Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1767 and Pf6N2E2_1768 are separated by 57 nucleotides Pf6N2E2_1768 and Pf6N2E2_1769 overlap by 4 nucleotides Pf6N2E2_1769 and Pf6N2E2_1770 are separated by 44 nucleotides
Pf6N2E2_1767 - Transcriptional regulator, AraC family, at 2,088,739 to 2,089,500
_1767
Pf6N2E2_1768 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,089,558 to 2,090,982
_1768
Pf6N2E2_1769 - Nucleoside-diphosphate-sugar epimerases, at 2,090,979 to 2,091,320
_1769
Pf6N2E2_1770 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 2,091,365 to 2,092,438
_1770
Position (kb)
2089
2090
2091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2088.582 kb on + strand at 2088.583 kb on - strand at 2088.583 kb on - strand at 2088.583 kb on - strand at 2088.601 kb on + strand at 2088.634 kb on + strand at 2088.812 kb on - strand at 2088.812 kb on - strand at 2088.880 kb on + strand, within Pf6N2E2_1767 at 2088.881 kb on - strand, within Pf6N2E2_1767 at 2088.916 kb on + strand, within Pf6N2E2_1767 at 2088.917 kb on - strand, within Pf6N2E2_1767 at 2088.917 kb on - strand, within Pf6N2E2_1767 at 2089.057 kb on - strand, within Pf6N2E2_1767 at 2089.057 kb on - strand, within Pf6N2E2_1767 at 2089.058 kb on + strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.125 kb on + strand, within Pf6N2E2_1767 at 2089.125 kb on + strand, within Pf6N2E2_1767 at 2089.126 kb on - strand, within Pf6N2E2_1767 at 2089.180 kb on + strand, within Pf6N2E2_1767 at 2089.249 kb on + strand, within Pf6N2E2_1767 at 2089.270 kb on + strand, within Pf6N2E2_1767 at 2089.270 kb on + strand, within Pf6N2E2_1767 at 2089.270 kb on + strand, within Pf6N2E2_1767 at 2089.303 kb on + strand, within Pf6N2E2_1767 at 2089.304 kb on - strand, within Pf6N2E2_1767 at 2089.304 kb on - strand, within Pf6N2E2_1767 at 2089.309 kb on + strand, within Pf6N2E2_1767 at 2089.423 kb on + strand, within Pf6N2E2_1767 at 2089.499 kb on - strand at 2089.630 kb on + strand at 2089.631 kb on - strand at 2089.693 kb on + strand at 2089.693 kb on + strand at 2089.696 kb on + strand at 2089.696 kb on + strand at 2089.696 kb on + strand at 2090.069 kb on + strand, within Pf6N2E2_1768 at 2090.069 kb on + strand, within Pf6N2E2_1768 at 2090.070 kb on - strand, within Pf6N2E2_1768 at 2090.099 kb on - strand, within Pf6N2E2_1768 at 2090.099 kb on - strand, within Pf6N2E2_1768 at 2090.175 kb on - strand, within Pf6N2E2_1768 at 2090.363 kb on + strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.426 kb on - strand, within Pf6N2E2_1768 at 2090.476 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.843 kb on - strand at 2090.883 kb on - strand at 2090.883 kb on - strand at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.280 kb on + strand, within Pf6N2E2_1769 at 2091.303 kb on + strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.308 kb on - strand at 2091.388 kb on - strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.449 kb on + strand at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.905 kb on + strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.945 kb on - strand, within Pf6N2E2_1770
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,088,582 + -1.2 2,088,583 - -1.3 2,088,583 - +1.4 2,088,583 - +0.8 2,088,601 + +1.1 2,088,634 + +0.2 2,088,812 - +0.1 2,088,812 - +1.2 2,088,880 + Pf6N2E2_1767 0.19 +0.8 2,088,881 - Pf6N2E2_1767 0.19 -0.9 2,088,916 + Pf6N2E2_1767 0.23 +0.3 2,088,917 - Pf6N2E2_1767 0.23 +0.3 2,088,917 - Pf6N2E2_1767 0.23 -0.7 2,089,057 - Pf6N2E2_1767 0.42 -0.5 2,089,057 - Pf6N2E2_1767 0.42 -1.9 2,089,058 + Pf6N2E2_1767 0.42 -1.0 2,089,067 - Pf6N2E2_1767 0.43 -0.1 2,089,067 - Pf6N2E2_1767 0.43 -0.1 2,089,067 - Pf6N2E2_1767 0.43 +0.2 2,089,067 - Pf6N2E2_1767 0.43 -0.3 2,089,125 + Pf6N2E2_1767 0.51 -0.2 2,089,125 + Pf6N2E2_1767 0.51 +0.8 2,089,126 - Pf6N2E2_1767 0.51 -0.9 2,089,180 + Pf6N2E2_1767 0.58 +1.3 2,089,249 + Pf6N2E2_1767 0.67 +0.1 2,089,270 + Pf6N2E2_1767 0.70 +0.3 2,089,270 + Pf6N2E2_1767 0.70 -1.0 2,089,270 + Pf6N2E2_1767 0.70 +2.6 2,089,303 + Pf6N2E2_1767 0.74 +0.1 2,089,304 - Pf6N2E2_1767 0.74 +0.2 2,089,304 - Pf6N2E2_1767 0.74 +1.4 2,089,309 + Pf6N2E2_1767 0.75 -0.7 2,089,423 + Pf6N2E2_1767 0.90 -0.5 2,089,499 - +0.3 2,089,630 + +0.4 2,089,631 - +0.0 2,089,693 + +0.7 2,089,693 + -0.6 2,089,696 + +1.0 2,089,696 + -0.0 2,089,696 + +1.9 2,090,069 + Pf6N2E2_1768 0.36 -0.7 2,090,069 + Pf6N2E2_1768 0.36 +0.8 2,090,070 - Pf6N2E2_1768 0.36 -0.0 2,090,099 - Pf6N2E2_1768 0.38 -0.7 2,090,099 - Pf6N2E2_1768 0.38 -0.1 2,090,175 - Pf6N2E2_1768 0.43 -0.3 2,090,363 + Pf6N2E2_1768 0.56 +0.1 2,090,364 - Pf6N2E2_1768 0.57 +2.7 2,090,364 - Pf6N2E2_1768 0.57 +0.6 2,090,364 - Pf6N2E2_1768 0.57 -0.2 2,090,364 - Pf6N2E2_1768 0.57 -0.5 2,090,364 - Pf6N2E2_1768 0.57 -1.5 2,090,425 + Pf6N2E2_1768 0.61 -1.4 2,090,425 + Pf6N2E2_1768 0.61 -0.4 2,090,426 - Pf6N2E2_1768 0.61 +1.1 2,090,476 + Pf6N2E2_1768 0.64 -0.3 2,090,696 + Pf6N2E2_1768 0.80 +0.5 2,090,696 + Pf6N2E2_1768 0.80 +0.4 2,090,843 - -0.7 2,090,883 - +0.1 2,090,883 - +0.5 2,091,220 + Pf6N2E2_1769 0.70 -0.2 2,091,220 + Pf6N2E2_1769 0.70 -2.5 2,091,220 + Pf6N2E2_1769 0.70 -0.2 2,091,220 + Pf6N2E2_1769 0.70 -0.1 2,091,220 + Pf6N2E2_1769 0.70 +1.3 2,091,220 + Pf6N2E2_1769 0.70 -1.7 2,091,221 - Pf6N2E2_1769 0.71 -1.2 2,091,221 - Pf6N2E2_1769 0.71 -0.5 2,091,221 - Pf6N2E2_1769 0.71 +0.6 2,091,221 - Pf6N2E2_1769 0.71 -0.5 2,091,224 + Pf6N2E2_1769 0.72 -1.2 2,091,224 + Pf6N2E2_1769 0.72 -0.7 2,091,225 - Pf6N2E2_1769 0.72 +1.2 2,091,225 - Pf6N2E2_1769 0.72 -0.0 2,091,225 - Pf6N2E2_1769 0.72 +1.3 2,091,225 - Pf6N2E2_1769 0.72 -0.9 2,091,280 + Pf6N2E2_1769 0.88 -2.1 2,091,303 + -0.6 2,091,304 - +0.7 2,091,304 - +1.4 2,091,304 - +1.1 2,091,308 - -0.2 2,091,388 - +0.8 2,091,434 + -0.7 2,091,434 + -0.7 2,091,434 + +0.0 2,091,449 + -1.6 2,091,507 - Pf6N2E2_1770 0.13 -2.3 2,091,507 - Pf6N2E2_1770 0.13 -0.5 2,091,729 - Pf6N2E2_1770 0.34 -2.9 2,091,729 - Pf6N2E2_1770 0.34 +0.8 2,091,792 + Pf6N2E2_1770 0.40 -0.7 2,091,792 + Pf6N2E2_1770 0.40 -1.0 2,091,792 + Pf6N2E2_1770 0.40 -0.7 2,091,793 - Pf6N2E2_1770 0.40 +0.8 2,091,793 - Pf6N2E2_1770 0.40 -0.9 2,091,905 + Pf6N2E2_1770 0.50 +1.5 2,091,906 - Pf6N2E2_1770 0.50 +0.6 2,091,906 - Pf6N2E2_1770 0.50 -0.5 2,091,906 - Pf6N2E2_1770 0.50 +0.4 2,091,945 - Pf6N2E2_1770 0.54 -1.0
Or see this region's nucleotide sequence