Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2894 and Pf6N2E2_2895 are separated by 240 nucleotides Pf6N2E2_2895 and Pf6N2E2_2896 overlap by 11 nucleotides Pf6N2E2_2896 and Pf6N2E2_2897 are separated by 184 nucleotides
Pf6N2E2_2894 - N-acylglucosamine 2-epimerase (EC 5.1.3.8), at 3,382,055 to 3,383,314
_2894
Pf6N2E2_2895 - Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster, at 3,383,555 to 3,385,024
_2895
Pf6N2E2_2896 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 3,385,014 to 3,385,745
_2896
Pf6N2E2_2897 - Glucokinase (EC 2.7.1.2), at 3,385,930 to 3,386,889
_2897
Position (kb)
3383
3384
3385
3386 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3382.605 kb on + strand, within Pf6N2E2_2894 at 3383.323 kb on - strand at 3383.323 kb on - strand at 3383.353 kb on + strand at 3383.354 kb on - strand at 3383.354 kb on - strand at 3383.354 kb on - strand at 3383.395 kb on + strand at 3383.395 kb on + strand at 3383.395 kb on + strand at 3383.395 kb on + strand at 3383.396 kb on - strand at 3383.396 kb on - strand at 3383.396 kb on - strand at 3383.396 kb on - strand at 3383.415 kb on + strand at 3383.415 kb on + strand at 3383.416 kb on - strand at 3383.416 kb on - strand at 3383.416 kb on - strand at 3383.416 kb on - strand at 3383.483 kb on + strand at 3383.483 kb on + strand at 3383.556 kb on + strand at 3383.852 kb on - strand, within Pf6N2E2_2895 at 3384.162 kb on - strand, within Pf6N2E2_2895 at 3384.162 kb on - strand, within Pf6N2E2_2895 at 3384.162 kb on - strand, within Pf6N2E2_2895 at 3384.429 kb on + strand, within Pf6N2E2_2895 at 3384.429 kb on + strand, within Pf6N2E2_2895 at 3384.429 kb on + strand, within Pf6N2E2_2895 at 3384.456 kb on + strand, within Pf6N2E2_2895 at 3384.456 kb on + strand, within Pf6N2E2_2895 at 3384.456 kb on + strand, within Pf6N2E2_2895 at 3384.457 kb on - strand, within Pf6N2E2_2895 at 3384.457 kb on - strand, within Pf6N2E2_2895 at 3384.457 kb on - strand, within Pf6N2E2_2895 at 3384.531 kb on + strand, within Pf6N2E2_2895 at 3384.532 kb on - strand, within Pf6N2E2_2895 at 3384.532 kb on - strand, within Pf6N2E2_2895 at 3384.532 kb on - strand, within Pf6N2E2_2895 at 3384.532 kb on - strand, within Pf6N2E2_2895 at 3384.615 kb on + strand, within Pf6N2E2_2895 at 3384.617 kb on + strand, within Pf6N2E2_2895 at 3384.617 kb on + strand, within Pf6N2E2_2895 at 3384.618 kb on - strand, within Pf6N2E2_2895 at 3384.813 kb on + strand, within Pf6N2E2_2895 at 3384.813 kb on + strand, within Pf6N2E2_2895 at 3384.814 kb on - strand, within Pf6N2E2_2895 at 3384.814 kb on - strand, within Pf6N2E2_2895 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.433 kb on - strand, within Pf6N2E2_2896 at 3385.545 kb on + strand, within Pf6N2E2_2896 at 3385.545 kb on + strand, within Pf6N2E2_2896 at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.676 kb on - strand at 3385.676 kb on - strand at 3385.779 kb on + strand at 3385.780 kb on - strand at 3385.799 kb on + strand at 3385.800 kb on - strand at 3385.800 kb on - strand at 3385.810 kb on + strand at 3385.811 kb on - strand at 3385.913 kb on - strand at 3385.913 kb on - strand at 3385.913 kb on - strand at 3385.922 kb on - strand at 3386.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 3,382,605 + Pf6N2E2_2894 0.44 -0.9 3,383,323 - +0.4 3,383,323 - +0.5 3,383,353 + -0.3 3,383,354 - +0.8 3,383,354 - -0.2 3,383,354 - +0.7 3,383,395 + +0.3 3,383,395 + +0.6 3,383,395 + +0.7 3,383,395 + +0.8 3,383,396 - -0.5 3,383,396 - -0.1 3,383,396 - +1.4 3,383,396 - +0.0 3,383,415 + +0.2 3,383,415 + +0.9 3,383,416 - +0.2 3,383,416 - +0.8 3,383,416 - -0.0 3,383,416 - -0.1 3,383,483 + +0.2 3,383,483 + +0.4 3,383,556 + -0.4 3,383,852 - Pf6N2E2_2895 0.20 -2.0 3,384,162 - Pf6N2E2_2895 0.41 -2.8 3,384,162 - Pf6N2E2_2895 0.41 -3.0 3,384,162 - Pf6N2E2_2895 0.41 -1.9 3,384,429 + Pf6N2E2_2895 0.59 -1.8 3,384,429 + Pf6N2E2_2895 0.59 -2.9 3,384,429 + Pf6N2E2_2895 0.59 -0.3 3,384,456 + Pf6N2E2_2895 0.61 -3.6 3,384,456 + Pf6N2E2_2895 0.61 -2.1 3,384,456 + Pf6N2E2_2895 0.61 -1.2 3,384,457 - Pf6N2E2_2895 0.61 -1.3 3,384,457 - Pf6N2E2_2895 0.61 -2.6 3,384,457 - Pf6N2E2_2895 0.61 -1.1 3,384,531 + Pf6N2E2_2895 0.66 -2.4 3,384,532 - Pf6N2E2_2895 0.66 -2.4 3,384,532 - Pf6N2E2_2895 0.66 +0.4 3,384,532 - Pf6N2E2_2895 0.66 -2.5 3,384,532 - Pf6N2E2_2895 0.66 -1.5 3,384,615 + Pf6N2E2_2895 0.72 -2.5 3,384,617 + Pf6N2E2_2895 0.72 -1.3 3,384,617 + Pf6N2E2_2895 0.72 -1.3 3,384,618 - Pf6N2E2_2895 0.72 -0.4 3,384,813 + Pf6N2E2_2895 0.86 -2.2 3,384,813 + Pf6N2E2_2895 0.86 -1.8 3,384,814 - Pf6N2E2_2895 0.86 -2.5 3,384,814 - Pf6N2E2_2895 0.86 -2.9 3,385,432 + Pf6N2E2_2896 0.57 -3.4 3,385,432 + Pf6N2E2_2896 0.57 -1.7 3,385,432 + Pf6N2E2_2896 0.57 -1.9 3,385,432 + Pf6N2E2_2896 0.57 -1.4 3,385,433 - Pf6N2E2_2896 0.57 -0.2 3,385,545 + Pf6N2E2_2896 0.73 -3.1 3,385,545 + Pf6N2E2_2896 0.73 -3.3 3,385,675 + -2.7 3,385,675 + -2.3 3,385,675 + -3.1 3,385,675 + -2.1 3,385,675 + +0.1 3,385,675 + -3.1 3,385,675 + -1.4 3,385,676 - -1.8 3,385,676 - -1.4 3,385,779 + -2.6 3,385,780 - -0.7 3,385,799 + -3.5 3,385,800 - +0.1 3,385,800 - +0.4 3,385,810 + -1.4 3,385,811 - -0.3 3,385,913 - -0.1 3,385,913 - +0.2 3,385,913 - +0.1 3,385,922 - +0.2 3,386,022 - -5.3
Or see this region's nucleotide sequence