Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5186 and Pf6N2E2_5187 are separated by 82 nucleotides Pf6N2E2_5187 and Pf6N2E2_5188 overlap by 8 nucleotides Pf6N2E2_5188 and Pf6N2E2_5189 are separated by 51 nucleotides Pf6N2E2_5189 and Pf6N2E2_5190 are separated by 132 nucleotides
Pf6N2E2_5186 - FIG00954491: hypothetical protein, at 5,982,488 to 5,983,867
_5186
Pf6N2E2_5187 - Periplasmic protein TonB, links inner and outer membranes, at 5,983,950 to 5,984,729
_5187
Pf6N2E2_5188 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 5,984,722 to 5,985,174
_5188
Pf6N2E2_5189 - Phosphotransferase system, HPr-related proteins, at 5,985,226 to 5,985,630
_5189
Pf6N2E2_5190 - Carbonic anhydrase (EC 4.2.1.1), at 5,985,763 to 5,986,407
_5190
Position (kb)
5984
5985
5986 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5983.793 kb on + strand at 5983.794 kb on - strand at 5983.794 kb on - strand at 5983.794 kb on - strand at 5983.808 kb on + strand at 5983.869 kb on + strand at 5983.870 kb on - strand at 5983.870 kb on - strand at 5983.870 kb on - strand at 5983.908 kb on + strand at 5984.735 kb on + strand at 5984.736 kb on - strand at 5984.788 kb on + strand, within Pf6N2E2_5188 at 5984.788 kb on + strand, within Pf6N2E2_5188 at 5984.797 kb on + strand, within Pf6N2E2_5188 at 5984.798 kb on - strand, within Pf6N2E2_5188 at 5984.846 kb on - strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.005 kb on - strand, within Pf6N2E2_5188 at 5985.005 kb on - strand, within Pf6N2E2_5188 at 5985.070 kb on + strand, within Pf6N2E2_5188 at 5985.070 kb on + strand, within Pf6N2E2_5188 at 5985.112 kb on + strand, within Pf6N2E2_5188 at 5985.295 kb on - strand, within Pf6N2E2_5189 at 5985.643 kb on + strand at 5985.644 kb on - strand at 5985.655 kb on + strand at 5985.655 kb on + strand at 5985.656 kb on - strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.773 kb on + strand at 5985.773 kb on + strand at 5985.773 kb on + strand at 5985.774 kb on - strand at 5985.790 kb on - strand at 5985.871 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5986.141 kb on + strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 5,983,793 + -0.3 5,983,794 - -1.2 5,983,794 - +0.5 5,983,794 - -1.4 5,983,808 + -0.3 5,983,869 + +0.7 5,983,870 - +1.1 5,983,870 - +1.1 5,983,870 - -0.6 5,983,908 + -0.5 5,984,735 + +0.4 5,984,736 - -0.8 5,984,788 + Pf6N2E2_5188 0.15 -2.0 5,984,788 + Pf6N2E2_5188 0.15 -1.9 5,984,797 + Pf6N2E2_5188 0.17 -1.3 5,984,798 - Pf6N2E2_5188 0.17 -0.4 5,984,846 - Pf6N2E2_5188 0.27 +0.8 5,985,004 + Pf6N2E2_5188 0.62 -0.8 5,985,004 + Pf6N2E2_5188 0.62 -1.6 5,985,004 + Pf6N2E2_5188 0.62 -0.1 5,985,005 - Pf6N2E2_5188 0.62 -1.0 5,985,005 - Pf6N2E2_5188 0.62 -1.2 5,985,070 + Pf6N2E2_5188 0.77 +0.2 5,985,070 + Pf6N2E2_5188 0.77 -1.2 5,985,112 + Pf6N2E2_5188 0.86 -1.2 5,985,295 - Pf6N2E2_5189 0.17 +0.1 5,985,643 + +0.0 5,985,644 - -1.1 5,985,655 + +0.6 5,985,655 + +0.8 5,985,656 - -1.8 5,985,742 + -0.1 5,985,742 + -0.6 5,985,742 + -0.2 5,985,742 + +0.8 5,985,773 + -0.7 5,985,773 + -0.8 5,985,773 + -0.5 5,985,774 - -1.3 5,985,790 - -0.0 5,985,871 + Pf6N2E2_5190 0.17 -1.2 5,985,947 + Pf6N2E2_5190 0.29 -1.6 5,985,947 + Pf6N2E2_5190 0.29 -1.0 5,985,947 + Pf6N2E2_5190 0.29 -1.1 5,985,947 + Pf6N2E2_5190 0.29 -1.4 5,985,948 - Pf6N2E2_5190 0.29 -0.5 5,985,948 - Pf6N2E2_5190 0.29 -2.4 5,985,948 - Pf6N2E2_5190 0.29 -0.4 5,985,968 + Pf6N2E2_5190 0.32 -2.6 5,985,968 + Pf6N2E2_5190 0.32 -0.6 5,985,968 + Pf6N2E2_5190 0.32 -2.1 5,985,968 + Pf6N2E2_5190 0.32 -1.8 5,985,968 + Pf6N2E2_5190 0.32 -2.0 5,985,968 + Pf6N2E2_5190 0.32 -0.4 5,985,968 + Pf6N2E2_5190 0.32 -3.2 5,985,969 - Pf6N2E2_5190 0.32 +1.6 5,985,969 - Pf6N2E2_5190 0.32 -3.0 5,985,969 - Pf6N2E2_5190 0.32 -1.2 5,985,969 - Pf6N2E2_5190 0.32 -1.6 5,985,969 - Pf6N2E2_5190 0.32 -2.3 5,985,969 - Pf6N2E2_5190 0.32 -1.5 5,985,969 - Pf6N2E2_5190 0.32 -1.7 5,985,969 - Pf6N2E2_5190 0.32 -2.0 5,986,141 + Pf6N2E2_5190 0.59 +1.0 5,986,142 - Pf6N2E2_5190 0.59 -1.1 5,986,142 - Pf6N2E2_5190 0.59 -1.7 5,986,142 - Pf6N2E2_5190 0.59 -2.8 5,986,142 - Pf6N2E2_5190 0.59 -0.6 5,986,144 + Pf6N2E2_5190 0.59 -0.3 5,986,144 + Pf6N2E2_5190 0.59 -1.0 5,986,144 + Pf6N2E2_5190 0.59 -3.2 5,986,144 + Pf6N2E2_5190 0.59 -2.2 5,986,145 - Pf6N2E2_5190 0.59 -1.2 5,986,145 - Pf6N2E2_5190 0.59 -0.0 5,986,145 - Pf6N2E2_5190 0.59 -1.8
Or see this region's nucleotide sequence