Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1161 and Pf6N2E2_1162 are separated by 63 nucleotides Pf6N2E2_1162 and Pf6N2E2_1163 are separated by 155 nucleotides Pf6N2E2_1163 and Pf6N2E2_1164 are separated by 832 nucleotides
Pf6N2E2_1161 - FIG00961921: hypothetical protein, at 1,359,262 to 1,359,942
_1161
Pf6N2E2_1162 - Transmembrane amino acid efflux protein, at 1,360,006 to 1,360,635
_1162
Pf6N2E2_1163 - hypothetical protein, at 1,360,791 to 1,362,170
_1163
Pf6N2E2_1164 - hypothetical protein, at 1,363,003 to 1,364,856
_1164
Position (kb)
1360
1361
1362
1363 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1359.824 kb on - strand, within Pf6N2E2_1161 at 1359.824 kb on - strand, within Pf6N2E2_1161 at 1359.974 kb on + strand at 1360.071 kb on - strand, within Pf6N2E2_1162 at 1360.071 kb on - strand, within Pf6N2E2_1162 at 1360.071 kb on - strand, within Pf6N2E2_1162 at 1360.515 kb on + strand, within Pf6N2E2_1162 at 1360.613 kb on + strand at 1360.613 kb on + strand at 1360.614 kb on - strand at 1360.891 kb on - strand at 1360.952 kb on + strand, within Pf6N2E2_1163 at 1361.077 kb on - strand, within Pf6N2E2_1163 at 1361.406 kb on - strand, within Pf6N2E2_1163 at 1361.632 kb on + strand, within Pf6N2E2_1163 at 1361.680 kb on - strand, within Pf6N2E2_1163 at 1361.739 kb on + strand, within Pf6N2E2_1163 at 1361.740 kb on - strand, within Pf6N2E2_1163 at 1361.740 kb on - strand, within Pf6N2E2_1163 at 1362.156 kb on + strand at 1362.156 kb on + strand at 1362.157 kb on - strand at 1362.626 kb on + strand at 1362.626 kb on + strand at 1362.626 kb on + strand at 1362.627 kb on - strand at 1362.627 kb on - strand at 1362.649 kb on + strand at 1362.649 kb on + strand at 1362.661 kb on + strand at 1362.704 kb on + strand at 1362.704 kb on + strand at 1362.859 kb on + strand at 1362.859 kb on + strand at 1362.859 kb on + strand at 1362.859 kb on + strand at 1362.859 kb on + strand at 1362.859 kb on + strand at 1362.860 kb on - strand at 1362.860 kb on - strand at 1362.860 kb on - strand at 1362.860 kb on - strand at 1363.026 kb on + strand at 1363.150 kb on - strand at 1363.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,359,824 - Pf6N2E2_1161 0.83 +1.7 1,359,824 - Pf6N2E2_1161 0.83 -0.6 1,359,974 + -0.0 1,360,071 - Pf6N2E2_1162 0.10 +1.5 1,360,071 - Pf6N2E2_1162 0.10 -1.1 1,360,071 - Pf6N2E2_1162 0.10 +0.2 1,360,515 + Pf6N2E2_1162 0.81 -0.5 1,360,613 + -0.1 1,360,613 + -1.9 1,360,614 - -0.8 1,360,891 - -0.8 1,360,952 + Pf6N2E2_1163 0.12 +0.5 1,361,077 - Pf6N2E2_1163 0.21 -0.3 1,361,406 - Pf6N2E2_1163 0.45 -1.6 1,361,632 + Pf6N2E2_1163 0.61 +0.2 1,361,680 - Pf6N2E2_1163 0.64 -1.8 1,361,739 + Pf6N2E2_1163 0.69 -0.8 1,361,740 - Pf6N2E2_1163 0.69 -0.2 1,361,740 - Pf6N2E2_1163 0.69 -0.9 1,362,156 + -0.6 1,362,156 + +0.5 1,362,157 - +0.5 1,362,626 + +0.6 1,362,626 + +0.0 1,362,626 + -1.2 1,362,627 - +0.5 1,362,627 - +1.1 1,362,649 + -1.4 1,362,649 + -0.1 1,362,661 + -0.8 1,362,704 + +1.9 1,362,704 + -1.4 1,362,859 + -0.4 1,362,859 + -0.8 1,362,859 + -0.3 1,362,859 + -1.3 1,362,859 + -0.7 1,362,859 + +0.4 1,362,860 - +1.9 1,362,860 - -0.4 1,362,860 - +1.2 1,362,860 - +0.0 1,363,026 + +1.2 1,363,150 - -1.5 1,363,167 + -0.5
Or see this region's nucleotide sequence