Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_257 and Pf6N2E2_258 are separated by 145 nucleotides Pf6N2E2_258 and Pf6N2E2_259 are separated by 238 nucleotides
Pf6N2E2_257 - Exoenzymes regulatory protein AepA precursor, at 320,354 to 322,192
_257
Pf6N2E2_258 - Nicotinamidase family protein YcaC, at 322,338 to 322,967
_258
Pf6N2E2_259 - Transcriptional regulator, LysR family, at 323,206 to 324,084
_259
Position (kb) 322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 321.383 kb on + strand, within Pf6N2E2_257 at 321.383 kb on + strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.579 kb on - strand, within Pf6N2E2_257 at 321.579 kb on - strand, within Pf6N2E2_257 at 321.677 kb on + strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.699 kb on - strand, within Pf6N2E2_257 at 321.947 kb on + strand, within Pf6N2E2_257 at 321.948 kb on - strand, within Pf6N2E2_257 at 322.311 kb on + strand at 322.312 kb on - strand at 322.312 kb on - strand at 322.356 kb on + strand at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.767 kb on - strand, within Pf6N2E2_258 at 322.944 kb on + strand at 322.944 kb on + strand at 322.945 kb on - strand at 322.945 kb on - strand at 322.965 kb on + strand at 322.966 kb on - strand at 322.966 kb on - strand at 322.966 kb on - strand at 322.966 kb on - strand at 323.026 kb on + strand at 323.027 kb on - strand at 323.027 kb on - strand at 323.047 kb on + strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.620 kb on + strand, within Pf6N2E2_259 at 323.620 kb on + strand, within Pf6N2E2_259 at 323.620 kb on + strand, within Pf6N2E2_259 at 323.621 kb on - strand, within Pf6N2E2_259 at 323.698 kb on + strand, within Pf6N2E2_259 at 323.698 kb on + strand, within Pf6N2E2_259 at 323.699 kb on - strand, within Pf6N2E2_259 at 323.716 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 321,383 + Pf6N2E2_257 0.56 -0.2 321,383 + Pf6N2E2_257 0.56 +0.3 321,384 - Pf6N2E2_257 0.56 +0.9 321,384 - Pf6N2E2_257 0.56 +0.7 321,384 - Pf6N2E2_257 0.56 -1.0 321,384 - Pf6N2E2_257 0.56 +0.6 321,384 - Pf6N2E2_257 0.56 -0.4 321,384 - Pf6N2E2_257 0.56 +0.5 321,579 - Pf6N2E2_257 0.67 -0.5 321,579 - Pf6N2E2_257 0.67 +0.8 321,677 + Pf6N2E2_257 0.72 +1.1 321,678 - Pf6N2E2_257 0.72 -0.6 321,678 - Pf6N2E2_257 0.72 +1.0 321,678 - Pf6N2E2_257 0.72 +1.5 321,699 - Pf6N2E2_257 0.73 -0.9 321,947 + Pf6N2E2_257 0.87 +1.7 321,948 - Pf6N2E2_257 0.87 +0.7 322,311 + -1.3 322,312 - +0.8 322,312 - +0.5 322,356 + +1.0 322,584 + Pf6N2E2_258 0.39 +0.1 322,584 + Pf6N2E2_258 0.39 +0.2 322,584 + Pf6N2E2_258 0.39 -1.0 322,584 + Pf6N2E2_258 0.39 -0.2 322,584 + Pf6N2E2_258 0.39 +0.8 322,584 + Pf6N2E2_258 0.39 +1.0 322,767 - Pf6N2E2_258 0.68 +1.5 322,944 + +0.1 322,944 + -1.1 322,945 - +0.8 322,945 - +0.5 322,965 + +2.0 322,966 - -1.5 322,966 - +0.0 322,966 - +0.8 322,966 - -0.3 323,026 + +0.1 323,027 - +0.9 323,027 - -1.8 323,047 + -0.8 323,194 - -0.6 323,194 - -0.6 323,620 + Pf6N2E2_259 0.47 -0.1 323,620 + Pf6N2E2_259 0.47 +0.3 323,620 + Pf6N2E2_259 0.47 +0.8 323,621 - Pf6N2E2_259 0.47 -0.7 323,698 + Pf6N2E2_259 0.56 +0.2 323,698 + Pf6N2E2_259 0.56 -0.5 323,699 - Pf6N2E2_259 0.56 +0.6 323,716 + Pf6N2E2_259 0.58 -0.4 323,914 + Pf6N2E2_259 0.81 +1.6 323,914 + Pf6N2E2_259 0.81 -1.6 323,914 + Pf6N2E2_259 0.81 +0.6 323,915 - Pf6N2E2_259 0.81 +1.0 323,915 - Pf6N2E2_259 0.81 -0.6 323,915 - Pf6N2E2_259 0.81 -0.0 323,915 - Pf6N2E2_259 0.81 -1.2 323,915 - Pf6N2E2_259 0.81 -0.3
Or see this region's nucleotide sequence