Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6020 and Pf6N2E2_6021 are separated by 144 nucleotides Pf6N2E2_6021 and Pf6N2E2_6022 overlap by 11 nucleotides Pf6N2E2_6022 and Pf6N2E2_6023 are separated by 4 nucleotides
Pf6N2E2_6020 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 6,840,119 to 6,841,075
_6020
Pf6N2E2_6021 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 6,841,220 to 6,841,507
_6021
Pf6N2E2_6022 - StbE replicon stabilization toxin, at 6,841,497 to 6,841,808
_6022
Pf6N2E2_6023 - Putative cytoplasmic protein, at 6,841,813 to 6,842,451
_6023
Position (kb)
6841
6842 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.807 kb on - strand, within Pf6N2E2_6020 at 6841.033 kb on + strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.136 kb on - strand at 6841.198 kb on + strand at 6841.198 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.692 kb on + strand, within Pf6N2E2_6022 at 6841.787 kb on + strand at 6841.788 kb on - strand at 6841.865 kb on + strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.928 kb on - strand, within Pf6N2E2_6023 at 6842.161 kb on - strand, within Pf6N2E2_6023 at 6842.161 kb on - strand, within Pf6N2E2_6023 at 6842.194 kb on - strand, within Pf6N2E2_6023 at 6842.199 kb on + strand, within Pf6N2E2_6023 at 6842.199 kb on + strand, within Pf6N2E2_6023 at 6842.200 kb on - strand, within Pf6N2E2_6023 at 6842.218 kb on + strand, within Pf6N2E2_6023 at 6842.219 kb on - strand, within Pf6N2E2_6023 at 6842.332 kb on + strand, within Pf6N2E2_6023 at 6842.333 kb on - strand, within Pf6N2E2_6023 at 6842.362 kb on + strand, within Pf6N2E2_6023 at 6842.393 kb on - strand at 6842.523 kb on - strand at 6842.556 kb on + strand at 6842.559 kb on - strand at 6842.686 kb on + strand at 6842.686 kb on + strand at 6842.687 kb on - strand at 6842.705 kb on + strand at 6842.705 kb on + strand at 6842.707 kb on + strand at 6842.707 kb on + strand at 6842.707 kb on + strand at 6842.708 kb on - strand at 6842.708 kb on - strand at 6842.724 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 6,840,647 + Pf6N2E2_6020 0.55 +0.1 6,840,647 + Pf6N2E2_6020 0.55 +1.7 6,840,647 + Pf6N2E2_6020 0.55 -1.8 6,840,647 + Pf6N2E2_6020 0.55 -1.1 6,840,647 + Pf6N2E2_6020 0.55 +1.0 6,840,807 - Pf6N2E2_6020 0.72 -0.7 6,841,033 + -0.0 6,841,034 - -1.4 6,841,034 - -0.4 6,841,034 - -0.6 6,841,135 + +1.2 6,841,135 + +0.2 6,841,135 + -0.7 6,841,135 + +1.0 6,841,135 + +1.2 6,841,135 + +0.1 6,841,135 + +0.5 6,841,136 - +1.6 6,841,198 + +0.4 6,841,198 + +2.5 6,841,509 + -1.1 6,841,509 + -2.5 6,841,509 + -0.2 6,841,509 + +0.6 6,841,509 + +1.6 6,841,653 + Pf6N2E2_6022 0.50 -0.2 6,841,653 + Pf6N2E2_6022 0.50 -1.7 6,841,692 + Pf6N2E2_6022 0.62 -1.1 6,841,787 + -1.2 6,841,788 - -1.2 6,841,865 + +0.9 6,841,866 - +0.4 6,841,866 - +0.3 6,841,866 - +0.6 6,841,866 - +1.9 6,841,928 - Pf6N2E2_6023 0.18 +0.2 6,842,161 - Pf6N2E2_6023 0.54 +0.7 6,842,161 - Pf6N2E2_6023 0.54 -0.5 6,842,194 - Pf6N2E2_6023 0.60 +0.5 6,842,199 + Pf6N2E2_6023 0.60 +0.6 6,842,199 + Pf6N2E2_6023 0.60 +1.9 6,842,200 - Pf6N2E2_6023 0.61 -0.1 6,842,218 + Pf6N2E2_6023 0.63 -1.3 6,842,219 - Pf6N2E2_6023 0.64 +3.7 6,842,332 + Pf6N2E2_6023 0.81 +0.5 6,842,333 - Pf6N2E2_6023 0.81 +0.3 6,842,362 + Pf6N2E2_6023 0.86 -0.9 6,842,393 - +1.7 6,842,523 - +1.2 6,842,556 + +0.3 6,842,559 - -2.5 6,842,686 + +0.5 6,842,686 + +0.1 6,842,687 - +0.1 6,842,705 + -0.3 6,842,705 + +0.8 6,842,707 + -1.2 6,842,707 + +1.8 6,842,707 + +0.1 6,842,708 - -0.4 6,842,708 - -0.3 6,842,724 + +1.3
Or see this region's nucleotide sequence