Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2922 and Pf6N2E2_2923 overlap by 18 nucleotides Pf6N2E2_2923 and Pf6N2E2_2924 overlap by 4 nucleotides Pf6N2E2_2924 and Pf6N2E2_2925 overlap by 4 nucleotides Pf6N2E2_2925 and Pf6N2E2_2926 are separated by 0 nucleotides
Pf6N2E2_2922 - hypothetical protein, at 3,415,998 to 3,416,270
_2922
Pf6N2E2_2923 - ABC transporter for L-leucine/L-phenylalanine/D-alanine, permease component 1 LivH (from data) , at 3,416,253 to 3,417,176
_2923
Pf6N2E2_2924 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, permease component 2 LivM (from data) , at 3,417,173 to 3,418,429
_2924
Pf6N2E2_2925 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 1 LivG (from data) , at 3,418,426 to 3,419,193
_2925
Pf6N2E2_2926 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data) , at 3,419,194 to 3,419,895
_2926
Position (kb)
3417
3418
3419 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3416.229 kb on + strand, within Pf6N2E2_2922 at 3416.230 kb on - strand, within Pf6N2E2_2922 at 3416.265 kb on + strand at 3416.265 kb on + strand at 3416.266 kb on - strand at 3416.266 kb on - strand at 3416.266 kb on - strand at 3416.266 kb on - strand at 3416.313 kb on + strand at 3416.313 kb on + strand at 3416.313 kb on + strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.314 kb on - strand at 3416.334 kb on + strand at 3416.335 kb on - strand at 3416.336 kb on + strand at 3416.337 kb on - strand at 3416.337 kb on - strand at 3416.337 kb on - strand at 3416.337 kb on - strand at 3416.337 kb on - strand at 3416.347 kb on - strand, within Pf6N2E2_2923 at 3416.347 kb on - strand, within Pf6N2E2_2923 at 3416.388 kb on + strand, within Pf6N2E2_2923 at 3416.388 kb on + strand, within Pf6N2E2_2923 at 3416.388 kb on + strand, within Pf6N2E2_2923 at 3416.388 kb on + strand, within Pf6N2E2_2923 at 3416.388 kb on + strand, within Pf6N2E2_2923 at 3416.388 kb on + strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.389 kb on - strand, within Pf6N2E2_2923 at 3416.403 kb on + strand, within Pf6N2E2_2923 at 3416.404 kb on - strand, within Pf6N2E2_2923 at 3416.404 kb on - strand, within Pf6N2E2_2923 at 3416.404 kb on - strand, within Pf6N2E2_2923 at 3416.404 kb on - strand, within Pf6N2E2_2923 at 3416.404 kb on - strand, within Pf6N2E2_2923 at 3416.508 kb on + strand, within Pf6N2E2_2923 at 3416.712 kb on + strand, within Pf6N2E2_2923 at 3416.712 kb on + strand, within Pf6N2E2_2923 at 3416.712 kb on + strand, within Pf6N2E2_2923 at 3416.928 kb on + strand, within Pf6N2E2_2923 at 3416.929 kb on - strand, within Pf6N2E2_2923 at 3417.084 kb on + strand at 3417.084 kb on + strand at 3417.084 kb on + strand at 3417.085 kb on - strand at 3417.085 kb on - strand at 3417.172 kb on + strand at 3417.172 kb on + strand at 3417.172 kb on + strand at 3417.173 kb on - strand at 3417.173 kb on - strand at 3417.173 kb on - strand at 3417.178 kb on + strand at 3417.179 kb on - strand at 3417.179 kb on - strand at 3417.179 kb on - strand at 3417.179 kb on - strand at 3417.179 kb on - strand at 3417.247 kb on + strand at 3417.248 kb on - strand at 3417.248 kb on - strand at 3417.265 kb on + strand at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.540 kb on - strand, within Pf6N2E2_2924 at 3417.540 kb on - strand, within Pf6N2E2_2924 at 3417.540 kb on - strand, within Pf6N2E2_2924 at 3417.574 kb on - strand, within Pf6N2E2_2924 at 3417.614 kb on + strand, within Pf6N2E2_2924 at 3417.614 kb on + strand, within Pf6N2E2_2924 at 3417.614 kb on + strand, within Pf6N2E2_2924 at 3417.630 kb on - strand, within Pf6N2E2_2924 at 3417.630 kb on - strand, within Pf6N2E2_2924 at 3417.635 kb on + strand, within Pf6N2E2_2924 at 3417.635 kb on + strand, within Pf6N2E2_2924 at 3417.635 kb on + strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.653 kb on + strand, within Pf6N2E2_2924 at 3417.653 kb on + strand, within Pf6N2E2_2924 at 3417.654 kb on - strand, within Pf6N2E2_2924 at 3417.654 kb on - strand, within Pf6N2E2_2924 at 3417.756 kb on - strand, within Pf6N2E2_2924 at 3417.756 kb on - strand, within Pf6N2E2_2924 at 3417.768 kb on + strand, within Pf6N2E2_2924 at 3417.768 kb on + strand, within Pf6N2E2_2924 at 3417.890 kb on - strand, within Pf6N2E2_2924 at 3417.924 kb on - strand, within Pf6N2E2_2924 at 3417.924 kb on - strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.939 kb on - strand, within Pf6N2E2_2924 at 3417.939 kb on - strand, within Pf6N2E2_2924 at 3417.939 kb on - strand, within Pf6N2E2_2924 at 3417.969 kb on - strand, within Pf6N2E2_2924 at 3417.969 kb on - strand, within Pf6N2E2_2924 at 3417.969 kb on - strand, within Pf6N2E2_2924 at 3418.397 kb on - strand at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.641 kb on - strand, within Pf6N2E2_2925 at 3418.673 kb on + strand, within Pf6N2E2_2925 at 3418.822 kb on + strand, within Pf6N2E2_2925 at 3419.191 kb on + strand at 3419.191 kb on + strand at 3419.191 kb on + strand at 3419.191 kb on + strand at 3419.192 kb on - strand at 3419.225 kb on - strand at 3419.371 kb on + strand, within Pf6N2E2_2926 at 3419.371 kb on + strand, within Pf6N2E2_2926
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,416,229 + Pf6N2E2_2922 0.85 -2.7 3,416,230 - Pf6N2E2_2922 0.85 +2.2 3,416,265 + -1.0 3,416,265 + +2.0 3,416,266 - +1.7 3,416,266 - +1.0 3,416,266 - +1.7 3,416,266 - +2.6 3,416,313 + +2.7 3,416,313 + +3.1 3,416,313 + +1.3 3,416,314 - +1.3 3,416,314 - +1.3 3,416,314 - +1.6 3,416,314 - +1.3 3,416,314 - +0.8 3,416,314 - +0.9 3,416,314 - +1.6 3,416,314 - +2.1 3,416,334 + +3.0 3,416,335 - +1.7 3,416,336 + +0.7 3,416,337 - +2.1 3,416,337 - +2.4 3,416,337 - +1.7 3,416,337 - +1.8 3,416,337 - +2.0 3,416,347 - Pf6N2E2_2923 0.10 +2.3 3,416,347 - Pf6N2E2_2923 0.10 +2.4 3,416,388 + Pf6N2E2_2923 0.15 +1.8 3,416,388 + Pf6N2E2_2923 0.15 +2.7 3,416,388 + Pf6N2E2_2923 0.15 -0.2 3,416,388 + Pf6N2E2_2923 0.15 +1.5 3,416,388 + Pf6N2E2_2923 0.15 +0.8 3,416,388 + Pf6N2E2_2923 0.15 +2.3 3,416,389 - Pf6N2E2_2923 0.15 +0.9 3,416,389 - Pf6N2E2_2923 0.15 +1.6 3,416,389 - Pf6N2E2_2923 0.15 +1.6 3,416,389 - Pf6N2E2_2923 0.15 +2.0 3,416,389 - Pf6N2E2_2923 0.15 -0.0 3,416,389 - Pf6N2E2_2923 0.15 +1.9 3,416,389 - Pf6N2E2_2923 0.15 +2.2 3,416,389 - Pf6N2E2_2923 0.15 +1.9 3,416,389 - Pf6N2E2_2923 0.15 +1.6 3,416,389 - Pf6N2E2_2923 0.15 +1.2 3,416,389 - Pf6N2E2_2923 0.15 +0.7 3,416,403 + Pf6N2E2_2923 0.16 +1.7 3,416,404 - Pf6N2E2_2923 0.16 +2.6 3,416,404 - Pf6N2E2_2923 0.16 +1.6 3,416,404 - Pf6N2E2_2923 0.16 +1.7 3,416,404 - Pf6N2E2_2923 0.16 +2.3 3,416,404 - Pf6N2E2_2923 0.16 +0.3 3,416,508 + Pf6N2E2_2923 0.28 -1.2 3,416,712 + Pf6N2E2_2923 0.50 +1.4 3,416,712 + Pf6N2E2_2923 0.50 +2.9 3,416,712 + Pf6N2E2_2923 0.50 +2.5 3,416,928 + Pf6N2E2_2923 0.73 +1.5 3,416,929 - Pf6N2E2_2923 0.73 +0.7 3,417,084 + +1.7 3,417,084 + +1.6 3,417,084 + +1.0 3,417,085 - +2.1 3,417,085 - +1.9 3,417,172 + +0.6 3,417,172 + +1.2 3,417,172 + +2.1 3,417,173 - +2.8 3,417,173 - +2.0 3,417,173 - +2.5 3,417,178 + +1.7 3,417,179 - +1.4 3,417,179 - +1.9 3,417,179 - +2.0 3,417,179 - +0.8 3,417,179 - +1.1 3,417,247 + +1.7 3,417,248 - +1.3 3,417,248 - +1.4 3,417,265 + +1.2 3,417,539 + Pf6N2E2_2924 0.29 +0.2 3,417,539 + Pf6N2E2_2924 0.29 +1.7 3,417,539 + Pf6N2E2_2924 0.29 +1.0 3,417,539 + Pf6N2E2_2924 0.29 +1.8 3,417,539 + Pf6N2E2_2924 0.29 +1.5 3,417,539 + Pf6N2E2_2924 0.29 +0.8 3,417,540 - Pf6N2E2_2924 0.29 +2.2 3,417,540 - Pf6N2E2_2924 0.29 +2.6 3,417,540 - Pf6N2E2_2924 0.29 +2.4 3,417,574 - Pf6N2E2_2924 0.32 +2.5 3,417,614 + Pf6N2E2_2924 0.35 +1.3 3,417,614 + Pf6N2E2_2924 0.35 +0.4 3,417,614 + Pf6N2E2_2924 0.35 +1.5 3,417,630 - Pf6N2E2_2924 0.36 +1.6 3,417,630 - Pf6N2E2_2924 0.36 +0.9 3,417,635 + Pf6N2E2_2924 0.37 +1.7 3,417,635 + Pf6N2E2_2924 0.37 +1.7 3,417,635 + Pf6N2E2_2924 0.37 +1.7 3,417,636 - Pf6N2E2_2924 0.37 +2.9 3,417,636 - Pf6N2E2_2924 0.37 +1.2 3,417,636 - Pf6N2E2_2924 0.37 +1.4 3,417,636 - Pf6N2E2_2924 0.37 +0.9 3,417,653 + Pf6N2E2_2924 0.38 +1.9 3,417,653 + Pf6N2E2_2924 0.38 +2.7 3,417,654 - Pf6N2E2_2924 0.38 +0.7 3,417,654 - Pf6N2E2_2924 0.38 +2.1 3,417,756 - Pf6N2E2_2924 0.46 +2.2 3,417,756 - Pf6N2E2_2924 0.46 -0.1 3,417,768 + Pf6N2E2_2924 0.47 +1.5 3,417,768 + Pf6N2E2_2924 0.47 +1.8 3,417,890 - Pf6N2E2_2924 0.57 +2.0 3,417,924 - Pf6N2E2_2924 0.60 +2.1 3,417,924 - Pf6N2E2_2924 0.60 +1.1 3,417,938 + Pf6N2E2_2924 0.61 +1.5 3,417,938 + Pf6N2E2_2924 0.61 +2.3 3,417,938 + Pf6N2E2_2924 0.61 +1.3 3,417,938 + Pf6N2E2_2924 0.61 +2.0 3,417,938 + Pf6N2E2_2924 0.61 +1.8 3,417,938 + Pf6N2E2_2924 0.61 -1.1 3,417,939 - Pf6N2E2_2924 0.61 +1.1 3,417,939 - Pf6N2E2_2924 0.61 +1.8 3,417,939 - Pf6N2E2_2924 0.61 +1.7 3,417,969 - Pf6N2E2_2924 0.63 +1.9 3,417,969 - Pf6N2E2_2924 0.63 +1.2 3,417,969 - Pf6N2E2_2924 0.63 +2.0 3,418,397 - +1.3 3,418,588 + Pf6N2E2_2925 0.21 +1.8 3,418,588 + Pf6N2E2_2925 0.21 +0.5 3,418,588 + Pf6N2E2_2925 0.21 +2.0 3,418,588 + Pf6N2E2_2925 0.21 +1.7 3,418,641 - Pf6N2E2_2925 0.28 +1.7 3,418,673 + Pf6N2E2_2925 0.32 +1.3 3,418,822 + Pf6N2E2_2925 0.52 +1.6 3,419,191 + -1.7 3,419,191 + +1.7 3,419,191 + +1.6 3,419,191 + -0.7 3,419,192 - +1.4 3,419,225 - +1.1 3,419,371 + Pf6N2E2_2926 0.25 +1.6 3,419,371 + Pf6N2E2_2926 0.25 +1.2
Or see this region's nucleotide sequence