Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1849 and Pf6N2E2_1850 are separated by 228 nucleotides Pf6N2E2_1850 and Pf6N2E2_1851 are separated by 63 nucleotides
Pf6N2E2_1849 - FIG002188: hypothetical protein, at 2,188,255 to 2,189,619
_1849
Pf6N2E2_1850 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,189,848 to 2,191,995
_1850
Pf6N2E2_1851 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,192,059 to 2,193,267
_1851
Position (kb)
2189
2190
2191
2192 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2189.069 kb on + strand, within Pf6N2E2_1849 at 2189.069 kb on + strand, within Pf6N2E2_1849 at 2189.169 kb on - strand, within Pf6N2E2_1849 at 2189.206 kb on - strand, within Pf6N2E2_1849 at 2189.386 kb on - strand, within Pf6N2E2_1849 at 2189.429 kb on + strand, within Pf6N2E2_1849 at 2189.463 kb on - strand, within Pf6N2E2_1849 at 2189.636 kb on - strand at 2189.637 kb on + strand at 2189.638 kb on - strand at 2189.753 kb on + strand at 2189.785 kb on + strand at 2189.786 kb on - strand at 2189.786 kb on - strand at 2189.790 kb on + strand at 2189.791 kb on - strand at 2189.916 kb on - strand at 2189.916 kb on - strand at 2189.964 kb on - strand at 2190.056 kb on - strand at 2190.056 kb on - strand at 2190.161 kb on + strand, within Pf6N2E2_1850 at 2190.161 kb on + strand, within Pf6N2E2_1850 at 2190.206 kb on + strand, within Pf6N2E2_1850 at 2190.206 kb on + strand, within Pf6N2E2_1850 at 2190.206 kb on + strand, within Pf6N2E2_1850 at 2190.207 kb on - strand, within Pf6N2E2_1850 at 2190.207 kb on - strand, within Pf6N2E2_1850 at 2190.242 kb on + strand, within Pf6N2E2_1850 at 2190.243 kb on - strand, within Pf6N2E2_1850 at 2190.337 kb on - strand, within Pf6N2E2_1850 at 2190.406 kb on + strand, within Pf6N2E2_1850 at 2190.406 kb on + strand, within Pf6N2E2_1850 at 2190.406 kb on + strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.467 kb on + strand, within Pf6N2E2_1850 at 2190.825 kb on - strand, within Pf6N2E2_1850 at 2191.364 kb on - strand, within Pf6N2E2_1850 at 2191.635 kb on + strand, within Pf6N2E2_1850 at 2191.636 kb on - strand, within Pf6N2E2_1850 at 2191.870 kb on + strand at 2191.905 kb on - strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.996 kb on - strand at 2192.451 kb on + strand, within Pf6N2E2_1851 at 2192.505 kb on + strand, within Pf6N2E2_1851 at 2192.547 kb on - strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.551 kb on - strand, within Pf6N2E2_1851 at 2192.560 kb on + strand, within Pf6N2E2_1851 at 2192.561 kb on - strand, within Pf6N2E2_1851 at 2192.571 kb on - strand, within Pf6N2E2_1851 at 2192.581 kb on - strand, within Pf6N2E2_1851
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,189,069 + Pf6N2E2_1849 0.60 -0.9 2,189,069 + Pf6N2E2_1849 0.60 +0.2 2,189,169 - Pf6N2E2_1849 0.67 +1.0 2,189,206 - Pf6N2E2_1849 0.70 -0.1 2,189,386 - Pf6N2E2_1849 0.83 +0.9 2,189,429 + Pf6N2E2_1849 0.86 +0.8 2,189,463 - Pf6N2E2_1849 0.88 +0.2 2,189,636 - +0.7 2,189,637 + +0.4 2,189,638 - -1.6 2,189,753 + -1.5 2,189,785 + +0.0 2,189,786 - -0.6 2,189,786 - -1.6 2,189,790 + +1.4 2,189,791 - +1.0 2,189,916 - +0.1 2,189,916 - +0.4 2,189,964 - -0.1 2,190,056 - +1.0 2,190,056 - +0.6 2,190,161 + Pf6N2E2_1850 0.15 +0.1 2,190,161 + Pf6N2E2_1850 0.15 +2.1 2,190,206 + Pf6N2E2_1850 0.17 +0.5 2,190,206 + Pf6N2E2_1850 0.17 -0.9 2,190,206 + Pf6N2E2_1850 0.17 +0.8 2,190,207 - Pf6N2E2_1850 0.17 +0.0 2,190,207 - Pf6N2E2_1850 0.17 +0.6 2,190,242 + Pf6N2E2_1850 0.18 -0.8 2,190,243 - Pf6N2E2_1850 0.18 +1.3 2,190,337 - Pf6N2E2_1850 0.23 +0.6 2,190,406 + Pf6N2E2_1850 0.26 +0.8 2,190,406 + Pf6N2E2_1850 0.26 +0.6 2,190,406 + Pf6N2E2_1850 0.26 +0.7 2,190,407 - Pf6N2E2_1850 0.26 -0.6 2,190,407 - Pf6N2E2_1850 0.26 -0.3 2,190,407 - Pf6N2E2_1850 0.26 +0.1 2,190,407 - Pf6N2E2_1850 0.26 +0.6 2,190,407 - Pf6N2E2_1850 0.26 +1.0 2,190,407 - Pf6N2E2_1850 0.26 +0.7 2,190,467 + Pf6N2E2_1850 0.29 -0.9 2,190,825 - Pf6N2E2_1850 0.45 -0.8 2,191,364 - Pf6N2E2_1850 0.71 -0.6 2,191,635 + Pf6N2E2_1850 0.83 +1.9 2,191,636 - Pf6N2E2_1850 0.83 -1.6 2,191,870 + +2.3 2,191,905 - -0.1 2,191,995 + -1.6 2,191,995 + +0.7 2,191,995 + -1.0 2,191,995 + -0.0 2,191,995 + +0.7 2,191,996 - +2.5 2,192,451 + Pf6N2E2_1851 0.32 +0.4 2,192,505 + Pf6N2E2_1851 0.37 +0.7 2,192,547 - Pf6N2E2_1851 0.40 -0.6 2,192,550 + Pf6N2E2_1851 0.41 -0.5 2,192,550 + Pf6N2E2_1851 0.41 -1.1 2,192,550 + Pf6N2E2_1851 0.41 -0.3 2,192,551 - Pf6N2E2_1851 0.41 -0.9 2,192,560 + Pf6N2E2_1851 0.41 +0.3 2,192,561 - Pf6N2E2_1851 0.42 -2.5 2,192,571 - Pf6N2E2_1851 0.42 -0.4 2,192,581 - Pf6N2E2_1851 0.43 +0.0
Or see this region's nucleotide sequence