Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1267 and Pf6N2E2_1268 overlap by 33 nucleotides Pf6N2E2_1268 and Pf6N2E2_1269 are separated by 6 nucleotides
Pf6N2E2_1267 - Membrane protein, putative, at 1,493,505 to 1,494,284
_1267
Pf6N2E2_1268 - NADH pyrophosphatase (EC 3.6.1.22), at 1,494,252 to 1,495,082
_1268
Pf6N2E2_1269 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,495,089 to 1,495,901
_1269
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1493.321 kb on + strand at 1493.322 kb on - strand at 1493.322 kb on - strand at 1493.322 kb on - strand at 1493.404 kb on - strand at 1493.456 kb on - strand at 1493.489 kb on + strand at 1493.489 kb on + strand at 1493.489 kb on + strand at 1493.493 kb on + strand at 1493.493 kb on + strand at 1493.494 kb on - strand at 1493.499 kb on + strand at 1493.500 kb on - strand at 1493.500 kb on - strand at 1493.543 kb on + strand at 1493.543 kb on + strand at 1493.544 kb on - strand at 1493.712 kb on + strand, within Pf6N2E2_1267 at 1493.712 kb on + strand, within Pf6N2E2_1267 at 1493.712 kb on + strand, within Pf6N2E2_1267 at 1493.713 kb on - strand, within Pf6N2E2_1267 at 1493.713 kb on - strand, within Pf6N2E2_1267 at 1493.802 kb on + strand, within Pf6N2E2_1267 at 1493.802 kb on + strand, within Pf6N2E2_1267 at 1493.803 kb on - strand, within Pf6N2E2_1267 at 1493.803 kb on - strand, within Pf6N2E2_1267 at 1493.862 kb on + strand, within Pf6N2E2_1267 at 1493.862 kb on + strand, within Pf6N2E2_1267 at 1493.863 kb on - strand, within Pf6N2E2_1267 at 1493.863 kb on - strand, within Pf6N2E2_1267 at 1493.958 kb on + strand, within Pf6N2E2_1267 at 1493.959 kb on - strand, within Pf6N2E2_1267 at 1493.959 kb on - strand, within Pf6N2E2_1267 at 1494.056 kb on + strand, within Pf6N2E2_1267 at 1494.283 kb on - strand at 1494.298 kb on + strand at 1494.298 kb on + strand at 1494.298 kb on + strand at 1494.299 kb on - strand at 1494.299 kb on - strand at 1494.478 kb on + strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.635 kb on + strand, within Pf6N2E2_1268 at 1494.671 kb on - strand, within Pf6N2E2_1268 at 1494.697 kb on + strand, within Pf6N2E2_1268 at 1494.697 kb on + strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.805 kb on + strand, within Pf6N2E2_1268 at 1494.806 kb on - strand, within Pf6N2E2_1268 at 1494.806 kb on - strand, within Pf6N2E2_1268 at 1494.895 kb on + strand, within Pf6N2E2_1268 at 1494.896 kb on - strand, within Pf6N2E2_1268 at 1495.122 kb on - strand at 1495.222 kb on + strand at 1495.222 kb on + strand, within Pf6N2E2_1269 at 1495.222 kb on + strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.324 kb on + strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.383 kb on - strand, within Pf6N2E2_1269 at 1495.385 kb on - strand, within Pf6N2E2_1269 at 1495.656 kb on + strand, within Pf6N2E2_1269 at 1495.656 kb on + strand, within Pf6N2E2_1269 at 1495.657 kb on - strand, within Pf6N2E2_1269 at 1495.704 kb on + strand, within Pf6N2E2_1269 at 1495.867 kb on - strand at 1495.892 kb on - strand at 1495.892 kb on - strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.940 kb on - strand at 1495.940 kb on - strand at 1495.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,493,321 + -2.6 1,493,322 - -0.3 1,493,322 - +1.4 1,493,322 - -0.5 1,493,404 - -0.2 1,493,456 - +0.2 1,493,489 + +0.6 1,493,489 + -1.0 1,493,489 + -1.9 1,493,493 + +0.5 1,493,493 + +2.0 1,493,494 - -0.2 1,493,499 + +0.8 1,493,500 - +0.0 1,493,500 - +0.5 1,493,543 + -0.3 1,493,543 + -1.1 1,493,544 - -0.3 1,493,712 + Pf6N2E2_1267 0.27 +0.2 1,493,712 + Pf6N2E2_1267 0.27 +1.2 1,493,712 + Pf6N2E2_1267 0.27 -0.4 1,493,713 - Pf6N2E2_1267 0.27 +0.2 1,493,713 - Pf6N2E2_1267 0.27 -0.4 1,493,802 + Pf6N2E2_1267 0.38 +1.6 1,493,802 + Pf6N2E2_1267 0.38 +0.4 1,493,803 - Pf6N2E2_1267 0.38 +0.4 1,493,803 - Pf6N2E2_1267 0.38 -0.3 1,493,862 + Pf6N2E2_1267 0.46 +0.8 1,493,862 + Pf6N2E2_1267 0.46 -0.9 1,493,863 - Pf6N2E2_1267 0.46 +0.5 1,493,863 - Pf6N2E2_1267 0.46 +2.0 1,493,958 + Pf6N2E2_1267 0.58 -0.8 1,493,959 - Pf6N2E2_1267 0.58 +0.1 1,493,959 - Pf6N2E2_1267 0.58 +0.2 1,494,056 + Pf6N2E2_1267 0.71 +1.4 1,494,283 - +0.7 1,494,298 + -1.2 1,494,298 + +0.4 1,494,298 + -0.0 1,494,299 - +0.8 1,494,299 - -0.1 1,494,478 + Pf6N2E2_1268 0.27 -1.8 1,494,479 - Pf6N2E2_1268 0.27 +0.7 1,494,479 - Pf6N2E2_1268 0.27 -0.2 1,494,479 - Pf6N2E2_1268 0.27 +0.1 1,494,479 - Pf6N2E2_1268 0.27 +1.1 1,494,479 - Pf6N2E2_1268 0.27 +0.9 1,494,635 + Pf6N2E2_1268 0.46 -0.6 1,494,671 - Pf6N2E2_1268 0.50 +2.1 1,494,697 + Pf6N2E2_1268 0.54 +1.0 1,494,697 + Pf6N2E2_1268 0.54 -0.1 1,494,698 - Pf6N2E2_1268 0.54 +0.2 1,494,698 - Pf6N2E2_1268 0.54 -1.2 1,494,698 - Pf6N2E2_1268 0.54 +0.4 1,494,698 - Pf6N2E2_1268 0.54 +1.5 1,494,698 - Pf6N2E2_1268 0.54 -0.0 1,494,698 - Pf6N2E2_1268 0.54 -1.3 1,494,805 + Pf6N2E2_1268 0.67 -0.7 1,494,806 - Pf6N2E2_1268 0.67 +0.3 1,494,806 - Pf6N2E2_1268 0.67 -2.3 1,494,895 + Pf6N2E2_1268 0.77 -0.8 1,494,896 - Pf6N2E2_1268 0.77 -1.0 1,495,122 - -1.2 1,495,222 + +1.8 1,495,222 + Pf6N2E2_1269 0.16 -0.6 1,495,222 + Pf6N2E2_1269 0.16 +1.0 1,495,223 - Pf6N2E2_1269 0.16 -3.1 1,495,223 - Pf6N2E2_1269 0.16 +0.2 1,495,223 - Pf6N2E2_1269 0.16 -0.0 1,495,223 - Pf6N2E2_1269 0.16 -1.9 1,495,324 + Pf6N2E2_1269 0.29 +1.1 1,495,325 - Pf6N2E2_1269 0.29 +0.4 1,495,325 - Pf6N2E2_1269 0.29 -0.8 1,495,325 - Pf6N2E2_1269 0.29 +0.6 1,495,383 - Pf6N2E2_1269 0.36 -2.0 1,495,385 - Pf6N2E2_1269 0.36 +0.7 1,495,656 + Pf6N2E2_1269 0.70 +1.0 1,495,656 + Pf6N2E2_1269 0.70 +0.0 1,495,657 - Pf6N2E2_1269 0.70 -0.9 1,495,704 + Pf6N2E2_1269 0.76 -0.1 1,495,867 - -0.2 1,495,892 - -0.5 1,495,892 - -0.4 1,495,939 + +2.4 1,495,939 + -0.0 1,495,939 + +2.3 1,495,939 + -1.2 1,495,940 - -1.2 1,495,940 - -1.1 1,495,965 - -1.8
Or see this region's nucleotide sequence