Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5322 and Pf6N2E2_5323 are separated by 186 nucleotides Pf6N2E2_5323 and Pf6N2E2_5324 are separated by 104 nucleotides Pf6N2E2_5324 and Pf6N2E2_5325 are separated by 208 nucleotides
Pf6N2E2_5322 - Predicted transcriptional regulator, at 6,117,437 to 6,117,751
_5322
Pf6N2E2_5323 - FIG00959654: hypothetical protein, at 6,117,938 to 6,118,642
_5323
Pf6N2E2_5324 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,118,747 to 6,119,802
_5324
Pf6N2E2_5325 - Transcriptional regulator, LysR family, at 6,120,011 to 6,120,895
_5325
Position (kb)
6118
6119
6120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6117.914 kb on + strand at 6117.914 kb on + strand at 6117.915 kb on - strand at 6117.915 kb on - strand at 6117.915 kb on - strand at 6117.915 kb on - strand at 6117.939 kb on + strand at 6117.939 kb on + strand at 6117.939 kb on + strand at 6117.940 kb on - strand at 6117.940 kb on - strand at 6118.079 kb on + strand, within Pf6N2E2_5323 at 6118.268 kb on + strand, within Pf6N2E2_5323 at 6118.269 kb on - strand, within Pf6N2E2_5323 at 6118.288 kb on - strand, within Pf6N2E2_5323 at 6118.320 kb on + strand, within Pf6N2E2_5323 at 6118.321 kb on - strand, within Pf6N2E2_5323 at 6118.321 kb on - strand, within Pf6N2E2_5323 at 6118.321 kb on - strand, within Pf6N2E2_5323 at 6118.321 kb on - strand, within Pf6N2E2_5323 at 6118.428 kb on + strand, within Pf6N2E2_5323 at 6118.655 kb on - strand at 6118.709 kb on - strand at 6118.713 kb on + strand at 6118.713 kb on + strand at 6118.713 kb on + strand at 6118.788 kb on - strand at 6118.951 kb on - strand, within Pf6N2E2_5324 at 6119.317 kb on - strand, within Pf6N2E2_5324 at 6119.359 kb on - strand, within Pf6N2E2_5324 at 6119.403 kb on + strand, within Pf6N2E2_5324 at 6119.441 kb on + strand, within Pf6N2E2_5324 at 6119.441 kb on + strand, within Pf6N2E2_5324 at 6119.441 kb on + strand, within Pf6N2E2_5324 at 6119.442 kb on - strand, within Pf6N2E2_5324 at 6119.442 kb on - strand, within Pf6N2E2_5324 at 6119.442 kb on - strand, within Pf6N2E2_5324 at 6119.442 kb on - strand, within Pf6N2E2_5324 at 6119.468 kb on + strand, within Pf6N2E2_5324 at 6119.505 kb on - strand, within Pf6N2E2_5324 at 6119.505 kb on - strand, within Pf6N2E2_5324 at 6119.505 kb on - strand, within Pf6N2E2_5324 at 6119.505 kb on - strand, within Pf6N2E2_5324 at 6119.534 kb on + strand, within Pf6N2E2_5324 at 6119.535 kb on - strand, within Pf6N2E2_5324 at 6119.699 kb on - strand at 6119.736 kb on + strand at 6119.736 kb on + strand at 6119.736 kb on + strand at 6119.736 kb on + strand at 6119.784 kb on + strand at 6119.813 kb on + strand at 6119.813 kb on + strand at 6119.813 kb on + strand at 6119.813 kb on + strand at 6119.813 kb on + strand at 6119.813 kb on + strand at 6119.814 kb on - strand at 6119.814 kb on - strand at 6119.814 kb on - strand at 6119.837 kb on - strand at 6119.837 kb on - strand at 6119.882 kb on + strand at 6120.087 kb on + strand at 6120.120 kb on + strand, within Pf6N2E2_5325 at 6120.120 kb on + strand, within Pf6N2E2_5325 at 6120.120 kb on + strand, within Pf6N2E2_5325 at 6120.121 kb on - strand, within Pf6N2E2_5325 at 6120.121 kb on - strand, within Pf6N2E2_5325 at 6120.121 kb on - strand, within Pf6N2E2_5325 at 6120.121 kb on - strand, within Pf6N2E2_5325 at 6120.405 kb on + strand, within Pf6N2E2_5325 at 6120.405 kb on + strand, within Pf6N2E2_5325 at 6120.405 kb on + strand, within Pf6N2E2_5325 at 6120.406 kb on - strand, within Pf6N2E2_5325 at 6120.406 kb on - strand, within Pf6N2E2_5325 at 6120.406 kb on - strand, within Pf6N2E2_5325 at 6120.435 kb on + strand, within Pf6N2E2_5325 at 6120.435 kb on + strand, within Pf6N2E2_5325 at 6120.435 kb on + strand, within Pf6N2E2_5325 at 6120.435 kb on + strand, within Pf6N2E2_5325 at 6120.436 kb on - strand, within Pf6N2E2_5325 at 6120.718 kb on - strand, within Pf6N2E2_5325 at 6120.718 kb on - strand, within Pf6N2E2_5325 at 6120.718 kb on - strand, within Pf6N2E2_5325 at 6120.720 kb on + strand, within Pf6N2E2_5325 at 6120.720 kb on + strand, within Pf6N2E2_5325 at 6120.720 kb on + strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.721 kb on - strand, within Pf6N2E2_5325 at 6120.760 kb on + strand, within Pf6N2E2_5325 at 6120.776 kb on + strand, within Pf6N2E2_5325
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 6,117,914 + +1.4 6,117,914 + +1.1 6,117,915 - +0.1 6,117,915 - +1.0 6,117,915 - -0.4 6,117,915 - +0.1 6,117,939 + +0.0 6,117,939 + +0.9 6,117,939 + +0.9 6,117,940 - +0.4 6,117,940 - +0.8 6,118,079 + Pf6N2E2_5323 0.20 -0.6 6,118,268 + Pf6N2E2_5323 0.47 -0.1 6,118,269 - Pf6N2E2_5323 0.47 +0.0 6,118,288 - Pf6N2E2_5323 0.50 +0.4 6,118,320 + Pf6N2E2_5323 0.54 +0.3 6,118,321 - Pf6N2E2_5323 0.54 -2.6 6,118,321 - Pf6N2E2_5323 0.54 +0.5 6,118,321 - Pf6N2E2_5323 0.54 -0.3 6,118,321 - Pf6N2E2_5323 0.54 -0.6 6,118,428 + Pf6N2E2_5323 0.70 +0.4 6,118,655 - +0.1 6,118,709 - +0.5 6,118,713 + -0.2 6,118,713 + +0.3 6,118,713 + +0.2 6,118,788 - +0.1 6,118,951 - Pf6N2E2_5324 0.19 +1.1 6,119,317 - Pf6N2E2_5324 0.54 -3.4 6,119,359 - Pf6N2E2_5324 0.58 -0.4 6,119,403 + Pf6N2E2_5324 0.62 +1.7 6,119,441 + Pf6N2E2_5324 0.66 -0.1 6,119,441 + Pf6N2E2_5324 0.66 -1.4 6,119,441 + Pf6N2E2_5324 0.66 +1.0 6,119,442 - Pf6N2E2_5324 0.66 +1.5 6,119,442 - Pf6N2E2_5324 0.66 +1.8 6,119,442 - Pf6N2E2_5324 0.66 +0.1 6,119,442 - Pf6N2E2_5324 0.66 +1.7 6,119,468 + Pf6N2E2_5324 0.68 -0.3 6,119,505 - Pf6N2E2_5324 0.72 +1.4 6,119,505 - Pf6N2E2_5324 0.72 +1.6 6,119,505 - Pf6N2E2_5324 0.72 +1.2 6,119,505 - Pf6N2E2_5324 0.72 +1.1 6,119,534 + Pf6N2E2_5324 0.75 +0.4 6,119,535 - Pf6N2E2_5324 0.75 +0.3 6,119,699 - +0.5 6,119,736 + -1.4 6,119,736 + +0.7 6,119,736 + -0.8 6,119,736 + +0.5 6,119,784 + +0.8 6,119,813 + +1.1 6,119,813 + -0.1 6,119,813 + +1.8 6,119,813 + -0.2 6,119,813 + -0.9 6,119,813 + +1.1 6,119,814 - -0.3 6,119,814 - -0.2 6,119,814 - +0.7 6,119,837 - +0.3 6,119,837 - -0.0 6,119,882 + -0.2 6,120,087 + -0.4 6,120,120 + Pf6N2E2_5325 0.12 -0.7 6,120,120 + Pf6N2E2_5325 0.12 +0.2 6,120,120 + Pf6N2E2_5325 0.12 -0.2 6,120,121 - Pf6N2E2_5325 0.12 +1.2 6,120,121 - Pf6N2E2_5325 0.12 +0.4 6,120,121 - Pf6N2E2_5325 0.12 +0.0 6,120,121 - Pf6N2E2_5325 0.12 +0.3 6,120,405 + Pf6N2E2_5325 0.45 +0.9 6,120,405 + Pf6N2E2_5325 0.45 +1.9 6,120,405 + Pf6N2E2_5325 0.45 -0.1 6,120,406 - Pf6N2E2_5325 0.45 +0.5 6,120,406 - Pf6N2E2_5325 0.45 +0.5 6,120,406 - Pf6N2E2_5325 0.45 -0.6 6,120,435 + Pf6N2E2_5325 0.48 -2.9 6,120,435 + Pf6N2E2_5325 0.48 +0.1 6,120,435 + Pf6N2E2_5325 0.48 -1.8 6,120,435 + Pf6N2E2_5325 0.48 +0.0 6,120,436 - Pf6N2E2_5325 0.48 -0.3 6,120,718 - Pf6N2E2_5325 0.80 +0.2 6,120,718 - Pf6N2E2_5325 0.80 +0.8 6,120,718 - Pf6N2E2_5325 0.80 -0.4 6,120,720 + Pf6N2E2_5325 0.80 -0.1 6,120,720 + Pf6N2E2_5325 0.80 -2.3 6,120,720 + Pf6N2E2_5325 0.80 +0.3 6,120,721 - Pf6N2E2_5325 0.80 -0.3 6,120,721 - Pf6N2E2_5325 0.80 -0.6 6,120,721 - Pf6N2E2_5325 0.80 -0.6 6,120,721 - Pf6N2E2_5325 0.80 -0.9 6,120,721 - Pf6N2E2_5325 0.80 +0.3 6,120,721 - Pf6N2E2_5325 0.80 +0.3 6,120,721 - Pf6N2E2_5325 0.80 -0.4 6,120,760 + Pf6N2E2_5325 0.85 -0.0 6,120,776 + Pf6N2E2_5325 0.86 +1.2
Or see this region's nucleotide sequence