Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4027 and Pf6N2E2_4028 are separated by 67 nucleotides Pf6N2E2_4028 and Pf6N2E2_4029 overlap by 4 nucleotides Pf6N2E2_4029 and Pf6N2E2_4030 are separated by 1 nucleotides
Pf6N2E2_4027 - Ferredoxin reductase, at 4,698,874 to 4,700,406
_4027
Pf6N2E2_4028 - Biofilm PGA synthesis auxiliary protein PgaD, at 4,700,474 to 4,700,980
_4028
Pf6N2E2_4029 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 4,700,977 to 4,702,332
_4029
Pf6N2E2_4030 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 4,702,334 to 4,704,331
_4030
Position (kb)
4700
4701
4702
4703 Strain fitness (log2 ratio)
-2
-1
0
1 at 4700.021 kb on + strand, within Pf6N2E2_4027 at 4700.147 kb on - strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.174 kb on - strand, within Pf6N2E2_4027 at 4700.174 kb on - strand, within Pf6N2E2_4027 at 4700.191 kb on + strand, within Pf6N2E2_4027 at 4700.192 kb on - strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.198 kb on - strand, within Pf6N2E2_4027 at 4700.198 kb on - strand, within Pf6N2E2_4027 at 4700.198 kb on - strand, within Pf6N2E2_4027 at 4700.262 kb on + strand at 4700.262 kb on + strand at 4700.262 kb on + strand at 4700.262 kb on + strand at 4700.263 kb on - strand at 4700.404 kb on + strand at 4700.404 kb on + strand at 4700.404 kb on + strand at 4700.404 kb on + strand at 4700.405 kb on - strand at 4700.405 kb on - strand at 4700.480 kb on + strand at 4700.480 kb on + strand at 4700.481 kb on - strand at 4700.481 kb on - strand at 4700.481 kb on - strand at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.737 kb on + strand, within Pf6N2E2_4028 at 4700.799 kb on + strand, within Pf6N2E2_4028 at 4700.799 kb on + strand, within Pf6N2E2_4028 at 4700.800 kb on - strand, within Pf6N2E2_4028 at 4700.800 kb on - strand, within Pf6N2E2_4028 at 4700.800 kb on - strand, within Pf6N2E2_4028 at 4700.895 kb on + strand, within Pf6N2E2_4028 at 4700.895 kb on + strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.918 kb on + strand, within Pf6N2E2_4028 at 4700.919 kb on - strand, within Pf6N2E2_4028 at 4700.919 kb on - strand, within Pf6N2E2_4028 at 4700.919 kb on - strand, within Pf6N2E2_4028 at 4700.972 kb on - strand at 4701.068 kb on - strand at 4701.068 kb on - strand at 4701.068 kb on - strand at 4701.356 kb on + strand, within Pf6N2E2_4029 at 4701.380 kb on + strand, within Pf6N2E2_4029 at 4701.518 kb on + strand, within Pf6N2E2_4029 at 4701.519 kb on - strand, within Pf6N2E2_4029 at 4701.519 kb on - strand, within Pf6N2E2_4029 at 4701.727 kb on + strand, within Pf6N2E2_4029 at 4701.810 kb on - strand, within Pf6N2E2_4029 at 4701.860 kb on + strand, within Pf6N2E2_4029 at 4701.860 kb on + strand, within Pf6N2E2_4029 at 4701.861 kb on - strand, within Pf6N2E2_4029 at 4702.181 kb on + strand, within Pf6N2E2_4029 at 4702.181 kb on + strand, within Pf6N2E2_4029 at 4702.182 kb on - strand, within Pf6N2E2_4029 at 4702.182 kb on - strand, within Pf6N2E2_4029 at 4702.410 kb on + strand at 4702.410 kb on + strand at 4702.413 kb on + strand at 4702.435 kb on + strand at 4702.436 kb on - strand at 4702.436 kb on - strand at 4702.891 kb on - strand, within Pf6N2E2_4030 at 4702.891 kb on - strand, within Pf6N2E2_4030 at 4702.951 kb on - strand, within Pf6N2E2_4030 at 4702.992 kb on + strand, within Pf6N2E2_4030 at 4702.992 kb on + strand, within Pf6N2E2_4030 at 4702.993 kb on - strand, within Pf6N2E2_4030 at 4703.046 kb on + strand, within Pf6N2E2_4030 at 4703.046 kb on + strand, within Pf6N2E2_4030 at 4703.046 kb on + strand, within Pf6N2E2_4030 at 4703.047 kb on - strand, within Pf6N2E2_4030 at 4703.047 kb on - strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.149 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.293 kb on + strand, within Pf6N2E2_4030 at 4703.293 kb on + strand, within Pf6N2E2_4030 at 4703.294 kb on - strand, within Pf6N2E2_4030 at 4703.294 kb on - strand, within Pf6N2E2_4030 at 4703.314 kb on + strand, within Pf6N2E2_4030 at 4703.314 kb on + strand, within Pf6N2E2_4030
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 4,700,021 + Pf6N2E2_4027 0.75 -1.0 4,700,147 - Pf6N2E2_4027 0.83 -0.1 4,700,173 + Pf6N2E2_4027 0.85 +0.5 4,700,173 + Pf6N2E2_4027 0.85 -1.7 4,700,173 + Pf6N2E2_4027 0.85 +1.0 4,700,173 + Pf6N2E2_4027 0.85 +0.2 4,700,173 + Pf6N2E2_4027 0.85 +0.6 4,700,174 - Pf6N2E2_4027 0.85 -0.9 4,700,174 - Pf6N2E2_4027 0.85 +0.2 4,700,191 + Pf6N2E2_4027 0.86 -1.5 4,700,192 - Pf6N2E2_4027 0.86 +0.1 4,700,197 + Pf6N2E2_4027 0.86 +0.6 4,700,197 + Pf6N2E2_4027 0.86 +0.4 4,700,197 + Pf6N2E2_4027 0.86 -0.8 4,700,197 + Pf6N2E2_4027 0.86 +0.1 4,700,197 + Pf6N2E2_4027 0.86 -0.0 4,700,198 - Pf6N2E2_4027 0.86 +0.0 4,700,198 - Pf6N2E2_4027 0.86 -0.8 4,700,198 - Pf6N2E2_4027 0.86 -0.4 4,700,262 + -1.3 4,700,262 + +0.1 4,700,262 + +0.0 4,700,262 + +0.1 4,700,263 - -0.8 4,700,404 + -0.6 4,700,404 + +0.3 4,700,404 + -0.6 4,700,404 + -0.3 4,700,405 - -0.1 4,700,405 - +0.9 4,700,480 + +0.4 4,700,480 + -0.1 4,700,481 - +0.7 4,700,481 - +0.8 4,700,481 - +0.1 4,700,614 + Pf6N2E2_4028 0.28 +0.6 4,700,614 + Pf6N2E2_4028 0.28 +0.6 4,700,614 + Pf6N2E2_4028 0.28 -0.4 4,700,614 + Pf6N2E2_4028 0.28 +0.5 4,700,614 + Pf6N2E2_4028 0.28 +0.8 4,700,614 + Pf6N2E2_4028 0.28 -0.4 4,700,615 - Pf6N2E2_4028 0.28 +0.5 4,700,615 - Pf6N2E2_4028 0.28 +1.0 4,700,615 - Pf6N2E2_4028 0.28 +0.4 4,700,615 - Pf6N2E2_4028 0.28 +0.3 4,700,615 - Pf6N2E2_4028 0.28 -0.5 4,700,737 + Pf6N2E2_4028 0.52 +1.0 4,700,799 + Pf6N2E2_4028 0.64 +0.4 4,700,799 + Pf6N2E2_4028 0.64 -1.3 4,700,800 - Pf6N2E2_4028 0.64 -0.1 4,700,800 - Pf6N2E2_4028 0.64 -0.7 4,700,800 - Pf6N2E2_4028 0.64 -0.0 4,700,895 + Pf6N2E2_4028 0.83 +0.5 4,700,895 + Pf6N2E2_4028 0.83 +0.4 4,700,896 - Pf6N2E2_4028 0.83 +0.8 4,700,896 - Pf6N2E2_4028 0.83 +0.3 4,700,896 - Pf6N2E2_4028 0.83 -0.9 4,700,896 - Pf6N2E2_4028 0.83 +0.1 4,700,896 - Pf6N2E2_4028 0.83 +0.5 4,700,896 - Pf6N2E2_4028 0.83 +0.1 4,700,896 - Pf6N2E2_4028 0.83 -0.0 4,700,896 - Pf6N2E2_4028 0.83 +0.0 4,700,896 - Pf6N2E2_4028 0.83 +0.2 4,700,896 - Pf6N2E2_4028 0.83 +0.1 4,700,918 + Pf6N2E2_4028 0.88 +1.2 4,700,919 - Pf6N2E2_4028 0.88 +0.4 4,700,919 - Pf6N2E2_4028 0.88 +0.8 4,700,919 - Pf6N2E2_4028 0.88 -0.2 4,700,972 - -0.7 4,701,068 - +0.1 4,701,068 - +1.2 4,701,068 - +0.8 4,701,356 + Pf6N2E2_4029 0.28 +0.4 4,701,380 + Pf6N2E2_4029 0.30 -0.2 4,701,518 + Pf6N2E2_4029 0.40 +0.7 4,701,519 - Pf6N2E2_4029 0.40 +0.5 4,701,519 - Pf6N2E2_4029 0.40 -0.1 4,701,727 + Pf6N2E2_4029 0.55 +0.3 4,701,810 - Pf6N2E2_4029 0.61 +0.1 4,701,860 + Pf6N2E2_4029 0.65 -2.2 4,701,860 + Pf6N2E2_4029 0.65 +0.5 4,701,861 - Pf6N2E2_4029 0.65 -0.0 4,702,181 + Pf6N2E2_4029 0.89 -0.6 4,702,181 + Pf6N2E2_4029 0.89 -0.1 4,702,182 - Pf6N2E2_4029 0.89 +0.2 4,702,182 - Pf6N2E2_4029 0.89 -0.7 4,702,410 + +0.5 4,702,410 + +0.4 4,702,413 + -0.2 4,702,435 + +1.3 4,702,436 - -1.1 4,702,436 - +0.5 4,702,891 - Pf6N2E2_4030 0.28 -0.8 4,702,891 - Pf6N2E2_4030 0.28 -0.5 4,702,951 - Pf6N2E2_4030 0.31 -0.3 4,702,992 + Pf6N2E2_4030 0.33 +0.2 4,702,992 + Pf6N2E2_4030 0.33 +0.3 4,702,993 - Pf6N2E2_4030 0.33 +0.2 4,703,046 + Pf6N2E2_4030 0.36 +0.6 4,703,046 + Pf6N2E2_4030 0.36 -2.2 4,703,046 + Pf6N2E2_4030 0.36 -0.1 4,703,047 - Pf6N2E2_4030 0.36 -1.1 4,703,047 - Pf6N2E2_4030 0.36 -0.4 4,703,148 + Pf6N2E2_4030 0.41 +0.4 4,703,148 + Pf6N2E2_4030 0.41 -0.1 4,703,148 + Pf6N2E2_4030 0.41 -1.9 4,703,148 + Pf6N2E2_4030 0.41 +0.8 4,703,148 + Pf6N2E2_4030 0.41 +0.4 4,703,149 - Pf6N2E2_4030 0.41 -0.4 4,703,236 - Pf6N2E2_4030 0.45 +0.0 4,703,236 - Pf6N2E2_4030 0.45 +1.0 4,703,236 - Pf6N2E2_4030 0.45 +0.4 4,703,236 - Pf6N2E2_4030 0.45 +0.1 4,703,293 + Pf6N2E2_4030 0.48 -1.8 4,703,293 + Pf6N2E2_4030 0.48 +0.7 4,703,294 - Pf6N2E2_4030 0.48 +0.1 4,703,294 - Pf6N2E2_4030 0.48 -0.9 4,703,314 + Pf6N2E2_4030 0.49 +1.3 4,703,314 + Pf6N2E2_4030 0.49 -0.0
Or see this region's nucleotide sequence