Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1854 and Pf6N2E2_1855 are separated by 140 nucleotides Pf6N2E2_1855 and Pf6N2E2_1856 are separated by 26 nucleotides
Pf6N2E2_1854 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,198,114 to 2,199,562
_1854
Pf6N2E2_1855 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 2,199,703 to 2,200,854
_1855
Pf6N2E2_1856 - phytoene dehydrogenase family protein, at 2,200,881 to 2,202,287
_1856
Position (kb)
2199
2200
2201 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2198.757 kb on + strand, within Pf6N2E2_1854 at 2198.757 kb on + strand, within Pf6N2E2_1854 at 2198.757 kb on + strand, within Pf6N2E2_1854 at 2198.758 kb on - strand, within Pf6N2E2_1854 at 2198.758 kb on - strand, within Pf6N2E2_1854 at 2198.758 kb on - strand, within Pf6N2E2_1854 at 2199.288 kb on + strand, within Pf6N2E2_1854 at 2199.579 kb on - strand at 2199.702 kb on + strand at 2199.702 kb on + strand at 2199.703 kb on - strand at 2199.703 kb on - strand at 2199.961 kb on - strand, within Pf6N2E2_1855 at 2200.006 kb on + strand, within Pf6N2E2_1855 at 2200.007 kb on - strand, within Pf6N2E2_1855 at 2200.159 kb on + strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.418 kb on - strand, within Pf6N2E2_1855 at 2200.511 kb on - strand, within Pf6N2E2_1855 at 2200.511 kb on - strand, within Pf6N2E2_1855 at 2200.553 kb on + strand, within Pf6N2E2_1855 at 2200.553 kb on + strand, within Pf6N2E2_1855 at 2200.554 kb on - strand, within Pf6N2E2_1855 at 2200.637 kb on - strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.641 kb on - strand, within Pf6N2E2_1855 at 2200.641 kb on - strand, within Pf6N2E2_1855 at 2200.641 kb on - strand, within Pf6N2E2_1855 at 2200.747 kb on + strand at 2200.748 kb on - strand at 2200.748 kb on - strand at 2200.749 kb on + strand at 2200.749 kb on + strand at 2200.749 kb on + strand at 2200.750 kb on - strand at 2200.750 kb on - strand at 2200.750 kb on - strand at 2200.777 kb on + strand at 2200.777 kb on + strand at 2200.778 kb on - strand at 2200.804 kb on + strand at 2200.805 kb on - strand at 2200.895 kb on - strand at 2200.910 kb on - strand at 2201.071 kb on + strand, within Pf6N2E2_1856 at 2201.072 kb on - strand, within Pf6N2E2_1856 at 2201.072 kb on - strand, within Pf6N2E2_1856 at 2201.076 kb on - strand, within Pf6N2E2_1856 at 2201.144 kb on - strand, within Pf6N2E2_1856 at 2201.168 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.213 kb on + strand, within Pf6N2E2_1856 at 2201.213 kb on + strand, within Pf6N2E2_1856 at 2201.216 kb on - strand, within Pf6N2E2_1856 at 2201.222 kb on - strand, within Pf6N2E2_1856 at 2201.327 kb on - strand, within Pf6N2E2_1856 at 2201.398 kb on - strand, within Pf6N2E2_1856 at 2201.624 kb on - strand, within Pf6N2E2_1856 at 2201.676 kb on + strand, within Pf6N2E2_1856 at 2201.677 kb on - strand, within Pf6N2E2_1856 at 2201.800 kb on - strand, within Pf6N2E2_1856
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,198,757 + Pf6N2E2_1854 0.44 -1.0 2,198,757 + Pf6N2E2_1854 0.44 +0.9 2,198,757 + Pf6N2E2_1854 0.44 +0.5 2,198,758 - Pf6N2E2_1854 0.44 +0.5 2,198,758 - Pf6N2E2_1854 0.44 -0.4 2,198,758 - Pf6N2E2_1854 0.44 +0.3 2,199,288 + Pf6N2E2_1854 0.81 -0.2 2,199,579 - +0.4 2,199,702 + -0.2 2,199,702 + +0.5 2,199,703 - +0.7 2,199,703 - -0.3 2,199,961 - Pf6N2E2_1855 0.22 +0.5 2,200,006 + Pf6N2E2_1855 0.26 +1.1 2,200,007 - Pf6N2E2_1855 0.26 -0.5 2,200,159 + Pf6N2E2_1855 0.40 +1.9 2,200,160 - Pf6N2E2_1855 0.40 +0.4 2,200,160 - Pf6N2E2_1855 0.40 +1.0 2,200,160 - Pf6N2E2_1855 0.40 -1.3 2,200,160 - Pf6N2E2_1855 0.40 +0.7 2,200,160 - Pf6N2E2_1855 0.40 -0.1 2,200,160 - Pf6N2E2_1855 0.40 +0.7 2,200,418 - Pf6N2E2_1855 0.62 -0.5 2,200,511 - Pf6N2E2_1855 0.70 +1.2 2,200,511 - Pf6N2E2_1855 0.70 +0.8 2,200,553 + Pf6N2E2_1855 0.74 +0.3 2,200,553 + Pf6N2E2_1855 0.74 +0.5 2,200,554 - Pf6N2E2_1855 0.74 -0.2 2,200,637 - Pf6N2E2_1855 0.81 +0.4 2,200,640 + Pf6N2E2_1855 0.81 -1.3 2,200,640 + Pf6N2E2_1855 0.81 -0.1 2,200,640 + Pf6N2E2_1855 0.81 +0.3 2,200,640 + Pf6N2E2_1855 0.81 -3.3 2,200,640 + Pf6N2E2_1855 0.81 +0.8 2,200,641 - Pf6N2E2_1855 0.81 -0.9 2,200,641 - Pf6N2E2_1855 0.81 -1.1 2,200,641 - Pf6N2E2_1855 0.81 -0.1 2,200,747 + -0.8 2,200,748 - -0.1 2,200,748 - -0.9 2,200,749 + +0.7 2,200,749 + +0.9 2,200,749 + +0.4 2,200,750 - +0.7 2,200,750 - -0.1 2,200,750 - +0.5 2,200,777 + -1.5 2,200,777 + +0.7 2,200,778 - +0.2 2,200,804 + -0.2 2,200,805 - -0.3 2,200,895 - +1.0 2,200,910 - -0.3 2,201,071 + Pf6N2E2_1856 0.14 -2.2 2,201,072 - Pf6N2E2_1856 0.14 -2.4 2,201,072 - Pf6N2E2_1856 0.14 +0.9 2,201,076 - Pf6N2E2_1856 0.14 +1.1 2,201,144 - Pf6N2E2_1856 0.19 +0.6 2,201,168 + Pf6N2E2_1856 0.20 -0.6 2,201,170 + Pf6N2E2_1856 0.21 -0.3 2,201,170 + Pf6N2E2_1856 0.21 +1.3 2,201,170 + Pf6N2E2_1856 0.21 +0.6 2,201,170 + Pf6N2E2_1856 0.21 -1.1 2,201,171 - Pf6N2E2_1856 0.21 +0.3 2,201,171 - Pf6N2E2_1856 0.21 +0.9 2,201,171 - Pf6N2E2_1856 0.21 +1.1 2,201,171 - Pf6N2E2_1856 0.21 +1.3 2,201,171 - Pf6N2E2_1856 0.21 +2.4 2,201,213 + Pf6N2E2_1856 0.24 -0.2 2,201,213 + Pf6N2E2_1856 0.24 -1.5 2,201,216 - Pf6N2E2_1856 0.24 +0.8 2,201,222 - Pf6N2E2_1856 0.24 +0.1 2,201,327 - Pf6N2E2_1856 0.32 +1.3 2,201,398 - Pf6N2E2_1856 0.37 -0.3 2,201,624 - Pf6N2E2_1856 0.53 +0.5 2,201,676 + Pf6N2E2_1856 0.57 +0.9 2,201,677 - Pf6N2E2_1856 0.57 +2.7 2,201,800 - Pf6N2E2_1856 0.65 -1.3
Or see this region's nucleotide sequence