Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2116 and Pf6N2E2_2117 are separated by 196 nucleotides Pf6N2E2_2117 and Pf6N2E2_2118 are separated by 11 nucleotides
Pf6N2E2_2116 - Glucarate dehydratase (EC 4.2.1.40), at 2,513,809 to 2,515,152
_2116
Pf6N2E2_2117 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 2,515,349 to 2,517,583
_2117
Pf6N2E2_2118 - Trehalose synthase (EC 5.4.99.16), at 2,517,595 to 2,520,939
_2118
Position (kb)
2515
2516
2517
2518 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2514.578 kb on + strand, within Pf6N2E2_2116 at 2514.578 kb on + strand, within Pf6N2E2_2116 at 2514.579 kb on - strand, within Pf6N2E2_2116 at 2514.587 kb on - strand, within Pf6N2E2_2116 at 2515.051 kb on + strand at 2515.051 kb on + strand at 2515.051 kb on + strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.068 kb on - strand at 2515.208 kb on - strand at 2515.208 kb on - strand at 2515.343 kb on + strand at 2515.350 kb on + strand at 2515.461 kb on + strand at 2515.462 kb on - strand at 2515.462 kb on - strand at 2515.462 kb on - strand at 2515.476 kb on + strand at 2515.476 kb on + strand at 2515.477 kb on - strand at 2515.608 kb on - strand, within Pf6N2E2_2117 at 2515.617 kb on + strand, within Pf6N2E2_2117 at 2515.618 kb on - strand, within Pf6N2E2_2117 at 2515.768 kb on - strand, within Pf6N2E2_2117 at 2515.869 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2516.052 kb on + strand, within Pf6N2E2_2117 at 2516.052 kb on + strand, within Pf6N2E2_2117 at 2516.053 kb on - strand, within Pf6N2E2_2117 at 2516.053 kb on - strand, within Pf6N2E2_2117 at 2516.053 kb on - strand, within Pf6N2E2_2117 at 2516.091 kb on + strand, within Pf6N2E2_2117 at 2516.092 kb on - strand, within Pf6N2E2_2117 at 2516.092 kb on - strand, within Pf6N2E2_2117 at 2516.092 kb on - strand, within Pf6N2E2_2117 at 2516.254 kb on + strand, within Pf6N2E2_2117 at 2516.255 kb on - strand, within Pf6N2E2_2117 at 2516.292 kb on + strand, within Pf6N2E2_2117 at 2516.403 kb on + strand, within Pf6N2E2_2117 at 2516.451 kb on + strand, within Pf6N2E2_2117 at 2516.458 kb on - strand, within Pf6N2E2_2117 at 2516.704 kb on - strand, within Pf6N2E2_2117 at 2516.704 kb on - strand, within Pf6N2E2_2117 at 2516.739 kb on + strand, within Pf6N2E2_2117 at 2516.739 kb on + strand, within Pf6N2E2_2117 at 2516.739 kb on + strand, within Pf6N2E2_2117 at 2516.740 kb on - strand, within Pf6N2E2_2117 at 2516.740 kb on - strand, within Pf6N2E2_2117 at 2516.802 kb on + strand, within Pf6N2E2_2117 at 2516.802 kb on + strand, within Pf6N2E2_2117 at 2516.982 kb on + strand at 2516.983 kb on - strand, within Pf6N2E2_2117 at 2516.983 kb on - strand, within Pf6N2E2_2117 at 2516.988 kb on + strand, within Pf6N2E2_2117 at 2516.988 kb on + strand, within Pf6N2E2_2117 at 2516.989 kb on - strand, within Pf6N2E2_2117 at 2516.989 kb on - strand, within Pf6N2E2_2117 at 2517.020 kb on + strand, within Pf6N2E2_2117 at 2517.020 kb on + strand, within Pf6N2E2_2117 at 2517.020 kb on + strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.219 kb on + strand, within Pf6N2E2_2117 at 2517.219 kb on + strand, within Pf6N2E2_2117 at 2517.220 kb on - strand, within Pf6N2E2_2117 at 2517.255 kb on + strand, within Pf6N2E2_2117 at 2517.255 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.307 kb on - strand, within Pf6N2E2_2117 at 2517.307 kb on - strand, within Pf6N2E2_2117 at 2517.699 kb on - strand at 2517.711 kb on - strand at 2517.786 kb on + strand at 2517.786 kb on + strand at 2517.787 kb on - strand at 2517.804 kb on - strand at 2517.923 kb on + strand at 2517.924 kb on - strand at 2517.924 kb on - strand at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.959 kb on + strand, within Pf6N2E2_2118 at 2517.959 kb on + strand, within Pf6N2E2_2118 at 2517.959 kb on + strand, within Pf6N2E2_2118 at 2517.960 kb on - strand, within Pf6N2E2_2118 at 2517.960 kb on - strand, within Pf6N2E2_2118 at 2518.049 kb on - strand, within Pf6N2E2_2118 at 2518.321 kb on + strand, within Pf6N2E2_2118 at 2518.391 kb on + strand, within Pf6N2E2_2118 at 2518.391 kb on + strand, within Pf6N2E2_2118 at 2518.392 kb on - strand, within Pf6N2E2_2118 at 2518.493 kb on + strand, within Pf6N2E2_2118 at 2518.494 kb on - strand, within Pf6N2E2_2118
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 2,514,578 + Pf6N2E2_2116 0.57 +0.5 2,514,578 + Pf6N2E2_2116 0.57 -0.1 2,514,579 - Pf6N2E2_2116 0.57 -0.1 2,514,587 - Pf6N2E2_2116 0.58 -0.8 2,515,051 + +0.6 2,515,051 + -0.5 2,515,051 + +0.1 2,515,052 - -0.3 2,515,052 - +2.3 2,515,052 - -0.5 2,515,052 - -0.8 2,515,052 - -2.2 2,515,052 - -0.3 2,515,052 - +0.0 2,515,068 - -0.2 2,515,208 - +1.0 2,515,208 - +1.2 2,515,343 + -2.1 2,515,350 + +0.6 2,515,461 + -2.5 2,515,462 - -3.4 2,515,462 - -1.0 2,515,462 - -2.0 2,515,476 + -1.1 2,515,476 + +0.0 2,515,477 - -1.5 2,515,608 - Pf6N2E2_2117 0.12 -0.7 2,515,617 + Pf6N2E2_2117 0.12 -2.8 2,515,618 - Pf6N2E2_2117 0.12 -1.2 2,515,768 - Pf6N2E2_2117 0.19 -1.1 2,515,869 + Pf6N2E2_2117 0.23 -1.6 2,515,989 + Pf6N2E2_2117 0.29 -2.0 2,515,989 + Pf6N2E2_2117 0.29 -1.6 2,515,989 + Pf6N2E2_2117 0.29 -1.3 2,515,989 + Pf6N2E2_2117 0.29 -0.5 2,515,989 + Pf6N2E2_2117 0.29 -1.1 2,515,989 + Pf6N2E2_2117 0.29 -2.2 2,515,989 + Pf6N2E2_2117 0.29 -0.2 2,515,989 + Pf6N2E2_2117 0.29 -1.3 2,515,990 - Pf6N2E2_2117 0.29 -1.0 2,515,990 - Pf6N2E2_2117 0.29 -1.5 2,515,990 - Pf6N2E2_2117 0.29 -1.6 2,515,990 - Pf6N2E2_2117 0.29 -1.9 2,515,990 - Pf6N2E2_2117 0.29 -1.2 2,515,990 - Pf6N2E2_2117 0.29 -2.9 2,515,990 - Pf6N2E2_2117 0.29 +0.2 2,515,990 - Pf6N2E2_2117 0.29 -2.0 2,515,990 - Pf6N2E2_2117 0.29 -1.1 2,516,052 + Pf6N2E2_2117 0.31 -0.4 2,516,052 + Pf6N2E2_2117 0.31 -2.0 2,516,053 - Pf6N2E2_2117 0.31 -0.9 2,516,053 - Pf6N2E2_2117 0.31 -1.3 2,516,053 - Pf6N2E2_2117 0.31 -2.7 2,516,091 + Pf6N2E2_2117 0.33 +0.0 2,516,092 - Pf6N2E2_2117 0.33 -2.3 2,516,092 - Pf6N2E2_2117 0.33 -1.2 2,516,092 - Pf6N2E2_2117 0.33 -1.0 2,516,254 + Pf6N2E2_2117 0.40 -2.2 2,516,255 - Pf6N2E2_2117 0.41 -0.8 2,516,292 + Pf6N2E2_2117 0.42 -1.4 2,516,403 + Pf6N2E2_2117 0.47 -2.9 2,516,451 + Pf6N2E2_2117 0.49 -1.5 2,516,458 - Pf6N2E2_2117 0.50 -0.6 2,516,704 - Pf6N2E2_2117 0.61 -1.8 2,516,704 - Pf6N2E2_2117 0.61 -0.8 2,516,739 + Pf6N2E2_2117 0.62 -2.6 2,516,739 + Pf6N2E2_2117 0.62 -1.1 2,516,739 + Pf6N2E2_2117 0.62 +0.7 2,516,740 - Pf6N2E2_2117 0.62 -3.7 2,516,740 - Pf6N2E2_2117 0.62 -1.6 2,516,802 + Pf6N2E2_2117 0.65 +0.1 2,516,802 + Pf6N2E2_2117 0.65 -0.1 2,516,982 + -0.6 2,516,983 - Pf6N2E2_2117 0.73 -2.3 2,516,983 - Pf6N2E2_2117 0.73 -2.2 2,516,988 + Pf6N2E2_2117 0.73 +0.7 2,516,988 + Pf6N2E2_2117 0.73 -1.3 2,516,989 - Pf6N2E2_2117 0.73 -2.0 2,516,989 - Pf6N2E2_2117 0.73 -1.7 2,517,020 + Pf6N2E2_2117 0.75 -1.9 2,517,020 + Pf6N2E2_2117 0.75 -1.0 2,517,020 + Pf6N2E2_2117 0.75 -1.4 2,517,021 - Pf6N2E2_2117 0.75 -1.7 2,517,021 - Pf6N2E2_2117 0.75 -1.1 2,517,021 - Pf6N2E2_2117 0.75 +0.3 2,517,021 - Pf6N2E2_2117 0.75 -2.9 2,517,021 - Pf6N2E2_2117 0.75 -2.3 2,517,219 + Pf6N2E2_2117 0.84 -2.8 2,517,219 + Pf6N2E2_2117 0.84 -1.1 2,517,220 - Pf6N2E2_2117 0.84 -1.9 2,517,255 + Pf6N2E2_2117 0.85 -2.6 2,517,255 + Pf6N2E2_2117 0.85 -2.6 2,517,306 + Pf6N2E2_2117 0.88 -1.3 2,517,306 + Pf6N2E2_2117 0.88 -2.0 2,517,306 + Pf6N2E2_2117 0.88 -1.8 2,517,306 + Pf6N2E2_2117 0.88 -1.8 2,517,307 - Pf6N2E2_2117 0.88 -0.7 2,517,307 - Pf6N2E2_2117 0.88 -1.2 2,517,699 - -1.8 2,517,711 - -0.4 2,517,786 + +0.6 2,517,786 + +1.7 2,517,787 - -0.6 2,517,804 - -1.7 2,517,923 + -1.0 2,517,924 - +1.7 2,517,924 - -0.6 2,517,957 + Pf6N2E2_2118 0.11 +1.0 2,517,957 + Pf6N2E2_2118 0.11 +0.6 2,517,957 + Pf6N2E2_2118 0.11 +0.7 2,517,957 + Pf6N2E2_2118 0.11 -1.8 2,517,957 + Pf6N2E2_2118 0.11 +0.7 2,517,957 + Pf6N2E2_2118 0.11 +1.0 2,517,957 + Pf6N2E2_2118 0.11 -1.5 2,517,957 + Pf6N2E2_2118 0.11 -1.0 2,517,957 + Pf6N2E2_2118 0.11 +0.3 2,517,958 - Pf6N2E2_2118 0.11 +0.2 2,517,958 - Pf6N2E2_2118 0.11 -0.9 2,517,958 - Pf6N2E2_2118 0.11 -0.1 2,517,958 - Pf6N2E2_2118 0.11 -0.5 2,517,958 - Pf6N2E2_2118 0.11 -1.3 2,517,958 - Pf6N2E2_2118 0.11 -0.4 2,517,958 - Pf6N2E2_2118 0.11 -0.7 2,517,958 - Pf6N2E2_2118 0.11 -2.2 2,517,958 - Pf6N2E2_2118 0.11 +1.6 2,517,958 - Pf6N2E2_2118 0.11 -0.1 2,517,958 - Pf6N2E2_2118 0.11 +1.3 2,517,958 - Pf6N2E2_2118 0.11 +0.2 2,517,959 + Pf6N2E2_2118 0.11 -0.2 2,517,959 + Pf6N2E2_2118 0.11 +0.4 2,517,959 + Pf6N2E2_2118 0.11 -0.6 2,517,960 - Pf6N2E2_2118 0.11 +0.6 2,517,960 - Pf6N2E2_2118 0.11 +0.4 2,518,049 - Pf6N2E2_2118 0.14 -1.1 2,518,321 + Pf6N2E2_2118 0.22 -0.2 2,518,391 + Pf6N2E2_2118 0.24 +0.4 2,518,391 + Pf6N2E2_2118 0.24 -1.5 2,518,392 - Pf6N2E2_2118 0.24 -0.9 2,518,493 + Pf6N2E2_2118 0.27 -1.2 2,518,494 - Pf6N2E2_2118 0.27 +0.2
Or see this region's nucleotide sequence