Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4360

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4358 and Pf6N2E2_4359 are separated by 51 nucleotidesPf6N2E2_4359 and Pf6N2E2_4360 are separated by 55 nucleotidesPf6N2E2_4360 and Pf6N2E2_4361 are separated by 24 nucleotides Pf6N2E2_4358 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,088,800 to 5,089,699 _4358 Pf6N2E2_4359 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,089,751 to 5,090,491 _4359 Pf6N2E2_4360 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 5,090,547 to 5,090,927 _4360 Pf6N2E2_4361 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 5,090,952 to 5,093,057 _4361 Position (kb) 5090 5091Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5089.724 kb on + strandat 5089.725 kb on - strandat 5089.730 kb on + strandat 5089.730 kb on + strandat 5089.731 kb on - strandat 5089.731 kb on - strandat 5089.731 kb on - strandat 5090.067 kb on + strand, within Pf6N2E2_4359at 5090.171 kb on + strand, within Pf6N2E2_4359at 5090.172 kb on - strand, within Pf6N2E2_4359at 5090.214 kb on + strand, within Pf6N2E2_4359at 5090.286 kb on + strand, within Pf6N2E2_4359at 5090.545 kb on + strandat 5090.545 kb on + strandat 5090.545 kb on + strandat 5090.546 kb on - strandat 5090.546 kb on - strandat 5090.548 kb on + strandat 5090.548 kb on + strandat 5090.549 kb on - strandat 5090.549 kb on - strandat 5090.549 kb on - strandat 5090.629 kb on + strand, within Pf6N2E2_4360at 5090.629 kb on + strand, within Pf6N2E2_4360at 5090.644 kb on + strand, within Pf6N2E2_4360at 5090.644 kb on + strand, within Pf6N2E2_4360at 5090.644 kb on + strand, within Pf6N2E2_4360at 5090.644 kb on + strand, within Pf6N2E2_4360at 5090.644 kb on + strand, within Pf6N2E2_4360at 5090.645 kb on - strand, within Pf6N2E2_4360at 5090.645 kb on - strand, within Pf6N2E2_4360at 5090.645 kb on - strand, within Pf6N2E2_4360at 5090.645 kb on - strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.842 kb on + strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.843 kb on - strand, within Pf6N2E2_4360at 5090.875 kb on + strand, within Pf6N2E2_4360at 5090.875 kb on + strand, within Pf6N2E2_4360at 5090.875 kb on + strand, within Pf6N2E2_4360at 5090.875 kb on + strand, within Pf6N2E2_4360at 5090.875 kb on + strand, within Pf6N2E2_4360at 5090.876 kb on - strand, within Pf6N2E2_4360at 5090.876 kb on - strand, within Pf6N2E2_4360at 5090.876 kb on - strand, within Pf6N2E2_4360at 5090.876 kb on - strand, within Pf6N2E2_4360at 5090.880 kb on - strand, within Pf6N2E2_4360at 5090.880 kb on - strand, within Pf6N2E2_4360at 5090.880 kb on - strand, within Pf6N2E2_4360at 5090.880 kb on - strand, within Pf6N2E2_4360at 5090.880 kb on - strand, within Pf6N2E2_4360at 5090.913 kb on - strandat 5090.913 kb on - strandat 5090.954 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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5,089,724 + -1.7
5,089,725 - +0.6
5,089,730 + +0.5
5,089,730 + +0.8
5,089,731 - +1.4
5,089,731 - +0.5
5,089,731 - -0.3
5,090,067 + Pf6N2E2_4359 0.43 +0.5
5,090,171 + Pf6N2E2_4359 0.57 +0.4
5,090,172 - Pf6N2E2_4359 0.57 -0.2
5,090,214 + Pf6N2E2_4359 0.62 -1.5
5,090,286 + Pf6N2E2_4359 0.72 +1.3
5,090,545 + -0.6
5,090,545 + +0.3
5,090,545 + +0.8
5,090,546 - -0.1
5,090,546 - +0.8
5,090,548 + +0.5
5,090,548 + -0.7
5,090,549 - +0.6
5,090,549 - +1.6
5,090,549 - +0.1
5,090,629 + Pf6N2E2_4360 0.22 -0.8
5,090,629 + Pf6N2E2_4360 0.22 -0.8
5,090,644 + Pf6N2E2_4360 0.25 +2.0
5,090,644 + Pf6N2E2_4360 0.25 -2.9
5,090,644 + Pf6N2E2_4360 0.25 -0.1
5,090,644 + Pf6N2E2_4360 0.25 -2.0
5,090,644 + Pf6N2E2_4360 0.25 -0.2
5,090,645 - Pf6N2E2_4360 0.26 -0.9
5,090,645 - Pf6N2E2_4360 0.26 -1.2
5,090,645 - Pf6N2E2_4360 0.26 -1.4
5,090,645 - Pf6N2E2_4360 0.26 -0.1
5,090,842 + Pf6N2E2_4360 0.77 -1.1
5,090,842 + Pf6N2E2_4360 0.77 -1.5
5,090,842 + Pf6N2E2_4360 0.77 -0.8
5,090,842 + Pf6N2E2_4360 0.77 -1.7
5,090,842 + Pf6N2E2_4360 0.77 +0.4
5,090,842 + Pf6N2E2_4360 0.77 -0.1
5,090,842 + Pf6N2E2_4360 0.77 -2.1
5,090,842 + Pf6N2E2_4360 0.77 -0.2
5,090,843 - Pf6N2E2_4360 0.78 -0.3
5,090,843 - Pf6N2E2_4360 0.78 -1.6
5,090,843 - Pf6N2E2_4360 0.78 -0.1
5,090,843 - Pf6N2E2_4360 0.78 -0.5
5,090,843 - Pf6N2E2_4360 0.78 -2.2
5,090,843 - Pf6N2E2_4360 0.78 -0.9
5,090,843 - Pf6N2E2_4360 0.78 -2.0
5,090,843 - Pf6N2E2_4360 0.78 -2.2
5,090,843 - Pf6N2E2_4360 0.78 -1.5
5,090,843 - Pf6N2E2_4360 0.78 -0.8
5,090,843 - Pf6N2E2_4360 0.78 +0.0
5,090,843 - Pf6N2E2_4360 0.78 -1.1
5,090,875 + Pf6N2E2_4360 0.86 -0.5
5,090,875 + Pf6N2E2_4360 0.86 -0.4
5,090,875 + Pf6N2E2_4360 0.86 -1.0
5,090,875 + Pf6N2E2_4360 0.86 -0.2
5,090,875 + Pf6N2E2_4360 0.86 -0.7
5,090,876 - Pf6N2E2_4360 0.86 -1.1
5,090,876 - Pf6N2E2_4360 0.86 -1.0
5,090,876 - Pf6N2E2_4360 0.86 +0.2
5,090,876 - Pf6N2E2_4360 0.86 -0.6
5,090,880 - Pf6N2E2_4360 0.87 -1.3
5,090,880 - Pf6N2E2_4360 0.87 -1.9
5,090,880 - Pf6N2E2_4360 0.87 +1.6
5,090,880 - Pf6N2E2_4360 0.87 +1.1
5,090,880 - Pf6N2E2_4360 0.87 -0.6
5,090,913 - -2.2
5,090,913 - -1.4
5,090,954 - +0.2

Or see this region's nucleotide sequence