Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5739 and Pf6N2E2_5740 overlap by 19 nucleotides Pf6N2E2_5740 and Pf6N2E2_5741 overlap by 8 nucleotides Pf6N2E2_5741 and Pf6N2E2_5742 overlap by 4 nucleotides
Pf6N2E2_5739 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 6,556,184 to 6,556,834
_5739
Pf6N2E2_5740 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 6,556,816 to 6,557,739
_5740
Pf6N2E2_5741 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 6,557,732 to 6,558,724
_5741
Pf6N2E2_5742 - Cobyric acid synthase (EC 6.3.5.10), at 6,558,721 to 6,560,172
_5742
Position (kb)
6557
6558
6559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6556.743 kb on - strand, within Pf6N2E2_5739 at 6556.805 kb on + strand at 6556.805 kb on + strand at 6556.805 kb on + strand at 6556.805 kb on + strand at 6557.084 kb on - strand, within Pf6N2E2_5740 at 6557.326 kb on + strand, within Pf6N2E2_5740 at 6557.327 kb on - strand, within Pf6N2E2_5740 at 6557.341 kb on - strand, within Pf6N2E2_5740 at 6557.341 kb on - strand, within Pf6N2E2_5740 at 6557.425 kb on + strand, within Pf6N2E2_5740 at 6557.425 kb on + strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.455 kb on + strand, within Pf6N2E2_5740 at 6557.690 kb on + strand at 6557.691 kb on - strand at 6557.691 kb on - strand at 6557.691 kb on - strand at 6557.731 kb on + strand at 6557.731 kb on + strand at 6557.731 kb on + strand at 6557.913 kb on - strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.932 kb on - strand, within Pf6N2E2_5741 at 6557.932 kb on - strand, within Pf6N2E2_5741 at 6557.932 kb on - strand, within Pf6N2E2_5741 at 6558.053 kb on + strand, within Pf6N2E2_5741 at 6558.053 kb on + strand, within Pf6N2E2_5741 at 6558.342 kb on + strand, within Pf6N2E2_5741 at 6558.343 kb on - strand, within Pf6N2E2_5741 at 6558.343 kb on - strand, within Pf6N2E2_5741 at 6558.343 kb on - strand, within Pf6N2E2_5741 at 6558.626 kb on - strand at 6558.697 kb on - strand at 6558.697 kb on - strand at 6558.697 kb on - strand at 6558.704 kb on + strand at 6558.704 kb on + strand at 6558.705 kb on - strand at 6558.785 kb on + strand at 6559.027 kb on + strand, within Pf6N2E2_5742 at 6559.028 kb on - strand, within Pf6N2E2_5742 at 6559.028 kb on - strand, within Pf6N2E2_5742 at 6559.036 kb on + strand, within Pf6N2E2_5742 at 6559.230 kb on - strand, within Pf6N2E2_5742 at 6559.393 kb on + strand, within Pf6N2E2_5742 at 6559.633 kb on + strand, within Pf6N2E2_5742 at 6559.633 kb on + strand, within Pf6N2E2_5742 at 6559.633 kb on + strand, within Pf6N2E2_5742
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 6,556,743 - Pf6N2E2_5739 0.86 -0.6 6,556,805 + +0.7 6,556,805 + +1.2 6,556,805 + +0.3 6,556,805 + +0.8 6,557,084 - Pf6N2E2_5740 0.29 -1.4 6,557,326 + Pf6N2E2_5740 0.55 -1.4 6,557,327 - Pf6N2E2_5740 0.55 -3.3 6,557,341 - Pf6N2E2_5740 0.57 -0.7 6,557,341 - Pf6N2E2_5740 0.57 -0.0 6,557,425 + Pf6N2E2_5740 0.66 -0.3 6,557,425 + Pf6N2E2_5740 0.66 -2.5 6,557,426 - Pf6N2E2_5740 0.66 -0.9 6,557,426 - Pf6N2E2_5740 0.66 +0.5 6,557,426 - Pf6N2E2_5740 0.66 -0.3 6,557,455 + Pf6N2E2_5740 0.69 -1.2 6,557,690 + -1.4 6,557,691 - +0.7 6,557,691 - -1.3 6,557,691 - -1.2 6,557,731 + +0.2 6,557,731 + -0.1 6,557,731 + +0.9 6,557,913 - Pf6N2E2_5741 0.18 -1.3 6,557,915 + Pf6N2E2_5741 0.18 -0.9 6,557,915 + Pf6N2E2_5741 0.18 -1.0 6,557,915 + Pf6N2E2_5741 0.18 -2.3 6,557,915 + Pf6N2E2_5741 0.18 +0.4 6,557,931 + Pf6N2E2_5741 0.20 -0.9 6,557,931 + Pf6N2E2_5741 0.20 -0.2 6,557,931 + Pf6N2E2_5741 0.20 +0.3 6,557,931 + Pf6N2E2_5741 0.20 -1.0 6,557,932 - Pf6N2E2_5741 0.20 -2.8 6,557,932 - Pf6N2E2_5741 0.20 -1.8 6,557,932 - Pf6N2E2_5741 0.20 -2.3 6,558,053 + Pf6N2E2_5741 0.32 -1.4 6,558,053 + Pf6N2E2_5741 0.32 -0.5 6,558,342 + Pf6N2E2_5741 0.61 +0.5 6,558,343 - Pf6N2E2_5741 0.62 -1.7 6,558,343 - Pf6N2E2_5741 0.62 -2.2 6,558,343 - Pf6N2E2_5741 0.62 -2.6 6,558,626 - -0.7 6,558,697 - -1.7 6,558,697 - -2.8 6,558,697 - -0.6 6,558,704 + +0.6 6,558,704 + -0.8 6,558,705 - -1.5 6,558,785 + +2.3 6,559,027 + Pf6N2E2_5742 0.21 -2.6 6,559,028 - Pf6N2E2_5742 0.21 -0.4 6,559,028 - Pf6N2E2_5742 0.21 -1.6 6,559,036 + Pf6N2E2_5742 0.22 -3.3 6,559,230 - Pf6N2E2_5742 0.35 -3.4 6,559,393 + Pf6N2E2_5742 0.46 -0.5 6,559,633 + Pf6N2E2_5742 0.63 -0.9 6,559,633 + Pf6N2E2_5742 0.63 -1.7 6,559,633 + Pf6N2E2_5742 0.63 -1.5
Or see this region's nucleotide sequence