Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2658 and Pf6N2E2_2659 are separated by 252 nucleotides Pf6N2E2_2659 and Pf6N2E2_2660 are separated by 82 nucleotides Pf6N2E2_2660 and Pf6N2E2_2661 are separated by 103 nucleotides
Pf6N2E2_2658 - sodium-solute symporter, putative, at 3,130,966 to 3,132,354
_2658
Pf6N2E2_2659 - hypothetical protein, at 3,132,607 to 3,132,777
_2659
Pf6N2E2_2660 - Transcriptional regulators, LysR family, at 3,132,860 to 3,133,753
_2660
Pf6N2E2_2661 - Possible MFS Superfamily transporter precursor, at 3,133,857 to 3,135,029
_2661
Position (kb)
3132
3133
3134 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3132.112 kb on + strand, within Pf6N2E2_2658 at 3132.112 kb on + strand, within Pf6N2E2_2658 at 3132.112 kb on + strand, within Pf6N2E2_2658 at 3132.112 kb on + strand, within Pf6N2E2_2658 at 3132.112 kb on + strand, within Pf6N2E2_2658 at 3132.160 kb on + strand, within Pf6N2E2_2658 at 3132.160 kb on + strand, within Pf6N2E2_2658 at 3132.268 kb on + strand at 3132.268 kb on + strand at 3132.268 kb on + strand at 3132.269 kb on - strand at 3132.269 kb on - strand at 3132.271 kb on + strand at 3132.271 kb on + strand at 3132.272 kb on - strand at 3132.352 kb on + strand at 3132.353 kb on - strand at 3132.511 kb on + strand at 3132.512 kb on - strand at 3132.519 kb on - strand at 3132.589 kb on + strand at 3132.589 kb on + strand at 3132.590 kb on - strand at 3132.590 kb on - strand at 3132.590 kb on - strand at 3132.590 kb on - strand at 3132.590 kb on - strand at 3132.590 kb on - strand at 3132.756 kb on + strand, within Pf6N2E2_2659 at 3132.756 kb on + strand, within Pf6N2E2_2659 at 3132.778 kb on + strand at 3132.779 kb on - strand at 3132.782 kb on - strand at 3132.782 kb on - strand at 3132.782 kb on - strand at 3132.794 kb on + strand at 3133.101 kb on + strand, within Pf6N2E2_2660 at 3133.101 kb on + strand, within Pf6N2E2_2660 at 3133.102 kb on - strand, within Pf6N2E2_2660 at 3133.102 kb on - strand, within Pf6N2E2_2660 at 3133.262 kb on + strand, within Pf6N2E2_2660 at 3133.263 kb on - strand, within Pf6N2E2_2660 at 3133.422 kb on + strand, within Pf6N2E2_2660 at 3133.423 kb on - strand, within Pf6N2E2_2660 at 3133.423 kb on - strand, within Pf6N2E2_2660 at 3133.464 kb on + strand, within Pf6N2E2_2660 at 3133.464 kb on + strand, within Pf6N2E2_2660 at 3133.464 kb on + strand, within Pf6N2E2_2660 at 3133.464 kb on + strand, within Pf6N2E2_2660 at 3133.464 kb on + strand, within Pf6N2E2_2660 at 3133.464 kb on + strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.465 kb on - strand, within Pf6N2E2_2660 at 3133.555 kb on - strand, within Pf6N2E2_2660 at 3133.819 kb on - strand at 3133.867 kb on + strand at 3133.868 kb on - strand at 3133.868 kb on - strand at 3133.942 kb on + strand at 3133.942 kb on + strand at 3133.943 kb on - strand at 3133.943 kb on - strand at 3133.943 kb on - strand at 3133.943 kb on - strand at 3134.007 kb on + strand, within Pf6N2E2_2661 at 3134.007 kb on + strand, within Pf6N2E2_2661 at 3134.007 kb on + strand, within Pf6N2E2_2661 at 3134.007 kb on + strand, within Pf6N2E2_2661 at 3134.007 kb on + strand, within Pf6N2E2_2661 at 3134.007 kb on + strand, within Pf6N2E2_2661 at 3134.008 kb on - strand, within Pf6N2E2_2661 at 3134.008 kb on - strand, within Pf6N2E2_2661 at 3134.008 kb on - strand, within Pf6N2E2_2661 at 3134.016 kb on + strand, within Pf6N2E2_2661 at 3134.016 kb on + strand, within Pf6N2E2_2661 at 3134.016 kb on + strand, within Pf6N2E2_2661 at 3134.017 kb on - strand, within Pf6N2E2_2661 at 3134.017 kb on - strand, within Pf6N2E2_2661 at 3134.356 kb on - strand, within Pf6N2E2_2661 at 3134.356 kb on - strand, within Pf6N2E2_2661 at 3134.484 kb on + strand, within Pf6N2E2_2661 at 3134.484 kb on + strand, within Pf6N2E2_2661 at 3134.484 kb on + strand, within Pf6N2E2_2661 at 3134.485 kb on - strand, within Pf6N2E2_2661 at 3134.485 kb on - strand, within Pf6N2E2_2661 at 3134.494 kb on - strand, within Pf6N2E2_2661 at 3134.505 kb on + strand, within Pf6N2E2_2661 at 3134.505 kb on + strand, within Pf6N2E2_2661 at 3134.506 kb on - strand, within Pf6N2E2_2661 at 3134.506 kb on - strand, within Pf6N2E2_2661 at 3134.699 kb on - strand, within Pf6N2E2_2661
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,132,112 + Pf6N2E2_2658 0.83 +0.8 3,132,112 + Pf6N2E2_2658 0.83 +1.5 3,132,112 + Pf6N2E2_2658 0.83 -2.4 3,132,112 + Pf6N2E2_2658 0.83 +0.2 3,132,112 + Pf6N2E2_2658 0.83 -0.4 3,132,160 + Pf6N2E2_2658 0.86 +2.2 3,132,160 + Pf6N2E2_2658 0.86 -0.2 3,132,268 + -0.6 3,132,268 + +0.1 3,132,268 + -1.1 3,132,269 - +0.2 3,132,269 - +0.1 3,132,271 + +0.9 3,132,271 + +0.9 3,132,272 - -0.3 3,132,352 + +0.6 3,132,353 - +1.3 3,132,511 + -0.8 3,132,512 - +1.3 3,132,519 - +2.3 3,132,589 + +0.3 3,132,589 + -0.6 3,132,590 - -0.4 3,132,590 - -0.1 3,132,590 - +0.3 3,132,590 - +0.5 3,132,590 - -0.2 3,132,590 - +0.4 3,132,756 + Pf6N2E2_2659 0.87 +0.6 3,132,756 + Pf6N2E2_2659 0.87 -0.3 3,132,778 + +1.0 3,132,779 - -0.2 3,132,782 - +0.1 3,132,782 - -1.1 3,132,782 - -0.6 3,132,794 + +2.4 3,133,101 + Pf6N2E2_2660 0.27 -0.5 3,133,101 + Pf6N2E2_2660 0.27 -2.6 3,133,102 - Pf6N2E2_2660 0.27 -2.9 3,133,102 - Pf6N2E2_2660 0.27 +0.3 3,133,262 + Pf6N2E2_2660 0.45 +2.2 3,133,263 - Pf6N2E2_2660 0.45 -0.2 3,133,422 + Pf6N2E2_2660 0.63 -0.3 3,133,423 - Pf6N2E2_2660 0.63 -1.2 3,133,423 - Pf6N2E2_2660 0.63 +0.1 3,133,464 + Pf6N2E2_2660 0.68 -0.7 3,133,464 + Pf6N2E2_2660 0.68 +1.4 3,133,464 + Pf6N2E2_2660 0.68 +1.2 3,133,464 + Pf6N2E2_2660 0.68 +0.4 3,133,464 + Pf6N2E2_2660 0.68 -0.5 3,133,464 + Pf6N2E2_2660 0.68 +1.0 3,133,465 - Pf6N2E2_2660 0.68 -2.5 3,133,465 - Pf6N2E2_2660 0.68 +0.5 3,133,465 - Pf6N2E2_2660 0.68 -1.1 3,133,465 - Pf6N2E2_2660 0.68 -0.8 3,133,465 - Pf6N2E2_2660 0.68 +0.2 3,133,465 - Pf6N2E2_2660 0.68 -1.1 3,133,465 - Pf6N2E2_2660 0.68 -1.9 3,133,465 - Pf6N2E2_2660 0.68 +0.1 3,133,555 - Pf6N2E2_2660 0.78 +1.1 3,133,819 - +0.1 3,133,867 + +0.5 3,133,868 - -1.5 3,133,868 - -0.2 3,133,942 + -3.1 3,133,942 + +0.4 3,133,943 - -0.3 3,133,943 - +0.4 3,133,943 - -1.2 3,133,943 - -0.1 3,134,007 + Pf6N2E2_2661 0.13 +0.9 3,134,007 + Pf6N2E2_2661 0.13 +0.1 3,134,007 + Pf6N2E2_2661 0.13 -0.8 3,134,007 + Pf6N2E2_2661 0.13 -0.8 3,134,007 + Pf6N2E2_2661 0.13 +0.1 3,134,007 + Pf6N2E2_2661 0.13 -0.8 3,134,008 - Pf6N2E2_2661 0.13 +0.6 3,134,008 - Pf6N2E2_2661 0.13 -1.4 3,134,008 - Pf6N2E2_2661 0.13 -0.2 3,134,016 + Pf6N2E2_2661 0.14 -0.1 3,134,016 + Pf6N2E2_2661 0.14 -0.4 3,134,016 + Pf6N2E2_2661 0.14 +0.7 3,134,017 - Pf6N2E2_2661 0.14 -0.3 3,134,017 - Pf6N2E2_2661 0.14 +0.4 3,134,356 - Pf6N2E2_2661 0.43 +0.6 3,134,356 - Pf6N2E2_2661 0.43 +0.1 3,134,484 + Pf6N2E2_2661 0.53 +1.8 3,134,484 + Pf6N2E2_2661 0.53 +0.7 3,134,484 + Pf6N2E2_2661 0.53 +0.1 3,134,485 - Pf6N2E2_2661 0.54 -0.5 3,134,485 - Pf6N2E2_2661 0.54 -0.6 3,134,494 - Pf6N2E2_2661 0.54 -0.3 3,134,505 + Pf6N2E2_2661 0.55 +1.2 3,134,505 + Pf6N2E2_2661 0.55 +0.2 3,134,506 - Pf6N2E2_2661 0.55 -0.1 3,134,506 - Pf6N2E2_2661 0.55 +0.5 3,134,699 - Pf6N2E2_2661 0.72 -2.9
Or see this region's nucleotide sequence