Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5844 and Pf6N2E2_5845 are separated by 134 nucleotides Pf6N2E2_5845 and Pf6N2E2_5846 are separated by 25 nucleotides Pf6N2E2_5846 and Pf6N2E2_5847 are separated by 422 nucleotides
Pf6N2E2_5844 - Membrane protein, putative, at 6,659,730 to 6,660,644
_5844
Pf6N2E2_5845 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 6,660,779 to 6,661,534
_5845
Pf6N2E2_5846 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 6,661,560 to 6,662,858
_5846
Pf6N2E2_5847 - Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline- binding/permease protein, at 6,663,281 to 6,664,135
_5847
Position (kb)
6661
6662
6663 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6660.642 kb on + strand at 6660.642 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.710 kb on - strand at 6660.780 kb on + strand at 6660.780 kb on + strand at 6660.814 kb on - strand at 6660.819 kb on + strand at 6660.819 kb on + strand at 6660.820 kb on - strand at 6660.820 kb on - strand at 6660.834 kb on + strand at 6660.949 kb on - strand, within Pf6N2E2_5845 at 6660.949 kb on - strand, within Pf6N2E2_5845 at 6660.973 kb on - strand, within Pf6N2E2_5845 at 6661.029 kb on - strand, within Pf6N2E2_5845 at 6661.029 kb on - strand, within Pf6N2E2_5845 at 6661.035 kb on + strand, within Pf6N2E2_5845 at 6661.036 kb on - strand, within Pf6N2E2_5845 at 6661.056 kb on + strand, within Pf6N2E2_5845 at 6661.056 kb on + strand, within Pf6N2E2_5845 at 6661.344 kb on + strand, within Pf6N2E2_5845 at 6661.377 kb on + strand, within Pf6N2E2_5845 at 6661.377 kb on + strand, within Pf6N2E2_5845 at 6661.377 kb on + strand, within Pf6N2E2_5845 at 6661.378 kb on - strand, within Pf6N2E2_5845 at 6661.378 kb on - strand, within Pf6N2E2_5845 at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.747 kb on + strand, within Pf6N2E2_5846 at 6661.748 kb on - strand, within Pf6N2E2_5846 at 6661.762 kb on + strand, within Pf6N2E2_5846 at 6661.846 kb on + strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6662.057 kb on - strand, within Pf6N2E2_5846 at 6662.083 kb on + strand, within Pf6N2E2_5846 at 6662.101 kb on + strand, within Pf6N2E2_5846 at 6662.102 kb on - strand, within Pf6N2E2_5846 at 6662.122 kb on - strand, within Pf6N2E2_5846 at 6662.131 kb on + strand, within Pf6N2E2_5846 at 6662.203 kb on + strand, within Pf6N2E2_5846 at 6662.203 kb on + strand, within Pf6N2E2_5846 at 6662.203 kb on + strand, within Pf6N2E2_5846 at 6662.204 kb on - strand, within Pf6N2E2_5846 at 6662.204 kb on - strand, within Pf6N2E2_5846 at 6662.204 kb on - strand, within Pf6N2E2_5846 at 6662.320 kb on + strand, within Pf6N2E2_5846 at 6662.321 kb on - strand, within Pf6N2E2_5846 at 6662.323 kb on + strand, within Pf6N2E2_5846 at 6662.324 kb on - strand, within Pf6N2E2_5846 at 6662.324 kb on - strand, within Pf6N2E2_5846 at 6662.324 kb on - strand, within Pf6N2E2_5846 at 6662.377 kb on + strand, within Pf6N2E2_5846 at 6662.377 kb on + strand, within Pf6N2E2_5846 at 6662.378 kb on - strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.509 kb on + strand, within Pf6N2E2_5846 at 6662.510 kb on - strand, within Pf6N2E2_5846 at 6662.510 kb on - strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.523 kb on - strand, within Pf6N2E2_5846 at 6662.770 kb on + strand at 6662.770 kb on + strand at 6662.770 kb on + strand at 6662.771 kb on - strand at 6662.771 kb on - strand at 6662.771 kb on - strand at 6662.817 kb on - strand at 6662.871 kb on + strand at 6662.872 kb on - strand at 6662.872 kb on - strand at 6662.902 kb on + strand at 6662.903 kb on - strand at 6662.965 kb on + strand at 6662.968 kb on + strand at 6663.004 kb on - strand at 6663.008 kb on - strand at 6663.008 kb on - strand at 6663.035 kb on - strand at 6663.056 kb on + strand at 6663.056 kb on + strand at 6663.057 kb on - strand at 6663.192 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.223 kb on - strand at 6663.223 kb on - strand at 6663.223 kb on - strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.227 kb on - strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.229 kb on - strand at 6663.229 kb on - strand at 6663.229 kb on - strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.277 kb on - strand at 6663.449 kb on + strand, within Pf6N2E2_5847 at 6663.557 kb on + strand, within Pf6N2E2_5847 at 6663.591 kb on - strand, within Pf6N2E2_5847 at 6663.638 kb on + strand, within Pf6N2E2_5847 at 6663.756 kb on - strand, within Pf6N2E2_5847 at 6663.756 kb on - strand, within Pf6N2E2_5847
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 6,660,642 + +0.1 6,660,642 + -1.2 6,660,709 + -0.2 6,660,709 + -0.0 6,660,709 + -0.2 6,660,709 + +0.6 6,660,709 + +0.5 6,660,709 + -2.3 6,660,709 + -0.3 6,660,709 + +0.1 6,660,710 - -0.5 6,660,780 + -1.0 6,660,780 + -0.4 6,660,814 - -0.4 6,660,819 + -0.2 6,660,819 + -0.5 6,660,820 - -1.1 6,660,820 - -3.0 6,660,834 + -1.4 6,660,949 - Pf6N2E2_5845 0.22 -2.1 6,660,949 - Pf6N2E2_5845 0.22 -1.8 6,660,973 - Pf6N2E2_5845 0.26 -1.3 6,661,029 - Pf6N2E2_5845 0.33 -1.8 6,661,029 - Pf6N2E2_5845 0.33 -2.6 6,661,035 + Pf6N2E2_5845 0.34 -0.8 6,661,036 - Pf6N2E2_5845 0.34 -1.3 6,661,056 + Pf6N2E2_5845 0.37 -4.0 6,661,056 + Pf6N2E2_5845 0.37 -2.1 6,661,344 + Pf6N2E2_5845 0.75 -3.6 6,661,377 + Pf6N2E2_5845 0.79 -2.7 6,661,377 + Pf6N2E2_5845 0.79 -2.3 6,661,377 + Pf6N2E2_5845 0.79 -1.8 6,661,378 - Pf6N2E2_5845 0.79 -0.4 6,661,378 - Pf6N2E2_5845 0.79 -1.6 6,661,557 + -1.8 6,661,557 + -2.8 6,661,557 + -1.2 6,661,557 + -3.1 6,661,557 + +0.2 6,661,558 - -0.0 6,661,558 - -2.6 6,661,558 - -4.3 6,661,558 - -1.6 6,661,558 - -1.1 6,661,558 - -0.7 6,661,558 - -1.7 6,661,607 + -1.4 6,661,607 + -1.4 6,661,607 + -1.7 6,661,607 + -1.7 6,661,607 + -1.9 6,661,608 - -2.0 6,661,608 - -0.5 6,661,608 - -1.6 6,661,608 - -2.7 6,661,608 - -1.7 6,661,747 + Pf6N2E2_5846 0.14 -0.3 6,661,748 - Pf6N2E2_5846 0.14 -1.6 6,661,762 + Pf6N2E2_5846 0.16 -0.9 6,661,846 + Pf6N2E2_5846 0.22 -3.8 6,661,847 - Pf6N2E2_5846 0.22 +0.2 6,661,847 - Pf6N2E2_5846 0.22 -1.7 6,661,847 - Pf6N2E2_5846 0.22 -0.6 6,661,847 - Pf6N2E2_5846 0.22 -2.1 6,662,057 - Pf6N2E2_5846 0.38 -2.5 6,662,083 + Pf6N2E2_5846 0.40 -0.4 6,662,101 + Pf6N2E2_5846 0.42 -1.5 6,662,102 - Pf6N2E2_5846 0.42 -1.0 6,662,122 - Pf6N2E2_5846 0.43 -2.7 6,662,131 + Pf6N2E2_5846 0.44 -1.8 6,662,203 + Pf6N2E2_5846 0.49 -1.1 6,662,203 + Pf6N2E2_5846 0.49 -1.4 6,662,203 + Pf6N2E2_5846 0.49 -1.0 6,662,204 - Pf6N2E2_5846 0.50 +1.6 6,662,204 - Pf6N2E2_5846 0.50 -2.0 6,662,204 - Pf6N2E2_5846 0.50 -1.2 6,662,320 + Pf6N2E2_5846 0.59 -2.1 6,662,321 - Pf6N2E2_5846 0.59 -1.8 6,662,323 + Pf6N2E2_5846 0.59 -1.7 6,662,324 - Pf6N2E2_5846 0.59 -0.1 6,662,324 - Pf6N2E2_5846 0.59 -1.6 6,662,324 - Pf6N2E2_5846 0.59 -3.0 6,662,377 + Pf6N2E2_5846 0.63 -1.0 6,662,377 + Pf6N2E2_5846 0.63 -1.6 6,662,378 - Pf6N2E2_5846 0.63 -1.3 6,662,491 + Pf6N2E2_5846 0.72 -0.9 6,662,491 + Pf6N2E2_5846 0.72 -1.6 6,662,491 + Pf6N2E2_5846 0.72 -2.8 6,662,491 + Pf6N2E2_5846 0.72 -0.9 6,662,492 - Pf6N2E2_5846 0.72 -1.6 6,662,492 - Pf6N2E2_5846 0.72 -1.4 6,662,492 - Pf6N2E2_5846 0.72 -0.9 6,662,492 - Pf6N2E2_5846 0.72 +0.1 6,662,492 - Pf6N2E2_5846 0.72 -2.5 6,662,492 - Pf6N2E2_5846 0.72 -3.6 6,662,495 - Pf6N2E2_5846 0.72 -1.6 6,662,495 - Pf6N2E2_5846 0.72 -4.3 6,662,495 - Pf6N2E2_5846 0.72 -2.3 6,662,495 - Pf6N2E2_5846 0.72 -1.2 6,662,509 + Pf6N2E2_5846 0.73 -1.5 6,662,510 - Pf6N2E2_5846 0.73 -2.2 6,662,510 - Pf6N2E2_5846 0.73 -1.1 6,662,522 + Pf6N2E2_5846 0.74 -2.0 6,662,522 + Pf6N2E2_5846 0.74 -2.2 6,662,522 + Pf6N2E2_5846 0.74 -1.5 6,662,522 + Pf6N2E2_5846 0.74 -0.4 6,662,522 + Pf6N2E2_5846 0.74 -1.3 6,662,522 + Pf6N2E2_5846 0.74 -2.0 6,662,522 + Pf6N2E2_5846 0.74 +0.3 6,662,522 + Pf6N2E2_5846 0.74 -1.5 6,662,523 - Pf6N2E2_5846 0.74 -1.8 6,662,770 + +0.6 6,662,770 + -2.0 6,662,770 + -3.1 6,662,771 - +0.6 6,662,771 - -1.2 6,662,771 - -1.4 6,662,817 - -1.4 6,662,871 + -1.6 6,662,872 - +1.3 6,662,872 - +1.3 6,662,902 + -1.2 6,662,903 - +0.4 6,662,965 + +0.6 6,662,968 + -1.0 6,663,004 - +0.4 6,663,008 - +0.6 6,663,008 - +2.1 6,663,035 - +0.6 6,663,056 + +0.7 6,663,056 + +1.3 6,663,057 - +1.0 6,663,192 + -0.4 6,663,222 + +0.0 6,663,222 + +0.4 6,663,222 + -0.6 6,663,222 + -0.4 6,663,222 + +0.4 6,663,222 + -0.4 6,663,222 + -0.5 6,663,222 + -0.2 6,663,223 - +0.6 6,663,223 - +0.6 6,663,223 - +0.8 6,663,226 + +0.9 6,663,226 + +0.8 6,663,226 + +0.2 6,663,226 + +0.3 6,663,226 + -0.3 6,663,226 + -1.0 6,663,227 - +0.6 6,663,228 + -3.8 6,663,228 + +0.6 6,663,228 + -1.1 6,663,228 + +0.7 6,663,228 + +0.8 6,663,228 + +0.9 6,663,229 - -0.2 6,663,229 - +0.8 6,663,229 - -0.1 6,663,276 + +0.7 6,663,276 + -0.1 6,663,276 + +1.0 6,663,276 + -0.3 6,663,276 + +1.1 6,663,277 - +0.8 6,663,449 + Pf6N2E2_5847 0.20 -0.1 6,663,557 + Pf6N2E2_5847 0.32 +0.9 6,663,591 - Pf6N2E2_5847 0.36 +1.0 6,663,638 + Pf6N2E2_5847 0.42 +0.0 6,663,756 - Pf6N2E2_5847 0.56 +0.7 6,663,756 - Pf6N2E2_5847 0.56 +0.6
Or see this region's nucleotide sequence