Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2536 and Pf6N2E2_2537 are separated by 133 nucleotides Pf6N2E2_2537 and Pf6N2E2_2538 are separated by 172 nucleotides Pf6N2E2_2538 and Pf6N2E2_2539 are separated by 151 nucleotides
Pf6N2E2_2536 - Fumarate hydratase class II (EC 4.2.1.2), at 2,994,319 to 2,995,713
_2536
Pf6N2E2_2537 - membrane protein, putative, at 2,995,847 to 2,996,827
_2537
Pf6N2E2_2538 - hypothetical protein, at 2,997,000 to 2,997,197
_2538
Pf6N2E2_2539 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 2,997,349 to 2,998,533
_2539
Position (kb)
2995
2996
2997 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2994.871 kb on - strand, within Pf6N2E2_2536 at 2994.941 kb on - strand, within Pf6N2E2_2536 at 2995.597 kb on + strand at 2995.643 kb on - strand at 2995.686 kb on + strand at 2995.686 kb on + strand at 2995.687 kb on - strand at 2995.687 kb on - strand at 2995.687 kb on - strand at 2995.780 kb on + strand at 2995.849 kb on - strand at 2995.986 kb on + strand, within Pf6N2E2_2537 at 2995.986 kb on + strand, within Pf6N2E2_2537 at 2995.987 kb on - strand, within Pf6N2E2_2537 at 2995.987 kb on - strand, within Pf6N2E2_2537 at 2995.987 kb on - strand, within Pf6N2E2_2537 at 2995.987 kb on - strand, within Pf6N2E2_2537 at 2995.987 kb on - strand, within Pf6N2E2_2537 at 2995.987 kb on - strand, within Pf6N2E2_2537 at 2996.221 kb on - strand, within Pf6N2E2_2537 at 2996.221 kb on - strand, within Pf6N2E2_2537 at 2996.221 kb on - strand, within Pf6N2E2_2537 at 2996.221 kb on - strand, within Pf6N2E2_2537 at 2996.283 kb on + strand, within Pf6N2E2_2537 at 2996.283 kb on + strand, within Pf6N2E2_2537 at 2996.283 kb on + strand, within Pf6N2E2_2537 at 2996.284 kb on - strand, within Pf6N2E2_2537 at 2996.284 kb on - strand, within Pf6N2E2_2537 at 2996.284 kb on - strand, within Pf6N2E2_2537 at 2996.284 kb on - strand, within Pf6N2E2_2537 at 2996.284 kb on - strand, within Pf6N2E2_2537 at 2996.334 kb on + strand, within Pf6N2E2_2537 at 2996.334 kb on + strand, within Pf6N2E2_2537 at 2996.335 kb on - strand, within Pf6N2E2_2537 at 2996.387 kb on - strand, within Pf6N2E2_2537 at 2996.538 kb on + strand, within Pf6N2E2_2537 at 2996.539 kb on - strand, within Pf6N2E2_2537 at 2996.547 kb on + strand, within Pf6N2E2_2537 at 2996.547 kb on + strand, within Pf6N2E2_2537 at 2996.547 kb on + strand, within Pf6N2E2_2537 at 2996.547 kb on + strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.548 kb on - strand, within Pf6N2E2_2537 at 2996.643 kb on + strand, within Pf6N2E2_2537 at 2996.643 kb on + strand, within Pf6N2E2_2537 at 2996.643 kb on + strand, within Pf6N2E2_2537 at 2996.644 kb on - strand, within Pf6N2E2_2537 at 2996.801 kb on + strand at 2996.801 kb on + strand at 2996.801 kb on + strand at 2996.801 kb on + strand at 2996.801 kb on + strand at 2996.801 kb on + strand at 2996.801 kb on + strand at 2996.846 kb on + strand at 2996.846 kb on + strand at 2996.847 kb on - strand at 2996.847 kb on - strand at 2996.854 kb on + strand at 2996.854 kb on + strand at 2996.854 kb on + strand at 2996.855 kb on - strand at 2996.855 kb on - strand at 2996.904 kb on + strand at 2996.904 kb on + strand at 2996.904 kb on + strand at 2996.904 kb on + strand at 2996.904 kb on + strand at 2996.905 kb on - strand at 2996.905 kb on - strand at 2996.905 kb on - strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.956 kb on + strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2996.957 kb on - strand at 2997.038 kb on + strand, within Pf6N2E2_2538 at 2997.148 kb on + strand, within Pf6N2E2_2538 at 2997.148 kb on + strand, within Pf6N2E2_2538 at 2997.149 kb on - strand, within Pf6N2E2_2538 at 2997.149 kb on - strand, within Pf6N2E2_2538 at 2997.149 kb on - strand, within Pf6N2E2_2538 at 2997.149 kb on - strand, within Pf6N2E2_2538 at 2997.156 kb on + strand, within Pf6N2E2_2538 at 2997.156 kb on + strand, within Pf6N2E2_2538 at 2997.156 kb on + strand, within Pf6N2E2_2538 at 2997.156 kb on + strand, within Pf6N2E2_2538 at 2997.156 kb on + strand, within Pf6N2E2_2538 at 2997.157 kb on - strand, within Pf6N2E2_2538 at 2997.157 kb on - strand at 2997.157 kb on - strand, within Pf6N2E2_2538 at 2997.157 kb on - strand, within Pf6N2E2_2538 at 2997.172 kb on + strand, within Pf6N2E2_2538 at 2997.172 kb on + strand, within Pf6N2E2_2538 at 2997.196 kb on - strand at 2997.264 kb on - strand at 2997.264 kb on - strand at 2997.264 kb on - strand at 2997.269 kb on + strand at 2997.406 kb on + strand at 2997.521 kb on + strand, within Pf6N2E2_2539 at 2997.521 kb on + strand, within Pf6N2E2_2539 at 2997.522 kb on - strand, within Pf6N2E2_2539 at 2997.522 kb on - strand, within Pf6N2E2_2539 at 2997.522 kb on - strand, within Pf6N2E2_2539 at 2997.522 kb on - strand, within Pf6N2E2_2539 at 2997.522 kb on - strand, within Pf6N2E2_2539 at 2997.558 kb on - strand, within Pf6N2E2_2539 at 2997.558 kb on - strand, within Pf6N2E2_2539 at 2997.753 kb on + strand, within Pf6N2E2_2539
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,994,871 - Pf6N2E2_2536 0.40 +0.5 2,994,941 - Pf6N2E2_2536 0.45 -1.3 2,995,597 + -0.3 2,995,643 - +0.7 2,995,686 + +0.2 2,995,686 + -0.4 2,995,687 - -0.2 2,995,687 - -0.4 2,995,687 - -2.4 2,995,780 + -0.5 2,995,849 - -0.0 2,995,986 + Pf6N2E2_2537 0.14 +1.2 2,995,986 + Pf6N2E2_2537 0.14 +1.5 2,995,987 - Pf6N2E2_2537 0.14 +0.1 2,995,987 - Pf6N2E2_2537 0.14 -0.2 2,995,987 - Pf6N2E2_2537 0.14 -2.9 2,995,987 - Pf6N2E2_2537 0.14 -0.9 2,995,987 - Pf6N2E2_2537 0.14 -0.5 2,995,987 - Pf6N2E2_2537 0.14 -0.7 2,996,221 - Pf6N2E2_2537 0.38 -0.1 2,996,221 - Pf6N2E2_2537 0.38 -0.2 2,996,221 - Pf6N2E2_2537 0.38 +1.5 2,996,221 - Pf6N2E2_2537 0.38 -0.8 2,996,283 + Pf6N2E2_2537 0.44 -0.6 2,996,283 + Pf6N2E2_2537 0.44 +0.2 2,996,283 + Pf6N2E2_2537 0.44 +0.4 2,996,284 - Pf6N2E2_2537 0.45 -0.4 2,996,284 - Pf6N2E2_2537 0.45 +1.8 2,996,284 - Pf6N2E2_2537 0.45 -1.2 2,996,284 - Pf6N2E2_2537 0.45 +0.1 2,996,284 - Pf6N2E2_2537 0.45 -0.7 2,996,334 + Pf6N2E2_2537 0.50 +0.5 2,996,334 + Pf6N2E2_2537 0.50 -0.2 2,996,335 - Pf6N2E2_2537 0.50 -0.2 2,996,387 - Pf6N2E2_2537 0.55 -0.7 2,996,538 + Pf6N2E2_2537 0.70 +1.3 2,996,539 - Pf6N2E2_2537 0.71 +0.3 2,996,547 + Pf6N2E2_2537 0.71 -0.9 2,996,547 + Pf6N2E2_2537 0.71 +0.8 2,996,547 + Pf6N2E2_2537 0.71 -0.7 2,996,547 + Pf6N2E2_2537 0.71 -0.3 2,996,548 - Pf6N2E2_2537 0.71 +1.2 2,996,548 - Pf6N2E2_2537 0.71 -1.2 2,996,548 - Pf6N2E2_2537 0.71 -2.6 2,996,548 - Pf6N2E2_2537 0.71 +0.3 2,996,548 - Pf6N2E2_2537 0.71 -2.5 2,996,548 - Pf6N2E2_2537 0.71 -0.3 2,996,548 - Pf6N2E2_2537 0.71 +1.2 2,996,548 - Pf6N2E2_2537 0.71 -1.0 2,996,548 - Pf6N2E2_2537 0.71 +0.4 2,996,548 - Pf6N2E2_2537 0.71 -0.6 2,996,548 - Pf6N2E2_2537 0.71 -0.6 2,996,643 + Pf6N2E2_2537 0.81 -0.1 2,996,643 + Pf6N2E2_2537 0.81 -1.2 2,996,643 + Pf6N2E2_2537 0.81 -0.5 2,996,644 - Pf6N2E2_2537 0.81 +0.7 2,996,801 + -0.0 2,996,801 + +1.1 2,996,801 + +0.6 2,996,801 + -0.2 2,996,801 + +0.6 2,996,801 + +0.5 2,996,801 + -0.3 2,996,846 + +0.0 2,996,846 + -1.3 2,996,847 - -0.2 2,996,847 - +0.6 2,996,854 + +0.3 2,996,854 + +1.3 2,996,854 + -0.7 2,996,855 - +1.3 2,996,855 - -0.2 2,996,904 + +1.2 2,996,904 + +0.4 2,996,904 + +0.7 2,996,904 + -0.2 2,996,904 + +0.3 2,996,905 - +0.2 2,996,905 - +1.8 2,996,905 - +1.3 2,996,956 + -0.5 2,996,956 + +0.4 2,996,956 + +1.4 2,996,956 + +0.5 2,996,956 + -0.9 2,996,956 + -0.0 2,996,956 + -0.3 2,996,956 + -1.2 2,996,956 + +1.8 2,996,956 + -0.7 2,996,956 + +0.4 2,996,956 + +1.0 2,996,956 + +0.9 2,996,956 + +0.6 2,996,956 + -0.8 2,996,956 + -0.4 2,996,956 + +0.4 2,996,956 + -0.8 2,996,956 + -2.0 2,996,957 - -0.4 2,996,957 - -0.2 2,996,957 - +0.1 2,996,957 - +0.1 2,996,957 - -0.1 2,996,957 - +1.4 2,996,957 - +0.1 2,996,957 - -0.0 2,996,957 - -3.7 2,996,957 - +0.6 2,996,957 - +0.0 2,996,957 - +1.1 2,996,957 - +0.0 2,996,957 - -0.4 2,996,957 - -0.2 2,996,957 - -0.0 2,996,957 - -1.1 2,996,957 - +1.2 2,996,957 - -1.0 2,996,957 - -0.0 2,996,957 - -0.5 2,997,038 + Pf6N2E2_2538 0.19 +0.6 2,997,148 + Pf6N2E2_2538 0.75 +0.1 2,997,148 + Pf6N2E2_2538 0.75 -1.2 2,997,149 - Pf6N2E2_2538 0.75 +0.2 2,997,149 - Pf6N2E2_2538 0.75 +0.6 2,997,149 - Pf6N2E2_2538 0.75 +0.1 2,997,149 - Pf6N2E2_2538 0.75 -1.2 2,997,156 + Pf6N2E2_2538 0.79 -3.7 2,997,156 + Pf6N2E2_2538 0.79 -0.5 2,997,156 + Pf6N2E2_2538 0.79 +0.4 2,997,156 + Pf6N2E2_2538 0.79 -0.6 2,997,156 + Pf6N2E2_2538 0.79 +0.3 2,997,157 - Pf6N2E2_2538 0.79 +1.6 2,997,157 - -1.2 2,997,157 - Pf6N2E2_2538 0.79 -3.0 2,997,157 - Pf6N2E2_2538 0.79 -1.8 2,997,172 + Pf6N2E2_2538 0.87 +0.0 2,997,172 + Pf6N2E2_2538 0.87 +0.7 2,997,196 - -0.4 2,997,264 - -0.1 2,997,264 - +0.2 2,997,264 - -0.3 2,997,269 + +0.6 2,997,406 + -0.4 2,997,521 + Pf6N2E2_2539 0.15 +0.3 2,997,521 + Pf6N2E2_2539 0.15 +1.0 2,997,522 - Pf6N2E2_2539 0.15 -0.5 2,997,522 - Pf6N2E2_2539 0.15 +0.3 2,997,522 - Pf6N2E2_2539 0.15 +1.0 2,997,522 - Pf6N2E2_2539 0.15 -0.4 2,997,522 - Pf6N2E2_2539 0.15 +0.3 2,997,558 - Pf6N2E2_2539 0.18 +1.7 2,997,558 - Pf6N2E2_2539 0.18 -1.1 2,997,753 + Pf6N2E2_2539 0.34 -0.4
Or see this region's nucleotide sequence