Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1146

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1145 and Pf6N2E2_1146 are separated by 9 nucleotidesPf6N2E2_1146 and Pf6N2E2_1147 are separated by 13 nucleotides Pf6N2E2_1145 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 1,342,085 to 1,343,275 _1145 Pf6N2E2_1146 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data), at 1,343,285 to 1,344,412 _1146 Pf6N2E2_1147 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,344,426 to 1,345,199 _1147 Position (kb) 1343 1344 1345Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1343.270 kb on + strandat 1343.270 kb on + strandat 1343.271 kb on - strandat 1343.271 kb on - strandat 1343.271 kb on - strandat 1343.273 kb on + strandat 1343.281 kb on + strandat 1343.281 kb on + strandat 1343.282 kb on - strandat 1343.282 kb on - strandat 1343.282 kb on - strandat 1343.483 kb on + strand, within Pf6N2E2_1146at 1343.483 kb on + strand, within Pf6N2E2_1146at 1343.720 kb on + strand, within Pf6N2E2_1146at 1343.720 kb on + strand, within Pf6N2E2_1146at 1343.720 kb on + strand, within Pf6N2E2_1146at 1343.720 kb on + strand, within Pf6N2E2_1146at 1343.720 kb on + strand, within Pf6N2E2_1146at 1343.721 kb on - strand, within Pf6N2E2_1146at 1343.721 kb on - strand, within Pf6N2E2_1146at 1343.972 kb on + strand, within Pf6N2E2_1146at 1343.972 kb on + strand, within Pf6N2E2_1146at 1343.973 kb on - strand, within Pf6N2E2_1146at 1344.068 kb on + strand, within Pf6N2E2_1146at 1344.069 kb on - strand, within Pf6N2E2_1146at 1344.179 kb on + strand, within Pf6N2E2_1146at 1344.362 kb on + strandat 1344.362 kb on + strandat 1344.362 kb on + strandat 1344.363 kb on - strandat 1344.363 kb on - strandat 1344.363 kb on - strandat 1344.363 kb on - strandat 1344.363 kb on - strandat 1344.433 kb on - strandat 1344.433 kb on - strandat 1344.433 kb on - strandat 1344.660 kb on + strand, within Pf6N2E2_1147at 1344.661 kb on - strand, within Pf6N2E2_1147at 1345.031 kb on + strand, within Pf6N2E2_1147at 1345.032 kb on - strandat 1345.193 kb on + strandat 1345.193 kb on + strandat 1345.193 kb on + strandat 1345.194 kb on - strandat 1345.197 kb on + strandat 1345.197 kb on + strandat 1345.197 kb on + strandat 1345.197 kb on + strandat 1345.198 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,343,270 + +0.5
1,343,270 + -0.2
1,343,271 - -0.7
1,343,271 - -0.6
1,343,271 - +1.6
1,343,273 + +0.0
1,343,281 + -1.7
1,343,281 + -2.2
1,343,282 - -1.5
1,343,282 - -1.3
1,343,282 - -2.1
1,343,483 + Pf6N2E2_1146 0.18 -0.6
1,343,483 + Pf6N2E2_1146 0.18 -3.3
1,343,720 + Pf6N2E2_1146 0.39 +0.2
1,343,720 + Pf6N2E2_1146 0.39 -1.9
1,343,720 + Pf6N2E2_1146 0.39 -1.0
1,343,720 + Pf6N2E2_1146 0.39 -0.8
1,343,720 + Pf6N2E2_1146 0.39 -0.9
1,343,721 - Pf6N2E2_1146 0.39 -2.6
1,343,721 - Pf6N2E2_1146 0.39 +0.2
1,343,972 + Pf6N2E2_1146 0.61 -2.0
1,343,972 + Pf6N2E2_1146 0.61 -3.1
1,343,973 - Pf6N2E2_1146 0.61 -2.3
1,344,068 + Pf6N2E2_1146 0.69 -1.2
1,344,069 - Pf6N2E2_1146 0.70 -0.1
1,344,179 + Pf6N2E2_1146 0.79 -1.6
1,344,362 + -0.1
1,344,362 + -0.2
1,344,362 + -0.3
1,344,363 - -3.6
1,344,363 - -0.9
1,344,363 - -0.5
1,344,363 - -4.2
1,344,363 - -2.9
1,344,433 - -2.1
1,344,433 - -0.6
1,344,433 - -1.5
1,344,660 + Pf6N2E2_1147 0.30 -2.1
1,344,661 - Pf6N2E2_1147 0.30 -2.8
1,345,031 + Pf6N2E2_1147 0.78 -1.4
1,345,032 - -0.3
1,345,193 + -0.3
1,345,193 + -2.6
1,345,193 + -1.6
1,345,194 - -1.1
1,345,197 + +1.2
1,345,197 + +0.6
1,345,197 + -0.3
1,345,197 + +0.7
1,345,198 - +0.7

Or see this region's nucleotide sequence