Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1430 and Pf6N2E2_1431 are separated by 72 nucleotides Pf6N2E2_1431 and Pf6N2E2_1432 are separated by 11 nucleotides Pf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotides
Pf6N2E2_1430 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 1,679,300 to 1,680,625
_1430
Pf6N2E2_1431 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,680,698 to 1,681,765
_1431
Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658
_1432
Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508
_1433
Position (kb)
1680
1681
1682 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1679.720 kb on + strand, within Pf6N2E2_1430 at 1679.721 kb on - strand, within Pf6N2E2_1430 at 1679.957 kb on - strand, within Pf6N2E2_1430 at 1680.024 kb on + strand, within Pf6N2E2_1430 at 1680.084 kb on + strand, within Pf6N2E2_1430 at 1680.085 kb on - strand, within Pf6N2E2_1430 at 1680.144 kb on + strand, within Pf6N2E2_1430 at 1680.145 kb on - strand, within Pf6N2E2_1430 at 1680.148 kb on + strand, within Pf6N2E2_1430 at 1680.148 kb on + strand, within Pf6N2E2_1430 at 1680.149 kb on - strand, within Pf6N2E2_1430 at 1680.149 kb on - strand, within Pf6N2E2_1430 at 1680.149 kb on - strand, within Pf6N2E2_1430 at 1680.299 kb on - strand, within Pf6N2E2_1430 at 1680.363 kb on + strand, within Pf6N2E2_1430 at 1680.364 kb on - strand, within Pf6N2E2_1430 at 1680.391 kb on - strand, within Pf6N2E2_1430 at 1680.391 kb on - strand, within Pf6N2E2_1430 at 1680.391 kb on - strand, within Pf6N2E2_1430 at 1680.454 kb on - strand, within Pf6N2E2_1430 at 1680.454 kb on - strand, within Pf6N2E2_1430 at 1680.462 kb on + strand, within Pf6N2E2_1430 at 1680.462 kb on + strand, within Pf6N2E2_1430 at 1680.463 kb on - strand, within Pf6N2E2_1430 at 1680.537 kb on + strand at 1680.537 kb on + strand at 1680.538 kb on - strand at 1680.538 kb on - strand at 1680.538 kb on - strand at 1680.538 kb on - strand at 1680.641 kb on - strand at 1680.641 kb on - strand at 1680.655 kb on - strand at 1681.371 kb on + strand, within Pf6N2E2_1431 at 1681.372 kb on - strand, within Pf6N2E2_1431 at 1681.492 kb on - strand, within Pf6N2E2_1431 at 1681.779 kb on - strand at 1681.895 kb on + strand, within Pf6N2E2_1432 at 1681.896 kb on - strand, within Pf6N2E2_1432 at 1681.963 kb on - strand, within Pf6N2E2_1432 at 1682.351 kb on + strand, within Pf6N2E2_1432 at 1682.351 kb on + strand, within Pf6N2E2_1432 at 1682.352 kb on - strand, within Pf6N2E2_1432 at 1682.573 kb on + strand at 1682.606 kb on + strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.638 kb on + strand at 1682.638 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,679,720 + Pf6N2E2_1430 0.32 +0.6 1,679,721 - Pf6N2E2_1430 0.32 +1.0 1,679,957 - Pf6N2E2_1430 0.50 -1.6 1,680,024 + Pf6N2E2_1430 0.55 -1.2 1,680,084 + Pf6N2E2_1430 0.59 +0.9 1,680,085 - Pf6N2E2_1430 0.59 -0.5 1,680,144 + Pf6N2E2_1430 0.64 +2.6 1,680,145 - Pf6N2E2_1430 0.64 -0.6 1,680,148 + Pf6N2E2_1430 0.64 +0.8 1,680,148 + Pf6N2E2_1430 0.64 -0.3 1,680,149 - Pf6N2E2_1430 0.64 -0.1 1,680,149 - Pf6N2E2_1430 0.64 -0.3 1,680,149 - Pf6N2E2_1430 0.64 +1.2 1,680,299 - Pf6N2E2_1430 0.75 -0.4 1,680,363 + Pf6N2E2_1430 0.80 +0.4 1,680,364 - Pf6N2E2_1430 0.80 +0.3 1,680,391 - Pf6N2E2_1430 0.82 +0.0 1,680,391 - Pf6N2E2_1430 0.82 -2.1 1,680,391 - Pf6N2E2_1430 0.82 +0.2 1,680,454 - Pf6N2E2_1430 0.87 +0.6 1,680,454 - Pf6N2E2_1430 0.87 +0.6 1,680,462 + Pf6N2E2_1430 0.88 -1.2 1,680,462 + Pf6N2E2_1430 0.88 +1.0 1,680,463 - Pf6N2E2_1430 0.88 -0.3 1,680,537 + -1.9 1,680,537 + +0.6 1,680,538 - -1.4 1,680,538 - +0.8 1,680,538 - -0.5 1,680,538 - +1.2 1,680,641 - +0.2 1,680,641 - +0.6 1,680,655 - +0.2 1,681,371 + Pf6N2E2_1431 0.63 -1.1 1,681,372 - Pf6N2E2_1431 0.63 -0.2 1,681,492 - Pf6N2E2_1431 0.74 -1.6 1,681,779 - +0.6 1,681,895 + Pf6N2E2_1432 0.13 -1.4 1,681,896 - Pf6N2E2_1432 0.13 +0.5 1,681,963 - Pf6N2E2_1432 0.21 +0.4 1,682,351 + Pf6N2E2_1432 0.65 +0.2 1,682,351 + Pf6N2E2_1432 0.65 +1.5 1,682,352 - Pf6N2E2_1432 0.65 -0.0 1,682,573 + +0.2 1,682,606 + +0.7 1,682,607 - +0.6 1,682,607 - -0.3 1,682,607 - -1.0 1,682,607 - +1.3 1,682,607 - -0.1 1,682,607 - +0.6 1,682,638 + -0.7 1,682,638 + +0.5
Or see this region's nucleotide sequence