Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_97 and Pf6N2E2_98 overlap by 4 nucleotides Pf6N2E2_98 and Pf6N2E2_99 are separated by 53 nucleotides Pf6N2E2_99 and Pf6N2E2_100 are separated by 298 nucleotides Pf6N2E2_100 and Pf6N2E2_101 are separated by 128 nucleotides Pf6N2E2_101 and Pf6N2E2_102 are separated by 338 nucleotides
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Pf6N2E2_98 - putative transmembrane protein, at 143,352 to 143,660
_98
Pf6N2E2_99 - hypothetical protein, at 143,714 to 143,854
_99
Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364
_100
Pf6N2E2_101 - CigR (Putative inner membrane protein), at 145,493 to 146,005
_101
Pf6N2E2_102 - sigma-54 dependent transcriptional regulator, at 146,344 to 148,188
_102
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 143.230 kb on - strand, within Pf6N2E2_97 at 143.392 kb on + strand, within Pf6N2E2_98 at 143.393 kb on - strand, within Pf6N2E2_98 at 143.629 kb on + strand, within Pf6N2E2_98 at 143.630 kb on - strand at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.768 kb on - strand, within Pf6N2E2_99 at 143.870 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.924 kb on - strand at 143.924 kb on - strand at 143.954 kb on - strand at 143.954 kb on - strand at 143.976 kb on - strand at 144.117 kb on + strand at 144.117 kb on + strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.128 kb on + strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.173 kb on - strand at 144.173 kb on - strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.276 kb on - strand, within Pf6N2E2_100 at 144.300 kb on + strand, within Pf6N2E2_100 at 144.403 kb on + strand, within Pf6N2E2_100 at 144.404 kb on - strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.600 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.631 kb on + strand, within Pf6N2E2_100 at 144.827 kb on - strand, within Pf6N2E2_100 at 144.900 kb on + strand, within Pf6N2E2_100 at 144.901 kb on - strand, within Pf6N2E2_100 at 144.951 kb on + strand, within Pf6N2E2_100 at 144.951 kb on + strand, within Pf6N2E2_100 at 144.961 kb on + strand, within Pf6N2E2_100 at 144.979 kb on + strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.985 kb on + strand, within Pf6N2E2_100 at 144.985 kb on + strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 145.000 kb on + strand, within Pf6N2E2_100 at 145.001 kb on - strand, within Pf6N2E2_100 at 145.079 kb on - strand, within Pf6N2E2_100 at 145.079 kb on - strand, within Pf6N2E2_100 at 145.136 kb on - strand, within Pf6N2E2_100 at 145.352 kb on - strand at 145.352 kb on - strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.393 kb on + strand at 145.393 kb on + strand at 145.433 kb on + strand at 145.434 kb on - strand at 145.434 kb on - strand at 145.436 kb on + strand at 145.437 kb on - strand at 145.437 kb on - strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.455 kb on - strand at 145.455 kb on - strand at 145.455 kb on - strand at 145.460 kb on + strand at 145.630 kb on + strand, within Pf6N2E2_101 at 145.630 kb on + strand, within Pf6N2E2_101 at 145.630 kb on + strand, within Pf6N2E2_101 at 145.631 kb on - strand, within Pf6N2E2_101 at 145.907 kb on + strand, within Pf6N2E2_101 at 145.908 kb on - strand, within Pf6N2E2_101 at 145.917 kb on - strand, within Pf6N2E2_101 at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 146.004 kb on - strand at 146.014 kb on - strand at 146.027 kb on + strand at 146.027 kb on + strand at 146.028 kb on - strand at 146.143 kb on - strand at 146.143 kb on - strand at 146.143 kb on - strand at 146.268 kb on + strand at 146.268 kb on + strand at 146.268 kb on + strand at 146.294 kb on + strand at 146.294 kb on + strand at 146.294 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 143,230 - Pf6N2E2_97 0.82 -0.7 143,392 + Pf6N2E2_98 0.13 +0.9 143,393 - Pf6N2E2_98 0.13 +1.0 143,629 + Pf6N2E2_98 0.90 -1.2 143,630 - -0.9 143,757 - Pf6N2E2_99 0.30 -0.1 143,757 - Pf6N2E2_99 0.30 +0.1 143,757 - Pf6N2E2_99 0.30 -0.8 143,768 - Pf6N2E2_99 0.38 +1.1 143,870 + -0.8 143,923 + +1.1 143,923 + -0.3 143,923 + -0.3 143,923 + -0.9 143,923 + +1.3 143,923 + -0.2 143,924 - +0.1 143,924 - +0.5 143,954 - -0.4 143,954 - -0.5 143,976 - +0.8 144,117 + +0.4 144,117 + +0.3 144,118 - -0.1 144,118 - -0.5 144,118 - +1.5 144,118 - +0.9 144,128 + -0.4 144,129 - +0.4 144,129 - +0.1 144,129 - +1.1 144,129 - +0.7 144,132 - +0.9 144,132 - -0.3 144,132 - -1.3 144,132 - -0.5 144,132 - -0.2 144,172 + +0.2 144,172 + +1.5 144,172 + -0.9 144,173 - -0.3 144,173 - +0.4 144,196 + -0.6 144,196 + -0.1 144,196 + -1.2 144,197 - -0.3 144,197 - -0.6 144,197 - +0.7 144,276 - Pf6N2E2_100 0.10 -1.0 144,300 + Pf6N2E2_100 0.12 -0.2 144,403 + Pf6N2E2_100 0.21 +0.1 144,404 - Pf6N2E2_100 0.21 -2.3 144,511 + Pf6N2E2_100 0.30 +0.7 144,511 + Pf6N2E2_100 0.30 -1.2 144,512 - Pf6N2E2_100 0.30 -1.2 144,512 - Pf6N2E2_100 0.30 +0.1 144,600 + Pf6N2E2_100 0.37 +0.1 144,610 + Pf6N2E2_100 0.38 +0.0 144,610 + Pf6N2E2_100 0.38 -0.5 144,610 + Pf6N2E2_100 0.38 -0.9 144,631 + Pf6N2E2_100 0.39 +1.9 144,827 - Pf6N2E2_100 0.56 -0.6 144,900 + Pf6N2E2_100 0.62 -0.2 144,901 - Pf6N2E2_100 0.62 +0.1 144,951 + Pf6N2E2_100 0.66 -1.6 144,951 + Pf6N2E2_100 0.66 +1.0 144,961 + Pf6N2E2_100 0.67 -1.3 144,979 + Pf6N2E2_100 0.68 -0.8 144,980 - Pf6N2E2_100 0.68 -0.2 144,980 - Pf6N2E2_100 0.68 +0.0 144,980 - Pf6N2E2_100 0.68 +0.2 144,985 + Pf6N2E2_100 0.69 +0.6 144,985 + Pf6N2E2_100 0.69 -1.3 144,986 - Pf6N2E2_100 0.69 -0.5 144,986 - Pf6N2E2_100 0.69 -1.2 144,986 - Pf6N2E2_100 0.69 -0.0 144,986 - Pf6N2E2_100 0.69 -0.1 144,986 - Pf6N2E2_100 0.69 +0.0 145,000 + Pf6N2E2_100 0.70 -1.9 145,001 - Pf6N2E2_100 0.70 -0.1 145,079 - Pf6N2E2_100 0.76 -0.4 145,079 - Pf6N2E2_100 0.76 -0.5 145,136 - Pf6N2E2_100 0.81 -0.4 145,352 - -1.1 145,352 - +0.8 145,391 + -0.1 145,391 + +1.9 145,391 + -0.1 145,391 + -1.0 145,391 + -0.6 145,392 - -0.3 145,392 - +0.2 145,392 - +1.1 145,392 - -0.7 145,393 + +0.4 145,393 + +0.6 145,433 + +0.2 145,434 - -0.7 145,434 - -0.0 145,436 + +0.3 145,437 - -1.7 145,437 - -1.4 145,452 + +0.1 145,452 + -1.9 145,452 + +0.1 145,452 + -0.7 145,452 + -0.2 145,452 + -0.1 145,453 - -0.3 145,453 - -0.9 145,453 - +1.2 145,453 - -0.8 145,454 + -0.5 145,454 + -0.5 145,454 + -0.2 145,454 + +1.3 145,455 - +0.3 145,455 - -1.7 145,455 - -0.1 145,460 + +0.2 145,630 + Pf6N2E2_101 0.27 +0.4 145,630 + Pf6N2E2_101 0.27 -1.1 145,630 + Pf6N2E2_101 0.27 +0.5 145,631 - Pf6N2E2_101 0.27 -0.8 145,907 + Pf6N2E2_101 0.81 +0.5 145,908 - Pf6N2E2_101 0.81 -0.9 145,917 - Pf6N2E2_101 0.83 -0.5 145,976 + -2.5 145,976 + -0.2 145,976 + -0.3 145,976 + +0.4 145,976 + +0.3 145,977 - +0.3 145,977 - +1.7 145,977 - +0.3 145,977 - +1.6 145,977 - +1.1 145,977 - +0.1 146,004 - -0.0 146,014 - -1.4 146,027 + -0.0 146,027 + +0.4 146,028 - -0.0 146,143 - -0.0 146,143 - +0.4 146,143 - +1.7 146,268 + -0.6 146,268 + +0.6 146,268 + -0.9 146,294 + -0.4 146,294 + -0.4 146,294 + +0.1 146,298 + -1.5 146,298 + +0.6 146,298 + +1.7 146,298 + +0.8 146,298 + +0.1 146,298 + +0.3 146,342 + -0.3
Or see this region's nucleotide sequence