Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3449 and Pf6N2E2_3450 are separated by 97 nucleotides Pf6N2E2_3450 and Pf6N2E2_3451 are separated by 133 nucleotides Pf6N2E2_3451 and Pf6N2E2_3452 are separated by 10 nucleotides
Pf6N2E2_3449 - Hydrogen peroxide-inducible genes activator, at 4,017,582 to 4,018,463
_3449
Pf6N2E2_3450 - protein, at 4,018,561 to 4,018,905
_3450
Pf6N2E2_3451 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 4,019,039 to 4,019,926
_3451
Pf6N2E2_3452 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27), at 4,019,937 to 4,021,463
_3452
Position (kb)
4019
4020 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4018.085 kb on - strand, within Pf6N2E2_3449 at 4018.129 kb on + strand, within Pf6N2E2_3449 at 4018.158 kb on + strand, within Pf6N2E2_3449 at 4018.158 kb on + strand, within Pf6N2E2_3449 at 4018.159 kb on - strand, within Pf6N2E2_3449 at 4018.159 kb on - strand, within Pf6N2E2_3449 at 4018.159 kb on - strand, within Pf6N2E2_3449 at 4018.159 kb on - strand, within Pf6N2E2_3449 at 4018.159 kb on - strand, within Pf6N2E2_3449 at 4018.159 kb on - strand, within Pf6N2E2_3449 at 4018.229 kb on + strand, within Pf6N2E2_3449 at 4018.229 kb on + strand, within Pf6N2E2_3449 at 4018.229 kb on + strand, within Pf6N2E2_3449 at 4018.229 kb on + strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.230 kb on - strand, within Pf6N2E2_3449 at 4018.274 kb on + strand, within Pf6N2E2_3449 at 4018.274 kb on + strand, within Pf6N2E2_3449 at 4018.275 kb on - strand, within Pf6N2E2_3449 at 4018.275 kb on - strand, within Pf6N2E2_3449 at 4018.275 kb on - strand, within Pf6N2E2_3449 at 4018.347 kb on + strand, within Pf6N2E2_3449 at 4018.347 kb on + strand, within Pf6N2E2_3449 at 4018.347 kb on + strand, within Pf6N2E2_3449 at 4018.348 kb on - strand, within Pf6N2E2_3449 at 4018.348 kb on - strand, within Pf6N2E2_3449 at 4018.503 kb on + strand at 4018.503 kb on + strand at 4018.503 kb on + strand at 4018.669 kb on + strand, within Pf6N2E2_3450 at 4018.669 kb on + strand, within Pf6N2E2_3450 at 4018.670 kb on - strand, within Pf6N2E2_3450 at 4018.670 kb on - strand, within Pf6N2E2_3450 at 4018.729 kb on + strand, within Pf6N2E2_3450 at 4018.729 kb on + strand, within Pf6N2E2_3450 at 4018.730 kb on - strand, within Pf6N2E2_3450 at 4018.730 kb on - strand, within Pf6N2E2_3450 at 4018.739 kb on - strand, within Pf6N2E2_3450 at 4018.754 kb on - strand, within Pf6N2E2_3450 at 4018.754 kb on - strand, within Pf6N2E2_3450 at 4018.774 kb on + strand, within Pf6N2E2_3450 at 4018.888 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.059 kb on + strand at 4019.060 kb on - strand at 4019.060 kb on - strand at 4019.060 kb on - strand at 4019.060 kb on - strand at 4019.060 kb on - strand at 4019.060 kb on - strand at 4019.062 kb on + strand at 4019.062 kb on + strand at 4019.062 kb on + strand at 4019.063 kb on - strand at 4019.063 kb on - strand at 4019.115 kb on + strand at 4019.115 kb on + strand at 4019.115 kb on + strand at 4019.115 kb on + strand at 4019.116 kb on - strand at 4019.116 kb on - strand at 4019.225 kb on - strand, within Pf6N2E2_3451 at 4019.225 kb on - strand, within Pf6N2E2_3451 at 4019.225 kb on - strand, within Pf6N2E2_3451 at 4019.274 kb on + strand, within Pf6N2E2_3451 at 4019.274 kb on + strand, within Pf6N2E2_3451 at 4019.274 kb on + strand, within Pf6N2E2_3451 at 4019.274 kb on + strand, within Pf6N2E2_3451 at 4019.274 kb on + strand, within Pf6N2E2_3451 at 4019.274 kb on + strand, within Pf6N2E2_3451 at 4019.275 kb on - strand, within Pf6N2E2_3451 at 4019.275 kb on - strand, within Pf6N2E2_3451 at 4019.275 kb on - strand, within Pf6N2E2_3451 at 4019.275 kb on - strand, within Pf6N2E2_3451 at 4019.537 kb on - strand, within Pf6N2E2_3451 at 4019.678 kb on - strand, within Pf6N2E2_3451 at 4019.911 kb on + strand at 4019.911 kb on + strand at 4019.912 kb on - strand at 4019.912 kb on - strand at 4020.022 kb on + strand at 4020.022 kb on + strand at 4020.047 kb on - strand at 4020.299 kb on - strand, within Pf6N2E2_3452 at 4020.299 kb on - strand, within Pf6N2E2_3452 at 4020.299 kb on - strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.731 kb on - strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,018,085 - Pf6N2E2_3449 0.57 -0.4 4,018,129 + Pf6N2E2_3449 0.62 +0.3 4,018,158 + Pf6N2E2_3449 0.65 +0.7 4,018,158 + Pf6N2E2_3449 0.65 +0.3 4,018,159 - Pf6N2E2_3449 0.65 -2.4 4,018,159 - Pf6N2E2_3449 0.65 +0.7 4,018,159 - Pf6N2E2_3449 0.65 -1.5 4,018,159 - Pf6N2E2_3449 0.65 +0.4 4,018,159 - Pf6N2E2_3449 0.65 +1.4 4,018,159 - Pf6N2E2_3449 0.65 +0.4 4,018,229 + Pf6N2E2_3449 0.73 +1.0 4,018,229 + Pf6N2E2_3449 0.73 +0.7 4,018,229 + Pf6N2E2_3449 0.73 +0.9 4,018,229 + Pf6N2E2_3449 0.73 +1.4 4,018,230 - Pf6N2E2_3449 0.73 -0.4 4,018,230 - Pf6N2E2_3449 0.73 -0.1 4,018,230 - Pf6N2E2_3449 0.73 -0.1 4,018,230 - Pf6N2E2_3449 0.73 +1.2 4,018,230 - Pf6N2E2_3449 0.73 +0.6 4,018,230 - Pf6N2E2_3449 0.73 -0.1 4,018,230 - Pf6N2E2_3449 0.73 -0.1 4,018,230 - Pf6N2E2_3449 0.73 +0.7 4,018,230 - Pf6N2E2_3449 0.73 -0.6 4,018,274 + Pf6N2E2_3449 0.78 -0.0 4,018,274 + Pf6N2E2_3449 0.78 +1.5 4,018,275 - Pf6N2E2_3449 0.79 +1.6 4,018,275 - Pf6N2E2_3449 0.79 +0.2 4,018,275 - Pf6N2E2_3449 0.79 +0.9 4,018,347 + Pf6N2E2_3449 0.87 +0.3 4,018,347 + Pf6N2E2_3449 0.87 +0.6 4,018,347 + Pf6N2E2_3449 0.87 -1.3 4,018,348 - Pf6N2E2_3449 0.87 -0.4 4,018,348 - Pf6N2E2_3449 0.87 -3.6 4,018,503 + -0.8 4,018,503 + +1.8 4,018,503 + -0.4 4,018,669 + Pf6N2E2_3450 0.31 +0.3 4,018,669 + Pf6N2E2_3450 0.31 -0.3 4,018,670 - Pf6N2E2_3450 0.32 +1.6 4,018,670 - Pf6N2E2_3450 0.32 -0.3 4,018,729 + Pf6N2E2_3450 0.49 +0.3 4,018,729 + Pf6N2E2_3450 0.49 -0.5 4,018,730 - Pf6N2E2_3450 0.49 +0.2 4,018,730 - Pf6N2E2_3450 0.49 -0.0 4,018,739 - Pf6N2E2_3450 0.52 +0.7 4,018,754 - Pf6N2E2_3450 0.56 -0.7 4,018,754 - Pf6N2E2_3450 0.56 +1.4 4,018,774 + Pf6N2E2_3450 0.62 -1.3 4,018,888 + +1.2 4,019,059 + -0.1 4,019,059 + +0.8 4,019,059 + -0.4 4,019,059 + +0.2 4,019,059 + +0.2 4,019,059 + -0.5 4,019,059 + -2.2 4,019,059 + +1.0 4,019,059 + +0.6 4,019,059 + +0.3 4,019,059 + -0.9 4,019,060 - -0.3 4,019,060 - -1.1 4,019,060 - +0.1 4,019,060 - -0.8 4,019,060 - +0.8 4,019,060 - -0.3 4,019,062 + +0.0 4,019,062 + +0.6 4,019,062 + +1.4 4,019,063 - -2.4 4,019,063 - +0.0 4,019,115 + +1.2 4,019,115 + +0.6 4,019,115 + -0.7 4,019,115 + -1.4 4,019,116 - -0.3 4,019,116 - -0.0 4,019,225 - Pf6N2E2_3451 0.21 -1.9 4,019,225 - Pf6N2E2_3451 0.21 +1.0 4,019,225 - Pf6N2E2_3451 0.21 -0.3 4,019,274 + Pf6N2E2_3451 0.26 +0.9 4,019,274 + Pf6N2E2_3451 0.26 -0.8 4,019,274 + Pf6N2E2_3451 0.26 -1.7 4,019,274 + Pf6N2E2_3451 0.26 +0.1 4,019,274 + Pf6N2E2_3451 0.26 +0.3 4,019,274 + Pf6N2E2_3451 0.26 -3.0 4,019,275 - Pf6N2E2_3451 0.27 +0.8 4,019,275 - Pf6N2E2_3451 0.27 +0.7 4,019,275 - Pf6N2E2_3451 0.27 -0.0 4,019,275 - Pf6N2E2_3451 0.27 -1.0 4,019,537 - Pf6N2E2_3451 0.56 -1.3 4,019,678 - Pf6N2E2_3451 0.72 +1.0 4,019,911 + +0.0 4,019,911 + -1.4 4,019,912 - -1.7 4,019,912 - +1.0 4,020,022 + +1.0 4,020,022 + +0.6 4,020,047 - -2.9 4,020,299 - Pf6N2E2_3452 0.24 -0.1 4,020,299 - Pf6N2E2_3452 0.24 +1.1 4,020,299 - Pf6N2E2_3452 0.24 +0.7 4,020,730 + Pf6N2E2_3452 0.52 +0.4 4,020,730 + Pf6N2E2_3452 0.52 +0.3 4,020,730 + Pf6N2E2_3452 0.52 +0.7 4,020,730 + Pf6N2E2_3452 0.52 -0.7 4,020,730 + Pf6N2E2_3452 0.52 -0.5 4,020,730 + Pf6N2E2_3452 0.52 +0.1 4,020,730 + Pf6N2E2_3452 0.52 +1.1 4,020,731 - Pf6N2E2_3452 0.52 +1.2 4,020,926 + Pf6N2E2_3452 0.65 -0.4 4,020,926 + Pf6N2E2_3452 0.65 +0.4 4,020,926 + Pf6N2E2_3452 0.65 +0.9 4,020,926 + Pf6N2E2_3452 0.65 -1.4
Or see this region's nucleotide sequence