Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_735

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_734 and Pf6N2E2_735 are separated by 277 nucleotidesPf6N2E2_735 and Pf6N2E2_736 are separated by 31 nucleotidesPf6N2E2_736 and Pf6N2E2_737 are separated by 5 nucleotidesPf6N2E2_737 and Pf6N2E2_738 are separated by 110 nucleotides Pf6N2E2_734 - Molybdenum cofactor biosynthesis protein MoaA, at 841,985 to 842,977 _734 Pf6N2E2_735 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 843,255 to 845,270 _735 Pf6N2E2_736 - hypothetical protein, at 845,302 to 845,463 _736 Pf6N2E2_737 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 845,469 to 846,155 _737 Pf6N2E2_738 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 846,266 to 847,885 _738 Position (kb) 843 844 845 846Strain fitness (log2 ratio) -2 -1 0 1 2at 842.517 kb on - strand, within Pf6N2E2_734at 842.684 kb on + strand, within Pf6N2E2_734at 842.684 kb on + strand, within Pf6N2E2_734at 842.684 kb on + strand, within Pf6N2E2_734at 842.685 kb on - strand, within Pf6N2E2_734at 842.975 kb on + strandat 842.981 kb on + strandat 842.981 kb on + strandat 842.981 kb on + strandat 842.981 kb on + strandat 843.088 kb on + strandat 843.088 kb on + strandat 843.088 kb on + strandat 843.088 kb on + strandat 843.089 kb on - strandat 843.128 kb on - strandat 843.128 kb on - strandat 843.233 kb on - strandat 843.234 kb on + strandat 843.234 kb on + strandat 843.235 kb on - strandat 843.235 kb on - strandat 843.235 kb on - strandat 843.238 kb on + strandat 843.238 kb on + strandat 843.238 kb on + strandat 843.238 kb on + strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.285 kb on - strandat 843.519 kb on + strand, within Pf6N2E2_735at 843.519 kb on + strand, within Pf6N2E2_735at 843.519 kb on + strand, within Pf6N2E2_735at 843.520 kb on - strand, within Pf6N2E2_735at 843.520 kb on - strand, within Pf6N2E2_735at 843.648 kb on + strand, within Pf6N2E2_735at 843.676 kb on + strand, within Pf6N2E2_735at 843.699 kb on + strand, within Pf6N2E2_735at 843.699 kb on + strand, within Pf6N2E2_735at 843.699 kb on + strand, within Pf6N2E2_735at 843.700 kb on - strand, within Pf6N2E2_735at 843.717 kb on + strand, within Pf6N2E2_735at 843.717 kb on + strand, within Pf6N2E2_735at 843.718 kb on - strand, within Pf6N2E2_735at 843.718 kb on - strand, within Pf6N2E2_735at 843.762 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.978 kb on + strand, within Pf6N2E2_735at 843.978 kb on + strand, within Pf6N2E2_735at 843.978 kb on + strand, within Pf6N2E2_735at 843.978 kb on + strand, within Pf6N2E2_735at 843.979 kb on - strand, within Pf6N2E2_735at 843.979 kb on - strand, within Pf6N2E2_735at 843.979 kb on - strand, within Pf6N2E2_735at 843.979 kb on - strand, within Pf6N2E2_735at 843.979 kb on - strand, within Pf6N2E2_735at 843.979 kb on - strandat 843.979 kb on - strand, within Pf6N2E2_735at 843.979 kb on - strand, within Pf6N2E2_735at 844.039 kb on - strand, within Pf6N2E2_735at 844.039 kb on - strand, within Pf6N2E2_735at 844.039 kb on - strand, within Pf6N2E2_735at 844.039 kb on - strand, within Pf6N2E2_735at 844.039 kb on - strand, within Pf6N2E2_735at 844.051 kb on + strand, within Pf6N2E2_735at 844.168 kb on - strand, within Pf6N2E2_735at 844.209 kb on + strand, within Pf6N2E2_735at 844.210 kb on - strand, within Pf6N2E2_735at 844.210 kb on - strand, within Pf6N2E2_735at 844.210 kb on - strand, within Pf6N2E2_735at 844.210 kb on - strand, within Pf6N2E2_735at 844.272 kb on + strand, within Pf6N2E2_735at 844.273 kb on - strand, within Pf6N2E2_735at 844.273 kb on - strand, within Pf6N2E2_735at 844.273 kb on - strand, within Pf6N2E2_735at 844.530 kb on + strand, within Pf6N2E2_735at 844.530 kb on + strand, within Pf6N2E2_735at 844.531 kb on - strand, within Pf6N2E2_735at 844.531 kb on - strand, within Pf6N2E2_735at 844.531 kb on - strand, within Pf6N2E2_735at 844.645 kb on - strand, within Pf6N2E2_735at 844.650 kb on + strand, within Pf6N2E2_735at 844.845 kb on + strand, within Pf6N2E2_735at 844.846 kb on - strand, within Pf6N2E2_735at 844.846 kb on - strand, within Pf6N2E2_735at 844.846 kb on - strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.951 kb on - strand, within Pf6N2E2_735at 844.953 kb on + strand, within Pf6N2E2_735at 844.954 kb on - strand, within Pf6N2E2_735at 844.954 kb on - strand, within Pf6N2E2_735at 845.014 kb on + strand, within Pf6N2E2_735at 845.014 kb on + strand, within Pf6N2E2_735at 845.015 kb on - strand, within Pf6N2E2_735at 845.037 kb on + strand, within Pf6N2E2_735at 845.037 kb on + strand, within Pf6N2E2_735at 845.038 kb on - strand, within Pf6N2E2_735at 845.038 kb on - strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.187 kb on + strandat 845.188 kb on - strandat 845.403 kb on + strand, within Pf6N2E2_736at 845.403 kb on + strand, within Pf6N2E2_736at 845.403 kb on + strand, within Pf6N2E2_736at 845.403 kb on + strand, within Pf6N2E2_736at 845.404 kb on - strand, within Pf6N2E2_736at 845.404 kb on - strand, within Pf6N2E2_736at 845.533 kb on + strandat 845.931 kb on + strand, within Pf6N2E2_737at 845.932 kb on - strand, within Pf6N2E2_737at 846.145 kb on + strandat 846.146 kb on - strandat 846.243 kb on + strandat 846.243 kb on + strandat 846.244 kb on - strandat 846.244 kb on - strandat 846.244 kb on - strandat 846.265 kb on + strandat 846.265 kb on + strandat 846.266 kb on - strandat 846.266 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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842,517 - Pf6N2E2_734 0.54 +0.5
842,684 + Pf6N2E2_734 0.70 +0.1
842,684 + Pf6N2E2_734 0.70 -0.1
842,684 + Pf6N2E2_734 0.70 -0.1
842,685 - Pf6N2E2_734 0.70 +0.3
842,975 + -0.8
842,981 + +0.2
842,981 + -0.2
842,981 + -0.4
842,981 + -0.0
843,088 + +2.0
843,088 + -0.0
843,088 + +0.7
843,088 + +0.8
843,089 - +1.1
843,128 - +1.0
843,128 - +0.9
843,233 - +0.0
843,234 + +0.3
843,234 + -0.5
843,235 - +0.2
843,235 - -1.4
843,235 - +0.5
843,238 + -1.5
843,238 + -0.1
843,238 + +0.5
843,238 + +1.1
843,239 - +0.3
843,239 - -0.1
843,239 - +0.0
843,239 - -0.1
843,239 - +0.1
843,239 - -1.1
843,285 - +0.4
843,519 + Pf6N2E2_735 0.13 -0.3
843,519 + Pf6N2E2_735 0.13 +1.2
843,519 + Pf6N2E2_735 0.13 +0.5
843,520 - Pf6N2E2_735 0.13 +0.8
843,520 - Pf6N2E2_735 0.13 -0.0
843,648 + Pf6N2E2_735 0.19 -1.7
843,676 + Pf6N2E2_735 0.21 -1.5
843,699 + Pf6N2E2_735 0.22 +0.5
843,699 + Pf6N2E2_735 0.22 +0.0
843,699 + Pf6N2E2_735 0.22 +0.9
843,700 - Pf6N2E2_735 0.22 -0.0
843,717 + Pf6N2E2_735 0.23 -0.2
843,717 + Pf6N2E2_735 0.23 -0.7
843,718 - Pf6N2E2_735 0.23 -0.0
843,718 - Pf6N2E2_735 0.23 -0.5
843,762 - Pf6N2E2_735 0.25 +0.4
843,967 - Pf6N2E2_735 0.35 +0.6
843,967 - Pf6N2E2_735 0.35 +0.1
843,967 - Pf6N2E2_735 0.35 +0.2
843,967 - Pf6N2E2_735 0.35 -0.5
843,967 - Pf6N2E2_735 0.35 -2.1
843,978 + Pf6N2E2_735 0.36 -0.2
843,978 + Pf6N2E2_735 0.36 -1.0
843,978 + Pf6N2E2_735 0.36 -2.3
843,978 + Pf6N2E2_735 0.36 -0.1
843,979 - Pf6N2E2_735 0.36 -1.3
843,979 - Pf6N2E2_735 0.36 -0.1
843,979 - Pf6N2E2_735 0.36 -0.4
843,979 - Pf6N2E2_735 0.36 +0.3
843,979 - Pf6N2E2_735 0.36 -1.3
843,979 - +0.5
843,979 - Pf6N2E2_735 0.36 +0.2
843,979 - Pf6N2E2_735 0.36 -0.3
844,039 - Pf6N2E2_735 0.39 -0.2
844,039 - Pf6N2E2_735 0.39 +2.5
844,039 - Pf6N2E2_735 0.39 -0.1
844,039 - Pf6N2E2_735 0.39 -0.6
844,039 - Pf6N2E2_735 0.39 +0.2
844,051 + Pf6N2E2_735 0.39 -1.1
844,168 - Pf6N2E2_735 0.45 +0.7
844,209 + Pf6N2E2_735 0.47 -1.5
844,210 - Pf6N2E2_735 0.47 +0.1
844,210 - Pf6N2E2_735 0.47 +0.5
844,210 - Pf6N2E2_735 0.47 +0.7
844,210 - Pf6N2E2_735 0.47 -0.1
844,272 + Pf6N2E2_735 0.50 -1.5
844,273 - Pf6N2E2_735 0.50 -0.1
844,273 - Pf6N2E2_735 0.50 +0.1
844,273 - Pf6N2E2_735 0.50 -0.6
844,530 + Pf6N2E2_735 0.63 +0.9
844,530 + Pf6N2E2_735 0.63 +0.3
844,531 - Pf6N2E2_735 0.63 -0.4
844,531 - Pf6N2E2_735 0.63 -0.7
844,531 - Pf6N2E2_735 0.63 -0.9
844,645 - Pf6N2E2_735 0.69 -0.8
844,650 + Pf6N2E2_735 0.69 +0.0
844,845 + Pf6N2E2_735 0.79 -0.1
844,846 - Pf6N2E2_735 0.79 -0.9
844,846 - Pf6N2E2_735 0.79 +1.0
844,846 - Pf6N2E2_735 0.79 +0.2
844,950 + Pf6N2E2_735 0.84 +1.8
844,950 + Pf6N2E2_735 0.84 -1.1
844,950 + Pf6N2E2_735 0.84 -0.8
844,950 + Pf6N2E2_735 0.84 -0.7
844,950 + Pf6N2E2_735 0.84 -0.5
844,951 - Pf6N2E2_735 0.84 -0.2
844,953 + Pf6N2E2_735 0.84 -0.2
844,954 - Pf6N2E2_735 0.84 -0.3
844,954 - Pf6N2E2_735 0.84 +0.7
845,014 + Pf6N2E2_735 0.87 -2.1
845,014 + Pf6N2E2_735 0.87 -0.1
845,015 - Pf6N2E2_735 0.87 -0.3
845,037 + Pf6N2E2_735 0.88 -0.4
845,037 + Pf6N2E2_735 0.88 -1.1
845,038 - Pf6N2E2_735 0.88 +1.1
845,038 - Pf6N2E2_735 0.88 +0.4
845,064 + Pf6N2E2_735 0.90 -0.8
845,064 + Pf6N2E2_735 0.90 -1.0
845,064 + Pf6N2E2_735 0.90 +0.2
845,064 + Pf6N2E2_735 0.90 -1.3
845,064 + Pf6N2E2_735 0.90 -0.2
845,065 - Pf6N2E2_735 0.90 -1.4
845,065 - Pf6N2E2_735 0.90 +0.4
845,065 - Pf6N2E2_735 0.90 -0.1
845,065 - Pf6N2E2_735 0.90 +0.3
845,187 + -0.9
845,188 - -0.6
845,403 + Pf6N2E2_736 0.62 +0.5
845,403 + Pf6N2E2_736 0.62 -0.8
845,403 + Pf6N2E2_736 0.62 -0.6
845,403 + Pf6N2E2_736 0.62 +1.7
845,404 - Pf6N2E2_736 0.63 +0.1
845,404 - Pf6N2E2_736 0.63 -1.9
845,533 + +0.8
845,931 + Pf6N2E2_737 0.67 +0.2
845,932 - Pf6N2E2_737 0.67 -0.0
846,145 + -0.1
846,146 - -0.4
846,243 + -0.8
846,243 + +1.8
846,244 - -1.2
846,244 - -0.4
846,244 - -0.4
846,265 + +1.2
846,265 + -0.6
846,266 - +0.2
846,266 - -0.1

Or see this region's nucleotide sequence