Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1490 and Pf6N2E2_1491 are separated by 114 nucleotides Pf6N2E2_1491 and Pf6N2E2_1492 are separated by 45 nucleotides Pf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotides
Pf6N2E2_1490 - hypothetical protein, at 1,743,433 to 1,743,597
_1490
Pf6N2E2_1491 - Cystathionine beta-lyase (EC 4.4.1.8), at 1,743,712 to 1,746,477
_1491
Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122
_1492
Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986
_1493
Position (kb)
1743
1744
1745
1746
1747 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1742.804 kb on - strand at 1742.872 kb on + strand at 1742.875 kb on + strand at 1742.956 kb on + strand at 1743.080 kb on - strand at 1743.392 kb on - strand at 1743.405 kb on + strand at 1743.473 kb on - strand, within Pf6N2E2_1490 at 1743.554 kb on + strand, within Pf6N2E2_1490 at 1743.559 kb on - strand, within Pf6N2E2_1490 at 1743.620 kb on + strand at 1743.938 kb on + strand at 1743.958 kb on + strand at 1744.155 kb on - strand, within Pf6N2E2_1491 at 1744.271 kb on + strand, within Pf6N2E2_1491 at 1744.272 kb on - strand, within Pf6N2E2_1491 at 1744.344 kb on + strand, within Pf6N2E2_1491 at 1744.345 kb on - strand, within Pf6N2E2_1491 at 1744.345 kb on - strand, within Pf6N2E2_1491 at 1744.513 kb on + strand, within Pf6N2E2_1491 at 1744.563 kb on - strand, within Pf6N2E2_1491 at 1744.563 kb on - strand, within Pf6N2E2_1491 at 1744.605 kb on - strand, within Pf6N2E2_1491 at 1744.727 kb on + strand, within Pf6N2E2_1491 at 1744.728 kb on - strand, within Pf6N2E2_1491 at 1744.985 kb on + strand, within Pf6N2E2_1491 at 1744.986 kb on - strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.432 kb on - strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.535 kb on - strand, within Pf6N2E2_1491 at 1745.535 kb on - strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1746.118 kb on + strand, within Pf6N2E2_1491 at 1746.119 kb on - strand, within Pf6N2E2_1491 at 1746.119 kb on - strand, within Pf6N2E2_1491 at 1746.346 kb on - strand at 1746.463 kb on + strand at 1746.464 kb on - strand at 1746.534 kb on - strand at 1746.569 kb on + strand at 1746.570 kb on - strand at 1746.636 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.758 kb on + strand, within Pf6N2E2_1492 at 1746.792 kb on - strand, within Pf6N2E2_1492 at 1747.117 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.243 kb on + strand, within Pf6N2E2_1493 at 1747.332 kb on - strand, within Pf6N2E2_1493 at 1747.382 kb on - strand, within Pf6N2E2_1493
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,742,804 - +1.0 1,742,872 + -0.2 1,742,875 + -0.5 1,742,956 + +0.3 1,743,080 - -0.3 1,743,392 - +0.2 1,743,405 + -2.2 1,743,473 - Pf6N2E2_1490 0.24 +1.0 1,743,554 + Pf6N2E2_1490 0.73 +0.4 1,743,559 - Pf6N2E2_1490 0.76 -0.1 1,743,620 + +0.2 1,743,938 + +1.2 1,743,958 + -0.3 1,744,155 - Pf6N2E2_1491 0.16 +0.7 1,744,271 + Pf6N2E2_1491 0.20 +2.1 1,744,272 - Pf6N2E2_1491 0.20 -0.2 1,744,344 + Pf6N2E2_1491 0.23 -0.1 1,744,345 - Pf6N2E2_1491 0.23 +0.3 1,744,345 - Pf6N2E2_1491 0.23 +0.5 1,744,513 + Pf6N2E2_1491 0.29 +0.3 1,744,563 - Pf6N2E2_1491 0.31 +1.7 1,744,563 - Pf6N2E2_1491 0.31 -0.1 1,744,605 - Pf6N2E2_1491 0.32 -1.2 1,744,727 + Pf6N2E2_1491 0.37 +0.0 1,744,728 - Pf6N2E2_1491 0.37 +0.1 1,744,985 + Pf6N2E2_1491 0.46 +1.0 1,744,986 - Pf6N2E2_1491 0.46 +0.4 1,745,431 + Pf6N2E2_1491 0.62 -1.8 1,745,431 + Pf6N2E2_1491 0.62 +0.3 1,745,431 + Pf6N2E2_1491 0.62 +0.3 1,745,431 + Pf6N2E2_1491 0.62 -1.3 1,745,432 - Pf6N2E2_1491 0.62 +0.2 1,745,534 + Pf6N2E2_1491 0.66 +0.4 1,745,534 + Pf6N2E2_1491 0.66 +0.2 1,745,534 + Pf6N2E2_1491 0.66 -0.6 1,745,535 - Pf6N2E2_1491 0.66 +0.2 1,745,535 - Pf6N2E2_1491 0.66 +1.7 1,745,840 + Pf6N2E2_1491 0.77 -1.3 1,745,840 + Pf6N2E2_1491 0.77 -0.3 1,745,840 + Pf6N2E2_1491 0.77 -0.1 1,745,840 + Pf6N2E2_1491 0.77 -0.2 1,745,841 - Pf6N2E2_1491 0.77 +0.7 1,745,841 - Pf6N2E2_1491 0.77 -0.9 1,745,841 - Pf6N2E2_1491 0.77 +1.0 1,746,118 + Pf6N2E2_1491 0.87 -1.3 1,746,119 - Pf6N2E2_1491 0.87 +0.6 1,746,119 - Pf6N2E2_1491 0.87 +0.7 1,746,346 - -0.3 1,746,463 + -1.0 1,746,464 - -1.3 1,746,534 - +0.0 1,746,569 + -1.7 1,746,570 - -0.6 1,746,636 - Pf6N2E2_1492 0.19 +2.0 1,746,715 - Pf6N2E2_1492 0.32 +1.3 1,746,715 - Pf6N2E2_1492 0.32 -1.0 1,746,758 + Pf6N2E2_1492 0.39 +1.0 1,746,792 - Pf6N2E2_1492 0.45 -0.1 1,747,117 - -1.5 1,747,191 - +2.6 1,747,191 - +0.2 1,747,191 - -0.5 1,747,243 + Pf6N2E2_1493 0.11 +0.2 1,747,332 - Pf6N2E2_1493 0.22 +0.2 1,747,382 - Pf6N2E2_1493 0.28 +0.2
Or see this region's nucleotide sequence