Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6109 and Pf6N2E2_6110 are separated by 61 nucleotides Pf6N2E2_6110 and Pf6N2E2_6111 are separated by 44 nucleotides Pf6N2E2_6111 and Pf6N2E2_2221 are separated by 281 nucleotides Pf6N2E2_2221 and Pf6N2E2_2222 are separated by 275 nucleotides
Pf6N2E2_6109 - tRNA-Leu-TAG, at 2,635,014 to 2,635,095
_6109
Pf6N2E2_6110 - tRNA-His-GTG, at 2,635,157 to 2,635,229
_6110
Pf6N2E2_6111 - tRNA-Arg-TCT, at 2,635,274 to 2,635,347
_6111
Pf6N2E2_2221 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 2,635,629 to 2,636,483
_2221
Pf6N2E2_2222 - Murein-DD-endopeptidase (EC 3.4.99.-), at 2,636,759 to 2,637,736
_2222
Position (kb)
2635
2636
2637 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2634.653 kb on + strand at 2634.653 kb on + strand at 2634.653 kb on + strand at 2634.654 kb on - strand at 2634.654 kb on - strand at 2634.654 kb on - strand at 2634.656 kb on + strand at 2634.713 kb on + strand at 2634.753 kb on + strand at 2634.754 kb on - strand at 2634.754 kb on - strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.791 kb on + strand at 2634.792 kb on - strand at 2634.792 kb on - strand at 2634.792 kb on - strand at 2634.792 kb on - strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.793 kb on + strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.794 kb on - strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.795 kb on + strand at 2634.796 kb on - strand at 2634.796 kb on - strand at 2634.796 kb on - strand at 2634.796 kb on - strand at 2634.796 kb on - strand at 2634.796 kb on - strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.797 kb on + strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.798 kb on - strand at 2634.896 kb on + strand at 2634.992 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2634.996 kb on - strand at 2635.010 kb on - strand at 2635.010 kb on - strand at 2635.010 kb on - strand at 2635.131 kb on - strand at 2635.131 kb on - strand at 2635.148 kb on - strand at 2635.148 kb on - strand at 2635.148 kb on - strand at 2636.627 kb on - strand at 2636.678 kb on + strand at 2636.679 kb on - strand at 2636.679 kb on - strand at 2636.679 kb on - strand at 2636.684 kb on + strand at 2636.684 kb on + strand at 2636.684 kb on + strand at 2636.705 kb on + strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.707 kb on + strand at 2636.707 kb on + strand at 2636.707 kb on + strand at 2636.707 kb on + strand at 2636.708 kb on - strand at 2636.708 kb on - strand at 2636.725 kb on + strand at 2636.726 kb on - strand at 2636.791 kb on + strand at 2637.062 kb on + strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.285 kb on - strand, within Pf6N2E2_2222 at 2637.285 kb on - strand, within Pf6N2E2_2222 at 2637.366 kb on - strand, within Pf6N2E2_2222 at 2637.410 kb on - strand, within Pf6N2E2_2222 at 2637.428 kb on + strand, within Pf6N2E2_2222 at 2637.429 kb on - strand, within Pf6N2E2_2222
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,634,653 + +0.4 2,634,653 + +1.3 2,634,653 + +1.3 2,634,654 - +0.9 2,634,654 - +0.7 2,634,654 - -0.9 2,634,656 + -0.6 2,634,713 + +0.6 2,634,753 + +1.1 2,634,754 - -1.4 2,634,754 - -0.9 2,634,791 + +0.5 2,634,791 + +1.3 2,634,791 + +1.0 2,634,791 + +1.1 2,634,791 + +1.8 2,634,791 + +0.1 2,634,791 + -0.7 2,634,791 + +0.1 2,634,791 + -1.4 2,634,791 + -1.3 2,634,791 + -0.6 2,634,791 + -1.6 2,634,791 + +1.3 2,634,791 + +0.0 2,634,792 - +0.4 2,634,792 - +1.2 2,634,792 - +1.0 2,634,792 - +1.0 2,634,793 + -0.4 2,634,793 + -0.8 2,634,793 + +0.0 2,634,793 + +1.0 2,634,793 + +0.4 2,634,793 + -0.3 2,634,793 + -0.4 2,634,793 + +1.0 2,634,793 + +0.2 2,634,794 - +0.6 2,634,794 - -0.4 2,634,794 - +2.1 2,634,794 - -0.6 2,634,794 - -1.1 2,634,794 - +0.8 2,634,794 - +0.3 2,634,794 - -0.5 2,634,794 - -0.3 2,634,794 - +0.7 2,634,795 + -0.9 2,634,795 + -3.0 2,634,795 + -1.4 2,634,795 + +1.3 2,634,795 + +0.5 2,634,795 + +1.7 2,634,795 + +0.8 2,634,795 + -0.2 2,634,795 + -0.9 2,634,795 + +0.1 2,634,796 - +1.6 2,634,796 - -0.0 2,634,796 - +1.6 2,634,796 - -0.8 2,634,796 - -2.6 2,634,796 - +0.6 2,634,797 + +1.4 2,634,797 + -0.9 2,634,797 + +0.3 2,634,797 + +0.1 2,634,797 + +0.9 2,634,797 + -0.9 2,634,797 + +0.1 2,634,797 + -0.9 2,634,797 + -0.1 2,634,798 - -1.6 2,634,798 - +0.1 2,634,798 - +0.9 2,634,798 - +1.7 2,634,798 - -0.6 2,634,798 - +0.3 2,634,798 - +0.7 2,634,798 - +2.7 2,634,896 + +0.2 2,634,992 - +1.2 2,634,996 - -0.5 2,634,996 - -0.6 2,634,996 - -0.8 2,634,996 - -0.7 2,634,996 - +0.2 2,634,996 - +0.1 2,634,996 - -0.1 2,634,996 - -0.2 2,635,010 - -0.0 2,635,010 - -1.0 2,635,010 - +0.5 2,635,131 - -0.7 2,635,131 - -1.5 2,635,148 - -0.6 2,635,148 - +0.7 2,635,148 - +0.7 2,636,627 - +0.3 2,636,678 + +1.7 2,636,679 - +0.1 2,636,679 - -0.4 2,636,679 - +0.8 2,636,684 + -0.9 2,636,684 + -2.1 2,636,684 + -0.5 2,636,705 + +1.6 2,636,706 - -0.3 2,636,706 - -0.0 2,636,706 - -0.0 2,636,706 - +0.8 2,636,706 - -1.1 2,636,706 - -0.0 2,636,707 + +0.9 2,636,707 + +2.3 2,636,707 + -1.2 2,636,707 + +0.7 2,636,708 - -0.0 2,636,708 - -0.8 2,636,725 + -0.1 2,636,726 - +1.1 2,636,791 + +0.1 2,637,062 + Pf6N2E2_2222 0.31 +1.3 2,637,063 - Pf6N2E2_2222 0.31 +0.6 2,637,063 - Pf6N2E2_2222 0.31 +0.0 2,637,063 - Pf6N2E2_2222 0.31 +1.7 2,637,063 - Pf6N2E2_2222 0.31 +0.4 2,637,284 + Pf6N2E2_2222 0.54 +1.0 2,637,284 + Pf6N2E2_2222 0.54 -1.3 2,637,284 + Pf6N2E2_2222 0.54 +0.1 2,637,284 + Pf6N2E2_2222 0.54 -0.4 2,637,284 + Pf6N2E2_2222 0.54 -2.1 2,637,284 + Pf6N2E2_2222 0.54 -0.4 2,637,284 + Pf6N2E2_2222 0.54 -0.3 2,637,285 - Pf6N2E2_2222 0.54 +0.7 2,637,285 - Pf6N2E2_2222 0.54 -2.1 2,637,366 - Pf6N2E2_2222 0.62 +0.7 2,637,410 - Pf6N2E2_2222 0.67 -1.1 2,637,428 + Pf6N2E2_2222 0.68 -0.3 2,637,429 - Pf6N2E2_2222 0.69 -1.2
Or see this region's nucleotide sequence