Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1494 and Pf6N2E2_1495 overlap by 11 nucleotides Pf6N2E2_1495 and Pf6N2E2_1496 overlap by 4 nucleotides Pf6N2E2_1496 and Pf6N2E2_1497 overlap by 4 nucleotides
Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761
_1494
Pf6N2E2_1495 - Glutamate transport membrane-spanning protein, at 1,748,751 to 1,749,485
_1495
Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693
_1496
Pf6N2E2_1497 - D-2-hydroxyglutarate dehydrogenase, at 1,750,690 to 1,752,114
_1497
Position (kb)
1749
1750
1751 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1748.665 kb on - strand, within Pf6N2E2_1494 at 1748.665 kb on - strand, within Pf6N2E2_1494 at 1748.716 kb on + strand at 1748.899 kb on + strand, within Pf6N2E2_1495 at 1748.899 kb on + strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1749.223 kb on + strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.409 kb on + strand, within Pf6N2E2_1495 at 1749.431 kb on - strand at 1749.592 kb on - strand at 1749.798 kb on + strand, within Pf6N2E2_1496 at 1749.969 kb on + strand, within Pf6N2E2_1496 at 1749.969 kb on + strand, within Pf6N2E2_1496 at 1749.970 kb on - strand, within Pf6N2E2_1496 at 1750.063 kb on + strand, within Pf6N2E2_1496 at 1750.071 kb on + strand, within Pf6N2E2_1496 at 1750.500 kb on + strand, within Pf6N2E2_1496 at 1750.501 kb on - strand, within Pf6N2E2_1496 at 1750.501 kb on - strand, within Pf6N2E2_1496 at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.684 kb on - strand at 1750.684 kb on - strand at 1750.684 kb on - strand at 1750.805 kb on - strand at 1750.880 kb on - strand, within Pf6N2E2_1497 at 1750.949 kb on + strand, within Pf6N2E2_1497 at 1750.949 kb on + strand, within Pf6N2E2_1497 at 1750.985 kb on + strand, within Pf6N2E2_1497 at 1751.085 kb on + strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.500 kb on - strand, within Pf6N2E2_1497
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,748,665 - Pf6N2E2_1494 0.88 +0.2 1,748,665 - Pf6N2E2_1494 0.88 -0.1 1,748,716 + +0.6 1,748,899 + Pf6N2E2_1495 0.20 -0.3 1,748,899 + Pf6N2E2_1495 0.20 +1.1 1,748,900 - Pf6N2E2_1495 0.20 +0.2 1,748,900 - Pf6N2E2_1495 0.20 +0.9 1,748,900 - Pf6N2E2_1495 0.20 +2.2 1,749,223 + Pf6N2E2_1495 0.64 -0.4 1,749,224 - Pf6N2E2_1495 0.64 +0.2 1,749,224 - Pf6N2E2_1495 0.64 -0.1 1,749,224 - Pf6N2E2_1495 0.64 +0.2 1,749,224 - Pf6N2E2_1495 0.64 -0.9 1,749,409 + Pf6N2E2_1495 0.90 +0.5 1,749,431 - +0.0 1,749,592 - -0.3 1,749,798 + Pf6N2E2_1496 0.26 -0.3 1,749,969 + Pf6N2E2_1496 0.40 -1.3 1,749,969 + Pf6N2E2_1496 0.40 -0.8 1,749,970 - Pf6N2E2_1496 0.40 -1.2 1,750,063 + Pf6N2E2_1496 0.48 +0.0 1,750,071 + Pf6N2E2_1496 0.49 -0.9 1,750,500 + Pf6N2E2_1496 0.84 -2.0 1,750,501 - Pf6N2E2_1496 0.84 +0.3 1,750,501 - Pf6N2E2_1496 0.84 +0.2 1,750,683 + -0.7 1,750,683 + -0.8 1,750,683 + +0.0 1,750,683 + +0.5 1,750,683 + +0.9 1,750,684 - +0.5 1,750,684 - +0.9 1,750,684 - -0.4 1,750,805 - +0.9 1,750,880 - Pf6N2E2_1497 0.13 +0.8 1,750,949 + Pf6N2E2_1497 0.18 +1.0 1,750,949 + Pf6N2E2_1497 0.18 +1.3 1,750,985 + Pf6N2E2_1497 0.21 -0.3 1,751,085 + Pf6N2E2_1497 0.28 +1.2 1,751,116 - Pf6N2E2_1497 0.30 +0.1 1,751,116 - Pf6N2E2_1497 0.30 +0.2 1,751,499 + Pf6N2E2_1497 0.57 -2.0 1,751,499 + Pf6N2E2_1497 0.57 -0.8 1,751,500 - Pf6N2E2_1497 0.57 -0.3
Or see this region's nucleotide sequence