Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1522 and Pf6N2E2_1523 overlap by 11 nucleotides Pf6N2E2_1523 and Pf6N2E2_1524 are separated by 246 nucleotides Pf6N2E2_1524 and Pf6N2E2_1525 are separated by 51 nucleotides
Pf6N2E2_1522 - Alcohol dehydrogenase (EC 1.1.1.1), at 1,780,102 to 1,781,085
_1522
Pf6N2E2_1523 - Universal stress protein family 5, at 1,781,075 to 1,781,968
_1523
Pf6N2E2_1524 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 1,782,215 to 1,782,778
_1524
Pf6N2E2_1525 - Universal stress protein family 5, at 1,782,830 to 1,783,741
_1525
Position (kb)
1781
1782 Strain fitness (log2 ratio)
-2
-1
0
1 at 1780.465 kb on + strand, within Pf6N2E2_1522 at 1780.604 kb on - strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.667 kb on - strand, within Pf6N2E2_1522 at 1781.006 kb on - strand at 1781.072 kb on + strand at 1781.073 kb on - strand at 1781.073 kb on - strand at 1781.112 kb on + strand at 1781.112 kb on + strand at 1781.113 kb on - strand at 1781.290 kb on + strand, within Pf6N2E2_1523 at 1781.291 kb on - strand, within Pf6N2E2_1523 at 1781.397 kb on + strand, within Pf6N2E2_1523 at 1781.398 kb on - strand, within Pf6N2E2_1523 at 1781.626 kb on + strand, within Pf6N2E2_1523 at 1781.703 kb on - strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.823 kb on - strand, within Pf6N2E2_1523 at 1781.823 kb on - strand, within Pf6N2E2_1523 at 1781.946 kb on - strand at 1782.476 kb on + strand, within Pf6N2E2_1524 at 1782.476 kb on + strand, within Pf6N2E2_1524 at 1782.476 kb on + strand, within Pf6N2E2_1524 at 1782.477 kb on - strand, within Pf6N2E2_1524 at 1782.477 kb on - strand, within Pf6N2E2_1524 at 1782.477 kb on - strand, within Pf6N2E2_1524 at 1782.662 kb on + strand, within Pf6N2E2_1524 at 1782.662 kb on + strand, within Pf6N2E2_1524 at 1782.663 kb on - strand, within Pf6N2E2_1524 at 1782.663 kb on - strand, within Pf6N2E2_1524 at 1782.771 kb on - strand at 1782.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,780,465 + Pf6N2E2_1522 0.37 +0.6 1,780,604 - Pf6N2E2_1522 0.51 -2.4 1,780,666 + Pf6N2E2_1522 0.57 +1.1 1,780,666 + Pf6N2E2_1522 0.57 +0.5 1,780,666 + Pf6N2E2_1522 0.57 +0.1 1,780,666 + Pf6N2E2_1522 0.57 +0.4 1,780,667 - Pf6N2E2_1522 0.57 +1.3 1,781,006 - -0.4 1,781,072 + -0.6 1,781,073 - -0.1 1,781,073 - -1.5 1,781,112 + +0.9 1,781,112 + +0.7 1,781,113 - -0.0 1,781,290 + Pf6N2E2_1523 0.24 -1.4 1,781,291 - Pf6N2E2_1523 0.24 -0.4 1,781,397 + Pf6N2E2_1523 0.36 +0.6 1,781,398 - Pf6N2E2_1523 0.36 -0.9 1,781,626 + Pf6N2E2_1523 0.62 +0.6 1,781,703 - Pf6N2E2_1523 0.70 -0.5 1,781,822 + Pf6N2E2_1523 0.84 -1.6 1,781,822 + Pf6N2E2_1523 0.84 -0.4 1,781,822 + Pf6N2E2_1523 0.84 -1.3 1,781,823 - Pf6N2E2_1523 0.84 -0.5 1,781,823 - Pf6N2E2_1523 0.84 -2.3 1,781,946 - -1.0 1,782,476 + Pf6N2E2_1524 0.46 -0.3 1,782,476 + Pf6N2E2_1524 0.46 +0.6 1,782,476 + Pf6N2E2_1524 0.46 +1.2 1,782,477 - Pf6N2E2_1524 0.46 +1.3 1,782,477 - Pf6N2E2_1524 0.46 -0.6 1,782,477 - Pf6N2E2_1524 0.46 -0.0 1,782,662 + Pf6N2E2_1524 0.79 +0.5 1,782,662 + Pf6N2E2_1524 0.79 -1.1 1,782,663 - Pf6N2E2_1524 0.79 -0.4 1,782,663 - Pf6N2E2_1524 0.79 -1.0 1,782,771 - -0.5 1,782,805 - -0.2
Or see this region's nucleotide sequence