Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4358 and Pf6N2E2_4359 are separated by 51 nucleotides Pf6N2E2_4359 and Pf6N2E2_4360 are separated by 55 nucleotides Pf6N2E2_4360 and Pf6N2E2_4361 are separated by 24 nucleotides
Pf6N2E2_4358 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,088,800 to 5,089,699
_4358
Pf6N2E2_4359 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,089,751 to 5,090,491
_4359
Pf6N2E2_4360 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 5,090,547 to 5,090,927
_4360
Pf6N2E2_4361 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 5,090,952 to 5,093,057
_4361
Position (kb)
5090
5091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5089.724 kb on + strand at 5089.725 kb on - strand at 5089.730 kb on + strand at 5089.730 kb on + strand at 5089.731 kb on - strand at 5089.731 kb on - strand at 5089.731 kb on - strand at 5090.067 kb on + strand, within Pf6N2E2_4359 at 5090.171 kb on + strand, within Pf6N2E2_4359 at 5090.172 kb on - strand, within Pf6N2E2_4359 at 5090.214 kb on + strand, within Pf6N2E2_4359 at 5090.286 kb on + strand, within Pf6N2E2_4359 at 5090.545 kb on + strand at 5090.545 kb on + strand at 5090.545 kb on + strand at 5090.546 kb on - strand at 5090.546 kb on - strand at 5090.548 kb on + strand at 5090.548 kb on + strand at 5090.549 kb on - strand at 5090.549 kb on - strand at 5090.549 kb on - strand at 5090.629 kb on + strand, within Pf6N2E2_4360 at 5090.629 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.842 kb on + strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.843 kb on - strand, within Pf6N2E2_4360 at 5090.875 kb on + strand, within Pf6N2E2_4360 at 5090.875 kb on + strand, within Pf6N2E2_4360 at 5090.875 kb on + strand, within Pf6N2E2_4360 at 5090.875 kb on + strand, within Pf6N2E2_4360 at 5090.875 kb on + strand, within Pf6N2E2_4360 at 5090.876 kb on - strand, within Pf6N2E2_4360 at 5090.876 kb on - strand, within Pf6N2E2_4360 at 5090.876 kb on - strand, within Pf6N2E2_4360 at 5090.876 kb on - strand, within Pf6N2E2_4360 at 5090.880 kb on - strand, within Pf6N2E2_4360 at 5090.880 kb on - strand, within Pf6N2E2_4360 at 5090.880 kb on - strand, within Pf6N2E2_4360 at 5090.880 kb on - strand, within Pf6N2E2_4360 at 5090.880 kb on - strand, within Pf6N2E2_4360 at 5090.913 kb on - strand at 5090.913 kb on - strand at 5090.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 5,089,724 + -1.7 5,089,725 - +0.6 5,089,730 + +0.5 5,089,730 + +0.8 5,089,731 - +1.4 5,089,731 - +0.5 5,089,731 - -0.3 5,090,067 + Pf6N2E2_4359 0.43 +0.5 5,090,171 + Pf6N2E2_4359 0.57 +0.4 5,090,172 - Pf6N2E2_4359 0.57 -0.2 5,090,214 + Pf6N2E2_4359 0.62 -1.5 5,090,286 + Pf6N2E2_4359 0.72 +1.3 5,090,545 + -0.6 5,090,545 + +0.3 5,090,545 + +0.8 5,090,546 - -0.1 5,090,546 - +0.8 5,090,548 + +0.5 5,090,548 + -0.7 5,090,549 - +0.6 5,090,549 - +1.6 5,090,549 - +0.1 5,090,629 + Pf6N2E2_4360 0.22 -0.8 5,090,629 + Pf6N2E2_4360 0.22 -0.8 5,090,644 + Pf6N2E2_4360 0.25 +2.0 5,090,644 + Pf6N2E2_4360 0.25 -2.9 5,090,644 + Pf6N2E2_4360 0.25 -0.1 5,090,644 + Pf6N2E2_4360 0.25 -2.0 5,090,644 + Pf6N2E2_4360 0.25 -0.2 5,090,645 - Pf6N2E2_4360 0.26 -0.9 5,090,645 - Pf6N2E2_4360 0.26 -1.2 5,090,645 - Pf6N2E2_4360 0.26 -1.4 5,090,645 - Pf6N2E2_4360 0.26 -0.1 5,090,842 + Pf6N2E2_4360 0.77 -1.1 5,090,842 + Pf6N2E2_4360 0.77 -1.5 5,090,842 + Pf6N2E2_4360 0.77 -0.8 5,090,842 + Pf6N2E2_4360 0.77 -1.7 5,090,842 + Pf6N2E2_4360 0.77 +0.4 5,090,842 + Pf6N2E2_4360 0.77 -0.1 5,090,842 + Pf6N2E2_4360 0.77 -2.1 5,090,842 + Pf6N2E2_4360 0.77 -0.2 5,090,843 - Pf6N2E2_4360 0.78 -0.3 5,090,843 - Pf6N2E2_4360 0.78 -1.6 5,090,843 - Pf6N2E2_4360 0.78 -0.1 5,090,843 - Pf6N2E2_4360 0.78 -0.5 5,090,843 - Pf6N2E2_4360 0.78 -2.2 5,090,843 - Pf6N2E2_4360 0.78 -0.9 5,090,843 - Pf6N2E2_4360 0.78 -2.0 5,090,843 - Pf6N2E2_4360 0.78 -2.2 5,090,843 - Pf6N2E2_4360 0.78 -1.5 5,090,843 - Pf6N2E2_4360 0.78 -0.8 5,090,843 - Pf6N2E2_4360 0.78 +0.0 5,090,843 - Pf6N2E2_4360 0.78 -1.1 5,090,875 + Pf6N2E2_4360 0.86 -0.5 5,090,875 + Pf6N2E2_4360 0.86 -0.4 5,090,875 + Pf6N2E2_4360 0.86 -1.0 5,090,875 + Pf6N2E2_4360 0.86 -0.2 5,090,875 + Pf6N2E2_4360 0.86 -0.7 5,090,876 - Pf6N2E2_4360 0.86 -1.1 5,090,876 - Pf6N2E2_4360 0.86 -1.0 5,090,876 - Pf6N2E2_4360 0.86 +0.2 5,090,876 - Pf6N2E2_4360 0.86 -0.6 5,090,880 - Pf6N2E2_4360 0.87 -1.3 5,090,880 - Pf6N2E2_4360 0.87 -1.9 5,090,880 - Pf6N2E2_4360 0.87 +1.6 5,090,880 - Pf6N2E2_4360 0.87 +1.1 5,090,880 - Pf6N2E2_4360 0.87 -0.6 5,090,913 - -2.2 5,090,913 - -1.4 5,090,954 - +0.2
Or see this region's nucleotide sequence