Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5508 and Pf6N2E2_5509 are separated by 125 nucleotides Pf6N2E2_5509 and Pf6N2E2_5510 are separated by 4 nucleotides Pf6N2E2_5510 and Pf6N2E2_5511 overlap by 4 nucleotides Pf6N2E2_5511 and Pf6N2E2_5512 are separated by 16 nucleotides
Pf6N2E2_5508 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 6,325,636 to 6,326,484
_5508
Pf6N2E2_5509 - FIG00955586: hypothetical protein, at 6,326,610 to 6,326,783
_5509
Pf6N2E2_5510 - FIG00955324: hypothetical protein, at 6,326,788 to 6,327,783
_5510
Pf6N2E2_5511 - FIG00955875: hypothetical protein, at 6,327,780 to 6,328,547
_5511
Pf6N2E2_5512 - glycosyl transferase, group 1 family protein, at 6,328,564 to 6,329,655
_5512
Position (kb)
6326
6327
6328 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6325.872 kb on + strand, within Pf6N2E2_5508 at 6326.183 kb on - strand, within Pf6N2E2_5508 at 6326.399 kb on + strand, within Pf6N2E2_5508 at 6326.528 kb on - strand at 6326.540 kb on + strand at 6326.541 kb on - strand at 6326.541 kb on - strand at 6326.541 kb on - strand at 6326.541 kb on - strand at 6326.749 kb on + strand, within Pf6N2E2_5509 at 6326.749 kb on + strand, within Pf6N2E2_5509 at 6326.750 kb on - strand, within Pf6N2E2_5509 at 6326.750 kb on - strand, within Pf6N2E2_5509 at 6326.750 kb on - strand, within Pf6N2E2_5509 at 6326.750 kb on - strand, within Pf6N2E2_5509 at 6326.750 kb on - strand, within Pf6N2E2_5509 at 6326.806 kb on + strand at 6326.870 kb on + strand at 6326.871 kb on - strand at 6326.871 kb on - strand at 6326.876 kb on + strand at 6326.877 kb on - strand at 6326.877 kb on - strand at 6326.879 kb on + strand at 6326.879 kb on + strand at 6326.879 kb on + strand at 6326.879 kb on + strand at 6326.879 kb on + strand at 6326.880 kb on - strand at 6327.286 kb on + strand, within Pf6N2E2_5510 at 6327.287 kb on - strand, within Pf6N2E2_5510 at 6327.380 kb on + strand, within Pf6N2E2_5510 at 6327.381 kb on - strand, within Pf6N2E2_5510 at 6327.883 kb on + strand, within Pf6N2E2_5511 at 6327.884 kb on - strand, within Pf6N2E2_5511 at 6327.884 kb on - strand, within Pf6N2E2_5511 at 6327.900 kb on - strand, within Pf6N2E2_5511 at 6328.064 kb on - strand, within Pf6N2E2_5511 at 6328.087 kb on + strand, within Pf6N2E2_5511 at 6328.087 kb on + strand, within Pf6N2E2_5511 at 6328.087 kb on + strand, within Pf6N2E2_5511 at 6328.087 kb on + strand, within Pf6N2E2_5511 at 6328.087 kb on + strand, within Pf6N2E2_5511 at 6328.175 kb on - strand, within Pf6N2E2_5511 at 6328.237 kb on + strand, within Pf6N2E2_5511 at 6328.237 kb on + strand, within Pf6N2E2_5511 at 6328.238 kb on - strand, within Pf6N2E2_5511 at 6328.238 kb on - strand, within Pf6N2E2_5511 at 6328.238 kb on - strand, within Pf6N2E2_5511 at 6328.238 kb on - strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.258 kb on + strand, within Pf6N2E2_5511 at 6328.259 kb on - strand, within Pf6N2E2_5511 at 6328.259 kb on - strand, within Pf6N2E2_5511 at 6328.259 kb on - strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.318 kb on + strand, within Pf6N2E2_5511 at 6328.319 kb on - strand, within Pf6N2E2_5511 at 6328.319 kb on - strand, within Pf6N2E2_5511 at 6328.319 kb on - strand, within Pf6N2E2_5511 at 6328.319 kb on - strand, within Pf6N2E2_5511 at 6328.319 kb on - strand, within Pf6N2E2_5511 at 6328.319 kb on - strand, within Pf6N2E2_5511 at 6328.444 kb on - strand, within Pf6N2E2_5511 at 6328.444 kb on - strand, within Pf6N2E2_5511 at 6328.484 kb on - strand at 6328.524 kb on + strand at 6328.525 kb on - strand at 6328.525 kb on - strand at 6328.607 kb on + strand at 6328.607 kb on + strand at 6328.607 kb on + strand at 6328.607 kb on + strand at 6328.608 kb on - strand at 6328.608 kb on - strand at 6328.610 kb on + strand at 6328.611 kb on - strand at 6328.611 kb on - strand at 6328.649 kb on + strand at 6328.649 kb on + strand at 6328.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 6,325,872 + Pf6N2E2_5508 0.28 +0.2 6,326,183 - Pf6N2E2_5508 0.64 -0.2 6,326,399 + Pf6N2E2_5508 0.90 +0.8 6,326,528 - -1.0 6,326,540 + +1.1 6,326,541 - -0.2 6,326,541 - -0.5 6,326,541 - -0.1 6,326,541 - +0.1 6,326,749 + Pf6N2E2_5509 0.80 +2.0 6,326,749 + Pf6N2E2_5509 0.80 +0.5 6,326,750 - Pf6N2E2_5509 0.80 +0.9 6,326,750 - Pf6N2E2_5509 0.80 +0.8 6,326,750 - Pf6N2E2_5509 0.80 +0.5 6,326,750 - Pf6N2E2_5509 0.80 -0.6 6,326,750 - Pf6N2E2_5509 0.80 +0.3 6,326,806 + +0.4 6,326,870 + +1.2 6,326,871 - +0.6 6,326,871 - -0.9 6,326,876 + -0.8 6,326,877 - +1.1 6,326,877 - -0.2 6,326,879 + -0.2 6,326,879 + +0.4 6,326,879 + -1.2 6,326,879 + -0.1 6,326,879 + -1.1 6,326,880 - +0.1 6,327,286 + Pf6N2E2_5510 0.50 -1.9 6,327,287 - Pf6N2E2_5510 0.50 -0.4 6,327,380 + Pf6N2E2_5510 0.59 -1.3 6,327,381 - Pf6N2E2_5510 0.60 +0.5 6,327,883 + Pf6N2E2_5511 0.13 -0.0 6,327,884 - Pf6N2E2_5511 0.14 +0.1 6,327,884 - Pf6N2E2_5511 0.14 -1.8 6,327,900 - Pf6N2E2_5511 0.16 +0.3 6,328,064 - Pf6N2E2_5511 0.37 +0.6 6,328,087 + Pf6N2E2_5511 0.40 -0.7 6,328,087 + Pf6N2E2_5511 0.40 +0.0 6,328,087 + Pf6N2E2_5511 0.40 -0.7 6,328,087 + Pf6N2E2_5511 0.40 +0.3 6,328,087 + Pf6N2E2_5511 0.40 -0.4 6,328,175 - Pf6N2E2_5511 0.51 +0.4 6,328,237 + Pf6N2E2_5511 0.60 +0.5 6,328,237 + Pf6N2E2_5511 0.60 +1.3 6,328,238 - Pf6N2E2_5511 0.60 +2.4 6,328,238 - Pf6N2E2_5511 0.60 -1.1 6,328,238 - Pf6N2E2_5511 0.60 -1.8 6,328,238 - Pf6N2E2_5511 0.60 +0.3 6,328,258 + Pf6N2E2_5511 0.62 +0.6 6,328,258 + Pf6N2E2_5511 0.62 +0.2 6,328,258 + Pf6N2E2_5511 0.62 -0.2 6,328,258 + Pf6N2E2_5511 0.62 -0.6 6,328,258 + Pf6N2E2_5511 0.62 -1.3 6,328,258 + Pf6N2E2_5511 0.62 +0.9 6,328,258 + Pf6N2E2_5511 0.62 -1.2 6,328,259 - Pf6N2E2_5511 0.62 -0.5 6,328,259 - Pf6N2E2_5511 0.62 +1.0 6,328,259 - Pf6N2E2_5511 0.62 +1.3 6,328,318 + Pf6N2E2_5511 0.70 -0.9 6,328,318 + Pf6N2E2_5511 0.70 -0.4 6,328,318 + Pf6N2E2_5511 0.70 -0.3 6,328,318 + Pf6N2E2_5511 0.70 +0.3 6,328,318 + Pf6N2E2_5511 0.70 -3.0 6,328,318 + Pf6N2E2_5511 0.70 -0.5 6,328,318 + Pf6N2E2_5511 0.70 -0.2 6,328,319 - Pf6N2E2_5511 0.70 -0.5 6,328,319 - Pf6N2E2_5511 0.70 +0.2 6,328,319 - Pf6N2E2_5511 0.70 -0.2 6,328,319 - Pf6N2E2_5511 0.70 -0.1 6,328,319 - Pf6N2E2_5511 0.70 -0.0 6,328,319 - Pf6N2E2_5511 0.70 -2.1 6,328,444 - Pf6N2E2_5511 0.86 +0.3 6,328,444 - Pf6N2E2_5511 0.86 -0.3 6,328,484 - -0.8 6,328,524 + -0.4 6,328,525 - -0.8 6,328,525 - -0.8 6,328,607 + +0.3 6,328,607 + -0.1 6,328,607 + +0.3 6,328,607 + -0.7 6,328,608 - +0.9 6,328,608 - +1.4 6,328,610 + +0.2 6,328,611 - -0.1 6,328,611 - +0.3 6,328,649 + -1.8 6,328,649 + +0.0 6,328,650 - +0.6
Or see this region's nucleotide sequence