Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2247 and Pf6N2E2_2248 are separated by 143 nucleotides Pf6N2E2_2248 and Pf6N2E2_2249 are separated by 10 nucleotides
Pf6N2E2_2247 - Transcriptional regulator, AraC family, at 2,660,811 to 2,661,587
_2247
Pf6N2E2_2248 - Aspartate racemase, at 2,661,731 to 2,663,245
_2248
Pf6N2E2_2249 - Proton/glutamate symport protein @ Sodium/glutamate symport protein, at 2,663,256 to 2,664,518
_2249
Position (kb)
2661
2662
2663
2664 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2660.877 kb on + strand at 2660.878 kb on - strand at 2660.878 kb on - strand at 2661.285 kb on + strand, within Pf6N2E2_2247 at 2661.286 kb on - strand, within Pf6N2E2_2247 at 2661.286 kb on - strand, within Pf6N2E2_2247 at 2661.477 kb on + strand, within Pf6N2E2_2247 at 2661.508 kb on - strand, within Pf6N2E2_2247 at 2661.589 kb on - strand at 2661.589 kb on - strand at 2661.657 kb on - strand at 2662.015 kb on - strand, within Pf6N2E2_2248 at 2662.015 kb on - strand, within Pf6N2E2_2248 at 2662.015 kb on - strand, within Pf6N2E2_2248 at 2662.095 kb on + strand, within Pf6N2E2_2248 at 2662.096 kb on - strand, within Pf6N2E2_2248 at 2662.096 kb on - strand, within Pf6N2E2_2248 at 2662.115 kb on - strand, within Pf6N2E2_2248 at 2662.155 kb on + strand, within Pf6N2E2_2248 at 2662.155 kb on + strand, within Pf6N2E2_2248 at 2662.155 kb on + strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.156 kb on - strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.308 kb on + strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.309 kb on - strand, within Pf6N2E2_2248 at 2662.365 kb on - strand, within Pf6N2E2_2248 at 2662.540 kb on - strand, within Pf6N2E2_2248 at 2662.666 kb on - strand, within Pf6N2E2_2248 at 2662.722 kb on + strand, within Pf6N2E2_2248 at 2662.722 kb on + strand, within Pf6N2E2_2248 at 2662.722 kb on + strand, within Pf6N2E2_2248 at 2662.722 kb on + strand, within Pf6N2E2_2248 at 2662.722 kb on + strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.723 kb on - strand, within Pf6N2E2_2248 at 2662.791 kb on + strand, within Pf6N2E2_2248 at 2662.792 kb on - strand, within Pf6N2E2_2248 at 2662.792 kb on - strand, within Pf6N2E2_2248 at 2662.946 kb on - strand, within Pf6N2E2_2248 at 2662.946 kb on - strand, within Pf6N2E2_2248 at 2663.019 kb on + strand, within Pf6N2E2_2248 at 2663.019 kb on + strand, within Pf6N2E2_2248 at 2663.020 kb on - strand, within Pf6N2E2_2248 at 2663.020 kb on - strand, within Pf6N2E2_2248 at 2663.020 kb on - strand, within Pf6N2E2_2248 at 2663.236 kb on + strand at 2663.369 kb on + strand at 2663.516 kb on + strand, within Pf6N2E2_2249 at 2663.578 kb on + strand, within Pf6N2E2_2249 at 2663.578 kb on + strand, within Pf6N2E2_2249 at 2663.579 kb on - strand, within Pf6N2E2_2249 at 2663.614 kb on + strand, within Pf6N2E2_2249 at 2663.614 kb on + strand, within Pf6N2E2_2249 at 2663.614 kb on + strand, within Pf6N2E2_2249 at 2663.614 kb on + strand, within Pf6N2E2_2249 at 2663.614 kb on + strand, within Pf6N2E2_2249 at 2663.614 kb on + strand, within Pf6N2E2_2249 at 2663.615 kb on - strand, within Pf6N2E2_2249 at 2663.615 kb on - strand, within Pf6N2E2_2249 at 2663.615 kb on - strand, within Pf6N2E2_2249 at 2663.615 kb on - strand, within Pf6N2E2_2249 at 2663.615 kb on - strand, within Pf6N2E2_2249 at 2663.615 kb on - strand, within Pf6N2E2_2249 at 2663.813 kb on - strand, within Pf6N2E2_2249 at 2663.857 kb on + strand, within Pf6N2E2_2249 at 2663.858 kb on - strand, within Pf6N2E2_2249 at 2663.858 kb on - strand, within Pf6N2E2_2249 at 2663.858 kb on - strand, within Pf6N2E2_2249 at 2663.882 kb on - strand, within Pf6N2E2_2249 at 2663.912 kb on + strand, within Pf6N2E2_2249 at 2663.938 kb on - strand, within Pf6N2E2_2249 at 2663.965 kb on + strand, within Pf6N2E2_2249 at 2663.965 kb on + strand, within Pf6N2E2_2249 at 2663.966 kb on - strand, within Pf6N2E2_2249
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,660,877 + -0.2 2,660,878 - -0.2 2,660,878 - -2.4 2,661,285 + Pf6N2E2_2247 0.61 -0.3 2,661,286 - Pf6N2E2_2247 0.61 +1.1 2,661,286 - Pf6N2E2_2247 0.61 +0.4 2,661,477 + Pf6N2E2_2247 0.86 +0.8 2,661,508 - Pf6N2E2_2247 0.90 -0.9 2,661,589 - +0.5 2,661,589 - +0.1 2,661,657 - +1.0 2,662,015 - Pf6N2E2_2248 0.19 -0.5 2,662,015 - Pf6N2E2_2248 0.19 -1.5 2,662,015 - Pf6N2E2_2248 0.19 -1.4 2,662,095 + Pf6N2E2_2248 0.24 -0.2 2,662,096 - Pf6N2E2_2248 0.24 -0.9 2,662,096 - Pf6N2E2_2248 0.24 +0.2 2,662,115 - Pf6N2E2_2248 0.25 -1.0 2,662,155 + Pf6N2E2_2248 0.28 -0.2 2,662,155 + Pf6N2E2_2248 0.28 -0.5 2,662,155 + Pf6N2E2_2248 0.28 -0.2 2,662,156 - Pf6N2E2_2248 0.28 -0.2 2,662,156 - Pf6N2E2_2248 0.28 -0.5 2,662,156 - Pf6N2E2_2248 0.28 -0.8 2,662,156 - Pf6N2E2_2248 0.28 -1.6 2,662,156 - Pf6N2E2_2248 0.28 -1.8 2,662,156 - Pf6N2E2_2248 0.28 -2.3 2,662,156 - Pf6N2E2_2248 0.28 -2.0 2,662,308 + Pf6N2E2_2248 0.38 +0.8 2,662,308 + Pf6N2E2_2248 0.38 +0.1 2,662,308 + Pf6N2E2_2248 0.38 -1.0 2,662,308 + Pf6N2E2_2248 0.38 -1.6 2,662,308 + Pf6N2E2_2248 0.38 +0.0 2,662,308 + Pf6N2E2_2248 0.38 -1.2 2,662,308 + Pf6N2E2_2248 0.38 -0.7 2,662,308 + Pf6N2E2_2248 0.38 -1.2 2,662,308 + Pf6N2E2_2248 0.38 -1.8 2,662,309 - Pf6N2E2_2248 0.38 -0.4 2,662,309 - Pf6N2E2_2248 0.38 -1.4 2,662,309 - Pf6N2E2_2248 0.38 -2.4 2,662,309 - Pf6N2E2_2248 0.38 -1.7 2,662,309 - Pf6N2E2_2248 0.38 -3.2 2,662,309 - Pf6N2E2_2248 0.38 -2.1 2,662,309 - Pf6N2E2_2248 0.38 -2.5 2,662,365 - Pf6N2E2_2248 0.42 -1.6 2,662,540 - Pf6N2E2_2248 0.53 -0.7 2,662,666 - Pf6N2E2_2248 0.62 -1.5 2,662,722 + Pf6N2E2_2248 0.65 +0.8 2,662,722 + Pf6N2E2_2248 0.65 -0.8 2,662,722 + Pf6N2E2_2248 0.65 -1.1 2,662,722 + Pf6N2E2_2248 0.65 -1.7 2,662,722 + Pf6N2E2_2248 0.65 -0.4 2,662,723 - Pf6N2E2_2248 0.65 -1.2 2,662,723 - Pf6N2E2_2248 0.65 -2.2 2,662,723 - Pf6N2E2_2248 0.65 -1.2 2,662,723 - Pf6N2E2_2248 0.65 -0.4 2,662,723 - Pf6N2E2_2248 0.65 -1.1 2,662,723 - Pf6N2E2_2248 0.65 -1.6 2,662,723 - Pf6N2E2_2248 0.65 +0.3 2,662,723 - Pf6N2E2_2248 0.65 -1.7 2,662,791 + Pf6N2E2_2248 0.70 -2.0 2,662,792 - Pf6N2E2_2248 0.70 -0.7 2,662,792 - Pf6N2E2_2248 0.70 -1.4 2,662,946 - Pf6N2E2_2248 0.80 -0.7 2,662,946 - Pf6N2E2_2248 0.80 -2.3 2,663,019 + Pf6N2E2_2248 0.85 -2.9 2,663,019 + Pf6N2E2_2248 0.85 +0.1 2,663,020 - Pf6N2E2_2248 0.85 -2.0 2,663,020 - Pf6N2E2_2248 0.85 -0.8 2,663,020 - Pf6N2E2_2248 0.85 +0.6 2,663,236 + -0.7 2,663,369 + +0.6 2,663,516 + Pf6N2E2_2249 0.21 +1.3 2,663,578 + Pf6N2E2_2249 0.25 -1.4 2,663,578 + Pf6N2E2_2249 0.25 +0.3 2,663,579 - Pf6N2E2_2249 0.26 +0.1 2,663,614 + Pf6N2E2_2249 0.28 -0.4 2,663,614 + Pf6N2E2_2249 0.28 -1.4 2,663,614 + Pf6N2E2_2249 0.28 -0.8 2,663,614 + Pf6N2E2_2249 0.28 +0.4 2,663,614 + Pf6N2E2_2249 0.28 +0.5 2,663,614 + Pf6N2E2_2249 0.28 +0.1 2,663,615 - Pf6N2E2_2249 0.28 +1.0 2,663,615 - Pf6N2E2_2249 0.28 +0.2 2,663,615 - Pf6N2E2_2249 0.28 +0.6 2,663,615 - Pf6N2E2_2249 0.28 -2.6 2,663,615 - Pf6N2E2_2249 0.28 +0.3 2,663,615 - Pf6N2E2_2249 0.28 +0.4 2,663,813 - Pf6N2E2_2249 0.44 -0.7 2,663,857 + Pf6N2E2_2249 0.48 +1.4 2,663,858 - Pf6N2E2_2249 0.48 -1.2 2,663,858 - Pf6N2E2_2249 0.48 +0.8 2,663,858 - Pf6N2E2_2249 0.48 +1.3 2,663,882 - Pf6N2E2_2249 0.50 -1.2 2,663,912 + Pf6N2E2_2249 0.52 +0.1 2,663,938 - Pf6N2E2_2249 0.54 -0.4 2,663,965 + Pf6N2E2_2249 0.56 +0.5 2,663,965 + Pf6N2E2_2249 0.56 -0.6 2,663,966 - Pf6N2E2_2249 0.56 +0.1
Or see this region's nucleotide sequence