Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5188

Experiment: Glycine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5186 and Pf6N2E2_5187 are separated by 82 nucleotidesPf6N2E2_5187 and Pf6N2E2_5188 overlap by 8 nucleotidesPf6N2E2_5188 and Pf6N2E2_5189 are separated by 51 nucleotidesPf6N2E2_5189 and Pf6N2E2_5190 are separated by 132 nucleotides Pf6N2E2_5186 - FIG00954491: hypothetical protein, at 5,982,488 to 5,983,867 _5186 Pf6N2E2_5187 - Periplasmic protein TonB, links inner and outer membranes, at 5,983,950 to 5,984,729 _5187 Pf6N2E2_5188 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 5,984,722 to 5,985,174 _5188 Pf6N2E2_5189 - Phosphotransferase system, HPr-related proteins, at 5,985,226 to 5,985,630 _5189 Pf6N2E2_5190 - Carbonic anhydrase (EC 4.2.1.1), at 5,985,763 to 5,986,407 _5190 Position (kb) 5984 5985 5986Strain fitness (log2 ratio) -3 -2 -1 0 1at 5983.793 kb on + strandat 5983.794 kb on - strandat 5983.794 kb on - strandat 5983.794 kb on - strandat 5983.808 kb on + strandat 5983.869 kb on + strandat 5983.870 kb on - strandat 5983.870 kb on - strandat 5983.870 kb on - strandat 5983.908 kb on + strandat 5984.735 kb on + strandat 5984.736 kb on - strandat 5984.788 kb on + strand, within Pf6N2E2_5188at 5984.788 kb on + strand, within Pf6N2E2_5188at 5984.797 kb on + strand, within Pf6N2E2_5188at 5984.798 kb on - strand, within Pf6N2E2_5188at 5984.846 kb on - strand, within Pf6N2E2_5188at 5985.004 kb on + strand, within Pf6N2E2_5188at 5985.004 kb on + strand, within Pf6N2E2_5188at 5985.004 kb on + strand, within Pf6N2E2_5188at 5985.005 kb on - strand, within Pf6N2E2_5188at 5985.005 kb on - strand, within Pf6N2E2_5188at 5985.070 kb on + strand, within Pf6N2E2_5188at 5985.070 kb on + strand, within Pf6N2E2_5188at 5985.112 kb on + strand, within Pf6N2E2_5188at 5985.295 kb on - strand, within Pf6N2E2_5189at 5985.643 kb on + strandat 5985.644 kb on - strandat 5985.655 kb on + strandat 5985.655 kb on + strandat 5985.656 kb on - strandat 5985.742 kb on + strandat 5985.742 kb on + strandat 5985.742 kb on + strandat 5985.742 kb on + strandat 5985.773 kb on + strandat 5985.773 kb on + strandat 5985.773 kb on + strandat 5985.774 kb on - strandat 5985.790 kb on - strandat 5985.871 kb on + strand, within Pf6N2E2_5190at 5985.947 kb on + strand, within Pf6N2E2_5190at 5985.947 kb on + strand, within Pf6N2E2_5190at 5985.947 kb on + strand, within Pf6N2E2_5190at 5985.947 kb on + strand, within Pf6N2E2_5190at 5985.948 kb on - strand, within Pf6N2E2_5190at 5985.948 kb on - strand, within Pf6N2E2_5190at 5985.948 kb on - strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.968 kb on + strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5985.969 kb on - strand, within Pf6N2E2_5190at 5986.141 kb on + strand, within Pf6N2E2_5190at 5986.142 kb on - strand, within Pf6N2E2_5190at 5986.142 kb on - strand, within Pf6N2E2_5190at 5986.142 kb on - strand, within Pf6N2E2_5190at 5986.142 kb on - strand, within Pf6N2E2_5190at 5986.144 kb on + strand, within Pf6N2E2_5190at 5986.144 kb on + strand, within Pf6N2E2_5190at 5986.144 kb on + strand, within Pf6N2E2_5190at 5986.144 kb on + strand, within Pf6N2E2_5190at 5986.145 kb on - strand, within Pf6N2E2_5190at 5986.145 kb on - strand, within Pf6N2E2_5190at 5986.145 kb on - strand, within Pf6N2E2_5190

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycine (N)
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5,983,793 + -0.3
5,983,794 - -1.2
5,983,794 - +0.5
5,983,794 - -1.4
5,983,808 + -0.3
5,983,869 + +0.7
5,983,870 - +1.1
5,983,870 - +1.1
5,983,870 - -0.6
5,983,908 + -0.5
5,984,735 + +0.4
5,984,736 - -0.8
5,984,788 + Pf6N2E2_5188 0.15 -2.0
5,984,788 + Pf6N2E2_5188 0.15 -1.9
5,984,797 + Pf6N2E2_5188 0.17 -1.3
5,984,798 - Pf6N2E2_5188 0.17 -0.4
5,984,846 - Pf6N2E2_5188 0.27 +0.8
5,985,004 + Pf6N2E2_5188 0.62 -0.8
5,985,004 + Pf6N2E2_5188 0.62 -1.6
5,985,004 + Pf6N2E2_5188 0.62 -0.1
5,985,005 - Pf6N2E2_5188 0.62 -1.0
5,985,005 - Pf6N2E2_5188 0.62 -1.2
5,985,070 + Pf6N2E2_5188 0.77 +0.2
5,985,070 + Pf6N2E2_5188 0.77 -1.2
5,985,112 + Pf6N2E2_5188 0.86 -1.2
5,985,295 - Pf6N2E2_5189 0.17 +0.1
5,985,643 + +0.0
5,985,644 - -1.1
5,985,655 + +0.6
5,985,655 + +0.8
5,985,656 - -1.8
5,985,742 + -0.1
5,985,742 + -0.6
5,985,742 + -0.2
5,985,742 + +0.8
5,985,773 + -0.7
5,985,773 + -0.8
5,985,773 + -0.5
5,985,774 - -1.3
5,985,790 - -0.0
5,985,871 + Pf6N2E2_5190 0.17 -1.2
5,985,947 + Pf6N2E2_5190 0.29 -1.6
5,985,947 + Pf6N2E2_5190 0.29 -1.0
5,985,947 + Pf6N2E2_5190 0.29 -1.1
5,985,947 + Pf6N2E2_5190 0.29 -1.4
5,985,948 - Pf6N2E2_5190 0.29 -0.5
5,985,948 - Pf6N2E2_5190 0.29 -2.4
5,985,948 - Pf6N2E2_5190 0.29 -0.4
5,985,968 + Pf6N2E2_5190 0.32 -2.6
5,985,968 + Pf6N2E2_5190 0.32 -0.6
5,985,968 + Pf6N2E2_5190 0.32 -2.1
5,985,968 + Pf6N2E2_5190 0.32 -1.8
5,985,968 + Pf6N2E2_5190 0.32 -2.0
5,985,968 + Pf6N2E2_5190 0.32 -0.4
5,985,968 + Pf6N2E2_5190 0.32 -3.2
5,985,969 - Pf6N2E2_5190 0.32 +1.6
5,985,969 - Pf6N2E2_5190 0.32 -3.0
5,985,969 - Pf6N2E2_5190 0.32 -1.2
5,985,969 - Pf6N2E2_5190 0.32 -1.6
5,985,969 - Pf6N2E2_5190 0.32 -2.3
5,985,969 - Pf6N2E2_5190 0.32 -1.5
5,985,969 - Pf6N2E2_5190 0.32 -1.7
5,985,969 - Pf6N2E2_5190 0.32 -2.0
5,986,141 + Pf6N2E2_5190 0.59 +1.0
5,986,142 - Pf6N2E2_5190 0.59 -1.1
5,986,142 - Pf6N2E2_5190 0.59 -1.7
5,986,142 - Pf6N2E2_5190 0.59 -2.8
5,986,142 - Pf6N2E2_5190 0.59 -0.6
5,986,144 + Pf6N2E2_5190 0.59 -0.3
5,986,144 + Pf6N2E2_5190 0.59 -1.0
5,986,144 + Pf6N2E2_5190 0.59 -3.2
5,986,144 + Pf6N2E2_5190 0.59 -2.2
5,986,145 - Pf6N2E2_5190 0.59 -1.2
5,986,145 - Pf6N2E2_5190 0.59 -0.0
5,986,145 - Pf6N2E2_5190 0.59 -1.8

Or see this region's nucleotide sequence