Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1145 and Pf6N2E2_1146 are separated by 9 nucleotides Pf6N2E2_1146 and Pf6N2E2_1147 are separated by 13 nucleotides
Pf6N2E2_1145 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 1,342,085 to 1,343,275
_1145
Pf6N2E2_1146 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,343,285 to 1,344,412
_1146
Pf6N2E2_1147 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,344,426 to 1,345,199
_1147
Position (kb)
1343
1344
1345 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1343.270 kb on + strand at 1343.270 kb on + strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.273 kb on + strand at 1343.281 kb on + strand at 1343.281 kb on + strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.973 kb on - strand, within Pf6N2E2_1146 at 1344.068 kb on + strand, within Pf6N2E2_1146 at 1344.069 kb on - strand, within Pf6N2E2_1146 at 1344.179 kb on + strand, within Pf6N2E2_1146 at 1344.362 kb on + strand at 1344.362 kb on + strand at 1344.362 kb on + strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.660 kb on + strand, within Pf6N2E2_1147 at 1344.661 kb on - strand, within Pf6N2E2_1147 at 1345.031 kb on + strand, within Pf6N2E2_1147 at 1345.032 kb on - strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.194 kb on - strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.198 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,343,270 + +0.5 1,343,270 + -0.2 1,343,271 - -0.7 1,343,271 - -0.6 1,343,271 - +1.6 1,343,273 + +0.0 1,343,281 + -1.7 1,343,281 + -2.2 1,343,282 - -1.5 1,343,282 - -1.3 1,343,282 - -2.1 1,343,483 + Pf6N2E2_1146 0.18 -0.6 1,343,483 + Pf6N2E2_1146 0.18 -3.3 1,343,720 + Pf6N2E2_1146 0.39 +0.2 1,343,720 + Pf6N2E2_1146 0.39 -1.9 1,343,720 + Pf6N2E2_1146 0.39 -1.0 1,343,720 + Pf6N2E2_1146 0.39 -0.8 1,343,720 + Pf6N2E2_1146 0.39 -0.9 1,343,721 - Pf6N2E2_1146 0.39 -2.6 1,343,721 - Pf6N2E2_1146 0.39 +0.2 1,343,972 + Pf6N2E2_1146 0.61 -2.0 1,343,972 + Pf6N2E2_1146 0.61 -3.1 1,343,973 - Pf6N2E2_1146 0.61 -2.3 1,344,068 + Pf6N2E2_1146 0.69 -1.2 1,344,069 - Pf6N2E2_1146 0.70 -0.1 1,344,179 + Pf6N2E2_1146 0.79 -1.6 1,344,362 + -0.1 1,344,362 + -0.2 1,344,362 + -0.3 1,344,363 - -3.6 1,344,363 - -0.9 1,344,363 - -0.5 1,344,363 - -4.2 1,344,363 - -2.9 1,344,433 - -2.1 1,344,433 - -0.6 1,344,433 - -1.5 1,344,660 + Pf6N2E2_1147 0.30 -2.1 1,344,661 - Pf6N2E2_1147 0.30 -2.8 1,345,031 + Pf6N2E2_1147 0.78 -1.4 1,345,032 - -0.3 1,345,193 + -0.3 1,345,193 + -2.6 1,345,193 + -1.6 1,345,194 - -1.1 1,345,197 + +1.2 1,345,197 + +0.6 1,345,197 + -0.3 1,345,197 + +0.7 1,345,198 - +0.7
Or see this region's nucleotide sequence