Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2935 and Pf6N2E2_2936 are separated by 148 nucleotides Pf6N2E2_2936 and Pf6N2E2_2937 are separated by 266 nucleotides
Pf6N2E2_2935 - NAD(FAD)-utilizing dehydrogenase, sll0175 homolog, at 3,425,682 to 3,427,295
_2935
Pf6N2E2_2936 - Cysteine synthase (EC 2.5.1.47), at 3,427,444 to 3,428,538
_2936
Pf6N2E2_2937 - Na+/H+ antiporter NhaA type, at 3,428,805 to 3,429,992
_2937
Position (kb)
3427
3428
3429 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3426.519 kb on + strand, within Pf6N2E2_2935 at 3426.519 kb on + strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.520 kb on - strand, within Pf6N2E2_2935 at 3426.600 kb on + strand, within Pf6N2E2_2935 at 3426.600 kb on + strand, within Pf6N2E2_2935 at 3426.633 kb on + strand, within Pf6N2E2_2935 at 3426.672 kb on + strand, within Pf6N2E2_2935 at 3426.820 kb on - strand, within Pf6N2E2_2935 at 3426.893 kb on + strand, within Pf6N2E2_2935 at 3426.893 kb on + strand, within Pf6N2E2_2935 at 3426.893 kb on + strand, within Pf6N2E2_2935 at 3426.893 kb on + strand, within Pf6N2E2_2935 at 3426.893 kb on + strand, within Pf6N2E2_2935 at 3426.897 kb on + strand, within Pf6N2E2_2935 at 3426.897 kb on + strand, within Pf6N2E2_2935 at 3426.897 kb on + strand, within Pf6N2E2_2935 at 3426.897 kb on + strand, within Pf6N2E2_2935 at 3426.898 kb on - strand, within Pf6N2E2_2935 at 3426.898 kb on - strand, within Pf6N2E2_2935 at 3426.975 kb on + strand, within Pf6N2E2_2935 at 3427.209 kb on + strand at 3427.314 kb on + strand at 3427.314 kb on + strand at 3427.314 kb on + strand at 3427.314 kb on + strand at 3427.314 kb on + strand at 3427.315 kb on - strand at 3427.315 kb on - strand at 3427.315 kb on - strand at 3427.315 kb on - strand at 3427.315 kb on - strand at 3427.318 kb on + strand at 3427.318 kb on + strand at 3427.318 kb on + strand at 3427.318 kb on + strand at 3427.319 kb on - strand at 3427.319 kb on - strand at 3427.377 kb on + strand at 3427.532 kb on + strand at 3427.562 kb on + strand, within Pf6N2E2_2936 at 3427.563 kb on - strand, within Pf6N2E2_2936 at 3427.565 kb on + strand, within Pf6N2E2_2936 at 3427.565 kb on + strand, within Pf6N2E2_2936 at 3427.566 kb on - strand, within Pf6N2E2_2936 at 3427.718 kb on + strand, within Pf6N2E2_2936 at 3427.719 kb on - strand, within Pf6N2E2_2936 at 3427.719 kb on - strand at 3427.865 kb on + strand, within Pf6N2E2_2936 at 3427.865 kb on + strand, within Pf6N2E2_2936 at 3427.866 kb on - strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.937 kb on + strand, within Pf6N2E2_2936 at 3427.938 kb on - strand, within Pf6N2E2_2936 at 3427.938 kb on - strand, within Pf6N2E2_2936 at 3427.938 kb on - strand, within Pf6N2E2_2936 at 3427.938 kb on - strand, within Pf6N2E2_2936 at 3427.938 kb on - strand, within Pf6N2E2_2936 at 3427.938 kb on - strand, within Pf6N2E2_2936 at 3427.959 kb on + strand, within Pf6N2E2_2936 at 3428.081 kb on + strand, within Pf6N2E2_2936 at 3428.142 kb on - strand, within Pf6N2E2_2936 at 3428.142 kb on - strand, within Pf6N2E2_2936 at 3428.142 kb on - strand, within Pf6N2E2_2936 at 3428.152 kb on - strand, within Pf6N2E2_2936 at 3428.183 kb on + strand, within Pf6N2E2_2936 at 3428.183 kb on + strand, within Pf6N2E2_2936 at 3428.184 kb on - strand, within Pf6N2E2_2936 at 3428.267 kb on + strand, within Pf6N2E2_2936 at 3428.267 kb on + strand, within Pf6N2E2_2936 at 3428.268 kb on - strand, within Pf6N2E2_2936 at 3428.268 kb on - strand, within Pf6N2E2_2936 at 3428.305 kb on + strand, within Pf6N2E2_2936 at 3428.348 kb on + strand, within Pf6N2E2_2936 at 3428.417 kb on + strand, within Pf6N2E2_2936 at 3428.418 kb on - strand, within Pf6N2E2_2936 at 3428.466 kb on + strand at 3428.466 kb on + strand at 3428.466 kb on + strand at 3428.467 kb on - strand at 3428.627 kb on - strand at 3428.659 kb on + strand at 3428.659 kb on + strand at 3428.659 kb on + strand at 3428.659 kb on + strand at 3428.659 kb on + strand at 3428.660 kb on - strand at 3428.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,426,519 + Pf6N2E2_2935 0.52 +0.9 3,426,519 + Pf6N2E2_2935 0.52 +1.6 3,426,520 - Pf6N2E2_2935 0.52 +0.9 3,426,520 - Pf6N2E2_2935 0.52 -1.4 3,426,520 - Pf6N2E2_2935 0.52 -0.1 3,426,520 - Pf6N2E2_2935 0.52 +0.6 3,426,520 - Pf6N2E2_2935 0.52 +0.8 3,426,520 - Pf6N2E2_2935 0.52 +2.0 3,426,520 - Pf6N2E2_2935 0.52 -0.9 3,426,520 - Pf6N2E2_2935 0.52 -1.0 3,426,600 + Pf6N2E2_2935 0.57 +0.5 3,426,600 + Pf6N2E2_2935 0.57 -0.2 3,426,633 + Pf6N2E2_2935 0.59 -0.9 3,426,672 + Pf6N2E2_2935 0.61 +0.7 3,426,820 - Pf6N2E2_2935 0.71 -0.5 3,426,893 + Pf6N2E2_2935 0.75 +0.8 3,426,893 + Pf6N2E2_2935 0.75 -1.5 3,426,893 + Pf6N2E2_2935 0.75 +0.2 3,426,893 + Pf6N2E2_2935 0.75 +0.1 3,426,893 + Pf6N2E2_2935 0.75 -0.2 3,426,897 + Pf6N2E2_2935 0.75 -0.6 3,426,897 + Pf6N2E2_2935 0.75 +0.1 3,426,897 + Pf6N2E2_2935 0.75 -1.1 3,426,897 + Pf6N2E2_2935 0.75 +0.7 3,426,898 - Pf6N2E2_2935 0.75 +0.1 3,426,898 - Pf6N2E2_2935 0.75 -0.4 3,426,975 + Pf6N2E2_2935 0.80 -0.9 3,427,209 + -0.2 3,427,314 + +0.4 3,427,314 + +0.7 3,427,314 + -1.8 3,427,314 + +0.7 3,427,314 + -0.4 3,427,315 - +0.0 3,427,315 - +0.4 3,427,315 - -0.9 3,427,315 - -0.0 3,427,315 - -1.2 3,427,318 + -0.5 3,427,318 + +0.0 3,427,318 + -1.3 3,427,318 + +0.5 3,427,319 - +1.8 3,427,319 - -0.6 3,427,377 + +0.6 3,427,532 + +0.2 3,427,562 + Pf6N2E2_2936 0.11 +1.1 3,427,563 - Pf6N2E2_2936 0.11 -0.7 3,427,565 + Pf6N2E2_2936 0.11 -1.2 3,427,565 + Pf6N2E2_2936 0.11 -0.4 3,427,566 - Pf6N2E2_2936 0.11 +1.2 3,427,718 + Pf6N2E2_2936 0.25 -0.4 3,427,719 - Pf6N2E2_2936 0.25 -0.6 3,427,719 - -0.9 3,427,865 + Pf6N2E2_2936 0.38 -0.4 3,427,865 + Pf6N2E2_2936 0.38 -0.7 3,427,866 - Pf6N2E2_2936 0.39 +0.8 3,427,937 + Pf6N2E2_2936 0.45 +0.2 3,427,937 + Pf6N2E2_2936 0.45 +0.2 3,427,937 + Pf6N2E2_2936 0.45 +0.4 3,427,937 + Pf6N2E2_2936 0.45 +1.0 3,427,937 + Pf6N2E2_2936 0.45 +1.2 3,427,937 + Pf6N2E2_2936 0.45 -0.3 3,427,937 + Pf6N2E2_2936 0.45 -0.6 3,427,938 - Pf6N2E2_2936 0.45 +0.2 3,427,938 - Pf6N2E2_2936 0.45 +0.5 3,427,938 - Pf6N2E2_2936 0.45 -0.1 3,427,938 - Pf6N2E2_2936 0.45 -0.4 3,427,938 - Pf6N2E2_2936 0.45 -1.2 3,427,938 - Pf6N2E2_2936 0.45 +0.3 3,427,959 + Pf6N2E2_2936 0.47 +0.4 3,428,081 + Pf6N2E2_2936 0.58 +0.7 3,428,142 - Pf6N2E2_2936 0.64 -0.8 3,428,142 - Pf6N2E2_2936 0.64 +0.7 3,428,142 - Pf6N2E2_2936 0.64 -0.5 3,428,152 - Pf6N2E2_2936 0.65 +1.4 3,428,183 + Pf6N2E2_2936 0.67 +1.0 3,428,183 + Pf6N2E2_2936 0.67 -0.8 3,428,184 - Pf6N2E2_2936 0.68 +0.8 3,428,267 + Pf6N2E2_2936 0.75 -0.7 3,428,267 + Pf6N2E2_2936 0.75 -0.7 3,428,268 - Pf6N2E2_2936 0.75 +2.8 3,428,268 - Pf6N2E2_2936 0.75 +0.5 3,428,305 + Pf6N2E2_2936 0.79 -0.3 3,428,348 + Pf6N2E2_2936 0.83 +1.1 3,428,417 + Pf6N2E2_2936 0.89 +0.2 3,428,418 - Pf6N2E2_2936 0.89 -0.2 3,428,466 + +0.3 3,428,466 + -0.5 3,428,466 + +1.4 3,428,467 - +1.9 3,428,627 - -1.4 3,428,659 + +0.1 3,428,659 + +1.0 3,428,659 + -0.1 3,428,659 + -0.2 3,428,659 + +1.1 3,428,660 - -0.5 3,428,669 + -0.7
Or see this region's nucleotide sequence