Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1496

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1494 and Pf6N2E2_1495 overlap by 11 nucleotidesPf6N2E2_1495 and Pf6N2E2_1496 overlap by 4 nucleotidesPf6N2E2_1496 and Pf6N2E2_1497 overlap by 4 nucleotides Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761 _1494 Pf6N2E2_1495 - Glutamate transport membrane-spanning protein, at 1,748,751 to 1,749,485 _1495 Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693 _1496 Pf6N2E2_1497 - D-2-hydroxyglutarate dehydrogenase, at 1,750,690 to 1,752,114 _1497 Position (kb) 1749 1750 1751Strain fitness (log2 ratio) -2 -1 0 1 2at 1748.665 kb on - strand, within Pf6N2E2_1494at 1748.665 kb on - strand, within Pf6N2E2_1494at 1748.716 kb on + strandat 1748.899 kb on + strand, within Pf6N2E2_1495at 1748.899 kb on + strand, within Pf6N2E2_1495at 1748.900 kb on - strand, within Pf6N2E2_1495at 1748.900 kb on - strand, within Pf6N2E2_1495at 1748.900 kb on - strand, within Pf6N2E2_1495at 1749.223 kb on + strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.409 kb on + strand, within Pf6N2E2_1495at 1749.431 kb on - strandat 1749.592 kb on - strandat 1749.798 kb on + strand, within Pf6N2E2_1496at 1749.969 kb on + strand, within Pf6N2E2_1496at 1749.969 kb on + strand, within Pf6N2E2_1496at 1749.970 kb on - strand, within Pf6N2E2_1496at 1750.063 kb on + strand, within Pf6N2E2_1496at 1750.071 kb on + strand, within Pf6N2E2_1496at 1750.500 kb on + strand, within Pf6N2E2_1496at 1750.501 kb on - strand, within Pf6N2E2_1496at 1750.501 kb on - strand, within Pf6N2E2_1496at 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.684 kb on - strandat 1750.684 kb on - strandat 1750.684 kb on - strandat 1750.805 kb on - strandat 1750.880 kb on - strand, within Pf6N2E2_1497at 1750.949 kb on + strand, within Pf6N2E2_1497at 1750.949 kb on + strand, within Pf6N2E2_1497at 1750.985 kb on + strand, within Pf6N2E2_1497at 1751.085 kb on + strand, within Pf6N2E2_1497at 1751.116 kb on - strand, within Pf6N2E2_1497at 1751.116 kb on - strand, within Pf6N2E2_1497at 1751.499 kb on + strand, within Pf6N2E2_1497at 1751.499 kb on + strand, within Pf6N2E2_1497at 1751.500 kb on - strand, within Pf6N2E2_1497

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,748,665 - Pf6N2E2_1494 0.88 +0.2
1,748,665 - Pf6N2E2_1494 0.88 -0.1
1,748,716 + +0.6
1,748,899 + Pf6N2E2_1495 0.20 -0.3
1,748,899 + Pf6N2E2_1495 0.20 +1.1
1,748,900 - Pf6N2E2_1495 0.20 +0.2
1,748,900 - Pf6N2E2_1495 0.20 +0.9
1,748,900 - Pf6N2E2_1495 0.20 +2.2
1,749,223 + Pf6N2E2_1495 0.64 -0.4
1,749,224 - Pf6N2E2_1495 0.64 +0.2
1,749,224 - Pf6N2E2_1495 0.64 -0.1
1,749,224 - Pf6N2E2_1495 0.64 +0.2
1,749,224 - Pf6N2E2_1495 0.64 -0.9
1,749,409 + Pf6N2E2_1495 0.90 +0.5
1,749,431 - +0.0
1,749,592 - -0.3
1,749,798 + Pf6N2E2_1496 0.26 -0.3
1,749,969 + Pf6N2E2_1496 0.40 -1.3
1,749,969 + Pf6N2E2_1496 0.40 -0.8
1,749,970 - Pf6N2E2_1496 0.40 -1.2
1,750,063 + Pf6N2E2_1496 0.48 +0.0
1,750,071 + Pf6N2E2_1496 0.49 -0.9
1,750,500 + Pf6N2E2_1496 0.84 -2.0
1,750,501 - Pf6N2E2_1496 0.84 +0.3
1,750,501 - Pf6N2E2_1496 0.84 +0.2
1,750,683 + -0.7
1,750,683 + -0.8
1,750,683 + +0.0
1,750,683 + +0.5
1,750,683 + +0.9
1,750,684 - +0.5
1,750,684 - +0.9
1,750,684 - -0.4
1,750,805 - +0.9
1,750,880 - Pf6N2E2_1497 0.13 +0.8
1,750,949 + Pf6N2E2_1497 0.18 +1.0
1,750,949 + Pf6N2E2_1497 0.18 +1.3
1,750,985 + Pf6N2E2_1497 0.21 -0.3
1,751,085 + Pf6N2E2_1497 0.28 +1.2
1,751,116 - Pf6N2E2_1497 0.30 +0.1
1,751,116 - Pf6N2E2_1497 0.30 +0.2
1,751,499 + Pf6N2E2_1497 0.57 -2.0
1,751,499 + Pf6N2E2_1497 0.57 -0.8
1,751,500 - Pf6N2E2_1497 0.57 -0.3

Or see this region's nucleotide sequence