Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_60 and Pf6N2E2_61 are separated by 69 nucleotides Pf6N2E2_61 and Pf6N2E2_62 overlap by 31 nucleotides Pf6N2E2_62 and Pf6N2E2_63 are separated by 157 nucleotides
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Pf6N2E2_61 - Cys regulon transcriptional activator CysB, at 101,960 to 102,238
_61
Pf6N2E2_62 - Cys regulon transcriptional activator CysB, at 102,208 to 102,852
_62
Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428
_63
Position (kb)
101
102
103 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 101.101 kb on + strand, within Pf6N2E2_60 at 101.101 kb on + strand, within Pf6N2E2_60 at 101.241 kb on - strand, within Pf6N2E2_60 at 101.331 kb on + strand, within Pf6N2E2_60 at 101.649 kb on - strand at 101.661 kb on + strand at 101.662 kb on - strand at 101.662 kb on - strand at 101.891 kb on + strand at 101.921 kb on + strand at 101.922 kb on - strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.962 kb on - strand at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.078 kb on - strand, within Pf6N2E2_61 at 102.168 kb on - strand, within Pf6N2E2_61 at 102.180 kb on + strand, within Pf6N2E2_61 at 102.250 kb on + strand at 102.393 kb on - strand at 102.455 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.823 kb on + strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.826 kb on - strand at 102.826 kb on - strand at 102.920 kb on + strand at 102.921 kb on - strand at 102.929 kb on - strand at 102.955 kb on + strand at 102.981 kb on + strand at 102.982 kb on - strand at 103.028 kb on + strand at 103.028 kb on + strand at 103.088 kb on + strand at 103.088 kb on + strand at 103.088 kb on + strand at 103.206 kb on + strand, within Pf6N2E2_63 at 103.207 kb on - strand, within Pf6N2E2_63
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 101,101 + Pf6N2E2_60 0.76 +0.2 101,101 + Pf6N2E2_60 0.76 -1.2 101,241 - Pf6N2E2_60 0.80 -1.5 101,331 + Pf6N2E2_60 0.83 +1.5 101,649 - -1.0 101,661 + +1.8 101,662 - +0.1 101,662 - +0.8 101,891 + +0.5 101,921 + +0.7 101,922 - +1.2 101,961 + +0.4 101,961 + -0.2 101,961 + +0.1 101,961 + +0.2 101,961 + +0.7 101,962 - +2.2 102,052 + Pf6N2E2_61 0.33 +1.4 102,052 + Pf6N2E2_61 0.33 +0.6 102,052 + Pf6N2E2_61 0.33 -0.1 102,052 + Pf6N2E2_61 0.33 +0.0 102,052 + Pf6N2E2_61 0.33 +0.2 102,052 + Pf6N2E2_61 0.33 +1.7 102,052 + Pf6N2E2_61 0.33 -0.9 102,052 + Pf6N2E2_61 0.33 +0.1 102,053 - Pf6N2E2_61 0.33 +1.9 102,053 - Pf6N2E2_61 0.33 +1.0 102,053 - Pf6N2E2_61 0.33 +1.8 102,053 - Pf6N2E2_61 0.33 +2.0 102,053 - Pf6N2E2_61 0.33 +2.2 102,054 + Pf6N2E2_61 0.34 +1.0 102,054 + Pf6N2E2_61 0.34 +0.6 102,054 + Pf6N2E2_61 0.34 +0.5 102,055 - Pf6N2E2_61 0.34 +0.5 102,055 - Pf6N2E2_61 0.34 +0.6 102,055 - Pf6N2E2_61 0.34 +1.1 102,055 - Pf6N2E2_61 0.34 +2.0 102,059 + Pf6N2E2_61 0.35 -0.4 102,059 + Pf6N2E2_61 0.35 +1.0 102,060 - Pf6N2E2_61 0.36 +1.7 102,060 - Pf6N2E2_61 0.36 +1.0 102,077 + Pf6N2E2_61 0.42 +2.0 102,077 + Pf6N2E2_61 0.42 -0.1 102,077 + Pf6N2E2_61 0.42 +0.9 102,077 + Pf6N2E2_61 0.42 +0.4 102,078 - Pf6N2E2_61 0.42 +1.4 102,168 - Pf6N2E2_61 0.75 +1.9 102,180 + Pf6N2E2_61 0.79 +1.6 102,250 + +0.8 102,393 - +0.2 102,455 + Pf6N2E2_62 0.38 +0.2 102,550 + Pf6N2E2_62 0.53 +1.3 102,550 + Pf6N2E2_62 0.53 +0.2 102,551 - Pf6N2E2_62 0.53 -0.2 102,551 - Pf6N2E2_62 0.53 -0.6 102,551 - Pf6N2E2_62 0.53 -1.1 102,551 - Pf6N2E2_62 0.53 -0.0 102,823 + +1.4 102,824 - -0.9 102,824 - +0.7 102,824 - +1.2 102,824 - -2.2 102,825 + +0.8 102,825 + +0.8 102,825 + +1.1 102,825 + +1.3 102,826 - -2.5 102,826 - -1.0 102,920 + +2.7 102,921 - -0.5 102,929 - -2.1 102,955 + -2.1 102,981 + -2.6 102,982 - -0.5 103,028 + -3.3 103,028 + -3.0 103,088 + -3.0 103,088 + -2.0 103,088 + -1.7 103,206 + Pf6N2E2_63 0.14 -1.8 103,207 - Pf6N2E2_63 0.14 -1.3
Or see this region's nucleotide sequence