf6N2E2_4173 at 4855.105 kb on + strand, within Pf6N2E2_4173 at 4855.105 kb on + strand, within Pf6N2E2_4173 at 4855.106 kb on - strand, within Pf6N2E2_4173 at 4855.106 kb on - strand, within Pf6N2E2_4173 at 4855.106 kb on - strand, within Pf6N2E2_4173 at 4855.398 kb on + strand, within Pf6N2E2_4173 at 4855.426 kb on + strand, within Pf6N2E2_4173 at 4855.583 kb on + strand at 4855.601 kb on - strand at 4855.675 kb on + strand at 4855.676 kb on - strand at 4855.676 kb on - strand at 4855.676 kb on - strand at 4855.676 kb on - strand at 4855.692 kb on + strand at 4855.693 kb on - strand at 4855.693 kb on - strand at 4855.693 kb on - strand at 4855.693 kb on - strand at 4855.850 kb on + strand, within Pf6N2E2_4174 at 4855.850 kb on + strand, within Pf6N2E2_4174 at 4855.850 kb on + strand, within Pf6N2E2_4174 at 4855.851 kb on - strand, within Pf6N2E2_4174 at 4855.851 kb on - strand, within Pf6N2E2_4174 at 4855.866 kb on + strand, within Pf6N2E2_4174 at 4855.867 kb on - strand, within Pf6N2E2_4174 at 4855.867 kb on - strand, within Pf6N2E2_4174 at 4855.867 kb on - strand, within Pf6N2E2_4174 at 4855.867 kb on - strand, within Pf6N2E2_4174 at 4855.916 kb on + strand, within Pf6N2E2_4174 at 4855.917 kb on - strand, within Pf6N2E2_4174 at 4855.917 kb on - strand, within Pf6N2E2_4174 at 4855.953 kb on + strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.954 kb on - strand, within Pf6N2E2_4174 at 4855.995 kb on - strand, within Pf6N2E2_4174 at 4856.006 kb on + strand, within Pf6N2E2_4174 at 4856.006 kb on + strand, within Pf6N2E2_4174 at 4856.006 kb on + strand, within Pf6N2E2_4174 at 4856.006 kb on + strand, within Pf6N2E2_4174 at 4856.007 kb on - strand, within Pf6N2E2_4174 at 4856.007 kb on - strand, within Pf6N2E2_4174 at 4856.007 kb on - strand, within Pf6N2E2_4174 at 4856.007 kb on - strand, within Pf6N2E2_4174 at 4856.018 kb on - strand, within Pf6N2E2_4174 at 4856.018 kb on - strand, within Pf6N2E2_4174 at 4856.018 kb on - strand, within Pf6N2E2_4174 at 4856.018 kb on - strand, within Pf6N2E2_4174 at 4856.018 kb on - strand, within Pf6N2E2_4174 at 4856.245 kb on + strand, within Pf6N2E2_4174 at 4856.246 kb on - strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.252 kb on + strand, within Pf6N2E2_4174 at 4856.253 kb on - strand, within Pf6N2E2_4174 at 4856.253 kb on - strand, within Pf6N2E2_4174 at 4856.253 kb on - strand, within Pf6N2E2_4174 at 4856.305 kb on + strand, within Pf6N2E2_4174 at 4856.305 kb on + strand, within Pf6N2E2_4174 at 4856.318 kb on + strand, within Pf6N2E2_4174 at 4856.318 kb on + strand, within Pf6N2E2_4174 at 4856.318 kb on + strand, within Pf6N2E2_4174 at 4856.318 kb on + strand, within Pf6N2E2_4174 at 4856.319 kb on - strand, within Pf6N2E2_4174 at 4856.319 kb on - strand, within Pf6N2E2_4174 at 4856.413 kb on + strand at 4856.414 kb on - strand at 4856.426 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.462 kb on + strand at 4856.463 kb on - strand at 4856.463 kb on - strand at 4856.463 kb on - strand at 4856.463 kb on - strand at 4856.463 kb on - strand at 4856.488 kb on - strand at 4856.523 kb on - strand at 4856.579 kb on + strand at 4856.579 kb on + strand at 4856.580 kb on - strand at 4856.580 kb on - strand at 4856.580 kb on - strand at 4856.592 kb on - strand at 4856.595 kb on + strand at 4856.596 kb on - strand at 4856.596 kb on - strand at 4856.614 kb on + strand at 4856.614 kb on + strand at 4856.614 kb on + strand at 4856.615 kb on - strand at 4856.620 kb on + strand at 4856.621 kb on - strand at 4856.621 kb on - strand at 4856.689 kb on + strand at 4856.690 kb on - strand at 4856.804 kb on + strand at 4856.808 kb on + strand at 4856.808 kb on + strand at 4856.809 kb on - strand at 4856.874 kb on + strand at 4856.874 kb on + strand at 4856.874 kb on + strand at 4856.874 kb on + strand at 4856.875 kb on - strand at 4856.875 kb on - strand at 4856.960 kb on + strand at 4856.960 kb on + strand at 4856.961 kb on - strand at 4856.965 kb on + strand at 4856.965 kb on + strand at 4856.965 kb on + strand at 4856.965 kb on + strand at 4856.965 kb on + strand at 4856.966 kb on - strand at 4856.966 kb on - strand at 4856.966 kb on - strand at 4856.966 kb on - strand at 4856.969 kb on + strand at 4856.969 kb on + strand at 4856.970 kb on - strand at 4856.970 kb on - strand at 4856.970 kb on - strand at 4856.970 kb on - strand at 4857.035 kb on + strand at 4857.036 kb on - strand at 4857.036 kb on - strand at 4857.090 kb on - strand at 4857.114 kb on - strand at 4857.114 kb on - strand at 4857.114 kb on - strand at 4857.118 kb on - strand at 4857.215 kb on + strand at 4857.216 kb on - strand at 4857.216 kb on - strand at 4857.216 kb on - strand at 4857.329 kb on + strand at 4857.340 kb on + strand at 4857.340 kb on + strand at 4857.359 kb on - strand at 4857.384 kb on + strand at 4857.385 kb on - strand at 4857.385 kb on - strand at 4857.405 kb on + strand at 4857.406 kb on - strand at 4857.406 kb on - strand at 4857.406 kb on - strand at 4857.453 kb on + strand at 4857.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,854,744 - Pf6N2E2_4172 0.79 -0.0 4,854,744 - Pf6N2E2_4172 0.79 +0.2 4,854,744 - Pf6N2E2_4172 0.79 +0.3 4,854,788 + Pf6N2E2_4172 0.84 +2.2 4,854,788 + Pf6N2E2_4172 0.84 +1.0 4,854,788 + Pf6N2E2_4172 0.84 +1.3 4,854,789 - Pf6N2E2_4172 0.84 +0.0 4,854,789 - Pf6N2E2_4172 0.84 -0.1 4,854,789 - Pf6N2E2_4172 0.84 +0.7 4,854,789 - Pf6N2E2_4172 0.84 -0.3 4,855,061 + Pf6N2E2_4173 0.20 +1.3 4,855,061 + Pf6N2E2_4173 0.20 -0.2 4,855,062 - Pf6N2E2_4173 0.20 +0.7 4,855,062 - Pf6N2E2_4173 0.20 -0.5 4,855,105 + Pf6N2E2_4173 0.26 -1.2 4,855,105 + Pf6N2E2_4173 0.26 +0.5 4,855,105 + Pf6N2E2_4173 0.26 +0.5 4,855,105 + Pf6N2E2_4173 0.26 +0.9 4,855,105 + Pf6N2E2_4173 0.26 +0.9 4,855,106 - Pf6N2E2_4173 0.26 -2.0 4,855,106 - Pf6N2E2_4173 0.26 -3.0 4,855,106 - Pf6N2E2_4173 0.26 +0.1 4,855,398 + Pf6N2E2_4173 0.70 -1.2 4,855,426 + Pf6N2E2_4173 0.74 +2.4 4,855,583 + +0.7 4,855,601 - +0.5 4,855,675 + -0.3 4,855,676 - -1.2 4,855,676 - +0.6 4,855,676 - -0.1 4,855,676 - -1.3 4,855,692 + +0.0 4,855,693 - +0.3 4,855,693 - +0.1 4,855,693 - -0.4 4,855,693 - -0.1 4,855,850 + Pf6N2E2_4174 0.22 -0.2 4,855,850 + Pf6N2E2_4174 0.22 -0.6 4,855,850 + Pf6N2E2_4174 0.22 +0.1 4,855,851 - Pf6N2E2_4174 0.22 -0.8 4,855,851 - Pf6N2E2_4174 0.22 -0.5 4,855,866 + Pf6N2E2_4174 0.24 -1.9 4,855,867 - Pf6N2E2_4174 0.24 -0.2 4,855,867 - Pf6N2E2_4174 0.24 -0.4 4,855,867 - Pf6N2E2_4174 0.24 -0.9 4,855,867 - Pf6N2E2_4174 0.24 -1.2 4,855,916 + Pf6N2E2_4174 0.30 +0.2 4,855,917 - Pf6N2E2_4174 0.30 -1.9 4,855,917 - Pf6N2E2_4174 0.30 -1.7 4,855,953 + Pf6N2E2_4174 0.35 -0.2 4,855,954 - Pf6N2E2_4174 0.35 -0.2 4,855,954 - Pf6N2E2_4174 0.35 -1.0 4,855,954 - Pf6N2E2_4174 0.35 -0.9 4,855,954 - Pf6N2E2_4174 0.35 -3.3 4,855,954 - Pf6N2E2_4174 0.35 +1.0 4,855,954 - Pf6N2E2_4174 0.35 +1.8 4,855,954 - Pf6N2E2_4174 0.35 -0.1 4,855,995 - Pf6N2E2_4174 0.40 +0.6 4,856,006 + Pf6N2E2_4174 0.42 -0.6 4,856,006 + Pf6N2E2_4174 0.42 -0.8 4,856,006 + Pf6N2E2_4174 0.42 -1.0 4,856,006 + Pf6N2E2_4174 0.42 -0.5 4,856,007 - Pf6N2E2_4174 0.42 +0.0 4,856,007 - Pf6N2E2_4174 0.42 -0.1 4,856,007 - Pf6N2E2_4174 0.42 +0.5 4,856,007 - Pf6N2E2_4174 0.42 -0.4 4,856,018 - Pf6N2E2_4174 0.43 -0.8 4,856,018 - Pf6N2E2_4174 0.43 -0.5 4,856,018 - Pf6N2E2_4174 0.43 -0.6 4,856,018 - Pf6N2E2_4174 0.43 -0.6 4,856,018 - Pf6N2E2_4174 0.43 -0.4 4,856,245 + Pf6N2E2_4174 0.73 -1.7 4,856,246 - Pf6N2E2_4174 0.73 -1.1 4,856,252 + Pf6N2E2_4174 0.74 -2.1 4,856,252 + Pf6N2E2_4174 0.74 -0.1 4,856,252 + Pf6N2E2_4174 0.74 +0.3 4,856,252 + Pf6N2E2_4174 0.74 +0.1 4,856,252 + Pf6N2E2_4174 0.74 -0.8 4,856,252 + Pf6N2E2_4174 0.74 -0.3 4,856,252 + Pf6N2E2_4174 0.74 +1.5 4,856,252 + Pf6N2E2_4174 0.74 -2.1 4,856,253 - Pf6N2E2_4174 0.74 +0.5 4,856,253 - Pf6N2E2_4174 0.74 +1.2 4,856,253 - Pf6N2E2_4174 0.74 -0.2 4,856,305 + Pf6N2E2_4174 0.80 -1.1 4,856,305 + Pf6N2E2_4174 0.80 -0.7 4,856,318 + Pf6N2E2_4174 0.82 -0.4 4,856,318 + Pf6N2E2_4174 0.82 -1.1 4,856,318 + Pf6N2E2_4174 0.82 +0.2 4,856,318 + Pf6N2E2_4174 0.82 -0.3 4,856,319 - Pf6N2E2_4174 0.82 +0.3 4,856,319 - Pf6N2E2_4174 0.82 +0.1 4,856,413 + -1.3 4,856,414 - +0.4 4,856,426 + -0.1 4,856,462 + -0.3 4,856,462 + -0.4 4,856,462 + -0.1 4,856,462 + +0.0 4,856,462 + -0.2 4,856,462 + +0.1 4,856,462 + -0.1 4,856,462 + +0.4 4,856,462 + -0.2 4,856,462 + -0.9 4,856,463 - -0.1 4,856,463 - -0.6 4,856,463 - +0.5 4,856,463 - -1.5 4,856,463 - -0.4 4,856,488 - -0.9 4,856,523 - -1.5 4,856,579 + +0.6 4,856,579 + -0.1 4,856,580 - +0.8 4,856,580 - -0.2 4,856,580 - +0.3 4,856,592 - +0.2 4,856,595 + -0.8 4,856,596 - -2.3 4,856,596 - -0.3 4,856,614 + +1.5 4,856,614 + +0.0 4,856,614 + +0.7 4,856,615 - +0.7 4,856,620 + +0.9 4,856,621 - -0.0 4,856,621 - +1.2 4,856,689 + +0.4 4,856,690 - +0.4 4,856,804 + +0.3 4,856,808 + +2.1 4,856,808 + +0.4 4,856,809 - -1.1 4,856,874 + +1.1 4,856,874 + -1.4 4,856,874 + +1.0 4,856,874 + -0.6 4,856,875 - +1.1 4,856,875 - +0.3 4,856,960 + +1.1 4,856,960 + +0.7 4,856,961 - -0.3 4,856,965 + +1.1 4,856,965 + +0.3 4,856,965 + -0.2 4,856,965 + -1.7 4,856,965 + -1.1 4,856,966 - +0.8 4,856,966 - -1.1 4,856,966 - -0.4 4,856,966 - +0.3 4,856,969 + -0.1 4,856,969 + -1.2 4,856,970 - +1.1 4,856,970 - +0.1 4,856,970 - +0.3 4,856,970 - +0.5 4,857,035 + +0.8 4,857,036 - +1.3 4,857,036 - +0.6 4,857,090 - -0.3 4,857,114 - -0.1 4,857,114 - +0.6 4,857,114 - +0.8 4,857,118 - +0.4 4,857,215 + +0.2 4,857,216 - +0.2 4,857,216 - -0.0 4,857,216 - -0.4 4,857,329 + -0.4 4,857,340 + -1.0 4,857,340 + +0.1 4,857,359 - -0.8 4,857,384 + -0.7 4,857,385 - +1.0 4,857,385 - +1.2 4,857,405 + +1.6 4,857,406 - -0.9 4,857,406 - +2.2 4,857,406 - +0.4 4,857,453 + +0.2 4,857,454 - -0.8
Or see this region's nucleotide sequence