Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_254 and Pf6N2E2_255 overlap by 4 nucleotides Pf6N2E2_255 and Pf6N2E2_256 overlap by 8 nucleotides Pf6N2E2_256 and Pf6N2E2_257 are separated by 118 nucleotides
Pf6N2E2_254 - Transcriptional regulator, LysR family, at 318,247 to 319,194
_254
Pf6N2E2_255 - DoxX family protein, at 319,191 to 319,604
_255
Pf6N2E2_256 - putative membrane protein, at 319,597 to 320,235
_256
Pf6N2E2_257 - Exoenzymes regulatory protein AepA precursor, at 320,354 to 322,192
_257
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 318.712 kb on + strand, within Pf6N2E2_254 at 318.712 kb on + strand, within Pf6N2E2_254 at 318.817 kb on + strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.988 kb on + strand, within Pf6N2E2_254 at 319.299 kb on + strand, within Pf6N2E2_255 at 319.299 kb on + strand, within Pf6N2E2_255 at 319.299 kb on + strand, within Pf6N2E2_255 at 319.300 kb on - strand, within Pf6N2E2_255 at 319.300 kb on - strand, within Pf6N2E2_255 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.733 kb on + strand, within Pf6N2E2_256 at 319.734 kb on - strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.143 kb on + strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.333 kb on - strand at 320.393 kb on - strand at 320.790 kb on - strand, within Pf6N2E2_257 at 320.834 kb on + strand, within Pf6N2E2_257 at 320.975 kb on + strand, within Pf6N2E2_257 at 321.077 kb on + strand, within Pf6N2E2_257 at 321.078 kb on - strand, within Pf6N2E2_257 at 321.131 kb on + strand, within Pf6N2E2_257 at 321.132 kb on - strand, within Pf6N2E2_257
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 318,712 + Pf6N2E2_254 0.49 +1.2 318,712 + Pf6N2E2_254 0.49 -0.8 318,817 + Pf6N2E2_254 0.60 +0.5 318,818 - Pf6N2E2_254 0.60 +0.8 318,818 - Pf6N2E2_254 0.60 -0.5 318,818 - Pf6N2E2_254 0.60 -0.3 318,818 - Pf6N2E2_254 0.60 -1.2 318,988 + Pf6N2E2_254 0.78 +0.1 319,299 + Pf6N2E2_255 0.26 -1.3 319,299 + Pf6N2E2_255 0.26 +0.7 319,299 + Pf6N2E2_255 0.26 -0.0 319,300 - Pf6N2E2_255 0.26 +1.1 319,300 - Pf6N2E2_255 0.26 +1.2 319,525 + Pf6N2E2_255 0.81 -0.6 319,525 + Pf6N2E2_255 0.81 +0.1 319,526 - Pf6N2E2_255 0.81 +1.1 319,526 - Pf6N2E2_255 0.81 -0.3 319,537 + Pf6N2E2_255 0.84 -0.6 319,537 + Pf6N2E2_255 0.84 +0.7 319,538 - Pf6N2E2_255 0.84 +2.1 319,538 - Pf6N2E2_255 0.84 -0.7 319,733 + Pf6N2E2_256 0.21 -1.1 319,734 - Pf6N2E2_256 0.21 -1.2 320,092 + Pf6N2E2_256 0.77 +0.6 320,092 + Pf6N2E2_256 0.77 -1.0 320,092 + Pf6N2E2_256 0.77 -1.0 320,092 + Pf6N2E2_256 0.77 -1.1 320,093 - Pf6N2E2_256 0.78 -0.5 320,093 - Pf6N2E2_256 0.78 -0.8 320,093 - Pf6N2E2_256 0.78 +0.3 320,093 - Pf6N2E2_256 0.78 +0.1 320,143 + Pf6N2E2_256 0.85 -2.8 320,144 - Pf6N2E2_256 0.86 +1.8 320,144 - Pf6N2E2_256 0.86 -0.7 320,144 - Pf6N2E2_256 0.86 +0.1 320,144 - Pf6N2E2_256 0.86 +0.4 320,144 - Pf6N2E2_256 0.86 +0.1 320,333 - +1.2 320,393 - -1.1 320,790 - Pf6N2E2_257 0.24 +0.0 320,834 + Pf6N2E2_257 0.26 -0.5 320,975 + Pf6N2E2_257 0.34 +0.4 321,077 + Pf6N2E2_257 0.39 -1.0 321,078 - Pf6N2E2_257 0.39 -0.5 321,131 + Pf6N2E2_257 0.42 +1.9 321,132 - Pf6N2E2_257 0.42 -0.1
Or see this region's nucleotide sequence