Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3042 and Pf6N2E2_3043 are separated by 43 nucleotides Pf6N2E2_3043 and Pf6N2E2_3044 are separated by 8 nucleotides Pf6N2E2_3044 and Pf6N2E2_3045 are separated by 107 nucleotides
Pf6N2E2_3042 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,526,319 to 3,526,792
_3042
Pf6N2E2_3043 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,526,836 to 3,527,681
_3043
Pf6N2E2_3044 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 3,527,690 to 3,528,802
_3044
Pf6N2E2_3045 - Transcriptional regulator, LysR family, in formaldehyde detoxification operon, at 3,528,910 to 3,529,806
_3045
Position (kb)
3527
3528
3529 Strain fitness (log2 ratio)
-2
-1
0
1 at 3526.828 kb on + strand at 3526.829 kb on - strand at 3526.845 kb on + strand at 3526.845 kb on + strand at 3526.846 kb on - strand at 3526.900 kb on + strand at 3526.900 kb on + strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.913 kb on - strand at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.323 kb on + strand, within Pf6N2E2_3043 at 3527.359 kb on + strand, within Pf6N2E2_3043 at 3527.360 kb on - strand, within Pf6N2E2_3043 at 3527.574 kb on + strand, within Pf6N2E2_3043 at 3527.578 kb on + strand, within Pf6N2E2_3043 at 3527.578 kb on + strand, within Pf6N2E2_3043 at 3527.579 kb on - strand, within Pf6N2E2_3043 at 3527.752 kb on + strand at 3527.786 kb on - strand at 3527.823 kb on + strand, within Pf6N2E2_3044 at 3527.823 kb on + strand, within Pf6N2E2_3044 at 3527.823 kb on + strand, within Pf6N2E2_3044 at 3527.824 kb on - strand, within Pf6N2E2_3044 at 3527.824 kb on - strand, within Pf6N2E2_3044 at 3527.824 kb on - strand, within Pf6N2E2_3044 at 3528.370 kb on - strand, within Pf6N2E2_3044 at 3528.370 kb on - strand, within Pf6N2E2_3044 at 3528.397 kb on - strand, within Pf6N2E2_3044 at 3528.397 kb on - strand, within Pf6N2E2_3044 at 3528.397 kb on - strand, within Pf6N2E2_3044 at 3528.421 kb on - strand, within Pf6N2E2_3044 at 3528.506 kb on - strand, within Pf6N2E2_3044 at 3528.506 kb on - strand, within Pf6N2E2_3044 at 3528.537 kb on + strand, within Pf6N2E2_3044 at 3528.538 kb on - strand, within Pf6N2E2_3044 at 3528.666 kb on + strand, within Pf6N2E2_3044 at 3528.666 kb on + strand, within Pf6N2E2_3044 at 3528.666 kb on + strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.667 kb on - strand, within Pf6N2E2_3044 at 3528.821 kb on - strand at 3528.821 kb on - strand at 3528.841 kb on - strand at 3528.862 kb on + strand at 3529.011 kb on + strand, within Pf6N2E2_3045 at 3529.046 kb on + strand, within Pf6N2E2_3045 at 3529.046 kb on + strand, within Pf6N2E2_3045 at 3529.046 kb on + strand, within Pf6N2E2_3045 at 3529.046 kb on + strand, within Pf6N2E2_3045 at 3529.047 kb on - strand, within Pf6N2E2_3045 at 3529.063 kb on + strand, within Pf6N2E2_3045 at 3529.234 kb on - strand, within Pf6N2E2_3045 at 3529.234 kb on - strand, within Pf6N2E2_3045 at 3529.319 kb on + strand, within Pf6N2E2_3045 at 3529.320 kb on - strand, within Pf6N2E2_3045 at 3529.405 kb on + strand, within Pf6N2E2_3045 at 3529.405 kb on + strand, within Pf6N2E2_3045 at 3529.405 kb on + strand, within Pf6N2E2_3045 at 3529.406 kb on - strand, within Pf6N2E2_3045 at 3529.426 kb on + strand, within Pf6N2E2_3045 at 3529.427 kb on - strand, within Pf6N2E2_3045 at 3529.438 kb on + strand, within Pf6N2E2_3045 at 3529.452 kb on + strand, within Pf6N2E2_3045 at 3529.453 kb on - strand, within Pf6N2E2_3045 at 3529.453 kb on - strand, within Pf6N2E2_3045 at 3529.453 kb on - strand, within Pf6N2E2_3045 at 3529.627 kb on + strand, within Pf6N2E2_3045 at 3529.627 kb on + strand, within Pf6N2E2_3045 at 3529.630 kb on + strand, within Pf6N2E2_3045 at 3529.630 kb on + strand, within Pf6N2E2_3045 at 3529.631 kb on - strand, within Pf6N2E2_3045 at 3529.631 kb on - strand, within Pf6N2E2_3045 at 3529.631 kb on - strand, within Pf6N2E2_3045 at 3529.715 kb on - strand, within Pf6N2E2_3045 at 3529.762 kb on + strand at 3529.762 kb on + strand at 3529.763 kb on - strand at 3529.763 kb on - strand at 3529.763 kb on - strand at 3529.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 3,526,828 + -1.2 3,526,829 - +0.8 3,526,845 + -1.2 3,526,845 + -1.0 3,526,846 - -0.1 3,526,900 + -0.8 3,526,900 + -0.5 3,526,901 - +0.3 3,526,901 - +0.4 3,526,901 - +0.9 3,526,901 - -0.7 3,526,901 - -0.5 3,526,901 - -0.5 3,526,901 - -0.2 3,526,913 - +0.2 3,527,308 + Pf6N2E2_3043 0.56 +0.3 3,527,308 + Pf6N2E2_3043 0.56 -0.0 3,527,308 + Pf6N2E2_3043 0.56 -0.3 3,527,308 + Pf6N2E2_3043 0.56 -1.1 3,527,308 + Pf6N2E2_3043 0.56 +0.4 3,527,308 + Pf6N2E2_3043 0.56 +0.4 3,527,309 - Pf6N2E2_3043 0.56 +0.5 3,527,309 - Pf6N2E2_3043 0.56 +0.3 3,527,309 - Pf6N2E2_3043 0.56 -0.2 3,527,309 - Pf6N2E2_3043 0.56 -0.9 3,527,323 + Pf6N2E2_3043 0.58 +0.1 3,527,359 + Pf6N2E2_3043 0.62 +0.0 3,527,360 - Pf6N2E2_3043 0.62 +0.1 3,527,574 + Pf6N2E2_3043 0.87 -0.4 3,527,578 + Pf6N2E2_3043 0.88 +0.1 3,527,578 + Pf6N2E2_3043 0.88 +0.1 3,527,579 - Pf6N2E2_3043 0.88 +0.4 3,527,752 + -0.7 3,527,786 - +1.1 3,527,823 + Pf6N2E2_3044 0.12 +0.4 3,527,823 + Pf6N2E2_3044 0.12 -1.5 3,527,823 + Pf6N2E2_3044 0.12 +0.6 3,527,824 - Pf6N2E2_3044 0.12 -0.2 3,527,824 - Pf6N2E2_3044 0.12 +0.8 3,527,824 - Pf6N2E2_3044 0.12 +0.8 3,528,370 - Pf6N2E2_3044 0.61 -1.9 3,528,370 - Pf6N2E2_3044 0.61 -0.1 3,528,397 - Pf6N2E2_3044 0.64 -0.7 3,528,397 - Pf6N2E2_3044 0.64 +0.4 3,528,397 - Pf6N2E2_3044 0.64 -0.6 3,528,421 - Pf6N2E2_3044 0.66 -1.7 3,528,506 - Pf6N2E2_3044 0.73 +0.1 3,528,506 - Pf6N2E2_3044 0.73 -0.5 3,528,537 + Pf6N2E2_3044 0.76 -0.6 3,528,538 - Pf6N2E2_3044 0.76 +0.7 3,528,666 + Pf6N2E2_3044 0.88 +0.4 3,528,666 + Pf6N2E2_3044 0.88 -0.5 3,528,666 + Pf6N2E2_3044 0.88 -2.6 3,528,667 - Pf6N2E2_3044 0.88 +0.7 3,528,667 - Pf6N2E2_3044 0.88 +0.5 3,528,667 - Pf6N2E2_3044 0.88 +0.8 3,528,667 - Pf6N2E2_3044 0.88 -2.3 3,528,667 - Pf6N2E2_3044 0.88 +0.5 3,528,667 - Pf6N2E2_3044 0.88 -1.5 3,528,667 - Pf6N2E2_3044 0.88 +1.1 3,528,821 - +0.3 3,528,821 - +0.8 3,528,841 - +0.6 3,528,862 + +0.3 3,529,011 + Pf6N2E2_3045 0.11 -0.2 3,529,046 + Pf6N2E2_3045 0.15 +0.2 3,529,046 + Pf6N2E2_3045 0.15 -0.5 3,529,046 + Pf6N2E2_3045 0.15 +0.2 3,529,046 + Pf6N2E2_3045 0.15 -0.6 3,529,047 - Pf6N2E2_3045 0.15 +0.3 3,529,063 + Pf6N2E2_3045 0.17 +0.3 3,529,234 - Pf6N2E2_3045 0.36 -1.0 3,529,234 - Pf6N2E2_3045 0.36 -0.1 3,529,319 + Pf6N2E2_3045 0.46 +0.1 3,529,320 - Pf6N2E2_3045 0.46 +0.0 3,529,405 + Pf6N2E2_3045 0.55 +0.0 3,529,405 + Pf6N2E2_3045 0.55 -0.3 3,529,405 + Pf6N2E2_3045 0.55 -2.0 3,529,406 - Pf6N2E2_3045 0.55 -0.9 3,529,426 + Pf6N2E2_3045 0.58 +1.4 3,529,427 - Pf6N2E2_3045 0.58 -1.3 3,529,438 + Pf6N2E2_3045 0.59 +0.8 3,529,452 + Pf6N2E2_3045 0.60 +0.5 3,529,453 - Pf6N2E2_3045 0.61 +0.5 3,529,453 - Pf6N2E2_3045 0.61 +0.3 3,529,453 - Pf6N2E2_3045 0.61 +1.1 3,529,627 + Pf6N2E2_3045 0.80 +0.2 3,529,627 + Pf6N2E2_3045 0.80 +0.5 3,529,630 + Pf6N2E2_3045 0.80 +0.8 3,529,630 + Pf6N2E2_3045 0.80 -0.7 3,529,631 - Pf6N2E2_3045 0.80 +0.6 3,529,631 - Pf6N2E2_3045 0.80 +1.5 3,529,631 - Pf6N2E2_3045 0.80 +0.8 3,529,715 - Pf6N2E2_3045 0.90 +0.0 3,529,762 + -0.5 3,529,762 + -0.2 3,529,763 - -0.1 3,529,763 - -0.1 3,529,763 - -0.3 3,529,763 - +0.5
Or see this region's nucleotide sequence