Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1716 and Pf6N2E2_1717 are separated by 651 nucleotides Pf6N2E2_1717 and Pf6N2E2_1718 overlap by 4 nucleotides
Pf6N2E2_1716 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 2,023,609 to 2,024,961
_1716
Pf6N2E2_1717 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 2,025,613 to 2,027,082
_1717
Pf6N2E2_1718 - Anthranilate synthase, amidotransferase component (EC 4.1.3.27), at 2,027,079 to 2,027,663
_1718
Position (kb)
2025
2026
2027
2028 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2024.672 kb on + strand, within Pf6N2E2_1716 at 2024.907 kb on - strand at 2025.025 kb on - strand at 2025.027 kb on - strand at 2025.093 kb on - strand at 2025.097 kb on - strand at 2025.109 kb on - strand at 2025.347 kb on + strand at 2025.606 kb on - strand at 2025.683 kb on + strand at 2025.685 kb on + strand at 2025.914 kb on + strand, within Pf6N2E2_1717 at 2026.009 kb on + strand, within Pf6N2E2_1717 at 2026.070 kb on - strand, within Pf6N2E2_1717 at 2026.070 kb on - strand, within Pf6N2E2_1717 at 2026.070 kb on - strand, within Pf6N2E2_1717 at 2026.100 kb on - strand, within Pf6N2E2_1717 at 2026.153 kb on - strand, within Pf6N2E2_1717 at 2026.336 kb on + strand, within Pf6N2E2_1717 at 2026.429 kb on + strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.448 kb on + strand, within Pf6N2E2_1717 at 2026.448 kb on + strand, within Pf6N2E2_1717 at 2026.448 kb on + strand, within Pf6N2E2_1717 at 2026.706 kb on - strand, within Pf6N2E2_1717 at 2026.706 kb on - strand, within Pf6N2E2_1717 at 2026.717 kb on + strand, within Pf6N2E2_1717 at 2026.718 kb on - strand, within Pf6N2E2_1717 at 2026.873 kb on + strand, within Pf6N2E2_1717 at 2026.874 kb on - strand, within Pf6N2E2_1717 at 2026.874 kb on - strand, within Pf6N2E2_1717 at 2026.956 kb on + strand at 2026.957 kb on - strand at 2026.960 kb on + strand at 2026.960 kb on + strand at 2026.961 kb on - strand at 2027.029 kb on + strand at 2027.030 kb on - strand at 2027.030 kb on - strand at 2027.030 kb on - strand at 2027.030 kb on - strand at 2027.086 kb on + strand at 2027.087 kb on - strand at 2027.134 kb on - strand at 2027.265 kb on + strand, within Pf6N2E2_1718 at 2027.539 kb on - strand, within Pf6N2E2_1718 at 2027.539 kb on - strand, within Pf6N2E2_1718 at 2027.723 kb on + strand at 2027.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,024,672 + Pf6N2E2_1716 0.79 +1.4 2,024,907 - +0.3 2,025,025 - -1.2 2,025,027 - -0.1 2,025,093 - -0.8 2,025,097 - -0.1 2,025,109 - -0.6 2,025,347 + -0.5 2,025,606 - +0.3 2,025,683 + -0.9 2,025,685 + -2.6 2,025,914 + Pf6N2E2_1717 0.20 -1.7 2,026,009 + Pf6N2E2_1717 0.27 -0.9 2,026,070 - Pf6N2E2_1717 0.31 -0.9 2,026,070 - Pf6N2E2_1717 0.31 -1.4 2,026,070 - Pf6N2E2_1717 0.31 -0.9 2,026,100 - Pf6N2E2_1717 0.33 -1.0 2,026,153 - Pf6N2E2_1717 0.37 -1.6 2,026,336 + Pf6N2E2_1717 0.49 -1.1 2,026,429 + Pf6N2E2_1717 0.56 -1.2 2,026,430 - Pf6N2E2_1717 0.56 -2.1 2,026,430 - Pf6N2E2_1717 0.56 -2.1 2,026,430 - Pf6N2E2_1717 0.56 -2.0 2,026,430 - Pf6N2E2_1717 0.56 -2.6 2,026,435 + Pf6N2E2_1717 0.56 +2.0 2,026,435 + Pf6N2E2_1717 0.56 -2.7 2,026,435 + Pf6N2E2_1717 0.56 -0.6 2,026,435 + Pf6N2E2_1717 0.56 -2.6 2,026,435 + Pf6N2E2_1717 0.56 -0.8 2,026,436 - Pf6N2E2_1717 0.56 -0.9 2,026,436 - Pf6N2E2_1717 0.56 -3.5 2,026,436 - Pf6N2E2_1717 0.56 -1.9 2,026,436 - Pf6N2E2_1717 0.56 -1.4 2,026,448 + Pf6N2E2_1717 0.57 -0.6 2,026,448 + Pf6N2E2_1717 0.57 -0.9 2,026,448 + Pf6N2E2_1717 0.57 -2.9 2,026,706 - Pf6N2E2_1717 0.74 -0.3 2,026,706 - Pf6N2E2_1717 0.74 -1.6 2,026,717 + Pf6N2E2_1717 0.75 -1.5 2,026,718 - Pf6N2E2_1717 0.75 -1.6 2,026,873 + Pf6N2E2_1717 0.86 -2.6 2,026,874 - Pf6N2E2_1717 0.86 -1.1 2,026,874 - Pf6N2E2_1717 0.86 -1.1 2,026,956 + -0.6 2,026,957 - -1.3 2,026,960 + -2.1 2,026,960 + -1.3 2,026,961 - -0.8 2,027,029 + -3.6 2,027,030 - -1.3 2,027,030 - -1.6 2,027,030 - -3.0 2,027,030 - +0.4 2,027,086 + -1.7 2,027,087 - +2.0 2,027,134 - -0.5 2,027,265 + Pf6N2E2_1718 0.32 -1.5 2,027,539 - Pf6N2E2_1718 0.79 -0.8 2,027,539 - Pf6N2E2_1718 0.79 +0.0 2,027,723 + +1.0 2,027,723 + -0.3
Or see this region's nucleotide sequence