Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1224 and Pf6N2E2_1225 are separated by 49 nucleotides Pf6N2E2_1225 and Pf6N2E2_1226 overlap by 4 nucleotides
Pf6N2E2_1224 - Two-component hybrid sensor and regulator, at 1,430,181 to 1,432,109
_1224
Pf6N2E2_1225 - DinG family ATP-dependent helicase CPE1197, at 1,432,159 to 1,434,471
_1225
Pf6N2E2_1226 - DNA damage response nuclease (from data) , at 1,434,468 to 1,436,138
_1226
Position (kb)
1432
1433
1434
1435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1431.529 kb on + strand, within Pf6N2E2_1224 at 1431.529 kb on + strand, within Pf6N2E2_1224 at 1431.529 kb on + strand, within Pf6N2E2_1224 at 1431.529 kb on + strand, within Pf6N2E2_1224 at 1431.530 kb on - strand, within Pf6N2E2_1224 at 1431.634 kb on + strand, within Pf6N2E2_1224 at 1431.634 kb on + strand, within Pf6N2E2_1224 at 1431.634 kb on + strand, within Pf6N2E2_1224 at 1431.635 kb on - strand, within Pf6N2E2_1224 at 1431.635 kb on - strand, within Pf6N2E2_1224 at 1431.635 kb on - strand, within Pf6N2E2_1224 at 1431.635 kb on - strand, within Pf6N2E2_1224 at 1431.635 kb on - strand, within Pf6N2E2_1224 at 1431.643 kb on + strand, within Pf6N2E2_1224 at 1431.916 kb on + strand, within Pf6N2E2_1224 at 1432.021 kb on + strand at 1432.021 kb on + strand at 1432.021 kb on + strand at 1432.022 kb on - strand at 1432.022 kb on - strand at 1432.022 kb on - strand at 1432.022 kb on - strand at 1432.022 kb on - strand at 1432.022 kb on - strand at 1432.031 kb on - strand at 1432.148 kb on + strand at 1432.148 kb on + strand at 1432.148 kb on + strand at 1432.149 kb on - strand at 1432.149 kb on - strand at 1432.149 kb on - strand at 1432.209 kb on + strand at 1432.347 kb on - strand at 1432.352 kb on + strand at 1432.352 kb on + strand at 1432.353 kb on - strand at 1432.353 kb on - strand at 1432.353 kb on - strand at 1432.353 kb on - strand at 1432.353 kb on - strand at 1432.353 kb on - strand at 1432.353 kb on - strand at 1432.358 kb on + strand at 1432.359 kb on - strand at 1432.645 kb on + strand, within Pf6N2E2_1225 at 1432.645 kb on + strand, within Pf6N2E2_1225 at 1432.646 kb on - strand, within Pf6N2E2_1225 at 1432.646 kb on - strand, within Pf6N2E2_1225 at 1432.649 kb on + strand, within Pf6N2E2_1225 at 1432.649 kb on + strand, within Pf6N2E2_1225 at 1432.649 kb on + strand, within Pf6N2E2_1225 at 1432.676 kb on + strand, within Pf6N2E2_1225 at 1432.677 kb on - strand, within Pf6N2E2_1225 at 1432.781 kb on - strand, within Pf6N2E2_1225 at 1432.859 kb on + strand, within Pf6N2E2_1225 at 1432.862 kb on + strand, within Pf6N2E2_1225 at 1432.862 kb on + strand, within Pf6N2E2_1225 at 1432.863 kb on - strand, within Pf6N2E2_1225 at 1432.863 kb on - strand, within Pf6N2E2_1225 at 1432.863 kb on - strand, within Pf6N2E2_1225 at 1432.965 kb on + strand, within Pf6N2E2_1225 at 1432.966 kb on - strand, within Pf6N2E2_1225 at 1433.066 kb on + strand, within Pf6N2E2_1225 at 1433.066 kb on + strand, within Pf6N2E2_1225 at 1433.067 kb on - strand, within Pf6N2E2_1225 at 1433.110 kb on + strand, within Pf6N2E2_1225 at 1433.111 kb on - strand, within Pf6N2E2_1225 at 1433.312 kb on + strand, within Pf6N2E2_1225 at 1433.312 kb on + strand, within Pf6N2E2_1225 at 1433.313 kb on - strand, within Pf6N2E2_1225 at 1433.420 kb on + strand, within Pf6N2E2_1225 at 1433.421 kb on - strand, within Pf6N2E2_1225 at 1433.423 kb on + strand, within Pf6N2E2_1225 at 1433.423 kb on + strand, within Pf6N2E2_1225 at 1433.423 kb on + strand, within Pf6N2E2_1225 at 1433.424 kb on - strand, within Pf6N2E2_1225 at 1433.424 kb on - strand, within Pf6N2E2_1225 at 1433.429 kb on + strand, within Pf6N2E2_1225 at 1433.429 kb on + strand, within Pf6N2E2_1225 at 1433.430 kb on - strand, within Pf6N2E2_1225 at 1433.443 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.481 kb on - strand, within Pf6N2E2_1225 at 1433.481 kb on - strand, within Pf6N2E2_1225 at 1433.579 kb on + strand, within Pf6N2E2_1225 at 1433.580 kb on - strand, within Pf6N2E2_1225 at 1433.701 kb on + strand, within Pf6N2E2_1225 at 1433.702 kb on - strand, within Pf6N2E2_1225 at 1433.702 kb on - strand, within Pf6N2E2_1225 at 1433.764 kb on - strand, within Pf6N2E2_1225 at 1433.998 kb on + strand, within Pf6N2E2_1225 at 1433.999 kb on - strand, within Pf6N2E2_1225 at 1434.054 kb on + strand, within Pf6N2E2_1225 at 1434.122 kb on - strand, within Pf6N2E2_1225 at 1434.122 kb on - strand, within Pf6N2E2_1225 at 1434.514 kb on + strand at 1434.514 kb on + strand at 1434.514 kb on + strand at 1434.515 kb on - strand at 1434.515 kb on - strand at 1434.774 kb on + strand, within Pf6N2E2_1226 at 1434.852 kb on + strand, within Pf6N2E2_1226 at 1434.853 kb on - strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.019 kb on - strand, within Pf6N2E2_1226 at 1435.019 kb on - strand, within Pf6N2E2_1226 at 1435.324 kb on + strand, within Pf6N2E2_1226 at 1435.324 kb on + strand, within Pf6N2E2_1226 at 1435.325 kb on - strand, within Pf6N2E2_1226
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 1,431,529 + Pf6N2E2_1224 0.70 -0.6 1,431,529 + Pf6N2E2_1224 0.70 -1.5 1,431,529 + Pf6N2E2_1224 0.70 +0.2 1,431,529 + Pf6N2E2_1224 0.70 -1.7 1,431,530 - Pf6N2E2_1224 0.70 -0.4 1,431,634 + Pf6N2E2_1224 0.75 +0.9 1,431,634 + Pf6N2E2_1224 0.75 +0.5 1,431,634 + Pf6N2E2_1224 0.75 +0.6 1,431,635 - Pf6N2E2_1224 0.75 +1.1 1,431,635 - Pf6N2E2_1224 0.75 -0.9 1,431,635 - Pf6N2E2_1224 0.75 -0.5 1,431,635 - Pf6N2E2_1224 0.75 +1.3 1,431,635 - Pf6N2E2_1224 0.75 +0.1 1,431,643 + Pf6N2E2_1224 0.76 +1.6 1,431,916 + Pf6N2E2_1224 0.90 +0.6 1,432,021 + +0.6 1,432,021 + -1.2 1,432,021 + -0.8 1,432,022 - -0.1 1,432,022 - -0.0 1,432,022 - +0.3 1,432,022 - +0.4 1,432,022 - -0.0 1,432,022 - -0.2 1,432,031 - -0.2 1,432,148 + +1.2 1,432,148 + -0.5 1,432,148 + +0.4 1,432,149 - +0.2 1,432,149 - +0.2 1,432,149 - -0.9 1,432,209 + +0.6 1,432,347 - +0.6 1,432,352 + -1.2 1,432,352 + -1.5 1,432,353 - +1.9 1,432,353 - -0.7 1,432,353 - -2.0 1,432,353 - -0.1 1,432,353 - -0.6 1,432,353 - -1.0 1,432,353 - -0.4 1,432,358 + +1.1 1,432,359 - -1.4 1,432,645 + Pf6N2E2_1225 0.21 -0.7 1,432,645 + Pf6N2E2_1225 0.21 +0.2 1,432,646 - Pf6N2E2_1225 0.21 -3.6 1,432,646 - Pf6N2E2_1225 0.21 -1.0 1,432,649 + Pf6N2E2_1225 0.21 -0.0 1,432,649 + Pf6N2E2_1225 0.21 -1.5 1,432,649 + Pf6N2E2_1225 0.21 +1.0 1,432,676 + Pf6N2E2_1225 0.22 +1.1 1,432,677 - Pf6N2E2_1225 0.22 -1.0 1,432,781 - Pf6N2E2_1225 0.27 +1.0 1,432,859 + Pf6N2E2_1225 0.30 +1.2 1,432,862 + Pf6N2E2_1225 0.30 -2.2 1,432,862 + Pf6N2E2_1225 0.30 +0.3 1,432,863 - Pf6N2E2_1225 0.30 +0.2 1,432,863 - Pf6N2E2_1225 0.30 +1.3 1,432,863 - Pf6N2E2_1225 0.30 +1.0 1,432,965 + Pf6N2E2_1225 0.35 +1.1 1,432,966 - Pf6N2E2_1225 0.35 +0.6 1,433,066 + Pf6N2E2_1225 0.39 +1.4 1,433,066 + Pf6N2E2_1225 0.39 -0.0 1,433,067 - Pf6N2E2_1225 0.39 +2.9 1,433,110 + Pf6N2E2_1225 0.41 +1.4 1,433,111 - Pf6N2E2_1225 0.41 -2.0 1,433,312 + Pf6N2E2_1225 0.50 +0.6 1,433,312 + Pf6N2E2_1225 0.50 +2.0 1,433,313 - Pf6N2E2_1225 0.50 +0.3 1,433,420 + Pf6N2E2_1225 0.55 -0.7 1,433,421 - Pf6N2E2_1225 0.55 -2.4 1,433,423 + Pf6N2E2_1225 0.55 +1.2 1,433,423 + Pf6N2E2_1225 0.55 +2.2 1,433,423 + Pf6N2E2_1225 0.55 +1.6 1,433,424 - Pf6N2E2_1225 0.55 -0.2 1,433,424 - Pf6N2E2_1225 0.55 +0.2 1,433,429 + Pf6N2E2_1225 0.55 -0.1 1,433,429 + Pf6N2E2_1225 0.55 -0.9 1,433,430 - Pf6N2E2_1225 0.55 -0.4 1,433,443 + Pf6N2E2_1225 0.56 -0.3 1,433,477 + Pf6N2E2_1225 0.57 -0.2 1,433,477 + Pf6N2E2_1225 0.57 +0.4 1,433,477 + Pf6N2E2_1225 0.57 -1.0 1,433,481 - Pf6N2E2_1225 0.57 +1.4 1,433,481 - Pf6N2E2_1225 0.57 +0.3 1,433,579 + Pf6N2E2_1225 0.61 -1.0 1,433,580 - Pf6N2E2_1225 0.61 +0.2 1,433,701 + Pf6N2E2_1225 0.67 +0.6 1,433,702 - Pf6N2E2_1225 0.67 +2.6 1,433,702 - Pf6N2E2_1225 0.67 +1.2 1,433,764 - Pf6N2E2_1225 0.69 +0.8 1,433,998 + Pf6N2E2_1225 0.80 -2.4 1,433,999 - Pf6N2E2_1225 0.80 +0.6 1,434,054 + Pf6N2E2_1225 0.82 -0.0 1,434,122 - Pf6N2E2_1225 0.85 +0.1 1,434,122 - Pf6N2E2_1225 0.85 +0.6 1,434,514 + +0.3 1,434,514 + -1.2 1,434,514 + +0.4 1,434,515 - -0.6 1,434,515 - +0.5 1,434,774 + Pf6N2E2_1226 0.18 +1.9 1,434,852 + Pf6N2E2_1226 0.23 +0.2 1,434,853 - Pf6N2E2_1226 0.23 -0.4 1,435,018 + Pf6N2E2_1226 0.33 +0.9 1,435,018 + Pf6N2E2_1226 0.33 +0.4 1,435,018 + Pf6N2E2_1226 0.33 +1.3 1,435,018 + Pf6N2E2_1226 0.33 -0.9 1,435,018 + Pf6N2E2_1226 0.33 -1.1 1,435,019 - Pf6N2E2_1226 0.33 -1.1 1,435,019 - Pf6N2E2_1226 0.33 +0.6 1,435,324 + Pf6N2E2_1226 0.51 -1.4 1,435,324 + Pf6N2E2_1226 0.51 -0.8 1,435,325 - Pf6N2E2_1226 0.51 -0.6
Or see this region's nucleotide sequence