Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1127 and Pf6N2E2_1128 overlap by 10 nucleotides Pf6N2E2_1128 and Pf6N2E2_1129 overlap by 4 nucleotides
Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632
_1127
Pf6N2E2_1128 - Two-component response regulator, at 1,324,623 to 1,325,291
_1128
Pf6N2E2_1129 - Sensor histidine kinase, at 1,325,288 to 1,326,472
_1129
Position (kb)
1324
1325
1326 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1323.671 kb on + strand, within Pf6N2E2_1127 at 1323.672 kb on - strand, within Pf6N2E2_1127 at 1323.672 kb on - strand, within Pf6N2E2_1127 at 1323.714 kb on - strand, within Pf6N2E2_1127 at 1323.756 kb on - strand, within Pf6N2E2_1127 at 1323.899 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.397 kb on + strand, within Pf6N2E2_1127 at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.406 kb on - strand at 1324.435 kb on - strand at 1324.516 kb on - strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.726 kb on - strand, within Pf6N2E2_1128 at 1324.982 kb on - strand, within Pf6N2E2_1128 at 1325.231 kb on + strand at 1325.688 kb on + strand, within Pf6N2E2_1129 at 1325.688 kb on + strand, within Pf6N2E2_1129 at 1325.708 kb on + strand, within Pf6N2E2_1129 at 1325.890 kb on - strand, within Pf6N2E2_1129 at 1326.101 kb on + strand, within Pf6N2E2_1129 at 1326.101 kb on + strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,323,671 + Pf6N2E2_1127 0.59 -1.6 1,323,672 - Pf6N2E2_1127 0.59 -0.3 1,323,672 - Pf6N2E2_1127 0.59 -2.9 1,323,714 - Pf6N2E2_1127 0.60 -0.4 1,323,756 - Pf6N2E2_1127 0.62 -0.5 1,323,899 - Pf6N2E2_1127 0.68 -3.9 1,324,362 - Pf6N2E2_1127 0.88 -1.6 1,324,362 - Pf6N2E2_1127 0.88 +0.3 1,324,362 - Pf6N2E2_1127 0.88 +0.1 1,324,397 + Pf6N2E2_1127 0.90 +0.7 1,324,405 + +0.2 1,324,405 + +0.1 1,324,405 + +1.0 1,324,406 - +2.3 1,324,435 - -0.1 1,324,516 - +0.5 1,324,517 + +0.7 1,324,517 + +0.1 1,324,517 + +0.5 1,324,518 - -1.4 1,324,518 - -0.6 1,324,518 - +0.1 1,324,518 - +0.1 1,324,518 - +0.3 1,324,726 - Pf6N2E2_1128 0.15 -1.2 1,324,982 - Pf6N2E2_1128 0.54 -0.4 1,325,231 + -1.0 1,325,688 + Pf6N2E2_1129 0.34 -0.4 1,325,688 + Pf6N2E2_1129 0.34 -0.4 1,325,708 + Pf6N2E2_1129 0.35 -0.2 1,325,890 - Pf6N2E2_1129 0.51 +0.0 1,326,101 + Pf6N2E2_1129 0.69 +0.6 1,326,101 + Pf6N2E2_1129 0.69 -1.3 1,326,102 - Pf6N2E2_1129 0.69 +2.2 1,326,102 - Pf6N2E2_1129 0.69 -0.0 1,326,102 - Pf6N2E2_1129 0.69 -0.1 1,326,102 - Pf6N2E2_1129 0.69 -0.6
Or see this region's nucleotide sequence