Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6088 and Pf6N2E2_6089 are separated by 72 nucleotides Pf6N2E2_6089 and Pf6N2E2_6090 are separated by 99 nucleotides Pf6N2E2_6090 and Pf6N2E2_6091 overlap by 32 nucleotides Pf6N2E2_6091 and Pf6N2E2_6092 are separated by 28 nucleotides
Pf6N2E2_6088 - hypothetical protein, at 6,912,888 to 6,913,145
_6088
Pf6N2E2_6089 - ThiJ/PfpI family protein, at 6,913,218 to 6,913,904
_6089
Pf6N2E2_6090 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 6,914,004 to 6,914,996
_6090
Pf6N2E2_6091 - Epoxide hydrolase (EC 3.3.2.9), at 6,914,965 to 6,915,867
_6091
Pf6N2E2_6092 - hypothetical protein, at 6,915,896 to 6,916,273
_6092
Position (kb)
6914
6915 Strain fitness (log2 ratio)
-1
0
1 at 6913.014 kb on + strand, within Pf6N2E2_6088 at 6913.015 kb on - strand, within Pf6N2E2_6088 at 6913.015 kb on - strand, within Pf6N2E2_6088 at 6913.027 kb on - strand, within Pf6N2E2_6088 at 6913.054 kb on - strand, within Pf6N2E2_6088 at 6913.364 kb on - strand, within Pf6N2E2_6089 at 6913.534 kb on + strand, within Pf6N2E2_6089 at 6913.535 kb on - strand, within Pf6N2E2_6089 at 6913.618 kb on + strand, within Pf6N2E2_6089 at 6913.618 kb on + strand, within Pf6N2E2_6089 at 6913.619 kb on - strand, within Pf6N2E2_6089 at 6913.619 kb on - strand, within Pf6N2E2_6089 at 6913.619 kb on - strand, within Pf6N2E2_6089 at 6913.833 kb on + strand, within Pf6N2E2_6089 at 6913.834 kb on - strand, within Pf6N2E2_6089 at 6913.840 kb on + strand at 6913.841 kb on - strand at 6913.953 kb on - strand at 6914.003 kb on + strand at 6914.134 kb on - strand, within Pf6N2E2_6090 at 6914.259 kb on + strand, within Pf6N2E2_6090 at 6914.735 kb on + strand, within Pf6N2E2_6090 at 6914.946 kb on + strand at 6915.059 kb on + strand, within Pf6N2E2_6091 at 6915.059 kb on + strand, within Pf6N2E2_6091 at 6915.212 kb on + strand, within Pf6N2E2_6091 at 6915.212 kb on + strand, within Pf6N2E2_6091 at 6915.212 kb on + strand, within Pf6N2E2_6091 at 6915.212 kb on + strand, within Pf6N2E2_6091 at 6915.213 kb on - strand, within Pf6N2E2_6091 at 6915.213 kb on - strand, within Pf6N2E2_6091 at 6915.230 kb on + strand, within Pf6N2E2_6091 at 6915.231 kb on - strand, within Pf6N2E2_6091 at 6915.398 kb on + strand, within Pf6N2E2_6091 at 6915.398 kb on + strand, within Pf6N2E2_6091 at 6915.398 kb on + strand, within Pf6N2E2_6091 at 6915.398 kb on + strand, within Pf6N2E2_6091 at 6915.399 kb on - strand, within Pf6N2E2_6091 at 6915.399 kb on - strand, within Pf6N2E2_6091 at 6915.401 kb on + strand, within Pf6N2E2_6091 at 6915.402 kb on - strand, within Pf6N2E2_6091 at 6915.473 kb on + strand, within Pf6N2E2_6091 at 6915.918 kb on + strand at 6915.918 kb on + strand at 6915.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 6,913,014 + Pf6N2E2_6088 0.49 -0.4 6,913,015 - Pf6N2E2_6088 0.49 +0.8 6,913,015 - Pf6N2E2_6088 0.49 +0.4 6,913,027 - Pf6N2E2_6088 0.54 -0.3 6,913,054 - Pf6N2E2_6088 0.64 +0.4 6,913,364 - Pf6N2E2_6089 0.21 -0.2 6,913,534 + Pf6N2E2_6089 0.46 +0.6 6,913,535 - Pf6N2E2_6089 0.46 -0.1 6,913,618 + Pf6N2E2_6089 0.58 +0.1 6,913,618 + Pf6N2E2_6089 0.58 -0.7 6,913,619 - Pf6N2E2_6089 0.58 +0.3 6,913,619 - Pf6N2E2_6089 0.58 -0.1 6,913,619 - Pf6N2E2_6089 0.58 +0.3 6,913,833 + Pf6N2E2_6089 0.90 -0.0 6,913,834 - Pf6N2E2_6089 0.90 -0.8 6,913,840 + +0.3 6,913,841 - +1.6 6,913,953 - +0.3 6,914,003 + -0.2 6,914,134 - Pf6N2E2_6090 0.13 -1.1 6,914,259 + Pf6N2E2_6090 0.26 -1.0 6,914,735 + Pf6N2E2_6090 0.74 -1.3 6,914,946 + +0.6 6,915,059 + Pf6N2E2_6091 0.10 +1.1 6,915,059 + Pf6N2E2_6091 0.10 +0.6 6,915,212 + Pf6N2E2_6091 0.27 -0.6 6,915,212 + Pf6N2E2_6091 0.27 -1.7 6,915,212 + Pf6N2E2_6091 0.27 +1.0 6,915,212 + Pf6N2E2_6091 0.27 -0.1 6,915,213 - Pf6N2E2_6091 0.27 +0.1 6,915,213 - Pf6N2E2_6091 0.27 -0.2 6,915,230 + Pf6N2E2_6091 0.29 -1.0 6,915,231 - Pf6N2E2_6091 0.29 -0.2 6,915,398 + Pf6N2E2_6091 0.48 -0.4 6,915,398 + Pf6N2E2_6091 0.48 +0.5 6,915,398 + Pf6N2E2_6091 0.48 +0.0 6,915,398 + Pf6N2E2_6091 0.48 +0.4 6,915,399 - Pf6N2E2_6091 0.48 +0.6 6,915,399 - Pf6N2E2_6091 0.48 -0.7 6,915,401 + Pf6N2E2_6091 0.48 +0.7 6,915,402 - Pf6N2E2_6091 0.48 +0.0 6,915,473 + Pf6N2E2_6091 0.56 +1.5 6,915,918 + +1.7 6,915,918 + +0.1 6,915,919 - -1.5
Or see this region's nucleotide sequence