Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1491

Experiment: L-Glutamine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1490 and Pf6N2E2_1491 are separated by 114 nucleotidesPf6N2E2_1491 and Pf6N2E2_1492 are separated by 45 nucleotidesPf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotides Pf6N2E2_1490 - hypothetical protein, at 1,743,433 to 1,743,597 _1490 Pf6N2E2_1491 - Cystathionine beta-lyase (EC 4.4.1.8), at 1,743,712 to 1,746,477 _1491 Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122 _1492 Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986 _1493 Position (kb) 1743 1744 1745 1746 1747Strain fitness (log2 ratio) -2 -1 0 1 2at 1742.804 kb on - strandat 1742.872 kb on + strandat 1742.875 kb on + strandat 1742.956 kb on + strandat 1743.080 kb on - strandat 1743.392 kb on - strandat 1743.405 kb on + strandat 1743.473 kb on - strand, within Pf6N2E2_1490at 1743.554 kb on + strand, within Pf6N2E2_1490at 1743.559 kb on - strand, within Pf6N2E2_1490at 1743.620 kb on + strandat 1743.938 kb on + strandat 1743.958 kb on + strandat 1744.155 kb on - strand, within Pf6N2E2_1491at 1744.271 kb on + strand, within Pf6N2E2_1491at 1744.272 kb on - strand, within Pf6N2E2_1491at 1744.344 kb on + strand, within Pf6N2E2_1491at 1744.345 kb on - strand, within Pf6N2E2_1491at 1744.345 kb on - strand, within Pf6N2E2_1491at 1744.513 kb on + strand, within Pf6N2E2_1491at 1744.563 kb on - strand, within Pf6N2E2_1491at 1744.563 kb on - strand, within Pf6N2E2_1491at 1744.605 kb on - strand, within Pf6N2E2_1491at 1744.727 kb on + strand, within Pf6N2E2_1491at 1744.728 kb on - strand, within Pf6N2E2_1491at 1744.985 kb on + strand, within Pf6N2E2_1491at 1744.986 kb on - strand, within Pf6N2E2_1491at 1745.431 kb on + strand, within Pf6N2E2_1491at 1745.431 kb on + strand, within Pf6N2E2_1491at 1745.431 kb on + strand, within Pf6N2E2_1491at 1745.431 kb on + strand, within Pf6N2E2_1491at 1745.432 kb on - strand, within Pf6N2E2_1491at 1745.534 kb on + strand, within Pf6N2E2_1491at 1745.534 kb on + strand, within Pf6N2E2_1491at 1745.534 kb on + strand, within Pf6N2E2_1491at 1745.535 kb on - strand, within Pf6N2E2_1491at 1745.535 kb on - strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.841 kb on - strand, within Pf6N2E2_1491at 1745.841 kb on - strand, within Pf6N2E2_1491at 1745.841 kb on - strand, within Pf6N2E2_1491at 1746.118 kb on + strand, within Pf6N2E2_1491at 1746.119 kb on - strand, within Pf6N2E2_1491at 1746.119 kb on - strand, within Pf6N2E2_1491at 1746.346 kb on - strandat 1746.463 kb on + strandat 1746.464 kb on - strandat 1746.534 kb on - strandat 1746.569 kb on + strandat 1746.570 kb on - strandat 1746.636 kb on - strand, within Pf6N2E2_1492at 1746.715 kb on - strand, within Pf6N2E2_1492at 1746.715 kb on - strand, within Pf6N2E2_1492at 1746.758 kb on + strand, within Pf6N2E2_1492at 1746.792 kb on - strand, within Pf6N2E2_1492at 1747.117 kb on - strandat 1747.191 kb on - strandat 1747.191 kb on - strandat 1747.191 kb on - strandat 1747.243 kb on + strand, within Pf6N2E2_1493at 1747.332 kb on - strand, within Pf6N2E2_1493at 1747.382 kb on - strand, within Pf6N2E2_1493

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N)
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1,742,804 - +1.0
1,742,872 + -0.2
1,742,875 + -0.5
1,742,956 + +0.3
1,743,080 - -0.3
1,743,392 - +0.2
1,743,405 + -2.2
1,743,473 - Pf6N2E2_1490 0.24 +1.0
1,743,554 + Pf6N2E2_1490 0.73 +0.4
1,743,559 - Pf6N2E2_1490 0.76 -0.1
1,743,620 + +0.2
1,743,938 + +1.2
1,743,958 + -0.3
1,744,155 - Pf6N2E2_1491 0.16 +0.7
1,744,271 + Pf6N2E2_1491 0.20 +2.1
1,744,272 - Pf6N2E2_1491 0.20 -0.2
1,744,344 + Pf6N2E2_1491 0.23 -0.1
1,744,345 - Pf6N2E2_1491 0.23 +0.3
1,744,345 - Pf6N2E2_1491 0.23 +0.5
1,744,513 + Pf6N2E2_1491 0.29 +0.3
1,744,563 - Pf6N2E2_1491 0.31 +1.7
1,744,563 - Pf6N2E2_1491 0.31 -0.1
1,744,605 - Pf6N2E2_1491 0.32 -1.2
1,744,727 + Pf6N2E2_1491 0.37 +0.0
1,744,728 - Pf6N2E2_1491 0.37 +0.1
1,744,985 + Pf6N2E2_1491 0.46 +1.0
1,744,986 - Pf6N2E2_1491 0.46 +0.4
1,745,431 + Pf6N2E2_1491 0.62 -1.8
1,745,431 + Pf6N2E2_1491 0.62 +0.3
1,745,431 + Pf6N2E2_1491 0.62 +0.3
1,745,431 + Pf6N2E2_1491 0.62 -1.3
1,745,432 - Pf6N2E2_1491 0.62 +0.2
1,745,534 + Pf6N2E2_1491 0.66 +0.4
1,745,534 + Pf6N2E2_1491 0.66 +0.2
1,745,534 + Pf6N2E2_1491 0.66 -0.6
1,745,535 - Pf6N2E2_1491 0.66 +0.2
1,745,535 - Pf6N2E2_1491 0.66 +1.7
1,745,840 + Pf6N2E2_1491 0.77 -1.3
1,745,840 + Pf6N2E2_1491 0.77 -0.3
1,745,840 + Pf6N2E2_1491 0.77 -0.1
1,745,840 + Pf6N2E2_1491 0.77 -0.2
1,745,841 - Pf6N2E2_1491 0.77 +0.7
1,745,841 - Pf6N2E2_1491 0.77 -0.9
1,745,841 - Pf6N2E2_1491 0.77 +1.0
1,746,118 + Pf6N2E2_1491 0.87 -1.3
1,746,119 - Pf6N2E2_1491 0.87 +0.6
1,746,119 - Pf6N2E2_1491 0.87 +0.7
1,746,346 - -0.3
1,746,463 + -1.0
1,746,464 - -1.3
1,746,534 - +0.0
1,746,569 + -1.7
1,746,570 - -0.6
1,746,636 - Pf6N2E2_1492 0.19 +2.0
1,746,715 - Pf6N2E2_1492 0.32 +1.3
1,746,715 - Pf6N2E2_1492 0.32 -1.0
1,746,758 + Pf6N2E2_1492 0.39 +1.0
1,746,792 - Pf6N2E2_1492 0.45 -0.1
1,747,117 - -1.5
1,747,191 - +2.6
1,747,191 - +0.2
1,747,191 - -0.5
1,747,243 + Pf6N2E2_1493 0.11 +0.2
1,747,332 - Pf6N2E2_1493 0.22 +0.2
1,747,382 - Pf6N2E2_1493 0.28 +0.2

Or see this region's nucleotide sequence