Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4685 and Pf6N2E2_4686 are separated by 574 nucleotides Pf6N2E2_4686 and Pf6N2E2_4687 are separated by 144 nucleotides Pf6N2E2_4687 and Pf6N2E2_4688 are separated by 300 nucleotides
Pf6N2E2_4685 - L-serine dehydratase (EC 4.3.1.17), at 5,418,363 to 5,419,739
_4685
Pf6N2E2_4686 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,420,314 to 5,421,417
_4686
Pf6N2E2_4687 - FIG00953780: hypothetical protein, at 5,421,562 to 5,421,825
_4687
Pf6N2E2_4688 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 5,422,126 to 5,423,289
_4688
Position (kb) 5420
5421
5422 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5419.317 kb on + strand, within Pf6N2E2_4685 at 5419.318 kb on - strand, within Pf6N2E2_4685 at 5419.318 kb on - strand, within Pf6N2E2_4685 at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.690 kb on - strand at 5419.690 kb on - strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.692 kb on - strand at 5419.887 kb on - strand at 5419.887 kb on - strand at 5419.898 kb on + strand at 5419.899 kb on - strand at 5419.899 kb on - strand at 5419.933 kb on + strand at 5419.934 kb on - strand at 5420.017 kb on + strand at 5420.017 kb on + strand at 5420.017 kb on + strand at 5420.018 kb on - strand at 5420.018 kb on - strand at 5420.059 kb on - strand at 5420.130 kb on + strand at 5420.130 kb on + strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.141 kb on + strand at 5420.142 kb on - strand at 5420.171 kb on + strand at 5420.172 kb on - strand at 5420.172 kb on - strand at 5420.172 kb on - strand at 5420.217 kb on + strand at 5420.217 kb on + strand at 5420.217 kb on + strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.252 kb on + strand at 5420.307 kb on + strand at 5420.307 kb on + strand at 5420.417 kb on + strand at 5420.426 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.461 kb on + strand, within Pf6N2E2_4686 at 5420.462 kb on - strand, within Pf6N2E2_4686 at 5420.562 kb on + strand, within Pf6N2E2_4686 at 5420.599 kb on - strand, within Pf6N2E2_4686 at 5420.781 kb on + strand, within Pf6N2E2_4686 at 5420.915 kb on - strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.059 kb on - strand, within Pf6N2E2_4686 at 5421.104 kb on - strand, within Pf6N2E2_4686 at 5421.104 kb on - strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.128 kb on - strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.207 kb on - strand, within Pf6N2E2_4686 at 5421.254 kb on - strand, within Pf6N2E2_4686 at 5421.385 kb on - strand at 5421.415 kb on + strand at 5421.416 kb on - strand at 5421.479 kb on + strand at 5421.480 kb on - strand at 5421.488 kb on + strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.642 kb on + strand, within Pf6N2E2_4687 at 5421.643 kb on - strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.757 kb on + strand, within Pf6N2E2_4687 at 5421.818 kb on + strand at 5421.818 kb on + strand at 5421.819 kb on - strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.923 kb on - strand at 5421.923 kb on - strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.283 kb on - strand, within Pf6N2E2_4688
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 5,419,317 + Pf6N2E2_4685 0.69 +0.5 5,419,318 - Pf6N2E2_4685 0.69 +1.0 5,419,318 - Pf6N2E2_4685 0.69 -0.5 5,419,689 + +1.0 5,419,689 + +1.2 5,419,689 + +1.0 5,419,689 + +0.7 5,419,689 + +0.0 5,419,690 - -0.2 5,419,690 - -0.1 5,419,691 + -0.1 5,419,691 + +1.1 5,419,691 + +0.4 5,419,691 + +0.4 5,419,691 + +1.1 5,419,692 - -0.5 5,419,887 - -0.3 5,419,887 - -1.0 5,419,898 + +0.3 5,419,899 - -0.8 5,419,899 - -0.4 5,419,933 + +2.0 5,419,934 - +0.1 5,420,017 + +2.2 5,420,017 + +0.6 5,420,017 + +1.6 5,420,018 - -0.1 5,420,018 - +0.9 5,420,059 - -0.1 5,420,130 + +2.1 5,420,130 + +2.0 5,420,131 - -2.0 5,420,131 - -1.1 5,420,131 - -0.1 5,420,131 - +0.4 5,420,131 - +0.8 5,420,141 + +3.0 5,420,142 - -1.3 5,420,171 + +1.4 5,420,172 - -0.9 5,420,172 - -0.1 5,420,172 - -1.2 5,420,217 + +1.2 5,420,217 + +0.7 5,420,217 + -0.5 5,420,218 - -0.9 5,420,218 - +0.8 5,420,218 - -2.4 5,420,218 - -1.1 5,420,252 + +1.1 5,420,307 + -0.1 5,420,307 + -2.1 5,420,417 + -1.2 5,420,426 + Pf6N2E2_4686 0.10 -1.1 5,420,459 + Pf6N2E2_4686 0.13 -1.0 5,420,459 + Pf6N2E2_4686 0.13 -0.8 5,420,459 + Pf6N2E2_4686 0.13 -0.2 5,420,459 + Pf6N2E2_4686 0.13 -1.2 5,420,460 - Pf6N2E2_4686 0.13 -1.1 5,420,460 - Pf6N2E2_4686 0.13 +0.2 5,420,460 - Pf6N2E2_4686 0.13 -0.1 5,420,460 - Pf6N2E2_4686 0.13 -0.7 5,420,461 + Pf6N2E2_4686 0.13 -0.5 5,420,462 - Pf6N2E2_4686 0.13 -2.4 5,420,562 + Pf6N2E2_4686 0.22 -0.2 5,420,599 - Pf6N2E2_4686 0.26 -1.8 5,420,781 + Pf6N2E2_4686 0.42 -0.5 5,420,915 - Pf6N2E2_4686 0.54 -0.5 5,421,058 + Pf6N2E2_4686 0.67 -0.6 5,421,058 + Pf6N2E2_4686 0.67 -2.1 5,421,058 + Pf6N2E2_4686 0.67 -0.4 5,421,058 + Pf6N2E2_4686 0.67 -0.7 5,421,059 - Pf6N2E2_4686 0.67 +0.4 5,421,104 - Pf6N2E2_4686 0.72 -1.6 5,421,104 - Pf6N2E2_4686 0.72 -0.9 5,421,127 + Pf6N2E2_4686 0.74 -1.4 5,421,127 + Pf6N2E2_4686 0.74 -1.7 5,421,128 - Pf6N2E2_4686 0.74 +0.2 5,421,130 + Pf6N2E2_4686 0.74 -1.5 5,421,130 + Pf6N2E2_4686 0.74 -0.4 5,421,130 + Pf6N2E2_4686 0.74 -0.2 5,421,130 + Pf6N2E2_4686 0.74 -1.0 5,421,130 + Pf6N2E2_4686 0.74 -1.3 5,421,130 + Pf6N2E2_4686 0.74 -0.4 5,421,131 - Pf6N2E2_4686 0.74 -1.6 5,421,131 - Pf6N2E2_4686 0.74 -1.1 5,421,207 - Pf6N2E2_4686 0.81 -0.4 5,421,254 - Pf6N2E2_4686 0.85 -1.4 5,421,385 - +0.5 5,421,415 + -0.5 5,421,416 - -1.2 5,421,479 + -1.3 5,421,480 - +0.1 5,421,488 + -0.3 5,421,489 - +0.3 5,421,489 - +0.0 5,421,489 - +0.4 5,421,489 - +1.2 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 -0.2 5,421,613 - Pf6N2E2_4687 0.19 -0.5 5,421,613 - Pf6N2E2_4687 0.19 +0.1 5,421,613 - Pf6N2E2_4687 0.19 +0.1 5,421,642 + Pf6N2E2_4687 0.30 -0.2 5,421,643 - Pf6N2E2_4687 0.31 +0.2 5,421,659 + Pf6N2E2_4687 0.37 +0.2 5,421,659 + Pf6N2E2_4687 0.37 -1.2 5,421,659 + Pf6N2E2_4687 0.37 +0.0 5,421,659 + Pf6N2E2_4687 0.37 -0.1 5,421,659 + Pf6N2E2_4687 0.37 -0.5 5,421,660 - Pf6N2E2_4687 0.37 +0.4 5,421,660 - Pf6N2E2_4687 0.37 -0.3 5,421,660 - Pf6N2E2_4687 0.37 -1.6 5,421,660 - Pf6N2E2_4687 0.37 -0.0 5,421,660 - Pf6N2E2_4687 0.37 +0.3 5,421,660 - Pf6N2E2_4687 0.37 -2.1 5,421,660 - Pf6N2E2_4687 0.37 -0.9 5,421,757 + Pf6N2E2_4687 0.74 -0.8 5,421,818 + +0.6 5,421,818 + -1.2 5,421,819 - +0.8 5,421,922 + -0.2 5,421,922 + -0.4 5,421,922 + -0.6 5,421,923 - +0.7 5,421,923 - -0.0 5,422,175 + +0.1 5,422,175 + +1.0 5,422,175 + -0.7 5,422,175 + -0.1 5,422,175 + -1.0 5,422,175 + -0.3 5,422,175 + +0.2 5,422,175 + -0.7 5,422,175 + +0.3 5,422,175 + -1.7 5,422,175 + -0.5 5,422,176 - -1.1 5,422,176 - -1.1 5,422,176 - -0.8 5,422,176 - -0.0 5,422,176 - -1.4 5,422,176 - -0.4 5,422,176 - -0.9 5,422,176 - +0.5 5,422,176 - +0.4 5,422,176 - -0.4 5,422,176 - -0.6 5,422,176 - +0.7 5,422,176 - +0.6 5,422,283 - Pf6N2E2_4688 0.13 +1.5
Or see this region's nucleotide sequence