Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_366 and Pf6N2E2_367 overlap by 4 nucleotides Pf6N2E2_367 and Pf6N2E2_368 are separated by 254 nucleotides
Pf6N2E2_366 - Opine oxidase subunit B, at 432,477 to 433,631
_366
Pf6N2E2_367 - Acetylornithine deacetylase (EC 3.5.1.16), at 433,628 to 434,785
_367
Pf6N2E2_368 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 435,040 to 436,497
_368
Position (kb) 433
434
435 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 432.772 kb on + strand, within Pf6N2E2_366 at 432.895 kb on - strand, within Pf6N2E2_366 at 433.177 kb on - strand, within Pf6N2E2_366 at 433.177 kb on - strand, within Pf6N2E2_366 at 433.204 kb on - strand, within Pf6N2E2_366 at 433.204 kb on - strand, within Pf6N2E2_366 at 433.231 kb on - strand, within Pf6N2E2_366 at 433.231 kb on - strand, within Pf6N2E2_366 at 433.256 kb on + strand, within Pf6N2E2_366 at 433.256 kb on + strand, within Pf6N2E2_366 at 433.311 kb on + strand, within Pf6N2E2_366 at 433.311 kb on + strand, within Pf6N2E2_366 at 433.311 kb on + strand, within Pf6N2E2_366 at 433.312 kb on - strand, within Pf6N2E2_366 at 433.312 kb on - strand, within Pf6N2E2_366 at 433.416 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.642 kb on - strand at 433.917 kb on - strand, within Pf6N2E2_367 at 433.917 kb on - strand, within Pf6N2E2_367 at 434.318 kb on + strand, within Pf6N2E2_367 at 434.528 kb on + strand, within Pf6N2E2_367 at 434.783 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.800 kb on + strand at 434.800 kb on + strand at 434.800 kb on + strand at 434.801 kb on - strand at 434.801 kb on - strand at 434.801 kb on - strand at 434.893 kb on + strand at 434.893 kb on + strand at 434.893 kb on + strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 435.007 kb on + strand at 435.018 kb on + strand at 435.088 kb on + strand at 435.089 kb on - strand at 435.174 kb on - strand at 435.427 kb on + strand, within Pf6N2E2_368
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 432,772 + Pf6N2E2_366 0.26 +1.1 432,895 - Pf6N2E2_366 0.36 -0.8 433,177 - Pf6N2E2_366 0.61 -0.1 433,177 - Pf6N2E2_366 0.61 +0.2 433,204 - Pf6N2E2_366 0.63 +0.6 433,204 - Pf6N2E2_366 0.63 +0.1 433,231 - Pf6N2E2_366 0.65 -0.1 433,231 - Pf6N2E2_366 0.65 +0.4 433,256 + Pf6N2E2_366 0.67 +1.7 433,256 + Pf6N2E2_366 0.67 +0.3 433,311 + Pf6N2E2_366 0.72 -1.5 433,311 + Pf6N2E2_366 0.72 +0.1 433,311 + Pf6N2E2_366 0.72 -0.2 433,312 - Pf6N2E2_366 0.72 +0.4 433,312 - Pf6N2E2_366 0.72 -0.3 433,416 + Pf6N2E2_366 0.81 +0.1 433,470 + Pf6N2E2_366 0.86 -0.1 433,470 + Pf6N2E2_366 0.86 -0.2 433,470 + Pf6N2E2_366 0.86 -0.4 433,470 + Pf6N2E2_366 0.86 +0.6 433,470 + Pf6N2E2_366 0.86 +2.0 433,470 + Pf6N2E2_366 0.86 +0.3 433,471 - Pf6N2E2_366 0.86 -1.3 433,471 - Pf6N2E2_366 0.86 +1.5 433,471 - Pf6N2E2_366 0.86 -2.0 433,471 - Pf6N2E2_366 0.86 +0.2 433,471 - Pf6N2E2_366 0.86 -0.3 433,471 - Pf6N2E2_366 0.86 -1.7 433,474 - Pf6N2E2_366 0.86 -0.1 433,474 - Pf6N2E2_366 0.86 -1.5 433,474 - Pf6N2E2_366 0.86 -0.1 433,474 - Pf6N2E2_366 0.86 +1.5 433,642 - +0.6 433,917 - Pf6N2E2_367 0.25 -3.1 433,917 - Pf6N2E2_367 0.25 -0.2 434,318 + Pf6N2E2_367 0.60 -0.5 434,528 + Pf6N2E2_367 0.78 -1.5 434,783 + +0.9 434,792 + +0.1 434,792 + +0.4 434,792 + -1.1 434,792 + +1.3 434,792 + +1.5 434,792 + -0.5 434,793 - -1.3 434,793 - -0.9 434,793 - +0.7 434,793 - +0.6 434,793 - +0.5 434,800 + -0.9 434,800 + +0.0 434,800 + +0.2 434,801 - +0.8 434,801 - +0.9 434,801 - -0.5 434,893 + -0.4 434,893 + -0.6 434,893 + -0.7 434,894 - +0.6 434,894 - -0.6 434,894 - -0.0 434,894 - +0.9 434,894 - -0.1 434,894 - +0.6 435,007 + +0.1 435,018 + -0.8 435,088 + +0.4 435,089 - -0.6 435,174 - -1.4 435,427 + Pf6N2E2_368 0.27 -0.5
Or see this region's nucleotide sequence