Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_236 and Pf6N2E2_237 are separated by 291 nucleotides Pf6N2E2_237 and Pf6N2E2_238 are separated by 219 nucleotides Pf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides Pf6N2E2_239 and Pf6N2E2_240 are separated by 171 nucleotides
Pf6N2E2_236 - putative transcriptional regulator protein, at 298,600 to 299,253
_236
Pf6N2E2_237 - hypothetical protein, at 299,545 to 299,757
_237
Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096
_238
Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831
_239
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Position (kb) 299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 299.089 kb on + strand, within Pf6N2E2_236 at 299.090 kb on - strand, within Pf6N2E2_236 at 299.116 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.165 kb on + strand, within Pf6N2E2_236 at 299.272 kb on + strand at 299.272 kb on + strand at 299.301 kb on - strand at 299.307 kb on + strand at 299.392 kb on + strand at 299.393 kb on - strand at 299.413 kb on + strand at 299.414 kb on - strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.538 kb on + strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.545 kb on - strand at 299.545 kb on - strand at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.747 kb on - strand at 299.786 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.813 kb on - strand at 299.813 kb on - strand at 299.871 kb on - strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.896 kb on + strand at 299.978 kb on + strand at 299.979 kb on - strand at 299.992 kb on + strand, within Pf6N2E2_238 at 299.992 kb on + strand, within Pf6N2E2_238 at 300.010 kb on - strand, within Pf6N2E2_238 at 300.172 kb on + strand at 300.183 kb on + strand at 300.184 kb on - strand at 300.184 kb on - strand at 300.295 kb on + strand, within Pf6N2E2_239 at 300.295 kb on + strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.559 kb on + strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.709 kb on - strand, within Pf6N2E2_239 at 300.749 kb on - strand, within Pf6N2E2_239 at 300.764 kb on - strand at 300.764 kb on - strand at 300.773 kb on + strand at 300.830 kb on - strand at 300.835 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.845 kb on - strand at 300.845 kb on - strand at 300.894 kb on + strand at 300.894 kb on + strand at 300.960 kb on - strand at 300.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 299,089 + Pf6N2E2_236 0.75 +0.5 299,090 - Pf6N2E2_236 0.75 +0.7 299,116 + Pf6N2E2_236 0.79 +0.4 299,129 + Pf6N2E2_236 0.81 +1.3 299,129 + Pf6N2E2_236 0.81 +0.3 299,129 + Pf6N2E2_236 0.81 -0.5 299,130 - Pf6N2E2_236 0.81 -1.1 299,130 - Pf6N2E2_236 0.81 -0.2 299,165 + Pf6N2E2_236 0.86 -0.5 299,272 + +0.5 299,272 + +0.2 299,301 - -0.6 299,307 + -0.2 299,392 + -0.1 299,393 - +0.7 299,413 + -1.5 299,414 - -0.6 299,491 + +0.4 299,491 + +1.8 299,491 + -0.3 299,492 - +2.0 299,492 - -0.3 299,492 - +1.4 299,492 - +0.1 299,492 - -2.8 299,492 - +0.3 299,492 - +0.4 299,492 - +0.5 299,492 - -0.4 299,538 + -0.4 299,539 - -3.1 299,539 - +0.7 299,539 - -2.4 299,544 + -1.2 299,544 + +0.3 299,544 + -1.3 299,544 + +0.5 299,545 - +0.3 299,545 - +0.0 299,591 - Pf6N2E2_237 0.22 +0.5 299,591 - Pf6N2E2_237 0.22 +0.7 299,591 - Pf6N2E2_237 0.22 -1.3 299,591 - Pf6N2E2_237 0.22 +0.6 299,747 - +0.5 299,786 - -0.0 299,810 - +0.9 299,810 - +0.6 299,810 - -1.3 299,810 - +1.0 299,810 - -1.3 299,813 - +0.7 299,813 - +0.4 299,871 - -2.0 299,880 + +0.6 299,880 + +0.0 299,880 + +0.9 299,881 - +0.4 299,881 - +0.6 299,881 - +0.1 299,896 + -1.0 299,978 + -1.0 299,979 - -0.5 299,992 + Pf6N2E2_238 0.12 -0.8 299,992 + Pf6N2E2_238 0.12 -0.1 300,010 - Pf6N2E2_238 0.28 -1.1 300,172 + -1.4 300,183 + +2.3 300,184 - -1.0 300,184 - +1.2 300,295 + Pf6N2E2_239 0.24 +1.2 300,295 + Pf6N2E2_239 0.24 +2.6 300,296 - Pf6N2E2_239 0.24 +0.5 300,296 - Pf6N2E2_239 0.24 -0.4 300,297 + Pf6N2E2_239 0.24 +0.5 300,297 + Pf6N2E2_239 0.24 +1.0 300,297 + Pf6N2E2_239 0.24 +0.2 300,298 - Pf6N2E2_239 0.24 +1.9 300,298 - Pf6N2E2_239 0.24 +0.5 300,298 - Pf6N2E2_239 0.24 -0.4 300,298 - Pf6N2E2_239 0.24 +0.3 300,298 - Pf6N2E2_239 0.24 -1.1 300,298 - Pf6N2E2_239 0.24 +0.9 300,298 - Pf6N2E2_239 0.24 +2.1 300,482 - Pf6N2E2_239 0.50 +0.2 300,482 - Pf6N2E2_239 0.50 +0.8 300,506 - Pf6N2E2_239 0.54 +0.6 300,506 - Pf6N2E2_239 0.54 +0.6 300,559 + Pf6N2E2_239 0.61 +1.5 300,595 - Pf6N2E2_239 0.66 +0.5 300,595 - Pf6N2E2_239 0.66 +0.0 300,707 - Pf6N2E2_239 0.82 -0.2 300,707 - Pf6N2E2_239 0.82 +0.8 300,709 - Pf6N2E2_239 0.82 -0.5 300,749 - Pf6N2E2_239 0.88 +1.0 300,764 - +0.4 300,764 - -0.1 300,773 + +1.8 300,830 - -0.2 300,835 + -0.7 300,844 + +0.9 300,844 + +0.2 300,844 + +1.0 300,844 + -0.6 300,845 - +1.0 300,845 - +0.0 300,894 + -0.4 300,894 + -0.2 300,960 - +2.4 300,960 - -0.2
Or see this region's nucleotide sequence