Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_396 and Pf6N2E2_397 are separated by 162 nucleotides Pf6N2E2_397 and Pf6N2E2_398 are separated by 209 nucleotides Pf6N2E2_398 and Pf6N2E2_399 overlap by 4 nucleotides
Pf6N2E2_396 - hypothetical protein, at 464,513 to 464,920
_396
Pf6N2E2_397 - Large exoproteins involved in heme utilization or adhesion, at 465,083 to 467,020
_397
Pf6N2E2_398 - FIG006045: Sigma factor, ECF subfamily, at 467,230 to 467,736
_398
Pf6N2E2_399 - Iron siderophore sensor protein, at 467,733 to 468,656
_399
Position (kb) 465
466
467
468 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 464.475 kb on + strand at 464.476 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.512 kb on - strand at 464.512 kb on - strand at 464.532 kb on + strand at 464.532 kb on + strand at 464.533 kb on - strand at 464.533 kb on - strand at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.690 kb on + strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.846 kb on + strand, within Pf6N2E2_396 at 464.857 kb on + strand, within Pf6N2E2_396 at 464.900 kb on + strand at 464.900 kb on + strand at 464.901 kb on - strand at 464.901 kb on - strand at 464.901 kb on - strand at 464.913 kb on + strand at 464.913 kb on + strand at 464.914 kb on - strand at 464.914 kb on - strand at 464.914 kb on - strand at 464.918 kb on + strand at 464.918 kb on + strand at 464.918 kb on + strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.930 kb on + strand at 465.034 kb on + strand at 465.035 kb on - strand at 465.084 kb on + strand at 465.084 kb on + strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.225 kb on + strand at 465.225 kb on + strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.348 kb on + strand, within Pf6N2E2_397 at 465.391 kb on - strand, within Pf6N2E2_397 at 465.404 kb on + strand, within Pf6N2E2_397 at 465.404 kb on + strand, within Pf6N2E2_397 at 465.446 kb on + strand, within Pf6N2E2_397 at 465.446 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.551 kb on + strand, within Pf6N2E2_397 at 465.552 kb on - strand, within Pf6N2E2_397 at 465.691 kb on - strand, within Pf6N2E2_397 at 465.820 kb on + strand, within Pf6N2E2_397 at 465.843 kb on + strand, within Pf6N2E2_397 at 465.874 kb on + strand, within Pf6N2E2_397 at 465.875 kb on - strand, within Pf6N2E2_397 at 466.014 kb on + strand, within Pf6N2E2_397 at 466.014 kb on + strand, within Pf6N2E2_397 at 466.014 kb on + strand, within Pf6N2E2_397 at 466.015 kb on - strand, within Pf6N2E2_397 at 466.015 kb on - strand, within Pf6N2E2_397 at 466.017 kb on + strand, within Pf6N2E2_397 at 466.018 kb on - strand, within Pf6N2E2_397 at 466.089 kb on + strand, within Pf6N2E2_397 at 466.146 kb on - strand, within Pf6N2E2_397 at 466.418 kb on + strand, within Pf6N2E2_397 at 466.419 kb on - strand, within Pf6N2E2_397 at 466.419 kb on - strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.551 kb on + strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.552 kb on - strand, within Pf6N2E2_397 at 466.628 kb on + strand, within Pf6N2E2_397 at 466.629 kb on - strand, within Pf6N2E2_397 at 466.644 kb on + strand, within Pf6N2E2_397 at 466.708 kb on + strand, within Pf6N2E2_397 at 466.708 kb on + strand, within Pf6N2E2_397 at 466.749 kb on + strand, within Pf6N2E2_397 at 466.749 kb on + strand, within Pf6N2E2_397 at 466.749 kb on + strand, within Pf6N2E2_397 at 466.750 kb on - strand, within Pf6N2E2_397 at 466.750 kb on - strand, within Pf6N2E2_397 at 466.834 kb on + strand at 466.835 kb on - strand at 466.936 kb on + strand at 466.937 kb on - strand at 466.938 kb on + strand at 466.939 kb on - strand at 466.939 kb on - strand at 466.939 kb on - strand at 466.954 kb on + strand at 466.955 kb on - strand at 467.084 kb on - strand at 467.087 kb on + strand at 467.088 kb on - strand at 467.088 kb on - strand at 467.088 kb on - strand at 467.088 kb on - strand at 467.088 kb on - strand at 467.088 kb on - strand at 467.088 kb on - strand at 467.108 kb on - strand at 467.486 kb on + strand, within Pf6N2E2_398 at 467.487 kb on - strand, within Pf6N2E2_398 at 467.487 kb on - strand, within Pf6N2E2_398 at 467.487 kb on - strand, within Pf6N2E2_398 at 467.683 kb on + strand, within Pf6N2E2_398 at 467.683 kb on + strand, within Pf6N2E2_398 at 467.683 kb on + strand, within Pf6N2E2_398 at 467.683 kb on + strand, within Pf6N2E2_398 at 467.683 kb on + strand, within Pf6N2E2_398 at 467.683 kb on + strand, within Pf6N2E2_398 at 467.684 kb on - strand, within Pf6N2E2_398 at 467.684 kb on - strand, within Pf6N2E2_398 at 467.684 kb on - strand, within Pf6N2E2_398 at 467.684 kb on - strand, within Pf6N2E2_398 at 467.685 kb on + strand, within Pf6N2E2_398 at 467.902 kb on + strand, within Pf6N2E2_399 at 467.903 kb on - strand, within Pf6N2E2_399 at 467.903 kb on - strand, within Pf6N2E2_399
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 464,475 + -2.0 464,476 - +0.9 464,508 - -0.5 464,508 - +3.5 464,508 - +0.7 464,508 - -1.2 464,511 + -0.6 464,511 + -2.4 464,511 + +1.2 464,511 + +1.2 464,512 - +1.0 464,512 - -1.4 464,532 + +0.8 464,532 + +0.3 464,533 - +1.1 464,533 - +2.0 464,594 - Pf6N2E2_396 0.20 +0.8 464,594 - Pf6N2E2_396 0.20 +0.4 464,594 - Pf6N2E2_396 0.20 +0.3 464,690 + Pf6N2E2_396 0.43 +0.5 464,719 - Pf6N2E2_396 0.50 +0.1 464,719 - Pf6N2E2_396 0.50 -0.4 464,812 + Pf6N2E2_396 0.73 -0.9 464,812 + Pf6N2E2_396 0.73 +1.0 464,812 + Pf6N2E2_396 0.73 +0.6 464,846 + Pf6N2E2_396 0.82 -0.3 464,857 + Pf6N2E2_396 0.84 -0.0 464,900 + +1.5 464,900 + +0.5 464,901 - +1.0 464,901 - +0.2 464,901 - +0.1 464,913 + -0.7 464,913 + -1.5 464,914 - +1.8 464,914 - +0.6 464,914 - -0.0 464,918 + -0.6 464,918 + +0.2 464,918 + -0.0 464,919 - -2.0 464,919 - +0.3 464,919 - +0.3 464,919 - -0.6 464,919 - -0.0 464,919 - -0.0 464,919 - +0.8 464,930 + +0.6 465,034 + -0.3 465,035 - +1.2 465,084 + +0.8 465,084 + +0.2 465,085 - +0.3 465,085 - -0.8 465,085 - +0.5 465,085 - -0.4 465,085 - -0.2 465,225 + -1.8 465,225 + +2.0 465,226 - +1.2 465,226 - -0.0 465,226 - -0.2 465,226 - +0.3 465,226 - -0.8 465,226 - -0.4 465,226 - -1.1 465,348 + Pf6N2E2_397 0.14 +0.5 465,391 - Pf6N2E2_397 0.16 +0.1 465,404 + Pf6N2E2_397 0.17 -0.3 465,404 + Pf6N2E2_397 0.17 +0.1 465,446 + Pf6N2E2_397 0.19 -0.4 465,446 + Pf6N2E2_397 0.19 +0.1 465,473 + Pf6N2E2_397 0.20 -1.4 465,473 + Pf6N2E2_397 0.20 -0.3 465,473 + Pf6N2E2_397 0.20 +0.1 465,473 + Pf6N2E2_397 0.20 +1.4 465,474 - Pf6N2E2_397 0.20 +0.1 465,474 - Pf6N2E2_397 0.20 +1.0 465,474 - Pf6N2E2_397 0.20 +0.7 465,474 - Pf6N2E2_397 0.20 +0.8 465,474 - Pf6N2E2_397 0.20 +0.0 465,474 - Pf6N2E2_397 0.20 +0.0 465,474 - Pf6N2E2_397 0.20 +0.2 465,551 + Pf6N2E2_397 0.24 -1.2 465,552 - Pf6N2E2_397 0.24 +0.7 465,691 - Pf6N2E2_397 0.31 -0.3 465,820 + Pf6N2E2_397 0.38 -0.3 465,843 + Pf6N2E2_397 0.39 -0.5 465,874 + Pf6N2E2_397 0.41 +0.8 465,875 - Pf6N2E2_397 0.41 -0.4 466,014 + Pf6N2E2_397 0.48 +1.4 466,014 + Pf6N2E2_397 0.48 -0.8 466,014 + Pf6N2E2_397 0.48 -0.2 466,015 - Pf6N2E2_397 0.48 -0.2 466,015 - Pf6N2E2_397 0.48 +1.7 466,017 + Pf6N2E2_397 0.48 -0.2 466,018 - Pf6N2E2_397 0.48 +1.4 466,089 + Pf6N2E2_397 0.52 +0.6 466,146 - Pf6N2E2_397 0.55 -0.4 466,418 + Pf6N2E2_397 0.69 -0.4 466,419 - Pf6N2E2_397 0.69 -1.2 466,419 - Pf6N2E2_397 0.69 -0.8 466,551 + Pf6N2E2_397 0.76 +1.1 466,551 + Pf6N2E2_397 0.76 -0.0 466,551 + Pf6N2E2_397 0.76 +0.1 466,551 + Pf6N2E2_397 0.76 +1.3 466,551 + Pf6N2E2_397 0.76 -0.5 466,551 + Pf6N2E2_397 0.76 -0.5 466,551 + Pf6N2E2_397 0.76 +0.3 466,551 + Pf6N2E2_397 0.76 -0.8 466,551 + Pf6N2E2_397 0.76 -0.5 466,551 + Pf6N2E2_397 0.76 +1.8 466,551 + Pf6N2E2_397 0.76 +1.1 466,551 + Pf6N2E2_397 0.76 -0.6 466,551 + Pf6N2E2_397 0.76 -0.0 466,551 + Pf6N2E2_397 0.76 +0.3 466,551 + Pf6N2E2_397 0.76 -1.0 466,552 - Pf6N2E2_397 0.76 -0.7 466,552 - Pf6N2E2_397 0.76 -0.4 466,552 - Pf6N2E2_397 0.76 +1.4 466,552 - Pf6N2E2_397 0.76 -0.9 466,552 - Pf6N2E2_397 0.76 -0.9 466,552 - Pf6N2E2_397 0.76 +0.1 466,552 - Pf6N2E2_397 0.76 -1.0 466,552 - Pf6N2E2_397 0.76 +0.1 466,552 - Pf6N2E2_397 0.76 +0.2 466,552 - +1.0 466,552 - Pf6N2E2_397 0.76 +0.6 466,552 - Pf6N2E2_397 0.76 +0.4 466,552 - Pf6N2E2_397 0.76 -0.3 466,552 - Pf6N2E2_397 0.76 +0.3 466,552 - Pf6N2E2_397 0.76 -0.0 466,552 - Pf6N2E2_397 0.76 -0.2 466,552 - Pf6N2E2_397 0.76 -0.7 466,552 - Pf6N2E2_397 0.76 -1.9 466,552 - Pf6N2E2_397 0.76 +0.7 466,552 - Pf6N2E2_397 0.76 +1.1 466,628 + Pf6N2E2_397 0.80 -0.2 466,629 - Pf6N2E2_397 0.80 +0.2 466,644 + Pf6N2E2_397 0.81 -1.0 466,708 + Pf6N2E2_397 0.84 +0.4 466,708 + Pf6N2E2_397 0.84 +2.2 466,749 + Pf6N2E2_397 0.86 +1.4 466,749 + Pf6N2E2_397 0.86 +0.2 466,749 + Pf6N2E2_397 0.86 +0.3 466,750 - Pf6N2E2_397 0.86 -0.4 466,750 - Pf6N2E2_397 0.86 -0.0 466,834 + -1.0 466,835 - +0.3 466,936 + -1.2 466,937 - +0.3 466,938 + +0.2 466,939 - -0.1 466,939 - -0.8 466,939 - -0.6 466,954 + -0.0 466,955 - -0.3 467,084 - +0.8 467,087 + -0.2 467,088 - -0.3 467,088 - +0.3 467,088 - +0.5 467,088 - -0.9 467,088 - +1.2 467,088 - +1.0 467,088 - +1.7 467,108 - +0.6 467,486 + Pf6N2E2_398 0.50 +1.4 467,487 - Pf6N2E2_398 0.51 -0.2 467,487 - Pf6N2E2_398 0.51 +0.2 467,487 - Pf6N2E2_398 0.51 +0.4 467,683 + Pf6N2E2_398 0.89 -0.0 467,683 + Pf6N2E2_398 0.89 -0.5 467,683 + Pf6N2E2_398 0.89 -0.9 467,683 + Pf6N2E2_398 0.89 -0.9 467,683 + Pf6N2E2_398 0.89 -0.0 467,683 + Pf6N2E2_398 0.89 -0.9 467,684 - Pf6N2E2_398 0.90 -0.8 467,684 - Pf6N2E2_398 0.90 -0.5 467,684 - Pf6N2E2_398 0.90 +0.3 467,684 - Pf6N2E2_398 0.90 -1.3 467,685 + Pf6N2E2_398 0.90 -2.0 467,902 + Pf6N2E2_399 0.18 +0.4 467,903 - Pf6N2E2_399 0.18 -0.4 467,903 - Pf6N2E2_399 0.18 +0.1
Or see this region's nucleotide sequence